FastQCFastQC Report
Fri 10 Feb 2017
SRR3224544_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224544_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882236
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG352.7838335E-454.3005269
GTATCAA13400.037.2598841
GGTATCA7950.037.0835721
ATCAACG15100.032.7037543
TCAACGC15350.032.171124
TATCAAC16750.029.1987082
TAGACCG856.4191455E-427.9313745
AACGCAG18200.027.1333376
CGCAGAG18450.026.767198
CAACGCA18350.026.6527735
AACGGGT1301.0044931E-525.5679515
ACGCAGA19350.025.2753687
CATAACG950.001228839624.991232
ACATGGG29300.022.8503443
GTATAGG4200.022.6431941
TCCGCTA3450.022.0225078
ATGCGGG1302.9466132E-421.916638
TACATGG31800.021.6512072
GACGTAT1100.00288175721.5845588
GTACATG31900.021.46491
Produced by FastQC (version 0.11.5)