FastQCFastQC Report
Fri 10 Feb 2017
SRR3224546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161094
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT2770.17194929668392367No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG2540.15767191825890475No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA2480.1539473847567259No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG2320.1440152954175823No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT2240.13904925074801047No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC2220.13780773958061754No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG2140.13284169491104572No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA2130.13222093932734924No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC2040.12663413907408097No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA2030.1260133834903845No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT1980.12290960557190213No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT1830.113598271816455No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG1780.11049449389797261No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG1710.10614920481209729No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG1660.1030454268936149No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAA200.002072817971.2327659
GGTATCA1350.063.5547941
GTAATAC451.5122654E-552.7650153
CCGTGCG851.2732926E-1050.2819569
ACCGTGC1006.311893E-1042.739668
AAACCGT1051.0186341E-940.7044376
GTATCAA2950.038.7791981
TGTAGAG756.8967493E-637.990812
CACCGTG500.001613608837.990817
AACCGTG1152.4792826E-937.164927
CTTAATA905.6682256E-736.9355132
GTATCAT550.002529372234.666251
GTATATT550.002529372234.666251
TATAACT851.6262291E-533.5213054
GTACATG6200.033.0587431
ACACCGT600.003945627231.6590126
ATCAACG3700.030.803363
TCAACGC3850.029.6032284
AGTAATA650.005836250729.2237012
CATGGGG5150.028.585324
Produced by FastQC (version 0.11.5)