##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224547_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556130
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.146553863305343	34.0	31.0	34.0	28.0	34.0
2	31.313766565371406	34.0	31.0	34.0	28.0	34.0
3	31.456598277381186	34.0	31.0	34.0	28.0	34.0
4	34.556375307931596	37.0	35.0	37.0	32.0	37.0
5	34.55652455361157	37.0	35.0	37.0	32.0	37.0
6	34.66159710858972	37.0	37.0	37.0	32.0	37.0
7	34.639370291118986	37.0	37.0	37.0	32.0	37.0
8	34.66469530505457	37.0	37.0	37.0	32.0	37.0
9	36.31653390394332	39.0	38.0	39.0	32.0	39.0
10-11	36.39528437595526	39.0	38.0	39.0	32.0	39.0
12-13	36.41078614712387	39.0	38.0	39.0	32.0	39.0
14-15	37.84507129627965	41.0	39.0	41.0	32.0	41.0
16-17	37.69731537590132	41.0	39.0	41.0	31.5	41.0
18-19	37.77418679085825	41.0	39.0	41.0	32.0	41.0
20-21	37.831367665833525	41.0	39.0	41.0	32.0	41.0
22-23	37.79577796558358	41.0	39.0	41.0	32.0	41.0
24-25	37.744252243180554	41.0	39.0	41.0	32.0	41.0
26-27	37.58992142125042	41.0	39.0	41.0	31.0	41.0
28-29	37.519339003470414	41.0	39.0	41.0	31.0	41.0
30-31	37.464696204124934	41.0	39.0	41.0	30.5	41.0
32-33	37.29367144372719	41.0	38.5	41.0	30.0	41.0
34-35	37.304792045025444	41.0	38.0	41.0	30.0	41.0
36-37	37.322503731142	41.0	38.0	41.0	30.0	41.0
38-39	37.23963102152374	41.0	38.0	41.0	30.0	41.0
40-41	37.17286425835686	40.0	38.0	41.0	30.0	41.0
42-43	37.06948735008002	40.0	38.0	41.0	29.0	41.0
44-45	36.94849945156708	40.0	38.0	41.0	28.5	41.0
46-47	36.84687752863539	40.0	38.0	41.0	28.0	41.0
48-49	36.69536798949886	40.0	37.5	41.0	27.5	41.0
50-51	35.53675129915668	39.0	35.5	40.0	25.5	40.5
52-53	35.881545681765054	39.5	36.5	40.5	26.0	40.5
54-55	36.37996781328107	40.0	36.5	41.0	27.0	41.0
56-57	36.29389441317677	40.0	36.0	41.0	26.0	41.0
58-59	36.19727311959434	40.0	35.5	41.0	26.0	41.0
60-61	35.9983807742794	40.0	35.0	41.0	26.0	41.0
62-63	35.75070127488178	39.0	35.0	41.0	26.0	41.0
64-65	35.50790552568644	39.0	35.0	41.0	25.0	41.0
66-67	35.26031323611386	38.5	35.0	41.0	25.0	41.0
68-69	34.9312849513603	37.5	35.0	40.0	24.5	41.0
70-71	34.59414165752612	37.0	35.0	40.0	24.0	41.0
72-73	34.21882473522378	36.5	35.0	39.0	23.5	41.0
74-75	33.854268786075195	36.0	34.5	39.0	23.0	40.5
76-77	33.430471292683364	35.5	34.0	38.0	22.0	39.0
78-79	33.10026073040476	35.0	34.0	37.0	22.0	39.0
80-81	32.71732418679086	35.0	34.0	37.0	21.0	39.0
82-83	32.406433747505076	35.0	34.0	36.0	20.0	37.0
84-85	32.14391059644328	35.0	34.0	36.0	20.0	37.0
86-87	31.8735151852984	35.0	33.5	35.5	20.0	36.5
88-89	31.686372790534588	35.0	33.0	35.0	19.5	36.0
90-91	31.505679427471993	35.0	33.0	35.0	19.0	36.0
92-93	31.334386744106595	35.0	33.0	35.0	18.0	36.0
94-95	31.19359502274648	35.0	33.0	35.0	18.0	35.0
96-97	31.03170841350044	35.0	32.5	35.0	17.0	35.0
98-99	30.94892021649614	35.0	32.0	35.0	17.0	35.0
100-101	29.792464891302394	34.0	29.5	35.0	13.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	24.0
7	2046.0
8	9984.0
9	6234.0
10	3815.0
11	3277.0
12	2820.0
13	2580.0
14	2071.0
15	1871.0
16	1599.0
17	1592.0
18	1555.0
19	1552.0
20	1631.0
21	1903.0
22	2255.0
23	2254.0
24	2477.0
25	2593.0
26	2755.0
27	3023.0
28	3056.0
29	3707.0
30	4239.0
31	5352.0
32	6703.0
33	9533.0
34	15116.0
35	25621.0
36	54217.0
37	115882.0
38	206158.0
39	46624.0
40	11.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.82289021671403	18.6607454789715	13.113954049566537	26.402410254747938
