##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224543_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	256637
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.18274839559378	34.0	33.0	34.0	31.0	34.0
2	33.31909272630213	34.0	34.0	34.0	31.0	34.0
3	33.437271320970865	34.0	34.0	34.0	31.0	34.0
4	36.64384714596882	37.0	37.0	37.0	35.0	37.0
5	36.683054275104524	37.0	37.0	37.0	35.0	37.0
6	36.726789200310165	37.0	37.0	37.0	37.0	37.0
7	36.70259159824967	37.0	37.0	37.0	36.0	37.0
8	36.69806769873401	37.0	37.0	37.0	36.0	37.0
9	38.550376601970875	39.0	39.0	39.0	38.0	39.0
10-11	38.580206673238855	39.0	39.0	39.0	37.5	39.0
12-13	38.578614930816684	39.0	39.0	39.0	38.0	39.0
14-15	40.206614790540726	41.0	40.0	41.0	39.0	41.0
16-17	40.22109828278853	41.0	40.0	41.0	39.0	41.0
18-19	40.2259767687434	41.0	40.0	41.0	39.0	41.0
20-21	40.23163456555368	41.0	40.0	41.0	39.0	41.0
22-23	40.16847336899979	41.0	40.0	41.0	39.0	41.0
24-25	40.14261583481727	41.0	40.0	41.0	38.0	41.0
26-27	40.095317510725266	41.0	40.0	41.0	38.0	41.0
28-29	40.061359040200756	41.0	40.0	41.0	38.0	41.0
30-31	40.002339880843365	41.0	40.0	41.0	38.0	41.0
32-33	39.95494803944871	41.0	40.0	41.0	38.0	41.0
34-35	39.88648947735518	41.0	40.0	41.0	38.0	41.0
36-37	39.79335403702506	41.0	40.0	41.0	38.0	41.0
38-39	39.70108168346731	41.0	40.0	41.0	37.0	41.0
40-41	39.58274527835036	41.0	40.0	41.0	37.0	41.0
42-43	39.50294384675631	41.0	40.0	41.0	36.5	41.0
44-45	39.30275057766417	41.0	39.0	41.0	36.0	41.0
46-47	39.10539010353145	41.0	39.0	41.0	35.0	41.0
48-49	39.020049720032574	41.0	39.0	41.0	35.0	41.0
50-51	38.90834135374089	41.0	39.0	41.0	35.0	41.0
52-53	38.724725974820466	40.0	38.0	41.0	35.0	41.0
54-55	38.57619517061063	40.0	37.5	41.0	35.0	41.0
56-57	38.39948058931487	40.0	37.0	41.0	35.0	41.0
58-59	38.20152394237776	40.0	36.5	41.0	35.0	41.0
60-61	38.02327801525111	40.0	36.0	41.0	34.5	41.0
62-63	37.85530535347593	40.0	35.0	41.0	34.0	41.0
64-65	37.67021512876163	39.5	35.0	41.0	34.0	41.0
66-67	37.44440980840643	39.0	35.0	41.0	34.0	41.0
68-69	37.25113876798747	39.0	35.0	41.0	34.0	41.0
70-71	37.007056659795744	38.5	35.0	41.0	34.0	41.0
72-73	36.753765045570205	37.5	35.0	40.0	33.0	41.0
74-75	36.46423352829093	37.0	35.0	40.0	33.0	41.0
76-77	34.50542400355366	35.0	33.0	38.0	30.0	39.0
78-79	35.81981358884339	36.0	35.0	39.0	33.0	40.0
80-81	35.694917334601016	36.0	35.0	39.0	33.0	39.5
82-83	35.39953514107475	35.5	35.0	37.0	33.0	39.0
84-85	35.08533453866745	35.0	35.0	37.0	33.0	39.0
86-87	34.79061281109115	35.0	35.0	37.0	33.0	38.0
88-89	34.54808737633311	35.0	35.0	36.0	33.0	37.0
90-91	34.33098111340142	35.0	35.0	36.0	33.0	37.0
92-93	34.17398894157896	35.0	35.0	36.0	33.0	36.5
94-95	34.01949056449382	35.0	35.0	35.0	32.0	36.0
96-97	33.934929491850355	35.0	35.0	35.0	32.0	36.0
98-99	33.85634573346789	35.0	35.0	35.0	32.0	36.0
100-101	33.093121802390144	34.5	33.5	35.0	29.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	11.0
16	26.0
17	66.0
18	151.0
19	223.0
20	318.0
21	503.0
22	716.0
23	696.0
24	540.0
25	516.0
26	655.0
27	887.0
28	1135.0
29	1328.0
30	1398.0
31	1642.0
32	2074.0
33	2762.0
34	4669.0
35	9459.0
36	26166.0
37	53009.0
38	87163.0
39	60506.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.80104211931523	22.24114374747985	13.990706268766564	21.96710786443836
