FastQCFastQC Report
Fri 10 Feb 2017
SRR3224547_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224547_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556130
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT162342.9191016488950425No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT67591.2153633143329796RNA PCR Primer, Index 46 (95% over 21bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG33760.6070523079136173RNA PCR Primer, Index 46 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT27790.49970330678078867RNA PCR Primer, Index 46 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC11680.21002283638717564No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT9260.16650783090284646No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT9210.16560876054159998RNA PCR Primer, Index 46 (96% over 26bp)
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT6620.11903691582903278No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC6360.11436174995055114No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG6180.11112509665006383No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA6100.10968658407206947RNA PCR Primer, Index 46 (96% over 28bp)
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG6060.1089673277830723No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT5630.10123532267635264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5250.042.7207641
GTATCAA9350.039.8092041
GTATAAT1553.45608E-1133.865811
ATCAACG11550.032.8923383
TCAACGC11600.032.341184
TATCAAC13100.028.6379892
CAACGCA14150.026.5129095
AACGCAG14300.026.2348026
CGCAGAG14700.025.520938
ACGCAGA15000.025.3270997
GTATAGT1701.0716212E-725.263531
ATGCGGG1000.001654944823.7441568
TATACTG2002.0545485E-823.7441565
TATAGTA1652.4313285E-623.0246355
TAGGACC2704.0017767E-1122.8647424
TATTAGC1252.2576282E-422.794392
TATAGGA2303.6270649E-922.71182
AATGCGG1050.00219794822.6134827
TTCGGTC550.002875411421.59336358-59
CGATTAT2205.640686E-821.5855962
Produced by FastQC (version 0.11.5)