##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224544_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	882236
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.13612684134404	34.0	33.0	34.0	31.0	34.0
2	33.27362519779288	34.0	33.0	34.0	31.0	34.0
3	33.39298781731872	34.0	34.0	34.0	31.0	34.0
4	36.590890646040286	37.0	37.0	37.0	35.0	37.0
5	36.63343481789453	37.0	37.0	37.0	35.0	37.0
6	36.68142651172702	37.0	37.0	37.0	35.0	37.0
7	36.67120022306956	37.0	37.0	37.0	35.0	37.0
8	36.64906215570437	37.0	37.0	37.0	35.0	37.0
9	38.51939390367203	39.0	39.0	39.0	37.0	39.0
10-11	38.54591118476235	39.0	39.0	39.0	37.5	39.0
12-13	38.56010409912994	39.0	39.0	39.0	37.5	39.0
14-15	40.166303574100354	41.0	40.0	41.0	38.0	41.0
16-17	40.16322503275767	41.0	40.0	41.0	38.0	41.0
18-19	40.18277762412778	41.0	40.0	41.0	38.5	41.0
20-21	40.20883527763546	41.0	40.0	41.0	39.0	41.0
22-23	40.142371202263334	41.0	40.0	41.0	38.5	41.0
24-25	40.12625930023259	41.0	40.0	41.0	38.0	41.0
26-27	40.08291942292085	41.0	40.0	41.0	38.0	41.0
28-29	40.0468718120775	41.0	40.0	41.0	38.0	41.0
30-31	39.999792572508944	41.0	40.0	41.0	38.0	41.0
32-33	39.972308429943915	41.0	40.0	41.0	38.0	41.0
34-35	39.89550358407501	41.0	40.0	41.0	38.0	41.0
36-37	39.81200325083084	41.0	40.0	41.0	38.0	41.0
38-39	39.72821161231235	41.0	40.0	41.0	38.0	41.0
40-41	39.69169983995212	41.0	40.0	41.0	38.0	41.0
42-43	39.68349795292869	41.0	40.0	41.0	37.5	41.0
44-45	39.54278333688491	41.0	40.0	41.0	37.0	41.0
46-47	39.416049673783434	41.0	40.0	41.0	37.0	41.0
48-49	39.40997646888134	41.0	39.0	41.0	37.0	41.0
50-51	39.27720473886806	41.0	39.0	41.0	36.0	41.0
52-53	39.17658880390282	41.0	39.0	41.0	36.0	41.0
54-55	39.095441015782626	41.0	39.0	41.0	35.0	41.0
56-57	38.94952314346728	41.0	39.0	41.0	35.0	41.0
58-59	38.77147271251683	40.0	38.0	41.0	35.0	41.0
60-61	38.55617147792654	40.0	38.0	41.0	35.0	41.0
62-63	38.35774724676844	40.0	37.5	41.0	35.0	41.0
64-65	38.09620271673339	40.0	37.0	41.0	34.0	41.0
66-67	37.74167626349413	39.5	36.5	41.0	34.0	41.0
68-69	37.343825801712924	39.0	36.0	41.0	33.5	41.0
70-71	36.85765543460026	39.0	35.5	41.0	33.0	41.0
72-73	36.43852495250704	38.5	35.0	40.0	33.0	41.0
74-75	36.03680931179413	37.0	35.0	39.5	32.5	41.0
76-77	34.05836590209422	35.5	33.0	38.0	29.5	39.0
78-79	35.200002040270405	36.0	35.0	39.0	32.0	39.5
80-81	34.96935797224325	36.0	35.0	37.5	32.0	39.0
82-83	34.62435901504813	35.5	35.0	37.0	32.0	39.0
84-85	34.295715092106875	35.0	35.0	37.0	32.0	38.0
86-87	33.99799600107001	35.0	35.0	36.0	31.5	37.0
88-89	33.75686834361781	35.0	35.0	36.0	31.0	37.0
90-91	33.56329258837771	35.0	35.0	36.0	31.0	36.5
92-93	33.43349001854379	35.0	35.0	35.0	31.0	36.0
94-95	33.307546960223796	35.0	34.5	35.0	31.0	36.0
96-97	33.23171520998916	35.0	34.0	35.0	31.0	36.0
98-99	33.160627088443455	35.0	34.0	35.0	31.0	35.5
100-101	32.3909362120793	34.5	33.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	9.0
15	32.0
16	85.0
17	166.0
18	276.0
19	473.0
20	680.0
21	1001.0
22	1262.0
23	1669.0
24	2262.0
25	4392.0
26	5491.0
27	8975.0
28	12243.0
29	7629.0
30	4994.0
31	5639.0
32	7001.0
33	8986.0
34	13743.0
35	23824.0
36	53903.0
37	145832.0
38	409613.0
39	162034.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.888868262538764	21.173796234185254	14.41500950393881	24.522325999337173
2	13.283520509251492	21.866484704772873	43.61180001722895	21.238194768746684
