##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224544_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	882236
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.870317012681415	34.0	31.0	34.0	30.0	34.0
2	32.0291271269819	34.0	31.0	34.0	31.0	34.0
3	32.16793238997275	34.0	31.0	34.0	31.0	34.0
4	35.35085509999592	37.0	35.0	37.0	35.0	37.0
5	35.37728566959408	37.0	37.0	37.0	35.0	37.0
6	35.486576154226306	37.0	37.0	37.0	35.0	37.0
7	35.46145589162084	37.0	37.0	37.0	35.0	37.0
8	35.48549481091227	37.0	37.0	37.0	35.0	37.0
9	37.216878476960815	39.0	39.0	39.0	35.0	39.0
10-11	37.2947017578063	39.0	39.0	39.0	35.0	39.0
12-13	37.31946327286576	39.0	39.0	39.0	35.0	39.0
14-15	38.821412864584985	41.0	40.0	41.0	36.0	41.0
16-17	38.67250146219379	41.0	40.0	41.0	36.0	41.0
18-19	38.7803994622754	41.0	40.0	41.0	36.0	41.0
20-21	38.85359926368908	41.0	40.0	41.0	36.0	41.0
22-23	38.81743773774818	41.0	40.0	41.0	36.0	41.0
24-25	38.78983117895892	41.0	40.0	41.0	36.0	41.0
26-27	38.65816289518904	41.0	40.0	41.0	36.0	41.0
28-29	38.599082898453474	41.0	39.5	41.0	35.0	41.0
30-31	38.54570262378774	41.0	39.0	41.0	35.0	41.0
32-33	38.379023866629794	41.0	39.0	41.0	35.0	41.0
34-35	38.39977511686216	41.0	39.0	41.0	35.0	41.0
36-37	38.42992634623842	41.0	39.0	41.0	35.0	41.0
38-39	38.3625141118703	41.0	39.0	41.0	35.0	41.0
40-41	38.32358971975752	41.0	39.0	41.0	35.0	41.0
42-43	38.23737242642558	41.0	39.0	41.0	34.5	41.0
44-45	38.14663310044024	41.0	39.0	41.0	34.0	41.0
46-47	38.06903821653163	41.0	39.0	41.0	34.0	41.0
48-49	37.960203959031375	40.0	39.0	41.0	33.0	41.0
50-51	36.81733855793688	39.0	37.0	40.0	31.5	40.5
52-53	37.178581467997226	39.5	37.5	40.5	32.5	41.0
54-55	37.753678154144694	40.0	38.0	41.0	33.0	41.0
56-57	37.72079012871839	40.0	38.0	41.0	33.0	41.0
58-59	37.675563568024884	40.0	38.0	41.0	33.0	41.0
60-61	37.526147198708735	40.0	37.5	41.0	33.0	41.0
62-63	37.30744948063783	40.0	37.0	41.0	32.0	41.0
64-65	37.120175893978484	40.0	36.5	41.0	32.0	41.0
66-67	36.91200710467494	39.5	36.0	41.0	31.5	41.0
68-69	36.62575603353298	39.0	35.5	41.0	31.0	41.0
70-71	36.30779349289759	39.0	35.0	41.0	31.0	41.0
72-73	35.94468600238485	38.0	35.0	40.0	30.5	41.0
74-75	35.573926931115935	37.0	35.0	39.5	30.0	41.0
76-77	35.11060022488314	37.0	35.0	39.0	29.5	41.0
78-79	34.73442140198314	36.0	35.0	39.0	29.0	39.5
80-81	34.28563955676259	36.0	35.0	37.5	29.0	39.0
82-83	33.895903137029094	35.0	34.5	37.0	29.0	39.0
84-85	33.54924759361441	35.0	34.0	37.0	29.0	38.0
86-87	33.21165085079276	35.0	34.0	36.0	28.0	37.0
88-89	32.94740012876373	35.0	34.0	36.0	27.0	37.0
90-91	32.70500126950159	35.0	34.0	35.5	26.5	36.0
92-93	32.49532438032454	35.0	34.0	35.0	26.0	36.0
94-95	32.304941081524674	35.0	34.0	35.0	25.0	36.0
96-97	32.11791912821513	35.0	33.5	35.0	25.0	36.0
98-99	32.007872610049915	35.0	33.0	35.0	24.5	35.0
100-101	30.822741307314594	34.5	31.0	35.0	20.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	12.0
7	1654.0
8	7653.0
9	4984.0
10	2979.0
11	2552.0
12	2513.0
13	2232.0
14	1916.0
15	1788.0
16	1717.0
17	1714.0
18	1767.0
19	1860.0
20	2101.0
21	2504.0
22	2898.0
23	2914.0
24	3028.0
25	3214.0
26	3626.0
27	3799.0
28	4450.0
29	5103.0
30	5964.0
31	7527.0
32	9822.0
33	13332.0
34	20478.0
35	34531.0
36	67450.0
37	154123.0
38	369760.0
