##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224547_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556130
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.095594555229894	34.0	33.0	34.0	31.0	34.0
2	33.248344811464946	34.0	33.0	34.0	31.0	34.0
3	33.36370812579793	34.0	34.0	34.0	31.0	34.0
4	36.57913797133764	37.0	37.0	37.0	35.0	37.0
5	36.60882887094744	37.0	37.0	37.0	35.0	37.0
6	36.65638969305738	37.0	37.0	37.0	35.0	37.0
7	36.6439681369464	37.0	37.0	37.0	35.0	37.0
8	36.62150036861885	37.0	37.0	37.0	35.0	37.0
9	38.48576591804075	39.0	39.0	39.0	37.0	39.0
10-11	38.50892147519465	39.0	39.0	39.0	37.0	39.0
12-13	38.518127955693814	39.0	39.0	39.0	37.0	39.0
14-15	40.09943718195386	41.0	40.0	41.0	38.0	41.0
16-17	40.08548360994732	41.0	40.0	41.0	38.0	41.0
18-19	40.09176451549098	41.0	40.0	41.0	38.0	41.0
20-21	40.11713178573355	41.0	40.0	41.0	38.0	41.0
22-23	40.04266628306331	41.0	40.0	41.0	38.0	41.0
24-25	40.01005969827199	41.0	40.0	41.0	38.0	41.0
26-27	39.95574236239728	41.0	40.0	41.0	38.0	41.0
28-29	39.90932605685721	41.0	40.0	41.0	38.0	41.0
30-31	39.855266754176185	41.0	40.0	41.0	38.0	41.0
32-33	39.82836656896769	41.0	40.0	41.0	38.0	41.0
34-35	39.73531368564904	41.0	40.0	41.0	37.5	41.0
36-37	39.619308435078125	41.0	40.0	41.0	37.0	41.0
38-39	39.480375991225074	41.0	40.0	41.0	37.0	41.0
40-41	39.46268498372683	41.0	39.5	41.0	37.0	41.0
42-43	39.46257439807239	41.0	39.5	41.0	36.5	41.0
44-45	39.29317965223959	41.0	39.0	41.0	36.0	41.0
46-47	39.14738460431913	41.0	39.0	41.0	35.0	41.0
48-49	39.11080952295326	41.0	39.0	41.0	35.0	41.0
50-51	38.842531422509126	40.0	38.5	41.0	35.0	41.0
52-53	38.70327441425566	40.0	38.0	41.0	35.0	41.0
54-55	38.59293870138277	40.0	37.0	41.0	35.0	41.0
56-57	38.36023411792207	40.0	37.0	41.0	34.5	41.0
58-59	38.09716073579918	40.0	36.0	41.0	34.0	41.0
60-61	37.780882167838456	40.0	36.0	41.0	33.5	41.0
62-63	37.47749357164692	39.5	35.0	41.0	33.0	41.0
64-65	37.11071062521353	39.0	35.0	41.0	33.0	41.0
66-67	36.60028950065632	39.0	35.0	41.0	31.5	41.0
68-69	36.003284304029634	38.0	35.0	40.5	30.5	41.0
70-71	35.32086292773272	37.0	35.0	40.0	28.5	41.0
72-73	34.80902396921583	37.0	35.0	39.0	28.0	41.0
74-75	34.4191142358801	36.0	35.0	39.0	27.0	40.5
76-77	32.60319529606387	34.5	32.0	36.5	25.0	39.0
78-79	33.751435815366904	35.0	34.5	37.0	27.5	39.0
80-81	33.55791271824933	35.0	35.0	37.0	28.0	39.0
82-83	33.27687321309766	35.0	35.0	36.5	27.0	37.5
84-85	33.01898476974808	35.0	34.5	36.0	27.0	37.0
86-87	32.78126966716415	35.0	34.0	36.0	26.5	37.0
88-89	32.59909373707586	35.0	34.0	35.0	26.0	36.0
90-91	32.45901407944186	35.0	34.0	35.0	26.0	36.0
92-93	32.36842374984266	35.0	34.0	35.0	25.5	36.0
94-95	32.263809720748746	35.0	34.0	35.0	25.0	35.5
96-97	32.211401111250964	35.0	34.0	35.0	25.0	35.0
98-99	32.17300990775538	35.0	34.0	35.0	25.0	35.0
100-101	31.385993382842145	34.5	32.5	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	0.0
14	7.0
15	16.0
16	44.0
17	89.0
18	159.0
19	290.0
20	434.0
21	677.0
22	998.0
23	1403.0
24	2234.0
25	4886.0
26	6280.0
27	10589.0
28	14408.0
29	7730.0
30	3927.0
31	4165.0
32	4922.0
33	6458.0
34	9868.0
35	18632.0
36	45129.0
37	116181.0
38	234623.0
39	61961.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.97954825491691	20.068726535597236	14.233655767500514	25.718069441985335
2	14.173844245050619	19.88042364195422	42.61018107277075	23.33555104022441
3	18.73087227806448	26.38537751964469	30.938269828996816	23.945480373294014
4	12.312049340981424	20.01798140722493	37.292179885997875	30.377789365795767
5	13.49522593638178	36.90755758545664	35.81500728246992	13.782209195691655
