##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224546_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	161094
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.16451885234708	34.0	33.0	34.0	31.0	34.0
2	33.29066880206587	34.0	34.0	34.0	31.0	34.0
3	33.41596831663501	34.0	34.0	34.0	31.0	34.0
4	36.629613765875824	37.0	37.0	37.0	35.0	37.0
5	36.66221584913156	37.0	37.0	37.0	35.0	37.0
6	36.70063441220654	37.0	37.0	37.0	36.0	37.0
7	36.68257042472097	37.0	37.0	37.0	36.0	37.0
8	36.669304877897375	37.0	37.0	37.0	36.0	37.0
9	38.5169280047674	39.0	39.0	39.0	37.0	39.0
10-11	38.54125231231455	39.0	39.0	39.0	37.5	39.0
12-13	38.53362632996884	39.0	39.0	39.0	37.0	39.0
14-15	40.14378561585161	41.0	40.0	41.0	38.0	41.0
16-17	40.15828336250885	41.0	40.0	41.0	38.0	41.0
18-19	40.148388518504724	41.0	40.0	41.0	38.5	41.0
20-21	40.15621624641514	41.0	40.0	41.0	38.5	41.0
22-23	40.08658919636982	41.0	40.0	41.0	38.0	41.0
24-25	40.056991570139175	41.0	40.0	41.0	38.0	41.0
26-27	39.99791115746086	41.0	40.0	41.0	38.0	41.0
28-29	39.963564751014935	41.0	40.0	41.0	38.0	41.0
30-31	39.89330453027425	41.0	40.0	41.0	38.0	41.0
32-33	39.841952524612964	41.0	40.0	41.0	38.0	41.0
34-35	39.766425192744606	41.0	40.0	41.0	37.0	41.0
36-37	39.651361937750636	41.0	40.0	41.0	37.0	41.0
38-39	39.543409437967895	41.0	40.0	41.0	37.0	41.0
40-41	39.41131885731312	41.0	39.5	41.0	36.0	41.0
42-43	39.32517660496356	41.0	39.0	41.0	35.5	41.0
44-45	39.08301054042981	41.0	39.0	41.0	35.0	41.0
46-47	38.8563012899301	41.0	38.5	41.0	35.0	41.0
48-49	38.74263163122152	40.0	38.0	41.0	35.0	41.0
50-51	38.61410418761717	40.0	38.0	41.0	35.0	41.0
52-53	38.37721144176692	40.0	37.0	41.0	35.0	41.0
54-55	38.19407302568686	40.0	37.0	41.0	35.0	41.0
56-57	37.98923609817871	40.0	36.0	41.0	34.5	41.0
58-59	37.727326902305485	40.0	35.5	41.0	34.0	41.0
60-61	37.50525779979391	39.0	35.0	41.0	34.0	41.0
62-63	37.30858380821135	39.0	35.0	41.0	34.0	41.0
64-65	37.075102114293514	38.5	35.0	41.0	33.5	41.0
66-67	36.82336089488125	38.0	35.0	41.0	33.0	41.0
68-69	36.58710131972637	37.0	35.0	40.5	33.0	41.0
70-71	36.31696711236917	37.0	35.0	40.0	33.0	41.0
72-73	36.03144747787006	36.0	35.0	39.5	33.0	41.0
74-75	35.72290712254957	36.0	35.0	39.0	32.5	41.0
76-77	33.86110904192583	34.5	32.5	36.5	29.5	39.0
78-79	35.15804437160912	35.0	35.0	37.5	32.5	39.0
80-81	35.05723056103889	35.0	35.0	37.0	33.0	39.0
82-83	34.80678051324072	35.0	35.0	37.0	33.0	39.0
84-85	34.56262492706122	35.0	35.0	36.0	33.0	37.5
86-87	34.33324021999578	35.0	35.0	36.0	32.0	37.0
88-89	34.155986566849165	35.0	35.0	36.0	32.0	37.0
90-91	33.98445938396216	35.0	35.0	35.0	32.0	36.0
92-93	33.86411349895092	35.0	35.0	35.0	32.0	36.0
94-95	33.74188362074317	35.0	34.5	35.0	32.0	36.0
96-97	33.67999739282655	35.0	34.0	35.0	31.0	36.0
98-99	33.632022297540566	35.0	34.0	35.0	31.0	35.5
100-101	32.82758203285039	34.5	33.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	6.0
15	11.0
16	11.0
17	49.0
18	103.0
19	145.0
20	225.0
21	332.0
22	429.0
23	430.0
24	370.0
25	433.0
26	532.0
27	709.0
28	944.0
29	1064.0
30	1193.0
31	1309.0
32	1537.0
33	2111.0
34	3391.0
35	7104.0
36	20044.0
37	39729.0
38	53142.0
39	25726.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.92611120120757	20.9459712328938	13.191576951385336	22.93634061451329
2	15.32769687263337	20.862353656871143	40.34042236209915	23.469527108396342
