##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224543_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	256637
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.397261501654086	34.0	31.0	34.0	31.0	34.0
2	32.560250470508926	34.0	31.0	34.0	31.0	34.0
3	32.69860931977852	34.0	33.0	34.0	31.0	34.0
4	35.97529584588349	37.0	37.0	37.0	35.0	37.0
5	36.012605353086265	37.0	37.0	37.0	35.0	37.0
6	36.11264159104104	37.0	37.0	37.0	35.0	37.0
7	36.07050814964327	37.0	37.0	37.0	35.0	37.0
8	36.115860144873885	37.0	37.0	37.0	35.0	37.0
9	37.84842014206837	39.0	39.0	39.0	37.0	39.0
10-11	37.935030802261565	39.0	39.0	39.0	37.0	39.0
12-13	37.948551845602935	39.0	39.0	39.0	37.0	39.0
14-15	39.491513304784576	41.0	40.0	41.0	38.0	41.0
16-17	39.3426415520755	41.0	40.0	41.0	37.5	41.0
18-19	39.439038408335506	41.0	40.0	41.0	37.0	41.0
20-21	39.4980478263072	41.0	40.0	41.0	37.5	41.0
22-23	39.46641170213181	41.0	40.0	41.0	37.0	41.0
24-25	39.431905765731365	41.0	40.0	41.0	37.0	41.0
26-27	39.293998916757914	41.0	40.0	41.0	36.5	41.0
28-29	39.226756079598815	41.0	40.0	41.0	36.0	41.0
30-31	39.151560375160244	41.0	40.0	41.0	36.0	41.0
32-33	38.95963169768973	41.0	39.0	41.0	35.0	41.0
34-35	38.9846066623285	41.0	39.0	41.0	35.0	41.0
36-37	38.99199063268352	41.0	39.0	41.0	35.5	41.0
38-39	38.88969244497092	41.0	39.0	41.0	35.0	41.0
40-41	38.812036066506394	41.0	39.0	41.0	35.0	41.0
42-43	38.69127405635197	41.0	39.0	41.0	35.0	41.0
44-45	38.541652216944556	40.5	39.0	41.0	35.0	41.0
46-47	38.39313310239755	40.0	38.0	41.0	34.5	41.0
48-49	38.21991567856544	40.0	38.0	41.0	34.0	41.0
50-51	37.007937281062354	39.0	36.5	40.0	32.0	40.5
52-53	37.34206291376536	39.5	37.0	40.5	33.0	40.5
54-55	37.833665839298305	40.0	37.0	41.0	33.5	41.0
56-57	37.73430955006488	40.0	36.5	41.0	33.0	41.0
58-59	37.63848159072932	40.0	36.0	41.0	33.0	41.0
60-61	37.45365827998301	40.0	35.5	41.0	33.0	41.0
62-63	37.23929129470809	40.0	35.0	41.0	33.0	41.0
64-65	37.05780733097721	39.0	35.0	41.0	33.0	41.0
66-67	36.878039799405386	39.0	35.0	41.0	32.5	41.0
68-69	36.63999540206595	39.0	35.0	41.0	32.0	41.0
70-71	36.39980789987414	38.0	35.0	41.0	32.0	41.0
72-73	36.12355389129393	37.0	35.0	40.0	31.0	41.0
74-75	35.86158075413911	37.0	35.0	40.0	31.0	41.0
76-77	35.52190058331418	36.0	35.0	39.0	31.0	41.0
78-79	35.235529171553594	36.0	35.0	39.0	31.0	40.5
80-81	34.87879767921227	35.5	35.0	38.5	31.0	39.5
82-83	34.5441830289475	35.0	35.0	37.0	30.0	39.0
84-85	34.214852106282414	35.0	34.5	37.0	30.0	39.0
86-87	33.9082965433667	35.0	34.0	36.5	30.0	37.5
88-89	33.665751625837274	35.0	34.0	36.0	30.0	37.0
90-91	33.419136757365465	35.0	34.0	36.0	29.0	37.0
92-93	33.21123220735903	35.0	34.0	35.5	29.0	36.5
94-95	33.03023141635852	35.0	34.0	35.0	29.0	36.0
96-97	32.8784197134474	35.0	34.0	35.0	29.0	36.0
98-99	32.78997962102113	35.0	34.0	35.0	29.0	36.0
100-101	31.711023741705212	34.5	32.0	35.0	24.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	60.0
8	241.0
9	155.0
10	98.0
11	207.0
12	281.0
13	344.0
14	365.0
15	383.0
16	436.0
17	495.0
18	516.0
19	678.0
20	772.0
21	1054.0
22	1216.0
23	1166.0
24	1031.0
25	1041.0
26	1049.0
27	1203.0
28	1286.0
29	1530.0
30	1832.0
31	2364.0
32	3062.0
33	4416.0
34	7182.0
35	13038.0
36	27909.0
37	48906.0
38	80954.0