3	18.31505402182636	27.951591184218284	30.672745161158694	23.060609632796666
4	11.381308402740311	20.312705443894828	40.78466532764476	27.521320825720103
5	12.721426012994256	38.14772917904053	36.41803327000939	12.712811537955831
6	25.4875112781614	40.24943439170471	21.152163366718202	13.110890963415683
7	24.56961629314605	32.60748824577551	23.719503624880417	19.103391836198025
8	22.439007249760834	37.69558258787898	23.288893221314932	16.576516941045252
9	24.576757239559484	17.423115810282056	21.13334753966059	36.86677941049787
10-11	22.55615277544784	27.943713473492355	30.369651657833053	19.130482093226757
12-13	23.468947084453593	24.925303433548393	30.11694149864662	21.488807983351393
14-15	21.05462710657919	26.91439705475632	26.973621570645495	25.05735426801899
16-17	21.059217714987827	30.509920248096883	27.600097932979384	20.83076410393591
18-19	21.102290090179952	28.938685340430453	28.88926545731528	21.069759112074323
20-21	21.601356566960657	28.300369406059893	28.372743069527456	21.725530957451994
22-23	21.388218865050145	29.186578465333067	27.930038176245485	21.4951644933713
24-25	21.606391516063802	29.336444381976623	28.139707015841637	20.91745708611794
26-27	21.442262570022375	29.792919663847258	28.37257911309903	20.392238653031335
28-29	20.79438497536569	28.612300209305214	29.20416978149624	21.38914503383286
30-31	21.103773002119066	29.49241608041454	28.43668051984265	20.967130397623745
32-33	22.461261716697773	28.21761107732695	28.593248167012288	20.727879038962996
34-35	21.433862556810805	28.56520228586456	28.78017511812737	21.220760039197263
36-37	20.589955555253287	29.420982771851435	28.693782382832378	21.2952792900629
38-39	20.778680534460168	27.921440521583797	29.262578267039657	22.03730067691638
40-41	20.67014948381159	27.996477133102708	29.675676349638874	21.657697033446834
42-43	21.354603530121192	27.747167424589335	29.745612285148194	21.152616760141278
44-45	20.578486539917748	28.28317637083354	29.466856502872478	21.67148058637623
46-47	21.55028471607795	27.343406157126974	28.81287007418499	22.293439052610086
48-49	21.002417745092817	27.26775535094709	29.441515618996018	22.288311284964074
50-51	20.333334844644742	27.638919744830183	29.70004624612915	22.32769916439592
52-53	20.23619530375092	28.059158773842825	30.332813442208206	21.37183248019804
54-55	20.273260216087305	27.86091249960328	29.560457745999933	22.305369538309478
56-57	21.04015348385104	27.9690878536349	28.738944260083716	22.25181440243035
58-59	20.091268540871273	28.664717094737174	29.797097866772198	21.446916497619352
60-61	20.70443973910899	29.719692395781074	28.002528409171433	21.573339455938502
62-63	19.89331652755045	30.227626168054805	28.07162709297739	21.80743021141735
64-65	19.992326316314454	30.238790981098028	27.909708966761727	21.859173735825788
66-67	19.912019006252297	30.282543446424764	27.733678970252857	22.07175857707008
68-69	20.26039517770755	30.087414251968863	27.727614833219228	21.924575737104355
70-71	20.432061262519326	29.80455343014794	27.46623352481649	22.29715178251624
72-73	20.20128401017415	29.63249062609098	27.748414256502794	22.417811107232076
74-75	20.38445495309645	29.293522368164528	27.76842024129598	22.553602437443043
76-77	20.550398505611042	29.195842143831534	27.63916808872028	22.614591261837145