3	18.875315033458726	28.254931903112467	29.72550188088942	23.14425118253939
4	12.596372304368877	17.48171874806014	40.51671694786895	29.405191999702033
5	12.303996424447838	39.48750419010019	35.54881001154605	12.65968937390592
6	28.12829776403839	39.09146212770184	19.63201608998473	13.148224018275045
7	25.181571008231217	32.41585658062994	24.29699430146374	18.105578109675097
8	25.03507269047885	35.911331272424796	21.199423938818327	17.854172098278024
9	26.272238568785927	15.800712627410082	21.406135548189255	36.52091325561474
10-11	24.814083702682908	25.957205110059967	29.243485170149103	19.985226017108022
12-13	25.351037282580357	24.242367810098454	28.806473239226786	21.600121668094406
14-15	22.598607024470184	26.136293095956397	26.034489180230175	25.23061069934324
16-17	21.88411734763554	29.051671694786897	27.189715321489317	21.874495636088245
18-19	22.078103467540693	27.931518244006604	28.27883099308478	21.71154729536792
20-21	23.022415623273513	26.923267925980642	28.031013141477285	22.02330330926856
22-23	23.212440057730806	27.415615252106708	27.52611077486537	21.84583391529711
24-25	22.740830635624654	27.066268968021678	27.318917274750376	22.873983121603292
26-27	22.363789860515354	27.900653659687265	27.85875239768333	21.87680408211406
28-29	22.10613876628738	27.753257475588335	28.304188315920815	21.836415442203474
30-31	22.534979577141296	26.904788508572636	28.024631581561078	22.535600332724993
32-33	22.507045575874955	27.247755968564935	28.465057668193726	21.78014078736638
34-35	23.114623328170538	27.186298214956373	27.53672670612738	22.162351750745707
36-37	22.297473462039854	26.887143832640138	28.20162642001366	22.613756285306348
38-39	22.40058599327101	27.307348504599798	27.61462251852955	22.67744298359964
40-41	22.534669199349448	27.033284914397804	28.132953430916114	22.299092455336638
42-43	22.412380349361243	27.227891789886648	28.184786522154763	22.17494133859734
44-45	22.709382236266226	26.990458216673595	27.76429249880495	22.535867048255227
46-47	22.778880532801526	26.7811046809621	27.63131327251096	22.808701513725417
48-49	22.329578784991323	27.084270935822985	27.620605424799884	22.965544854385808
50-51	22.457385129179237	26.95351782189281	27.61369138515401	22.975405663773945
52-53	22.709411896160006	27.307038126807953	27.152780364259378	22.830769612772666
54-55	22.771177076737807	26.68348914298484	27.51281860280333	23.03251517747402
56-57	22.97749896791376	27.13129689008359	27.041901149418152	22.8493029925845
58-59	23.279240338096745	26.60058750830647	27.401735198951677	22.718436954645107
60-61	22.896964127147747	27.372532460677718	27.185002576983504	22.54550083519104
62-63	22.791041255416093	26.86692241796715	27.617415918656192	22.72462040796057
64-65	22.967025463394044	26.621103206823342	27.19623325511813	23.215638074664483
66-67	22.535600332724993	27.07301327175438	26.957863110978685	23.433523284541945
68-69	22.945609395756513	26.717630700088147	27.54664978211479	22.790110122040545
70-71	23.19142860690032	26.750530746024058	27.247135212981238	22.81090543409438
72-73	22.898121593603733	26.83029783852906	27.42467130991843	22.846909257948774
74-75	22.635541981700126	26.80360534843011	27.394254286317306	23.16659838355246
76-77	22.604193824723453	26.331831104820786	27.38959861943958	23.674376451016176
