FastQCFastQC Report
Fri 10 Feb 2017
SRR3224544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882236
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT125181.4188947175132278No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT48070.5448655461803872RNA PCR Primer, Index 46 (95% over 21bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG26490.300259794431422RNA PCR Primer, Index 46 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT18060.20470713051836473RNA PCR Primer, Index 46 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC10310.1168621547975825No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGCG352.7902742E-454.2751247
CCGTGCG554.9680857E-543.1733979
GGTATCA9550.040.9444661
CTGTCGA550.002581770834.5387159
GTATCAA15050.033.268831
GGCGTCT600.00395423431.660498
ACTGTCG753.081397E-431.6604888
TCAACGC16700.029.5750684
ATCAACG16800.029.3990273
TGCGCCC350.00839843327.13910122-23
CAACGCA18850.026.2017845
TATCAAC19050.025.9266992
AACGCAG19450.025.637676
CGCAGAG21250.023.9129838
TGCGCGG500.001655874823.74536712-13
GTACATG34850.023.3979641
ACGCAGA21850.022.8216347
ACCGTGC1050.002198310622.6146358
ACATGGG33500.022.5403773
GTCCATA2554.674803E-1022.4401511
Produced by FastQC (version 0.11.5)