2	16.14496610504738	20.136838508981715	37.37111826371532	26.347077122255584
3	20.967759336845702	24.12565407368781	27.08809091399493	27.81849567547156
4	14.367683814935356	17.471274701958176	36.221387085753335	31.939654397353134
5	15.451423228381852	36.38843435887292	32.628342294067934	15.531800118677289
6	29.95900239152716	36.02700807365184	19.244960710625215	14.769028824195782
7	28.071494075126317	30.298491359933827	21.52626184525201	20.103752719687844
8	25.836045528923098	34.06613561577329	20.861669034218618	19.236149821084997
9	26.171218959595777	16.872853469512524	20.197076223185224	36.758851347706475
10-11	25.454749788718466	25.776886699153074	27.324186790858253	21.444176721270207
12-13	26.14766331613112	23.306780788664522	27.454372179166743	23.091183716037616
14-15	23.290143976514777	24.907811139652182	25.00836445269497	26.793680431138068
16-17	23.125349206934747	27.77892243174888	26.006728166075394	23.089000195240978
18-19	23.202321662071185	27.263881319685385	26.801185652027677	22.732611366215753
20-21	24.136237635161024	26.08250241495491	26.46286905637773	23.318390893506336
22-23	24.061370655693313	26.39488781790325	26.133628898978984	23.410112627424446
24-25	23.540340108465664	26.638472668217545	26.3581812802761	23.46300594304069
26-27	23.280797655224497	27.50184309424056	26.483376189020554	22.733983061514394
28-29	23.149083847301892	27.343067268444425	26.728193048387965	22.779655835865714
30-31	23.402262061028896	26.930933414849044	26.736824123855936	22.929980400266125
32-33	23.0834517109309	26.92266196752558	26.745724920432274	23.24816140111125
34-35	23.43543775735889	26.984787729487707	26.60115440634384	22.978620106809558
36-37	23.047642171144922	26.64715298010863	27.14281860364735	23.1623862450991
38-39	23.100694481907865	26.706533740455818	26.857226603943907	23.335545173692406
40-41	23.205578797548306	26.36657972273247	27.255979306084903	23.17186217363432
42-43	23.293634880931616	26.693687748265177	27.118514535819415	22.89416283498379
44-45	23.010522189032535	26.317071986447576	27.27253926263751	23.399866561882376
46-47	23.0130545016453	26.191448042723824	27.190944563321523	23.604552892309353
48-49	22.639041231366765	26.619135813568768	27.32032078830489	23.42150216675957
50-51	22.557405642565588	26.675957060399547	27.150486397065432	23.616150899969433
52-53	22.87936273892795	26.643230899250174	26.83293474547318	23.644471616348696
54-55	22.856796072860664	26.377735421574094	27.127739916925897	23.637728588639348
56-57	22.670328879938147	26.562943915990868	27.05608400913456	23.710643194936434
58-59	23.00588451107482	26.27509729585854	27.13969958779647	23.579318605270164
60-61	22.667239787022243	26.459096382237707	26.947927279659652	23.9257365510804
62-63	22.65398973506688	26.084477503752446	27.221768061434915	24.03976469974576
64-65	23.237982842848442	25.756056139883764	26.878547983625417	24.127413033642373
66-67	22.702494663028954	26.2422508259491	26.682337368509085	24.372917142512865
68-69	22.719508028698325	26.362631039505153	27.32778307230324	23.590077859493285
70-71	23.027709348533616	26.299426393109528	26.981011633970475	23.691852624386385
72-73	22.994623559239745	26.303652023807384	27.141405786416843	23.560318630536024
74-75	23.03723949436283	26.150899969431606	27.11362451225433	23.698236023951235
76-77	23.146206822145903	26.08670634563861	26.477082696491827	24.29000413572366
78-79	23.314060139558304	25.545374433493993	27.042928566290197	24.097636860657506