6	27.444482405193032	38.404329922859766	19.512344236059914	14.638843435887292
7	25.65443331595131	31.510797835038566	21.50036861884811	21.33440023016201
8	24.080700555625484	36.12572599931671	22.14176541456134	17.651808030496465
9	24.390879830255514	16.598457195260103	19.616456583892255	39.39420639059213
10-11	23.84595328430403	26.331343390933775	29.018035351446603	20.804667973315592
12-13	25.218384190746768	22.64623379425674	28.554204952079548	23.581177062916943
14-15	22.65126858827972	25.030928020426877	24.51225432902379	27.805549062269613
16-17	23.246722888533256	28.90726988294104	25.330857892938702	22.515149335587004
18-19	23.109344937334797	26.907287864348262	26.59575998417636	23.38760721414058
20-21	23.040213045436687	26.341176586939458	25.751111934266635	24.86749843335722
22-23	23.553665880567465	27.88032830955106	25.34607994835661	23.219925861524864
24-25	24.033226163760858	28.054872259623682	25.784351803901263	22.127549772714193
26-27	23.42552940890876	29.100609495689294	26.359092670497407	21.11476842490454
28-29	22.14367019296646	26.860748021513846	27.9632336942351	23.03234809128459
30-31	22.794307379565872	28.648762370228916	26.346485987375107	22.21044426283011
32-33	25.4721934518672	26.42323350020139	26.639730421773404	21.464842626158006
34-35	23.616725528134822	26.475163097815535	26.938652204248097	22.96945916980155
36-37	21.78087943486865	28.493927103665857	26.91773425987447	22.807459201591026
38-39	22.337582939240825	25.653264524481685	27.762213870857533	24.246938665419957
40-41	21.905309909553523	25.783809540934673	28.90043694819557	23.41044360131624
42-43	23.54863071583982	25.426069444194706	29.15514358153669	21.870156258428786
44-45	21.674213352418835	26.44642812186429	28.742262998701655	23.137095527015216
46-47	23.714940973343758	24.744949601735396	27.187261290911756	24.35284813400909
48-49	22.837311755450663	24.7337379186334	27.994425713643516	24.43452461227242
50-51	21.698164098322334	25.121554312840523	28.87157678959956	24.30870479923759
52-53	21.915219386851444	25.76153575740902	30.037428332789396	22.285816522950142
54-55	21.490928380055024	25.817165051336914	28.697426860626113	23.99447970798195
56-57	23.04168348818118	26.244979396079906	26.905311882118422	23.80802523362049
58-59	21.339766023487595	27.762022843605223	28.918144197672106	21.98006693523508
60-61	22.388069097502015	29.79532994704731	25.76002575687809	22.05657519857258
62-63	20.83793357668171	31.005879920162556	25.78470861129592	22.37147789185982
64-65	20.973962922338302	30.960207145811232	25.46032402495819	22.605505906892272
66-67	20.881898117346665	30.955442072896627	25.489004369481954	22.673655440274757
68-69	21.203675399636776	30.581248988545845	25.813388955819683	22.4016866559977
70-71	21.520238073831656	30.00368618848111	25.645712333447218	22.830363404240018
72-73	21.40830381385647	29.350511571035547	25.97063636200169	23.270548253106288
74-75	21.749770737057883	28.81547479905777	26.044809666804525	23.38994479707982
76-77	21.89639795155072	28.497486190229587	25.645085304102654	23.961030554117038
78-79	21.929438083437784	28.108056589081766	25.967353559476265	23.995151768004185
80-81	21.79495479165318	28.280842018946505	25.984099753278233	23.940103436122083
82-83	21.79254341642002	28.05362453832405	26.331352966773718	23.822479078482214
84-85	22.067818401686694	27.50237135137808	26.402783584404375	24.02702666253085
86-87	21.977774980669988	27.71087695323036	26.191088414579326	24.12025965152033
88-89	22.40033805045583	27.135471922032618	26.08553755416899	24.378652473342562
90-91	22.280671785373922	27.059140848362794	26.128603024472696	24.531584341790587
