##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224542_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	227741
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.088539173886126	34.0	31.0	34.0	30.0	34.0
2	32.252176814890596	34.0	31.0	34.0	31.0	34.0
3	32.403893018824014	34.0	31.0	34.0	31.0	34.0
4	35.691026209597744	37.0	35.0	37.0	35.0	37.0
5	35.701160528846366	37.0	37.0	37.0	35.0	37.0
6	35.8110660794499	37.0	37.0	37.0	35.0	37.0
7	35.76576461857988	37.0	37.0	37.0	35.0	37.0
8	35.802538848955614	37.0	37.0	37.0	35.0	37.0
9	37.508893875059826	39.0	38.0	39.0	35.0	39.0
10-11	37.58931856802245	39.0	38.5	39.0	35.0	39.0
12-13	37.58479808203178	39.0	38.5	39.0	35.0	39.0
14-15	39.05601977685177	41.0	40.0	41.0	36.0	41.0
16-17	38.88919649953236	41.0	39.5	41.0	35.5	41.0
18-19	38.984820475891475	41.0	39.5	41.0	36.0	41.0
20-21	39.04868249458815	41.0	40.0	41.0	36.0	41.0
22-23	39.00683451815878	41.0	39.5	41.0	36.0	41.0
24-25	38.945611462143404	41.0	39.0	41.0	35.5	41.0
26-27	38.7706473581832	41.0	39.0	41.0	35.0	41.0
28-29	38.68836529215205	41.0	39.0	41.0	35.0	41.0
30-31	38.60039255118753	41.0	39.0	41.0	35.0	41.0
32-33	38.38202387800177	40.0	38.5	41.0	34.0	41.0
34-35	38.38668706996105	40.0	38.0	41.0	34.0	41.0
36-37	38.37235280428206	40.0	38.0	41.0	34.0	41.0
38-39	38.24081741978827	40.0	38.0	41.0	34.0	41.0
40-41	38.140490732893944	40.0	38.0	41.0	33.5	41.0
42-43	37.9878260831383	40.0	38.0	41.0	33.0	41.0
44-45	37.78905642813547	40.0	37.5	41.0	33.0	41.0
46-47	37.574597898489955	40.0	37.0	41.0	32.5	41.0
48-49	37.33779161415819	40.0	36.5	41.0	32.0	41.0
50-51	36.064828028330425	38.5	35.0	40.0	29.5	40.5
52-53	36.411840643538056	39.0	35.0	40.0	30.5	40.5
54-55	36.85958610878147	39.0	35.0	41.0	31.0	41.0
56-57	36.70962189504745	39.0	35.0	41.0	31.0	41.0
58-59	36.54494140273381	39.0	35.0	41.0	31.0	41.0
60-61	36.30299111710232	38.5	35.0	41.0	31.0	41.0
62-63	36.01749794722953	37.5	35.0	41.0	30.0	41.0
64-65	35.77879257577687	37.0	35.0	40.0	30.0	41.0
66-67	35.52729416310633	36.5	35.0	40.0	29.5	41.0
68-69	35.232483391220725	36.0	35.0	40.0	29.0	41.0
70-71	34.924837864064884	35.5	35.0	39.0	29.0	41.0
72-73	34.625666875968754	35.0	34.0	39.0	29.0	41.0
74-75	34.34384673818066	35.0	34.0	38.5	28.5	40.5
76-77	34.02736441835243	35.0	34.0	37.0	28.0	39.5
78-79	33.78192991161012	35.0	34.0	37.0	28.0	39.0
80-81	33.48079616757633	35.0	34.0	36.5	27.0	39.0
82-83	33.21622149722711	35.0	34.0	36.0	27.0	37.5
84-85	32.98968345620683	35.0	34.0	36.0	27.0	37.0
86-87	32.767997418119705	35.0	33.0	35.0	26.0	37.0
88-89	32.60844555877071	35.0	33.0	35.0	26.0	36.0
90-91	32.424890555499445	35.0	33.0	35.0	25.5	36.0
92-93	32.284123192574015	35.0	33.0	35.0	25.0	36.0
94-95	32.144925595303434	35.0	33.0	35.0	25.0	35.5
96-97	32.01232760021252	35.0	33.0	35.0	25.0	35.0
98-99	31.949504041872125	35.0	33.0	35.0	25.0	35.0
100-101	30.742617710469347	34.0	31.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	84.0
8	232.0
9	156.0
10	137.0
11	275.0
12	464.0
13	510.0
14	518.0
15	541.0
16	560.0
17	593.0
18	729.0
19	748.0
20	988.0
21	1315.0
22	1485.0
23	1429.0
24	1289.0
25	1259.0
26	1372.0
27	1419.0
28	1646.0
29	1925.0
30	2379.0
31	2933.0
32	3895.0
33	5399.0
34	8573.0
35	15847.0
36	33560.0
37	54023.0
38	61119.0
39	20334.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.27283521702801	18.926953691894983	12.723954439509214	24.07625665156779