39	130243.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.85425315855019	21.066045457693193	14.200959740618007	23.878741643138603
2	14.169904651363128	22.131266463848675	41.665722097035264	22.03310678775294
3	19.3928141513476	27.355100800921296	29.081060126905108	24.171024920825996
4	12.230627632515564	18.954338748362115	41.00694145330728	27.80809216581504
5	13.55317624762535	38.477912939394905	35.12620205931293	12.84270875366682
6	26.439410769907372	39.31453715332405	21.351316427803898	12.894735648964675
7	25.63271052190117	32.37784447698802	24.033025176936786	17.95641982417403
8	23.129525433104067	36.839235760046066	23.115130191921434	16.916108614928433
9	25.633277263680014	17.649925870175327	21.493455265937914	35.22334160020675
10-11	23.36580008070403	27.715599907507745	29.944992042945422	18.973607968842803
12-13	24.06311916539339	25.12230287587448	29.89959602646004	20.914981932272088
14-15	21.35172906455609	26.855537570550197	27.45741475154707	24.335318613346637
16-17	21.119610337534674	29.911517173359076	28.141690492165516	20.827181996940734
18-19	21.16610480023083	29.179489824898347	29.159252429479483	20.495152945391343
20-21	22.19085086491399	28.137386134729397	28.883268502344634	20.788494498011982
22-23	21.844514287204973	28.4261106567929	28.431611624849857	21.297763431152266
24-25	21.4200405288591	28.570294897048193	28.684115804837955	21.325548769254752
26-27	21.434060727515085	28.927690549920882	28.707114649594896	20.931134072969137
28-29	21.266815228578295	28.811395136902146	28.856734479209646	21.065055155309917
30-31	21.44329861851024	28.559478416206098	28.783171396315726	21.21405156896794
32-33	21.26834543138117	28.477244184095866	28.85849137872406	21.395919005798902
34-35	21.411504404717103	28.65038379753263	28.8307777057386	21.107334092011662
36-37	21.292262692624913	28.285004095537992	28.997429305912597	21.4253039059245
38-39	21.285498832740398	28.4327322376188	28.87141100970364	21.410357919937155
40-41	21.375901879991158	28.236310977856117	29.107840192249924	21.279946949902797
42-43	21.38511709836807	28.143224889396706	28.895032571720698	21.576625440514526
44-45	21.289259552316974	28.094286407403796	28.93065968970982	21.68579435056941
46-47	21.309830929592536	27.930961783468366	28.995472866670596	21.7637344202685
48-49	20.949949900026752	28.146493681962646	29.140728784588248	21.76282763342235
50-51	20.891235451738538	28.31385252925521	28.92429009924782	21.87062191975843
52-53	20.89066870995969	28.22498741833251	28.916865781944967	21.967478089762828
54-55	20.986788115651596	28.141619702664595	28.895329594348908	21.976262587334908
56-57	20.967518895170905	28.052074501607276	29.032311082295443	21.948095520926373
58-59	21.080611992717397	28.020455684263272	28.980095272428812	21.91883705059052
60-61	21.12896560988868	28.018437829292502	28.65289006512304	22.199706495695782
62-63	21.003084503840466	27.87068326777133	28.816045996012424	22.31018623237578
64-65	21.397054070696406	27.625996935906926	28.674105011527207	22.30284398186946
66-67	20.961529632029222	27.81249628016046	28.600903871410605	22.625070216399717
68-69	21.163214831405654	27.99812068426135	28.649930404109558	22.188734080223433
70-71	21.14060183442979	27.682842232690575	28.590592539864616	22.58596339301502