39	51354.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.4824152964089	22.237400328627686	13.798069605061336	22.482114769902076
2	14.450371536450318	21.08269657142189	41.57701344700881	22.88991844511898
3	18.22464502252217	28.330787574619304	30.950840879689522	22.493726523169002
4	11.566142060575833	17.785821997607513	42.05200341338154	28.596032528435106
5	11.418072998047826	40.576378308661646	36.42070317218484	11.584845521105686
6	27.07754532666763	40.70925080950915	19.725137061296696	12.488066802526527
7	23.68013965250529	33.49127366669654	25.571137443159014	17.257449237639154
8	23.451411916442293	38.10713186329329	21.871748812525084	16.569707407739337
9	25.054064690594107	16.869352431644696	22.36894913827702	35.70763373948417
10-11	23.497586084625365	27.118264318862828	30.3077888223444	19.0763607741674
12-13	23.45647743700246	25.146802682387964	30.654387325288248	20.74233255532133
14-15	20.946227757313533	27.317234793116217	27.369077549747605	24.367459899822645
16-17	20.797710243657058	30.43831754381518	28.28133743295385	20.482634779573907
18-19	20.450709934404635	29.82270153133806	29.41307152350403	20.313517010753273
20-21	21.46730126803332	28.343058950101153	29.472906653569243	20.716733128296283
22-23	21.387881623581706	28.769446718914494	28.834171638008343	21.008500019495457
24-25	20.935326097546717	28.829478360841016	28.759134043921353	21.476061497690914
26-27	20.930146471475275	29.451326192248196	29.01043107579967	20.60809626047686
28-29	20.83390158083207	29.35157440275564	29.274227800356144	20.54029621605614
30-31	20.765711880983645	28.75949298035747	29.20506396193846	21.269731176720427
32-33	20.777596371528656	28.960555181053394	29.3558606124604	20.90598783495755
34-35	21.082891399135743	28.62876358436235	29.27948814863017	21.008856867871742
36-37	20.805239650482108	28.37572394010492	29.66946239408844	21.149574015324536
38-39	20.77966359277307	28.645077321445623	29.32467213641775	21.250586949363562
40-41	21.144111882225953	28.812245009293648	29.33674165228133	20.706901456199077
42-43	21.1520889063806	28.54212006024249	29.143570789796186	21.162220243580727
44-45	20.967411953821426	28.592917336450874	28.95201909493887	21.487651614788835
46-47	21.20738630828758	28.366525481516696	28.90366548860842	21.5224227215873
48-49	20.94397923915881	28.709617085611193	29.049786273997903	21.29661740123209
50-51	20.86351539333767	28.90405514403613	28.810343013673005	21.42208644895319
52-53	21.04723792750071	28.81852577765482	28.562132506224746	21.572103788619724
54-55	21.260769101883202	28.422830690820106	28.44854794904866	21.86785225824803
56-57	21.090879335403702	28.72559295814711	28.508360057201415	21.67516764924777
58-59	21.39437456766789	28.492317884665972	28.657164291072768	21.456143256593368
60-61	21.21621331961503	28.90489357597582	28.6885964655883	21.19029663882085
62-63	20.958934543158485	28.567503098617898	29.114146067678494	21.359416290545123
64-65	21.505621569361278	28.230843001497007	28.441164545908183	21.822370883233532
66-67	21.076999522589322	28.435359568186914	28.1773629392909	22.31027796993287
68-69	20.93599130289085	28.608501502121676	28.995818997260724	21.459688197726752
70-71	21.574636548899807	28.00219765661226	28.749361939237133	21.6738038552508
72-73	21.163355244956886	28.658572224581803	28.914186185156467	21.263886345304847