2	14.40127495255945	21.34220708627361	41.79249289852983	22.46402506263711
3	17.76478060451143	28.955294832779373	31.156068688458795	22.1238558742504
4	11.425476451174227	17.861804806010046	42.631421034379294	28.08129770843643
5	11.441452323710143	40.01566414819375	37.132993294030086	11.409890234066015
6	26.863234841429723	40.783285340773155	19.820602641084488	12.532877176712635
7	23.612729263512275	33.28865284428979	25.918710084672124	17.179907807525804
8	23.27139110884245	37.912693804868354	22.014362699065217	16.80155238722398
9	25.00769569469718	16.673745406936646	22.80224597388529	35.51631292448088
10-11	23.69631035275506	27.07540222181525	30.42020441323737	18.808083012192316
12-13	23.566944750756903	25.045492271184592	30.895194379610107	20.492368598448394
14-15	21.080748294283367	27.576109446416535	27.452783503547813	23.89035875575229
16-17	20.358521959031627	30.759009807627113	28.395359983166884	20.487108250174373
18-19	20.502889294996436	29.533348659780156	29.982231712496638	19.98153033272677
20-21	21.14544225347722	28.418626490202993	29.925243877966523	20.51068737835326
22-23	21.570396232701228	28.616156755166493	29.16480763123491	20.648639380897365
24-25	21.18194641437678	28.761942985395656	28.937678268052807	21.118432332174752
26-27	20.75841147807044	29.57894388191491	29.06438684713506	20.59825779287959
28-29	21.069182389937108	29.229890969738065	29.262233756517265	20.438692883807562
30-31	20.77982804271461	28.50816436669414	29.571743361642188	21.140264228949064
32-33	20.66619518268056	29.123877546646714	29.81025691980681	20.399670350865915
34-35	21.277325332190316	28.637727467560303	29.31204457779683	20.77290262245256
36-37	20.98117912948603	28.561937419631377	29.187546272844173	21.269337178038423
38-39	20.672389406048232	28.773715403468714	29.212272587351006	21.341622603132052
40-41	20.86858091389784	28.757934358646647	29.765778122406356	20.607706605049156
42-43	20.990543062769593	28.680782583961	29.391319256381582	20.93735509688782
44-45	21.00101711163677	28.609479792213055	29.0687388205402	21.320764275609974
46-47	21.261382470507943	28.24120113093497	29.03110071170908	21.466315686848006
48-49	20.819808832067583	28.247536365159586	29.598297958563396	21.334356844209434
50-51	21.028924122398564	28.640063591765802	28.834306822476886	21.49670546335875
52-53	21.082112088280333	28.922758604565985	28.75871366950206	21.236415637651625
54-55	21.14893799413179	28.578498034188364	28.697148111924626	21.575415859755218
56-57	21.32001652261338	28.95061920831742	28.24139382272483	21.48797044634437
58-59	21.555549925060273	28.35742643920332	28.545701539139195	21.54132209659721
60-61	21.312587210888594	28.876450916347707	28.68273555709731	21.12822631566639
62-63	21.197839750308802	28.68584810452117	28.755206770652713	21.361105374517315
64-65	21.313957067764974	28.220404696127215	28.730853306421132	21.73478492968668
66-67	21.141729368719243	28.476603139843437	28.40821861228116	21.973448879156162
68-69	21.14835351099023	28.23189953124452	29.002053484104007	21.61769347366124
70-71	21.470598549702498	28.301647852803764	28.782287822878228	21.445465774615506
72-73	21.189072503185432	28.867622361545685	28.35639444039636	21.586910694872525
74-75	21.14387247357162	28.20657192844368	28.984908645284975	21.66464695269973
76-77	21.414028846359916	27.77304776618979	28.818889046916734	21.99403434053356