2	16.075717591474525	19.141041797480472	39.31044475961728	25.47279585142772
3	20.597520868003567	24.928317694222823	27.929533988170775	26.544627449602835
4	12.948041854562858	15.786792891925478	38.070439666111945	33.194725587399724
5	13.518865729051862	38.840612801384026	33.931527480778605	13.708993988785506
6	30.800778076850456	36.78213409091907	18.11355882340027	14.303529008830207
7	27.704717200679717	31.091459157551782	21.783517241076485	19.420306400692013
8	26.484032299849392	34.907197210866734	19.971370987217938	18.637399502065943
9	26.496766063203374	15.560658818570216	19.94985531810258	37.99271980012382
10-11	26.458784320785455	25.184749342454804	26.979551332434653	21.376915004325088
12-13	26.54418835431477	22.587500713529842	27.752798134723218	23.11551279743217
14-15	23.475795239653593	24.814464213941044	24.678510793910867	27.03122975249449
16-17	23.280074527174296	27.657667158827255	26.11549075441187	22.946767559586583
18-19	23.261329796111983	26.74481934696101	26.902500944332687	23.09134991259432
20-21	24.337609794347692	25.34138107506759	27.00342424382197	23.317584886762738
22-23	24.078386572345007	26.039149347510875	26.385386001142408	23.497078079001714
24-25	23.264674235270867	26.143086145940842	26.735764948725265	23.856474670063026
26-27	23.274465291712954	26.958694306251402	26.98877233348409	22.77806806855156
28-29	23.0900452707242	27.041902863340372	26.9997497156858	22.868302150249626
30-31	23.032743335631263	26.102458494517894	27.31238556079055	23.55241260906029
32-33	23.106291796382735	26.341545878871177	27.149481208917148	23.402681115828948
34-35	23.76493472848543	25.96436302642036	26.723558779490737	23.54714346560347
36-37	23.241269206866487	25.76229052794315	27.42818493052917	23.56825533466119
38-39	23.2696973356314	26.190073662601137	26.708236818126927	23.831992183640534
40-41	23.73196559878274	25.87073415464739	27.23598031832324	23.16131992824663
42-43	23.5712184218962	26.205050740134105	27.047938558549856	23.175792279419838
44-45	23.621647852407794	25.857620598264887	26.790831260141807	23.729900289185515
46-47	23.676237480295597	25.91320842536039	26.48776460979797	23.92278948454604
48-49	23.31991165402804	26.143952999240366	26.695895776342425	23.840239570389173
50-51	23.27775850637347	26.425632626536284	26.40828836265758	23.888320504432667
52-53	23.62332649808335	26.45263698675249	25.886204065144174	24.037832450019977
54-55	23.668772860398434	25.856565133199556	26.197522624384717	24.27713938201729
56-57	23.69539014799739	26.258394971469723	26.01557474406177	24.030640136471114
58-59	23.772214329161844	26.40027404900822	26.462418175070763	23.36509344675917
60-61	23.67666919200237	26.61725792461324	26.094184042074286	23.6118888413101
62-63	23.49958272938903	26.170334255721002	26.6969736899899	23.633109324900076
64-65	24.341410160943475	25.539205672450265	26.013886783832284	24.105497382773976
66-67	23.309690594874724	26.046465666791104	25.830167548694526	24.81367618963965
68-69	23.45778757448154	26.216184174127626	26.872412082145946	23.45361616924489
70-71	24.015219042684453	25.643384370842316	26.453954272616702	23.88744231385653
72-73	23.47601002893638	26.27590113330494	26.624323244387266	23.623765593371417
74-75	23.567561396498654	25.986317790823787	26.556702570024722	23.889418242652837
