FastQCFastQC Report
Fri 10 Feb 2017
SRR3224546_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224546_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161094
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA3980.24706072231119716No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG3880.24085316647423244No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA3510.21788520987746285No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC2860.17753609693719194No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA2760.1713285411002272No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC2310.1433945398338858No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA2150.1334624504947422No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG1980.12290960557190213No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT1930.11980582765341974No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA1880.11670204973493736No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC1840.11421902740015145No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT1820.11297751623275852No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC1770.10987373831427615No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA1750.10863222714688318No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA1680.10428693806100785No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC1660.1030454268936149No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA1620.10056240455882899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG353.558671E-881.470949
TACACCC352.7906723E-454.246515
AACCGTG451.5165737E-552.7396627
TCGTACC405.360523E-447.5247159
ACCGTGC652.5772479E-643.841718
AAACCGT554.9653652E-543.150636
TGCGCGG308.252108E-539.60392812-13
TCTACAC608.294249E-539.5547453
CTCGTAC500.001612491937.996158
CCTCGTA500.001617422737.9725577
GTATCAA2800.037.3292431
GGTATCA1302.0190782E-1036.5461121
GTCTAAT1106.9361704E-834.552691
CGCACCA1950.034.0779342
CGGCTTA352.0438038E-433.94622416-17
TCAACGC3250.032.1306234
ACGTGTT300.003936724731.68314222-23
ATCAACG3300.031.6437973
TCGCACC2000.030.8814661
ACAAGAC1401.6985723E-830.513663
Produced by FastQC (version 0.11.5)