92-93	22.220813677389888	26.936891497394043	26.36912805380761	24.473166771408458
94-95	22.12522251991441	26.787441785194112	26.41270925862658	24.6746264362649
96-97	22.261341772607125	26.66516821606459	26.419452286335925	24.65403772499236
98-99	21.81180659198389	26.40425799723086	26.78950964702498	24.994425763760272
100-101	22.191253590806813	26.420960865266363	26.393430971490368	24.994354572436457
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	0.0
6	0.5
7	1.5
8	3.5
9	2.5
10	1.5
11	2.0
12	3.0
13	5.5
14	6.0
15	6.0
16	7.0
17	8.0
18	10.0
19	13.0
20	14.5
21	20.0
22	26.5
23	34.0
24	39.0
25	39.0
26	57.0
27	81.0
28	94.0
29	119.0
30	149.0
31	198.0
32	284.5
33	557.5
34	1276.5
35	2789.0
36	5605.5
37	10018.5
38	15800.5
39	22414.0
40	28683.0
41	34182.5
42	37992.0
43	40369.5
44	41590.5
45	40659.5
46	37929.5
47	34202.0
48	30575.5
49	27725.0
50	24724.0
51	19790.5
52	14841.5
53	12172.0
54	10863.5
55	9791.0
56	8288.0
57	7134.5
58	6822.5
59	6575.0
60	5538.0
61	4136.0
62	3268.5
63	2382.0
64	1550.5
65	1067.5
66	842.0
67	751.5
68	577.5
69	379.5
70	274.5
71	208.5
72	162.0
73	115.5
74	89.5
75	69.5
76	44.0
77	28.0
78	19.0
79	9.5
80	6.0
81	3.5
82	1.0
83	1.5
84	2.5
85	1.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47327063816014237
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.09163325121824E-4
22-23	0.001528419614119001
24-25	3.5962814449858846E-4
26-27	0.0026073040476147663
28-29	0.004135723661733768
30-31	0.0011687914696204124
32-33	3.5962814449858846E-4
34-35	0.003955909589484473
36-37	0.002067861830866884
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.006473306600974592
46-47	0.07309442036933811
48-49	8.990703612464711E-4
50-51	0.0
52-53	8.990703612464712E-5
54-55	0.0
56-57	0.008361354359592182
58-59	0.03946918885872008
60-61	0.029129879704385665
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	3.5962814449858846E-4
78-79	0.009170517684714006
80-81	0.00683293474547318
82-83	7.192562889971769E-4
84-85	0.002247675903116178
86-87	0.0
88-89	0.0
90-91	0.0
92-93	8.990703612464712E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06662111376836351
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	556130.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.245763155809456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78266714132134	39.286144196107216
2	14.756010790597966	15.71390111361496
3	5.095034502584374	8.138670011858553
4	2.3067604670496675	4.913008859428439
5	1.2192304805271892	3.245942869924724
6	0.7130803644000705	2.2781104916342674
7	0.46133909634198467	1.7195046580838333
8	0.33292499799486036	1.418147647350614
9	0.2252801666738485	1.0795692958575316
>10	0.9830386850593625	9.145056651742951
>50	0.07992788284300642	2.952385714854971
>100	0.04030261763747748	3.6432352288438765
>500	0.0027094196731077187	0.999396606358101
>1k	0.0010160323774153946	1.320566825759721
>5k	3.3867745913846483E-4	1.218859917425912
>10k+	3.3867745913846483E-4	2.927499911154351
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	16234	2.9191016488950425	No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	6759	1.2153633143329796	RNA PCR Primer, Index 46 (95% over 21bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG	3376	0.6070523079136173	RNA PCR Primer, Index 46 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT	2779	0.49970330678078867	RNA PCR Primer, Index 46 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	1168	0.21002283638717564	No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT	926	0.16650783090284646	No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT	921	0.16560876054159998	RNA PCR Primer, Index 46 (96% over 26bp)