78-79	21.270049724876465	27.86288248406154	28.70949137218836	22.15757641887363
80-81	21.204073719753488	28.338928495580053	28.52772868886157	21.929269095804894
82-83	21.034502241505947	28.428257197464884	28.68835371567846	21.8488868453507
84-85	21.340562909391306	27.89238244795056	29.03156672420713	21.735487918451003
86-87	20.53932207748688	29.052708689705693	28.809758530531447	21.598210702275978
88-89	21.397343329293903	27.67469226962597	28.27456680057825	22.653397600501876
90-91	21.23700012079318	28.052852862213946	28.332820287020187	22.377326729972687
92-93	21.567038268059555	27.884132062017557	28.725008475005552	21.823821194917333
94-95	21.155951791830486	27.463304200095855	28.89158617034956	22.4891578377241
96-97	21.34668812369222	27.967518323546486	28.865479256693305	21.820314296067984
98-99	20.32812883567062	28.35678409582406	28.804303354543574	22.510783713961743
100-101	20.81255482990545	28.223413588069015	28.447412028462814	22.516619553562727
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	2.0
4	2.0
5	1.0
6	4.5
7	8.0
8	10.5
9	13.5
10	15.5
11	18.5
12	22.5
13	24.5
14	18.5
15	22.5
16	32.0
17	30.5
18	31.5
19	43.0
20	56.0
21	63.5
22	79.0
23	106.0
24	137.5
25	193.5
26	355.0
27	671.5
28	1263.5
29	2466.5
30	4324.0
31	6621.0
32	9137.0
33	11796.0
34	13664.0
35	14444.5
36	15341.5
37	15329.5
38	13727.0
39	11953.5
40	9916.5
41	7754.0
42	6380.0
43	5533.0
44	4885.5
45	4621.0
46	4602.5
47	4689.0
48	4920.0
49	5514.5
50	6383.5
51	6488.0
52	6179.5
53	6704.5
54	7326.5
55	7243.0
56	6094.5
57	5043.5
58	4879.0
59	4744.0
60	4037.0
61	2985.5
62	2427.5
63	1728.0
64	966.5
65	616.5
66	495.5
67	440.0
68	354.5
69	227.0
70	117.0
71	71.0
72	57.0
73	59.5
74	39.5
75	29.5
76	25.0
77	12.0
78	5.0
79	1.5
80	2.5
81	3.0
82	1.5
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.46602789153551516
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	9.741385692632005E-4
22-23	0.0029224157077896015
24-25	3.8965542770528025E-4
26-27	0.0033120711354948816
28-29	0.004286209704758083
30-31	0.0013637939969684807
32-33	1.9482771385264012E-4
34-35	0.0027275879939369614
36-37	0.0027275879939369614
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.010910351975747846
46-47	0.0826069506735194
48-49	0.001558621710821121
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.007403453126400324
58-59	0.037991404201264824
60-61	0.029224157077896017
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	5.844831415579203E-4
78-79	0.009741385692632006
80-81	0.006429314557137123
82-83	5.844831415579203E-4
84-85	0.0023379325662316814
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0631241792882554
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	256637.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.32666657109354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.80915824145383	28.27808964781442
2	15.465329079399378	13.091916547119492
3	5.921780867114785	7.519477328083461
4	3.1621863519364113	5.3537922941630045
5	1.9697122097507311	4.168567597156553
6	1.3378348621264973	3.3975654081847884
7	1.0003260768853957	2.963832782308875
8	0.6629042642257539	2.244682220835166
9	0.5835126162110862	2.222832955175381
>10	2.8471024921615364	21.071530852847317
>50	0.1746566827160981	4.994229103167023
>100	0.0654962560185368	4.693483263144517
