FastQCFastQC Report
Fri 10 Feb 2017
SRR3224542_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224542_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227741
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG7030.30868398751213No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA5770.25335798121550357No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA4120.18090725868420704No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC3920.1721253529228378No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA3740.16422163773760545No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT3450.15148787438362No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT3100.13611953930122375No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA2850.12514215709951215No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA2810.1233857759472383No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC2640.11592115605007441No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG2630.11548206076200597No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA2420.10626105971256823No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA2410.10582196442449976No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC2290.10055282096767819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGT900.058.0334136
GTATCAA3700.048.808051
CCGTGCG1001.2732926E-1147.523639
AACCGTG1201.8189894E-1243.5250637
ACCGTGC1201.8189894E-1243.5250638
GGTATCA1900.040.0198941
ATCAACG4700.038.389613
TCAACGC4700.037.3793564
ATCTTAT651.3209681E-436.5566331
GTATTAA1255.5824785E-934.217011
GTACATG9450.034.1968961
CGGAAAC352.0454063E-433.94544620-21
AATGCCG702.0422031E-433.9454469
TCTACAC1001.2969958E-633.237323
CAGGAGT1001.2969958E-633.237324
ACATGGG9500.031.9877973
AGACGAG300.00395159831.66154352-53
TACATGG10050.031.654592
CACCGTA600.00395173131.654597
ACCGCAA2100.031.6545898
Produced by FastQC (version 0.11.5)