74-75	21.08328105456345	28.504268675210508	28.76397401777608	21.648476252449957
76-77	21.19452767917331	28.05402182849706	28.73845158726138	22.012998905068248
78-79	21.22833614585079	28.02085809712733	28.399671071861444	22.35113468516043
80-81	21.075727034898456	28.509047242160424	28.418399373067643	21.996826349873484
82-83	21.089718973983114	28.273357595226635	28.633387458017634	22.00353597277262
84-85	21.19960166583193	27.996032248697716	29.108405747392997	21.695960338077356
86-87	20.733395193612274	29.05178329148667	28.779065234003397	21.43575628089765
88-89	21.24918632162806	27.948244208753813	28.37525482262786	22.42731464699027
90-91	21.08620347027124	27.96966142839887	28.547715255399652	22.396419845930243
92-93	21.668881242864323	27.707075863197637	28.83300277049327	21.791040123444763
94-95	21.455596815735845	27.552730120754216	28.844632691311073	22.147040372198866
96-97	21.39032953159521	28.09142874955677	28.83391716704918	21.684324551798845
98-99	20.798288630027294	28.453863733334895	28.66681083945581	22.08103679718201
100-101	21.249403073804444	27.8757224025183	28.432203802785335	22.44267072089193
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	2.0
2	3.5
3	3.0
4	2.5
5	4.0
6	5.5
7	7.5
8	13.0
9	19.5
10	20.0
11	17.0
12	23.5
13	25.0
14	22.0
15	31.0
16	33.5
17	40.0
18	51.5
19	56.0
20	56.5
21	62.5
22	77.5
23	102.0
24	136.5
25	192.5
26	339.5
27	571.0
28	1148.0
29	2389.0
30	4354.5
31	6387.0
32	8769.5
33	11465.0
34	13370.0
35	14413.0
36	15369.0
37	15294.0
38	13838.5
39	12231.0
40	10078.0
41	7974.0
42	6426.0
43	5370.5
44	4951.0
45	4845.0
46	4948.5
47	4810.5
48	4797.5
49	5424.5
50	6283.0
51	6444.0
52	6253.5
53	6863.5
54	7311.5
55	7044.5
56	5974.5
57	5100.0
58	4925.0
59	4664.5
60	3957.0
61	3071.0
62	2480.0
63	1725.5
64	1028.0
65	684.5
66	553.0
67	500.5
68	407.5
69	265.5
70	152.0
71	103.0
72	70.5
73	51.0
74	44.5
75	34.5
76	22.0
77	15.0
78	8.0
79	4.5
80	4.0
81	3.5
82	5.0
83	4.0
84	1.0
85	1.0
86	0.5
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	1.0
94	1.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16365527963621768
2	0.0
3	3.8965542770528025E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.035848299348885775
16-17	0.07422935897785587
18-19	0.024937947373137936
20-21	0.03818623191511746
22-23	0.0650724564267818
24-25	0.016365527963621767
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02143104852379041
38-39	0.005260348274021283
40-41	0.003896554277052802
42-43	0.0029224157077896015
44-45	0.009546557978779366
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.013443112255832168
60-61	0.01772932196059025
62-63	0.027665535367074893
64-65	0.04870692846316003
66-67	0.01733966653288497
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.017924149674442887
80-81	0.05844831415579203
82-83	0.05085003331553907
84-85	0.026691396797811694
86-87	0.05650003701726563
88-89	0.0335103667826541
90-91	0.0
92-93	0.001558621710821121
94-95	0.0
96-97	0.0
98-99	1.9482771385264012E-4
100-101	0.04461554647225459
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	256637.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.47179881436005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.05682179994923	29.58552465232146