78-79	22.788986838837843	26.15160168860194	27.300720139061337	23.75869133349888
80-81	22.209702951857714	26.962473228419782	27.162988484340566	23.66483533538194
82-83	22.321026739295746	27.24149168955724	27.30139516116517	23.136086409981843
84-85	22.495468315454907	26.618990862137466	27.51291219705999	23.372628625347637
86-87	21.863011657789862	27.686319788446497	27.594758339851268	22.855910213912374
88-89	22.77521198803183	26.38614721839423	26.868474306926394	23.97016648664755
90-91	22.64392218208003	26.72942505617838	26.8749922405552	23.751660521186388
92-93	22.633990092740884	26.76729114678387	27.31293530485307	23.285783455622184
94-95	22.73486287509156	26.441704843135067	27.02862924752008	23.79480303425329
96-97	22.631507070406098	26.74897885706482	27.154642631010468	23.464871441518618
98-99	21.51973382000571	27.269792791786163	27.71052925621066	23.499944131997466
100-101	22.204757674219223	26.909624070426997	27.135144538117927	23.750473717235852
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	1.0
3	1.5
4	0.5
5	0.5
6	1.0
7	2.0
8	4.5
9	7.5
10	7.5
11	6.0
12	6.0
13	6.0
14	6.0
15	8.5
16	12.0
17	18.5
18	20.5
19	14.5
20	20.5
21	31.0
22	33.5
23	38.0
24	55.0
25	81.0
26	120.0
27	168.0
28	237.5
29	412.0
30	850.0
31	1571.0
32	2566.5
33	3812.5
34	5154.0
35	6499.0
36	7633.5
37	8136.5
38	7934.5
39	7230.0
40	6315.0
41	5375.0
42	4708.0
43	4295.0
44	3928.0
45	3861.5
46	3960.0
47	4070.0
48	4210.5
49	4746.0
50	5517.5
51	5514.0
52	5170.0
53	5426.0
54	5890.5
55	5801.0
56	4881.0
57	4212.0
58	4099.0
59	4041.5
60	3417.5
61	2461.5
62	1980.0
63	1418.5
64	856.0
65	550.0
66	422.0
67	362.5
68	289.0
69	202.0
70	118.5
71	77.0
72	58.5
73	43.5
74	29.0
75	23.5
76	18.0
77	13.0
78	7.0
79	2.0
80	3.5
81	5.5
82	3.0
83	1.0
84	0.5
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47922331061367895
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	6.207555836964753E-4
22-23	9.311333755447131E-4
24-25	3.1037779184823767E-4
26-27	6.207555836964753E-4
28-29	0.0018622667510894261
30-31	0.0
32-33	0.0
34-35	0.0034141557103306145
36-37	0.0024830223347859014
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.00806982258805418
46-47	0.08287087042347946
48-49	3.1037779184823767E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.007759444796205941
58-59	0.04655666877723565
60-61	0.03290004593591319
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.008690578171750655
80-81	0.005586800253268278
82-83	9.311333755447131E-4
84-85	0.0037245335021788522
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.08256049263163122
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	161094.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.44588873576918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.17398119122258	43.344258631606394
2	11.862964621585311	13.155052329695705
3	3.8524406627854906	6.408059890498715
4	1.8092252575011194	4.012564093014016
5	1.0143304970891176	2.812022794145033
6	0.6907747424988804	2.298037170844352
7	0.4982086878638603	1.9336536432145206
8	0.30228392297357815	1.3408320607843867
9	0.24070756829377518	1.2011620544526798
>10	1.3849081952530229	14.777086669894596
>50	0.12091356918943125	4.508547804387501
>100	0.04926108374384236	4.208722857462103