78-79	20.73009820113833	28.95703657689173	27.613586512334937	22.699278709635003
80-81	20.522521082608	29.121005866686616	27.564018637824628	22.79245441288075
82-83	20.519014021845766	28.8709129870255	27.744790052032887	22.86528293909585
84-85	20.81746420377776	28.538815890892934	27.78027149813651	22.863448407192795
86-87	20.53611505311504	28.49316962808138	27.886472553829133	23.08424276497445
88-89	20.768365834085213	28.1666130151116	27.703414959262602	23.361606191540584
90-91	20.798346474186044	28.3124356748081	27.71463644648371	23.174581404522147
92-93	20.781117524109195	28.119913492534877	27.81891693379096	23.28005204956497
94-95	20.739688700075718	27.70041122783473	27.919003531934766	23.640896540154788
96-97	20.795116046046637	27.738326252839375	28.082905254376378	23.383652446737607
98-99	20.357081325178296	27.805655176166017	28.18922601208747	23.648037486568217
100-101	20.611695717392724	27.418100771566866	28.281257532063552	23.688945978976857
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.0
2	0.5
3	1.0
4	1.0
5	0.5
6	0.5
7	2.0
8	5.5
9	6.0
10	7.5
11	9.0
12	7.0
13	10.0
14	16.5
15	22.0
16	20.5
17	15.5
18	17.5
19	29.5
20	38.0
21	41.0
22	56.0
23	79.5
24	100.5
25	122.0
26	154.0
27	205.0
28	290.0
29	417.5
30	704.0
31	1450.0
32	3058.0
33	5914.0
34	11192.0
35	19468.0
36	30880.0
37	43370.5
38	54418.0
39	64917.5
40	71437.0
41	72163.5
42	69644.0
43	64117.5
44	56986.0
45	49706.0
46	42237.5
47	35094.0
48	29241.0
49	24842.0
50	21054.5
51	16895.5
52	13208.5
53	11159.5
54	10458.5
55	9871.5
56	8334.0
57	7107.0
58	6694.0
59	6394.0
60	5342.0
61	3881.0
62	2977.0
63	2042.5
64	1256.5
65	784.0
66	583.5
67	501.5
68	364.0
69	236.5
70	151.0
71	109.0
72	86.0
73	70.0
74	59.0
75	39.5
76	22.0
77	14.0
78	9.5
79	6.5
80	4.5
81	1.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47368277875761133
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.001020135201918761
22-23	0.0013601802692250146
24-25	4.533934230750049E-4
26-27	0.002040270403837522
28-29	0.003570473206715663
30-31	8.501126682656341E-4
32-33	1.7002253365312682E-4
34-35	0.003570473206715663
36-37	0.0019269220480687707
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.008161081615350087
46-47	0.07435652138430081
48-49	0.001020135201918761
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.006630878812471946
58-59	0.03774500247099415
60-61	0.02930054996622219
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.8337088942187807E-4
78-79	0.007594339836506332
80-81	0.005837440322090688
82-83	6.234159567281317E-4
84-85	0.0022669671153750246
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06670550736991009
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	882236.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.61877027745885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.93293799074837	30.53398322195348
2	16.224067217083817	14.802439906843956
3	6.7071464625767145	9.179153210806737
4	3.322870348877093	6.063410364288147
5	1.8995056946955524	4.332655696352066
6	1.1607231088957135	3.177045651627084
7	0.7680656741804318	2.4526748083907073
8	0.5507551564591668	2.0099818369309315
9	0.39711076300898895	1.6304134205172127
>10	1.8677063048162073	14.69552113968961
>50	0.10560227110993578	3.3344714765025776
>100	0.06101845823716574	4.792541549771873
>500	0.001245274655461138	0.40401777370402386
