FastQCFastQC Report
Fri 10 Feb 2017
SRR3224543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256637
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG3920.15274492766046985No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC3660.14261388654013257No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC3470.13521043341373223No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA3470.13521043341373223No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA3120.12157249344404743No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG3010.11728628373928936No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA2800.10910351975747846No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT2740.10676558719124678No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT2660.10364834376960454No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG505.5551936E-966.489649
ACCGTGC603.274181E-1063.323468
AAACCGT602.3446773E-855.4080286
CGTATAC352.785998E-454.2772523
GGTATCA2750.053.6940461
AACCGTG707.898416E-847.4925967
GTATCAA4750.043.1193281
GTACAAC704.301697E-640.707946
ACAACGT550.00257787934.540078
ATAGGAG702.0507828E-433.9232833
ATCAACG6000.033.244823
TCAACGC6050.032.9700664
CAACGTG753.074646E-431.6617329
AGCGTTA300.003951946731.6617314-15
ACCCTTG1051.8963765E-631.661737
TGCGCGG601.5051228E-731.6617312-13
CGGTCTA601.5051228E-731.6617328-29
CAACGCA6550.030.4532685
GCGCGGC552.7328824E-630.2225612-13
AACGCAG6700.029.7714796
Produced by FastQC (version 0.11.5)