FastQCFastQC Report
Fri 10 Feb 2017
SRR3224543_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224543_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256637
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG4980.19404840299722953No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC4810.18742426072623977No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA3530.13754836597996392No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA3050.11884490545011046No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA2840.11066214146829957No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA2740.10676558719124678No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT2710.10559662090813093No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGTA200.002074665271.228967
CCGTGCG750.063.3516659
AAACCGT701.8189894E-1161.053396
ACCGTGC801.8189894E-1259.3574648
GGTATCA2650.055.6037981
AACCGTG902.2555469E-1047.485977
CCCTACA500.001615820837.9887732
GTATCAA5250.037.1204451
ATCAACG5000.037.0390553
TCAACGC5350.034.615944
TCTAATG2350.034.3515552
GTATTAT1351.1816155E-831.6881851
ACCCCGA300.00395464631.65731652-53
ACATGGG7600.030.6159553
CGCAGAG5900.030.5841838
TGCGCGG701.6940248E-830.55050712-13
GTGCGCG701.695662E-830.5475310-11
CATGGGA2500.030.391024
GTCTAAT2550.029.8241771
ACGCAGA6250.029.6312477
Produced by FastQC (version 0.11.5)