>1k	9.962197243689106E-4	1.169447397282204
>5k	0.0	0.0
>10k+	2.4905493109222764E-4	1.4222425453394183
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	12518	1.4188947175132278	No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	4807	0.5448655461803872	RNA PCR Primer, Index 46 (95% over 21bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG	2649	0.300259794431422	RNA PCR Primer, Index 46 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT	1806	0.20470713051836473	RNA PCR Primer, Index 46 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	1031	0.1168621547975825	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.4004506730625364E-4	0.0
2	0.0	0.0	0.0	4.533934230750049E-4	0.0
3	0.0	0.0	0.0	5.667417788437561E-4	0.0
4	0.0	0.0	0.0	5.667417788437561E-4	0.0
5	0.0	0.0	0.0	5.667417788437561E-4	0.0
6	0.0	0.0	0.0	5.667417788437561E-4	0.0
7	0.0	0.0	0.0	5.667417788437561E-4	0.0
8	0.0	0.0	0.0	5.667417788437561E-4	0.0
9	0.0	0.0	0.0	0.001020135201918761	0.0
10-11	0.0	0.0	0.0	0.0020969445817218975	0.0
12-13	0.0	0.0	0.0	0.0021536187596062732	0.0
14-15	0.0	0.0	0.0	0.003003731427871907	0.0
16-17	0.0	0.0	0.0	0.0036838215624844144	0.0
18-19	0.0	0.0	0.0	0.00515735018747818	0.0
20-21	0.0	0.0	0.0	0.0066875529903563215	0.0
22-23	0.0	0.0	0.0	0.01150485811052825	0.0
24-25	0.0	0.0	0.0	0.020459378216259594	0.0
26-27	0.0	0.0	0.0	0.03111412365852221	0.0
28-29	0.0	0.0	0.0	0.049193186403638026	0.0
30-31	0.0	0.0	0.0	0.10212686854764486	0.0
32-33	0.0	0.0	0.0	0.21706210129715858	0.0
34-35	0.0	0.0	0.0	0.362884760993657	0.0
36-37	0.0	0.0	0.0	0.5861243476802125	0.0
38-39	0.0	0.0	0.0	0.9426049265729352	0.0
40-41	0.0	0.0	0.0	1.3509990524077458	0.0
42-43	0.0	0.0	0.0	1.751515467516628	0.0
44-45	0.0	0.0	0.0	2.171244428928314	0.0
46-47	0.0	0.0	0.0	2.6292284604119533	0.0
48-49	0.0	0.0	0.0	3.1018344297897613	0.0
50-51	0.0	0.0	0.0	3.565939272484913	0.0
52-53	0.0	0.0	0.0	3.9996100816561553	0.0
54-55	0.0	0.0	0.0	4.442008714221592	0.0
56-57	0.0	0.0	0.0	4.938984580089681	0.0
58-59	0.0	0.0	0.0	5.436243816847194	0.0
60-61	0.0	0.0	0.0	5.960309939744015	0.0
62-63	0.0	0.0	0.0	6.477971880539901	0.0
64-65	0.0	0.0	0.0	6.962592775629197	0.0
66-67	0.0	0.0	0.0	7.485922134213521	0.0
68-69	0.0	0.0	0.0	8.053173980658237	0.0
70-71	0.0	0.0	0.0	8.641848666343247	0.0
72-73	0.0	0.0	0.0	9.241688165071476	0.0
74-75	0.0	0.0	0.0	9.809393404939268	0.0
76-77	0.0	0.0	0.0	10.39421424652814	0.0
78-79	0.0	0.0	0.0	11.025054520559124	0.0
80-81	0.0	0.0	0.0	11.683155074152495	0.0
82-83	0.0	0.0	0.0	12.381664316577425	0.0
84-85	0.0	0.0	0.0	13.034947565050622	0.0
86-87	0.0	0.0	0.0	13.702909425595873	0.0
88-89	0.0	0.0	0.0	14.426922048068771	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATAGCG	35	2.7902742E-4	54.275124	7
CCGTGCG	55	4.9680857E-5	43.173397	9
GGTATCA	955	0.0	40.944466	1
CTGTCGA	55	0.0025817708	34.538715	9
GTATCAA	1505	0.0	33.26883	1
GGCGTCT	60	0.003954234	31.66049	8
ACTGTCG	75	3.081397E-4	31.660488	8
TCAACGC	1670	0.0	29.575068	4
ATCAACG	1680	0.0	29.399027	3
TGCGCCC	35	0.008398433	27.139101	22-23
CAACGCA	1885	0.0	26.201784	5
TATCAAC	1905	0.0	25.926699	2
AACGCAG	1945	0.0	25.63767	6
CGCAGAG	2125	0.0	23.912983	8
TGCGCGG	50	0.0016558748	23.745367	12-13
GTACATG	3485	0.0	23.397964	1
ACGCAGA	2185	0.0	22.821634	7
ACCGTGC	105	0.0021983106	22.614635	8
ACATGGG	3350	0.0	22.540377	3
GTCCATA	255	4.674803E-10	22.440151	1
>>END_MODULE