76-77	23.732002581880295	25.494970163475177	26.378868978356994	24.394158276287538
78-79	23.540946600555998	25.010869612953947	26.782506730376504	24.66567705611355
80-81	23.536919710723105	26.123012978796318	26.009876890361245	24.33019042011933
82-83	23.321068875001373	26.36250810003405	26.25948665004558	24.056936374919
84-85	23.40116662127854	25.730016778965677	26.855349503219628	24.013467096536154
86-87	23.054380253162332	26.93551434308285	26.131915061884612	23.878190341870205
88-89	23.531698446374214	25.916823698393664	25.937468428936008	24.614009426296114
90-91	23.211674665519162	25.65282491953579	26.34659547468396	24.788904940261087
92-93	23.208098957990277	25.996012874726105	26.64260339959514	24.153284767688476
94-95	23.511576747269924	25.76391602741711	26.50379158781247	24.220715637500494
96-97	23.74385815465814	26.073478205505378	26.54835975955142	23.63430388028506
98-99	22.75526535917291	26.36269229840365	26.583340102793336	24.298702239630103
100-101	23.42718322914516	25.704533174012557	26.2027034744523	24.665580122389986
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.0
4	3.0
5	2.5
6	6.0
7	6.5
8	7.0
9	15.5
10	18.0
11	15.5
12	17.5
13	21.0
14	24.5
15	25.5
16	34.0
17	38.0
18	39.5
19	43.0
20	42.0
21	56.0
22	73.5
23	90.5
24	113.5
25	149.0
26	205.0
27	277.5
28	367.0
29	501.0
30	678.0
31	1024.0
32	1702.5
33	2617.0
34	3812.0
35	5239.5
36	6910.0
37	8311.0
38	8836.0
39	8816.5
40	8290.5
41	7511.0
42	6897.0
43	6462.0
44	6177.0
45	6240.0
46	6647.0
47	6877.5
48	7244.0
49	7956.0
50	8742.5
51	8923.0
52	8844.0
53	9402.5
54	10023.5
55	9968.5
56	8641.0
57	7413.0
58	6951.5
59	6505.5
60	5515.5
61	4253.0
62	3499.0
63	2581.0
64	1598.0
65	1041.0
66	805.5
67	705.0
68	570.0
69	403.0
70	248.5
71	156.0
72	121.0
73	94.5
74	71.5
75	64.0
76	46.5
77	27.0
78	19.5
79	11.5
80	9.0
81	7.5
82	4.5
83	3.5
84	2.5
85	2.5
86	2.0
87	2.0
88	1.5
89	0.0
90	2.0
91	3.0
92	2.0
93	1.0
94	1.0
95	2.5
96	2.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15412244611203077
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0392990282821275
16-17	0.07596348483584422
18-19	0.029419384300587072
20-21	0.04368998116281214
22-23	0.06630338849833803
24-25	0.02019838325114933
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.024369788487799738
38-39	0.005927786388924261
40-41	0.007245072253129651
42-43	0.0052691434568215645
44-45	0.01427059686222507
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	2.1954764403423187E-4
58-59	0.019539740319046636
60-61	0.02129612147132049
62-63	0.031175765452860927
64-65	0.050276410483839094
66-67	0.02019838325114933
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.018442002098875476
80-81	0.061034245041516465
82-83	0.052032791636112956
84-85	0.03293214660513478
86-87	0.06147334032958492
88-89	0.0351276230454771
90-91	0.0
92-93	0.00351276230454771
94-95	0.0
96-97	0.0
98-99	0.0010977382201711593
100-101	0.04742229111139409
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	227741.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.43683614011526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.72280147003781	39.92950098084775
2	15.163328817617536	16.205606347314323
3	4.3914651747057105	7.039980148673151
4	1.817778283255621	3.885452810455626
5	0.9650732299671428	2.5785230026482995
6	0.5801722707494559	1.8601542339046355