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	392	0.15274492766046985	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	366	0.14261388654013257	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	347	0.13521043341373223	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	347	0.13521043341373223	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	312	0.12157249344404743	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	301	0.11728628373928936	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	280	0.10910351975747846	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	274	0.10676558719124678	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	266	0.10364834376960454	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.008572419409516165	0.0
14-15	0.0	0.0	0.0	0.008572419409516165	0.0
16-17	0.0	0.0	0.0	0.008572419409516165	0.0
18-19	0.0	0.0	0.0	0.008572419409516165	0.0
20-21	0.0	0.0	0.0	0.008767247123368805	0.0
22-23	0.0	0.0	0.0	0.009936213406484646	0.0
24-25	0.0	0.0	0.0	0.010520696548042566	0.0
26-27	0.0	0.0	0.0	0.011300007403453126	0.0
28-29	0.0	0.0	0.0	0.012468973686568966	0.0
30-31	0.0	0.0	0.0	0.01714483881903233	0.0
32-33	0.0	0.0	0.0	0.028834501650190737	0.0
34-35	0.0	0.0	0.0	0.048122445321602106	0.0
36-37	0.0	0.0	0.0	0.08221729524581413	0.0
38-39	0.0	0.0	0.0	0.13131387913667944	0.0
40-41	0.0	0.0	0.0	0.1841121895907449	0.0
42-43	0.0	0.0	0.0	0.23515705062013662	0.0
44-45	0.0	0.0	0.0	0.3060743384624976	0.0
46-47	0.0	0.0	0.0	0.38536921800052215	0.0
48-49	0.0	0.0	0.0	0.47343134466191544	0.0
50-51	0.0	0.0	0.0	0.5675331304527407	0.0
52-53	0.0	0.0	0.0	0.6602711222465973	0.0
54-55	0.0	0.0	0.0	0.7629453274469387	0.0
56-57	0.0	0.0	0.0	0.8930902403005023	0.0
58-59	0.0	0.0	0.0	1.0438868908224457	0.0
60-61	0.0	0.0	0.0	1.2032559607539053	0.0
62-63	0.0	0.0	0.0	1.3561957161282279	0.0
64-65	0.0	0.0	0.0	1.5073820220778766	0.0
66-67	0.0	0.0	0.0	1.6979235262257584	1.9482771385264012E-4
68-69	0.0	0.0	0.0	1.9200271200177683	3.8965542770528025E-4
70-71	0.0	0.0	0.0	2.1464169235145363	3.8965542770528025E-4
72-73	0.0	0.0	0.0	2.385275700697873	5.844831415579203E-4
74-75	0.0	0.0	0.0	2.6619310543686217	7.793108554105605E-4
76-77	0.0	0.0	0.0	2.930988127199118	7.793108554105605E-4
78-79	0.0	0.0	0.0	3.227905563110541	7.793108554105605E-4
80-81	0.0	0.0	0.0	3.5717764780604515	7.793108554105605E-4
82-83	0.0	0.0	0.0	3.912335321874866	7.793108554105605E-4
84-85	0.0	0.0	0.0	4.284845910761113	7.793108554105605E-4
86-87	0.0	0.0	0.0	4.708011705249048	7.793108554105605E-4
88-89	0.0	0.0	0.0	5.157868896534794	7.793108554105605E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	50	5.5551936E-9	66.48964	9
ACCGTGC	60	3.274181E-10	63.32346	8
AAACCGT	60	2.3446773E-8	55.408028	6
CGTATAC	35	2.785998E-4	54.277252	3
GGTATCA	275	0.0	53.694046	1
AACCGTG	70	7.898416E-8	47.492596	7
GTATCAA	475	0.0	43.119328	1
GTACAAC	70	4.301697E-6	40.70794	6
ACAACGT	55	0.002577879	34.54007	8
ATAGGAG	70	2.0507828E-4	33.923283	3
ATCAACG	600	0.0	33.24482	3
TCAACGC	605	0.0	32.970066	4
CAACGTG	75	3.074646E-4	31.661732	9
AGCGTTA	30	0.0039519467	31.66173	14-15
ACCCTTG	105	1.8963765E-6	31.66173	7
TGCGCGG	60	1.5051228E-7	31.66173	12-13
CGGTCTA	60	1.5051228E-7	31.66173	28-29
CAACGCA	655	0.0	30.453268	5
GCGCGGC	55	2.7328824E-6	30.22256	12-13
AACGCAG	670	0.0	29.771479	6
>>END_MODULE