72-73	21.024023050521627	27.729825126156722	28.729444275681338	22.516707547640312
74-75	20.91679550596439	27.55328506204689	28.752737362791812	22.777182069196904
76-77	20.92767694811819	27.480175372576042	28.735905131960156	22.85624254734561
78-79	21.154097959225318	27.148204012849007	28.856530189895267	22.841167838030408
80-81	20.993406174651312	27.451986653571257	28.725216448498074	22.829390723279356
82-83	21.05669021671335	27.262562229958835	28.827241696056973	22.85350585727084
84-85	21.14007384391417	27.007167270127795	29.06585124300521	22.786907642952826
86-87	21.04464493300141	27.106160014063178	28.97480535535053	22.874389697584878
88-89	21.19838423924598	26.81584579406137	28.917411948125576	23.068358018567075
90-91	21.286877887549362	26.89116064182373	28.874926890310533	22.947034580316377
92-93	21.32706874516483	26.75861404488173	28.904537764641535	23.0097794453119
94-95	21.499802773860964	26.482483145099494	28.998589946454235	23.019124134585304
96-97	21.53346723552428	26.54374793139251	29.005674218689787	22.917110614393426
98-99	21.28774736307575	26.30639045011479	29.049721132969218	23.356141053840236
100-101	21.637940517082033	26.117019889253406	28.935580773230306	23.309458820434255
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.5
4	1.0
5	1.0
6	1.5
7	2.5
8	7.5
9	9.5
10	10.5
11	14.0
12	11.5
13	12.5
14	21.0
15	26.0
16	36.5
17	45.0
18	42.5
19	44.5
20	55.5
21	66.0
22	78.0
23	95.5
24	118.0
25	149.0
26	204.0
27	258.0
28	311.5
29	438.0
30	755.0
31	1506.5
32	2959.0
33	5718.0
34	10742.5
35	18626.5
36	29443.0
37	41440.5
38	52328.0
39	62470.5
40	69490.5
41	71024.0
42	68690.0
43	62982.0
44	55184.5
45	47655.0
46	40565.5
47	33666.0
48	28248.0
49	24130.0
50	20641.5
51	17246.5
52	14484.5
53	13103.0
54	12367.0
55	11734.5
56	10248.5
57	8917.0
58	8204.5
59	7629.0
60	6657.5
61	5216.0
62	4107.5
63	3068.0
64	2209.0
65	1584.0
66	1241.0
67	1072.0
68	812.5
69	553.5
70	389.5
71	270.0
72	185.5
73	140.5
74	107.5
75	89.0
76	77.5
77	49.0
78	32.0
79	27.0
80	17.5
81	13.0
82	10.0
83	6.0
84	2.0
85	2.0
86	3.0
87	2.5
88	3.0
89	3.0
90	4.0
91	3.5
92	1.5
93	1.5
94	0.5
95	1.0
96	3.5
97	4.0
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15562729247049542
2	0.0
3	2.2669671153750245E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03723493487003477
16-17	0.07395980213911017
18-19	0.023293087110478373
20-21	0.037121586514266024
22-23	0.06494860785549444
24-25	0.016832230831659554
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.020232681504722093
38-39	0.004703956764403176
40-41	0.0057807661442063115
42-43	0.0032871023172937855
44-45	0.010654745442262616
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.015188679673012665
60-61	0.015472050562434541
62-63	0.027940369696997175
64-65	0.04635947750941925
66-67	0.0160387923412783
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.016378837408584552
80-81	0.056900874595913115
82-83	0.048286399557488015
84-85	0.02726027956238467
86-87	0.05678752624014436
88-89	0.033834484196972235
90-91	0.0
92-93	0.00323042813940941
94-95	0.0
96-97	0.0
98-99	6.234159567281317E-4
100-101	0.04567938737480674
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	882236.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.86240321140995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.00310856355877	34.90523225248272