80-81	23.085635224923582	25.91105609640687	26.914994485535548	24.088314193133996
82-83	23.120536115858595	25.928038139785915	27.131960061167582	23.81946568318791
84-85	23.295188827892854	25.542309103598125	27.197386124081763	23.965115944427257
86-87	23.215460001061516	26.03351693973734	26.76380443983854	23.987218619362604
88-89	23.384876618990702	25.63340861117332	26.882912829700302	24.09880194013568
90-91	23.555823278729793	25.481811806591985	26.891464225990326	24.070900688687896
92-93	23.435190413338464	25.587657748702586	27.01130719682938	23.965844641129568
94-95	23.73428874543722	25.362145541510078	27.131246291334758	23.772319421717945
96-97	23.908348767374534	25.46994407782353	26.85882797187708	23.762879182924856
98-99	23.409512044471896	25.361945528580275	26.98567681993216	24.24286560701567
100-101	24.030355800055407	25.035889428179768	26.589928652896162	24.343826118868662
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.5
5	1.0
6	2.0
7	1.5
8	0.5
9	1.0
10	3.5
11	3.5
12	4.0
13	6.5
14	5.5
15	6.0
16	7.0
17	10.5
18	12.5
19	12.5
20	19.0
21	24.0
22	30.0
23	37.5
24	37.5
25	53.0
26	83.5
27	99.5
28	115.0
29	158.5
30	180.0
31	236.5
32	342.0
33	501.0
34	942.0
35	1915.0
36	3675.0
37	6680.0
38	11079.0
39	16589.5
40	22470.0
41	27925.5
42	32755.5
43	36497.0
44	38675.5
45	39421.5
46	38289.0
47	34920.0
48	31739.0
49	29335.0
50	26111.0
51	21975.5
52	18196.5
53	15775.5
54	14196.0
55	12919.5
56	11163.5
57	9765.5
58	9113.5
59	8541.5
60	7448.0
61	5956.5
62	4814.5
63	3663.5
64	2632.5
65	1949.5
66	1616.5
67	1419.0
68	1078.5
69	759.5
70	539.0
71	387.5
72	296.5
73	222.5
74	166.5
75	138.0
76	112.5
77	66.5
78	44.0
79	35.5
80	24.5
81	19.0
82	13.5
83	6.0
84	6.0
85	6.5
86	5.5
87	7.0
88	7.0
89	3.5
90	1.5
91	1.5
92	2.5
93	4.0
94	2.5
95	3.0
96	3.0
97	2.0
98	1.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15032456440040998
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03704169888335461
16-17	0.07309442036933811
18-19	0.026792296765144843
20-21	0.03937928182259543
22-23	0.06464315897362127
24-25	0.018251128333303362
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.019779547947422364
38-39	0.005754050311977415
40-41	0.004405444770107709
42-43	0.003776095517235179
44-45	0.012047542840702714
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.016902522791433658
60-61	0.019689640911297716
62-63	0.02868034452376243
64-65	0.0482800783989355
66-67	0.018520849441677305
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01618326650243648
80-81	0.05736068904752486
82-83	0.05034794022980238
84-85	0.0312876485713772
86-87	0.05781022422814809
88-89	0.03407476669124126
90-91	0.0
92-93	0.004315537733983061
94-95	0.0
96-97	0.0
98-99	6.293492528725298E-4
100-101	0.04603240249581932
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	556130.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.91948372770762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.29027129949854	47.08483424739908
2	12.931427904736301	15.755518236372145
3	4.418306200797474	8.074827981105342
4	1.969213467398647	4.798538711342983
5	1.0522394004929467	3.205094051799145
6	0.6196397178610719	2.2648879025567346
7	0.3769359153772054	1.607391895424717
8	0.2620856208367414	1.2772896571064014
9	0.19396221053105958	1.0634469955413375
>10	0.7849826731496083	8.395683945993945
>50	0.067983094103986	2.855280305849484
>100	0.0323587565639314	3.419716492592396
>500	5.937386525490402E-4	0.19748957691629876
