FastQCFastQC Report
Fri 10 Feb 2017
SRR3224547_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224547_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556130
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG5610.10087569453185406No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5900.037.089591
ATGCGGG905.700167E-736.9367878
CCGTGCG702.0512204E-433.9306959
GTATAAT1551.3315002E-930.6912461
AAATGCG1252.1561391E-730.3909656
GTATCAA8200.029.006971
ACCGTGC908.952544E-426.3834198
CCCCTAG1109.371748E-525.9480511
TCGCACC2402.237357E-1023.7857151
AGACCGC1801.952867E-723.7429436
ATAATAA3850.023.4345915
ATCAACG9750.023.3776663
TCAACGC10150.022.9242214
GTACATG18650.022.7016471
AGGCCGA1050.002198600222.6123286
AATGCGG1503.0365421E-522.160087
GTTATGC1503.0365421E-522.160083
TACATGG18950.022.0514932
TTACACG1954.2254214E-721.9165634
TATCAAC10800.021.5445212
Produced by FastQC (version 0.11.5)