2	14.879957890112689	14.839009201711995
3	5.983430289299973	8.950446410173155
4	3.0041091176689103	5.991684004651207
5	1.7264334368159113	4.30420600720876
6	1.0691141971702012	3.1985161907006043
7	0.7080218840947258	2.4712570867063364
8	0.5190019062189012	2.0702945852301777
9	0.35033329953553227	1.572161421584193
>10	1.6106634019206256	13.889204773743899
>50	0.09400911370700264	3.238927905107399
>100	0.05089978663055636	4.300628163152264
>500	9.171132662668281E-4	0.26843199754727165
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.4004506730625364E-4	0.0
2	0.0	0.0	0.0	4.533934230750049E-4	0.0
3	0.0	0.0	0.0	4.533934230750049E-4	0.0
4	0.0	0.0	0.0	4.533934230750049E-4	0.0
5	0.0	0.0	0.0	4.533934230750049E-4	0.0
6	0.0	0.0	0.0	4.533934230750049E-4	0.0
7	0.0	0.0	0.0	4.533934230750049E-4	0.0
8	0.0	0.0	0.0	4.533934230750049E-4	0.0
9	0.0	0.0	0.0	9.067868461500098E-4	0.0
10-11	0.0	0.0	0.0	0.001983596225953146	0.0
12-13	0.0	0.0	0.0	0.002040270403837522	0.0
14-15	0.0	0.0	0.0	0.002436989649028151	0.0
16-17	0.0	0.0	0.0	0.0031170797836406585	0.0
18-19	0.0	0.0	0.0	0.004703956764403176	0.0
20-21	0.0	0.0	0.0	0.006290833745165692	0.0
22-23	0.0	0.0	0.0	0.010881442153800117	0.0
24-25	0.0	0.0	0.0	0.019835962259531464	0.0
26-27	0.0	0.0	0.0	0.030547381879678454	0.0
28-29	0.0	0.0	0.0	0.048343073735372395	0.0
30-31	0.0	0.0	0.0	0.10076668827841984	0.0
32-33	0.0	0.0	0.0	0.21400169569140232	0.0
34-35	0.0	0.0	0.0	0.3589175685417507	0.0
36-37	0.0	0.0	0.0	0.5803435815360063	0.0
38-39	0.0	0.0	0.0	0.9339337773566256	0.0
40-41	0.0	0.0	0.0	1.3387574299847207	0.0
42-43	0.0	0.0	0.0	1.7367801812666905	0.0
44-45	0.0	0.0	0.0	2.15344873707262	0.0
46-47	0.0	0.0	0.0	2.6085990596620405	0.0
48-49	0.0	0.0	0.0	3.075197566184105	0.0
50-51	0.0	0.0	0.0	3.5354485647831195	0.0
52-53	0.0	0.0	0.0	3.964358743012074	0.0
54-55	0.0	0.0	0.0	4.401373328678494	0.0
56-57	0.0	0.0	0.0	4.8944386762725625	0.0
58-59	0.0	0.0	0.0	5.385407079284908	0.0
60-61	0.0	0.0	0.0	5.903409065148101	0.0
62-63	0.0	0.0	0.0	6.413363317751712	0.0
64-65	0.0	0.0	0.0	6.893790323677565	0.0
66-67	0.0	0.0	0.0	7.411055545228261	0.0
68-69	0.0	0.0	0.0	7.972980018951845	0.0
70-71	0.0	0.0	0.0	8.556213983559955	0.0
72-73	0.0	0.0	0.0	9.146475546225727	0.0
74-75	0.0	0.0	0.0	9.706529772079126	0.0
76-77	0.0	0.0	0.0	10.285059779922832	0.0
78-79	0.0	0.0	0.0	10.909609220208651	0.0
80-81	0.0	0.0	0.0	11.562042356013585	0.0
82-83	0.0	0.0	0.0	12.253694022914503	0.0
84-85	0.0	0.0	0.0	12.900516415108882	0.0
86-87	0.0	0.0	0.0	13.55833359781283	0.0
88-89	0.0	0.0	0.0	14.27616873489633	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	35	2.7838335E-4	54.300526	9
GTATCAA	1340	0.0	37.259884	1
GGTATCA	795	0.0	37.083572	1
ATCAACG	1510	0.0	32.703754	3
TCAACGC	1535	0.0	32.17112	4
TATCAAC	1675	0.0	29.198708	2
TAGACCG	85	6.4191455E-4	27.931374	5
AACGCAG	1820	0.0	27.133337	6
CGCAGAG	1845	0.0	26.76719	8
CAACGCA	1835	0.0	26.652773	5
AACGGGT	130	1.0044931E-5	25.567951	5
ACGCAGA	1935	0.0	25.275368	7
CATAACG	95	0.0012288396	24.99123	2
ACATGGG	2930	0.0	22.850344	3
GTATAGG	420	0.0	22.643194	1
TCCGCTA	345	0.0	22.022507	8
ATGCGGG	130	2.9466132E-4	21.91663	8
TACATGG	3180	0.0	21.651207	2
GACGTAT	110	0.002881757	21.584558	8
GTACATG	3190	0.0	21.4649	1
>>END_MODULE