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	561	0.10087569453185406	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.394422167478827E-4	0.0
2	0.0	0.0	0.0	7.192562889971769E-4	0.0
3	0.0	0.0	0.0	7.192562889971769E-4	0.0
4	0.0	0.0	0.0	0.0010788844334957653	0.0
5	0.0	0.0	0.0	0.0010788844334957653	0.0
6	0.0	0.0	0.0	0.0010788844334957653	0.0
7	0.0	0.0	0.0	0.0012586985057450597	0.0
8	0.0	0.0	0.0	0.0012586985057450597	0.0
9	0.0	0.0	0.0	0.0017981407224929424	0.0
10-11	0.0	0.0	0.0	0.0021577688669915307	0.0
12-13	0.0	0.0	0.0	0.0021577688669915307	0.0
14-15	0.0	0.0	0.0	0.0021577688669915307	0.0
16-17	0.0	0.0	0.0	0.002337582939240825	0.0
18-19	0.0	0.0	0.0	0.002966932192113355	0.0
20-21	0.0	0.0	0.0	0.003776095517235179	0.0
22-23	0.0	0.0	0.0	0.006653120673223887	0.0
24-25	0.0	0.0	0.0	0.011418193587830184	0.0
26-27	0.0	0.0	0.0	0.02013917609192095	0.0
28-29	0.0	0.0	8.990703612464712E-5	0.03191699782424973	0.0
30-31	0.0	0.0	1.7981407224929423E-4	0.07057702335784799	0.0
32-33	0.0	0.0	1.7981407224929423E-4	0.16641792386672183	0.0
34-35	0.0	0.0	1.7981407224929423E-4	0.2911189829716073	0.0
36-37	0.0	0.0	1.7981407224929423E-4	0.481272364375236	0.0
38-39	0.0	0.0	1.7981407224929423E-4	0.8008918777983565	0.0
40-41	0.0	0.0	1.7981407224929423E-4	1.1816481757862372	0.0
42-43	0.0	0.0	1.7981407224929423E-4	1.517720676820168	0.0
44-45	0.0	0.0	1.7981407224929423E-4	1.8827432434862352	0.0
46-47	0.0	0.0	1.7981407224929423E-4	2.273569129520076	0.0
48-49	0.0	0.0	1.7981407224929423E-4	2.6656537140596623	0.0
50-51	0.0	0.0	1.7981407224929423E-4	3.06879686404258	0.0
52-53	0.0	0.0	1.7981407224929423E-4	3.453059536439322	0.0
54-55	0.0	0.0	1.7981407224929423E-4	3.8430762591480407	0.0
56-57	0.0	0.0	1.7981407224929423E-4	4.256648625321418	0.0
58-59	0.0	0.0	1.7981407224929423E-4	4.684426303202489	0.0
60-61	0.0	0.0	1.7981407224929423E-4	5.13638897380109	0.0
62-63	0.0	0.0	1.7981407224929423E-4	5.5923974610253	0.0
64-65	0.0	0.0	1.7981407224929423E-4	6.019995324834122	0.0
66-67	0.0	0.0	1.7981407224929423E-4	6.486792656393289	0.0
68-69	0.0	0.0	1.7981407224929423E-4	6.992250013486055	0.0
70-71	0.0	0.0	1.7981407224929423E-4	7.515059428550878	0.0
72-73	0.0	0.0	1.7981407224929423E-4	8.08507003758114	0.0
74-75	0.0	0.0	1.7981407224929423E-4	8.63125528203837	0.0
76-77	0.0	0.0	1.7981407224929423E-4	9.16170679517379	0.0
78-79	0.0	0.0	1.7981407224929423E-4	9.74385485408088	0.0
80-81	0.0	0.0	1.7981407224929423E-4	10.353334651969863	0.0
82-83	0.0	0.0	1.7981407224929423E-4	10.990865445129735	0.0
84-85	0.0	0.0	1.7981407224929423E-4	11.6112239943898	0.0
86-87	0.0	0.0	1.7981407224929423E-4	12.233650405480734	0.0
88-89	0.0	0.0	1.7981407224929423E-4	12.875137108230089	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	590	0.0	37.08959	1
ATGCGGG	90	5.700167E-7	36.936787	8
CCGTGCG	70	2.0512204E-4	33.930695	9
GTATAAT	155	1.3315002E-9	30.691246	1
AAATGCG	125	2.1561391E-7	30.390965	6
GTATCAA	820	0.0	29.00697	1
ACCGTGC	90	8.952544E-4	26.383419	8
CCCCTAG	110	9.371748E-5	25.948051	1
TCGCACC	240	2.237357E-10	23.785715	1
AGACCGC	180	1.952867E-7	23.742943	6
ATAATAA	385	0.0	23.434591	5
ATCAACG	975	0.0	23.377666	3
TCAACGC	1015	0.0	22.924221	4
GTACATG	1865	0.0	22.701647	1
AGGCCGA	105	0.0021986002	22.612328	6
AATGCGG	150	3.0365421E-5	22.16008	7
GTTATGC	150	3.0365421E-5	22.16008	3
TACATGG	1895	0.0	22.051493	2
TTACACG	195	4.2254214E-7	21.916563	4
TATCAAC	1080	0.0	21.544521	2
>>END_MODULE