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	277	0.17194929668392367	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	254	0.15767191825890475	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	248	0.1539473847567259	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	232	0.1440152954175823	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	224	0.13904925074801047	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	222	0.13780773958061754	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	214	0.13284169491104572	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	213	0.13222093932734924	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	204	0.12663413907408097	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	203	0.1260133834903845	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	198	0.12290960557190213	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	183	0.113598271816455	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	178	0.11049449389797261	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	171	0.10614920481209729	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	166	0.1030454268936149	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	3.1037779184823767E-4	0.0
20-21	0.0	0.0	0.0	6.207555836964753E-4	0.0
22-23	0.0	0.0	0.0	6.207555836964753E-4	0.0
24-25	0.0	0.0	0.0	0.0018622667510894261	0.0
26-27	0.0	0.0	0.0	0.002793400126634139	0.0
28-29	0.0	0.0	0.0	0.0040349112940270895	0.0
30-31	0.0	0.0	0.0	0.008690578171750655	0.0
32-33	0.0	0.0	0.0	0.02079531205383192	0.0
34-35	0.0	0.0	0.0	0.03631420164624381	0.0
36-37	0.0	0.0	0.0	0.06300669174519224	0.0
38-39	0.0	0.0	0.0	0.09683787105665015	0.0
40-41	0.0	0.0	0.0	0.13967000633170695	0.0
42-43	0.0	0.0	0.0	0.1846747861497014	0.0
44-45	0.0	0.0	0.0	0.24302581101717008	0.0
46-47	0.0	0.0	0.0	0.3196891256036848	0.0
48-49	0.0	0.0	0.0	0.39231752889617244	0.0
50-51	0.0	0.0	0.0	0.4606006431027847	0.0
52-53	0.0	0.0	0.0	0.5329186686034242	0.0
54-55	0.0	0.0	0.0	0.6160999168187518	0.0
56-57	0.0	0.0	0.0	0.7188349659205184	0.0
58-59	0.0	0.0	0.0	0.8315021043614288	0.0
60-61	0.0	0.0	0.0	0.9485145318882143	0.0
62-63	0.0	0.0	0.0	1.056526003451401	0.0
64-65	0.0	0.0	0.0	1.189678076154295	0.0
66-67	0.0	0.0	0.0	1.342383949743628	0.0
68-69	0.0	0.0	0.0	1.5177474021378823	0.0
70-71	0.0	0.0	0.0	1.717320322296299	0.0
72-73	0.0	0.0	0.0	1.9113064422014476	0.0
74-75	0.0	0.0	0.0	2.111189740151713	0.0
76-77	0.0	0.0	0.0	2.323177771984059	0.0
78-79	0.0	0.0	0.0	2.587619650638757	0.0
80-81	0.0	0.0	0.0	2.8663389077184753	0.0
82-83	0.0	0.0	0.0	3.1518864762188534	0.0
84-85	0.0	0.0	0.0	3.4569878456056715	0.0
86-87	0.0	0.0	0.0	3.7980930388468845	0.0
88-89	0.0	0.0	0.0	4.141060498839187	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCGAA	20	0.0020728179	71.232765	9
GGTATCA	135	0.0	63.554794	1
GTAATAC	45	1.5122654E-5	52.765015	3
CCGTGCG	85	1.2732926E-10	50.281956	9
ACCGTGC	100	6.311893E-10	42.73966	8
AAACCGT	105	1.0186341E-9	40.704437	6
GTATCAA	295	0.0	38.779198	1
TGTAGAG	75	6.8967493E-6	37.99081	2
CACCGTG	50	0.0016136088	37.99081	7
AACCGTG	115	2.4792826E-9	37.16492	7
CTTAATA	90	5.6682256E-7	36.935513	2
GTATCAT	55	0.0025293722	34.66625	1
GTATATT	55	0.0025293722	34.66625	1
TATAACT	85	1.6262291E-5	33.521305	4
GTACATG	620	0.0	33.058743	1
ACACCGT	60	0.0039456272	31.659012	6
ATCAACG	370	0.0	30.80336	3
TCAACGC	385	0.0	29.603228	4
AGTAATA	65	0.0058362507	29.223701	2
CATGGGG	515	0.0	28.58532	4
>>END_MODULE