2	14.906095118851203	12.959895362288279
3	5.555531489693511	7.245268416803949
4	3.0695943014123928	5.337631436508225
5	1.969500550684999	4.28088658520748
6	1.3307147368300607	3.470913799126828
7	0.9594944834755958	2.9197665804397595
8	0.7319457481634258	2.5455198645749357
9	0.5133439929572571	2.008438810595833
>10	2.683421775442102	20.2850944661811
>50	0.15726647966328902	4.631297484705836
>100	0.06626952287698341	4.729762541246328
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	498	0.19404840299722953	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	481	0.18742426072623977	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	353	0.13754836597996392	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	305	0.11884490545011046	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	284	0.11066214146829957	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	274	0.10676558719124678	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	271	0.10559662090813093	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	9.741385692632006E-4	0.0
24-25	0.0	0.0	0.0	0.001558621710821121	0.0
26-27	0.0	0.0	0.0	0.0023379325662316814	0.0
28-29	0.0	0.0	0.0	0.003506898849347522	0.0
30-31	0.0	0.0	0.0	0.008182763981810885	0.0
32-33	0.0	0.0	0.0	0.01987242681296929	0.0
34-35	0.0	0.0	0.0	0.039550025912085944	0.0
36-37	0.0	0.0	0.0	0.07345004812244532	0.0
38-39	0.0	0.0	0.0	0.12215697658560536	0.0
40-41	0.0	0.0	0.0	0.1743708038981129	0.0
42-43	0.0	0.0	0.0	0.22463635407209404	0.0
44-45	0.0	0.0	0.0	0.2947743310590445	0.0
46-47	0.0	0.0	0.0	0.3721209334585426	0.0
48-49	0.0	0.0	0.0	0.4582347829814095	0.0
50-51	0.0	0.0	0.0	0.5503882916337084	0.0
52-53	0.0	0.0	0.0	0.6417624894305964	0.0
54-55	0.0	0.0	0.0	0.7446315223447906	0.0
56-57	0.0	0.0	0.0	0.8741919520567962	0.0
58-59	0.0	0.0	0.0	1.024209291723329	0.0
60-61	0.0	0.0	0.0	1.1831887062270834	0.0
62-63	0.0	0.0	0.0	1.33495949531829	0.0
64-65	0.0	0.0	0.0	1.484976834984823	0.0
66-67	0.0	0.0	0.0	1.6745442005634417	0.0
68-69	0.0	0.0	0.0	1.8929460677922512	0.0
70-71	0.0	0.0	0.0	2.1154393170119663	0.0
72-73	0.0	0.0	0.0	2.3507911953459555	0.0
74-75	0.0	0.0	0.0	2.625108616450473	0.0
76-77	1.9482771385264012E-4	0.0	0.0	2.8931915507117054	0.0
78-79	3.8965542770528025E-4	0.0	0.0	3.1871865709153395	0.0
80-81	3.8965542770528025E-4	0.0	0.0	3.530083347295986	0.0
82-83	3.8965542770528025E-4	0.0	0.0	3.867914603116464	0.0
84-85	3.8965542770528025E-4	0.0	0.0	4.2380872594364805	0.0
86-87	3.8965542770528025E-4	0.0	0.0	4.658720293644331	0.0
88-89	3.8965542770528025E-4	0.0	0.0	5.107018863219255	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGTA	20	0.0020746652	71.22896	7
CCGTGCG	75	0.0	63.351665	9
AAACCGT	70	1.8189894E-11	61.05339	6
ACCGTGC	80	1.8189894E-12	59.357464	8
GGTATCA	265	0.0	55.603798	1
AACCGTG	90	2.2555469E-10	47.48597	7
CCCTACA	50	0.0016158208	37.988773	2
GTATCAA	525	0.0	37.120445	1
ATCAACG	500	0.0	37.039055	3
TCAACGC	535	0.0	34.61594	4
TCTAATG	235	0.0	34.351555	2
GTATTAT	135	1.1816155E-8	31.688185	1
ACCCCGA	30	0.003954646	31.657316	52-53
ACATGGG	760	0.0	30.615955	3
CGCAGAG	590	0.0	30.584183	8
TGCGCGG	70	1.6940248E-8	30.550507	12-13
GTGCGCG	70	1.695662E-8	30.54753	10-11
CATGGGA	250	0.0	30.39102	4
GTCTAAT	255	0.0	29.824177	1
ACGCAGA	625	0.0	29.631247	7
>>END_MODULE