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	662	0.11903691582903278	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC	636	0.11436174995055114	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	618	0.11112509665006383	No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA	610	0.10968658407206947	RNA PCR Primer, Index 46 (96% over 28bp)
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	606	0.1089673277830723	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	563	0.10123532267635264	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.7981407224929423E-4	0.0
2	0.0	0.0	0.0	3.5962814449858846E-4	0.0
3	0.0	0.0	0.0	3.5962814449858846E-4	0.0
4	0.0	0.0	0.0	7.192562889971769E-4	0.0
5	0.0	0.0	0.0	7.192562889971769E-4	0.0
6	0.0	0.0	0.0	7.192562889971769E-4	0.0
7	0.0	0.0	0.0	7.192562889971769E-4	0.0
8	0.0	0.0	0.0	7.192562889971769E-4	0.0
9	0.0	0.0	0.0	0.0014385125779943538	0.0
10-11	0.0	0.0	0.0	0.0018880477586175894	0.0
12-13	0.0	0.0	0.0	0.0021577688669915307	0.0
14-15	0.0	0.0	0.0	0.002337582939240825	0.0
16-17	0.0	0.0	0.0	0.0025173970114901194	0.0
18-19	0.0	0.0	0.0	0.003146746264362649	0.0
20-21	0.0	0.0	0.0	0.003955909589484473	0.0
22-23	0.0	0.0	0.0	0.007012748817722475	0.0
24-25	0.0	0.0	0.0	0.011957635804578065	0.0
26-27	0.0	0.0	0.0	0.021038246453167424	0.0
28-29	0.0	0.0	0.0	0.0328160681854962	0.0
30-31	0.0	0.0	0.0	0.07210544297196698	0.0
32-33	0.0	0.0	0.0	0.17046374049233093	0.0
34-35	0.0	0.0	0.0	0.2967831262474601	0.0
36-37	0.0	0.0	0.0	0.4874759498678367	0.0
38-39	0.0	0.0	0.0	0.809163325121824	0.0
40-41	0.0	0.0	0.0	1.191807670868322	0.0
42-43	0.0	0.0	0.0	1.5296783126247462	0.0
44-45	0.0	0.0	0.0	1.8966788340855554	0.0
46-47	0.0	0.0	0.0	2.290921187492133	0.0
48-49	0.0	0.0	0.0	2.6854332620070847	0.0
50-51	0.0	0.0	0.0	3.091902972326614	0.0
52-53	0.0	0.0	0.0	3.4795821120960926	0.0
54-55	0.0	1.7981407224929423E-4	0.0	3.872296045888551	0.0
56-57	0.0	1.7981407224929423E-4	0.0	4.291083020157158	0.0
58-59	0.0	1.7981407224929423E-4	0.0	4.72236707244709	0.0
60-61	0.0	1.7981407224929423E-4	0.0	5.182151655188535	0.0
62-63	0.0	1.7981407224929423E-4	0.0	5.645442612338842	0.0
64-65	0.0	1.7981407224929423E-4	0.0	6.076636757592649	0.0
66-67	0.0	1.7981407224929423E-4	0.0	6.550716559077914	0.0
68-69	0.0	1.7981407224929423E-4	0.0	7.063995828313524	0.0
70-71	0.0	1.7981407224929423E-4	0.0	7.591300595184579	0.0
72-73	0.0	1.7981407224929423E-4	0.0	8.172279862622048	0.0
74-75	0.0	1.7981407224929423E-4	0.0	8.730782371028358	0.0
76-77	0.0	1.7981407224929423E-4	0.0	9.271932821462608	0.0
78-79	0.0	1.7981407224929423E-4	0.0	9.858666139212055	0.0
80-81	0.0	1.7981407224929423E-4	0.0	10.477765989966375	0.0
82-83	0.0	1.7981407224929423E-4	0.0	11.12383795155809	0.0
84-85	0.0	1.7981407224929423E-4	0.0	11.75228813406937	0.0
86-87	0.0	1.7981407224929423E-4	0.0	12.388290507615125	0.0
88-89	0.0	1.7981407224929423E-4	0.0	13.042004567277434	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	525	0.0	42.720764	1
GTATCAA	935	0.0	39.809204	1
GTATAAT	155	3.45608E-11	33.86581	1
ATCAACG	1155	0.0	32.892338	3
TCAACGC	1160	0.0	32.34118	4
TATCAAC	1310	0.0	28.637989	2
CAACGCA	1415	0.0	26.512909	5
AACGCAG	1430	0.0	26.234802	6
CGCAGAG	1470	0.0	25.52093	8
ACGCAGA	1500	0.0	25.327099	7
GTATAGT	170	1.0716212E-7	25.26353	1
ATGCGGG	100	0.0016549448	23.744156	8
TATACTG	200	2.0545485E-8	23.744156	5
TATAGTA	165	2.4313285E-6	23.024635	5
TAGGACC	270	4.0017767E-11	22.864742	4
TATTAGC	125	2.2576282E-4	22.79439	2
TATAGGA	230	3.6270649E-9	22.7118	2
AATGCGG	105	0.002197948	22.613482	7
TTCGGTC	55	0.0028754114	21.593363	58-59
CGATTAT	220	5.640686E-8	21.585596	2
>>END_MODULE