7	0.39596925523366494	1.4811540945911381
8	0.2652203281371739	1.133802817255501
9	0.22885587786875342	1.1006400640837328
>10	1.2512927060846688	12.583119643633008
>50	0.14830824566537032	5.389083926856881
>100	0.06776999305238778	6.141183388016397
>500	0.001964347624706892	0.6717985417195956
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	703	0.30868398751213	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	577	0.25335798121550357	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	412	0.18090725868420704	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	392	0.1721253529228378	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	374	0.16422163773760545	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	345	0.15148787438362	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	310	0.13611953930122375	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	285	0.12514215709951215	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	281	0.1233857759472383	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	264	0.11592115605007441	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	263	0.11548206076200597	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	242	0.10626105971256823	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	241	0.10582196442449976	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	229	0.10055282096767819	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	2.1954764403423187E-4	0.0
22-23	0.0	0.0	0.0	4.3909528806846375E-4	0.0
24-25	0.0	0.0	0.0	8.781905761369275E-4	0.0
26-27	0.0	0.0	0.0	0.0013172858642053911	0.0
28-29	0.0	0.0	0.0	0.0021954764403423186	0.0
30-31	0.0	0.0	0.0	0.004390952880684637	0.0
32-33	0.0	0.0	0.0	0.012514215709951216	0.0
34-35	0.0	0.0	0.0	0.023052502623594345	0.0
36-37	0.0	0.0	0.0	0.03907948063809327	0.0
38-39	0.0	0.0	0.0	0.0627906261937903	0.0
40-41	0.0	0.0	0.0	0.09484458222278816	0.0
42-43	0.0	0.0	0.0	0.12821582411599142	0.0
44-45	0.0	0.0	0.0	0.16553892360181083	0.0
46-47	0.0	0.0	0.0	0.20681388068024642	0.0
48-49	0.0	0.0	0.0	0.2590662199603936	0.0
50-51	0.0	0.0	0.0	0.31153810688457506	0.0
52-53	0.0	0.0	0.0	0.36752275611330415	0.0
54-55	0.0	0.0	0.0	0.4228487624099306	0.0
56-57	0.0	0.0	0.0	0.5007881760420829	0.0
58-59	0.0	0.0	0.0	0.5802644231824748	0.0
60-61	0.0	0.0	0.0	0.6672052902200307	0.0
62-63	0.0	0.0	0.0	0.7510724902411072	0.0
64-65	0.0	0.0	0.0	0.8448193342437242	0.0
66-67	0.0	0.0	0.0	0.9574472756332852	0.0
68-69	0.0	0.0	0.0	1.0876390285455848	0.0
70-71	0.0	0.0	0.0	1.2151962097294735	0.0
72-73	0.0	0.0	0.0	1.3484616296582521	0.0
74-75	0.0	0.0	0.0	1.5069750286509676	0.0
76-77	0.0	0.0	0.0	1.668342547016128	0.0
78-79	0.0	0.0	0.0	1.867252712511142	0.0
80-81	0.0	0.0	0.0	2.070334283242807	0.0
82-83	0.0	0.0	0.0	2.2795631880074296	0.0
84-85	0.0	0.0	0.0	2.496695807957285	0.0
86-87	0.0	0.0	0.0	2.7267817389051596	0.0
88-89	0.0	0.0	0.0	2.9900193641022037	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACCGT	90	0.0	58.033413	6
GTATCAA	370	0.0	48.80805	1
CCGTGCG	100	1.2732926E-11	47.52363	9
AACCGTG	120	1.8189894E-12	43.525063	7
ACCGTGC	120	1.8189894E-12	43.525063	8
GGTATCA	190	0.0	40.019894	1
ATCAACG	470	0.0	38.38961	3
TCAACGC	470	0.0	37.379356	4
ATCTTAT	65	1.3209681E-4	36.556633	1
GTATTAA	125	5.5824785E-9	34.21701	1
GTACATG	945	0.0	34.196896	1
CGGAAAC	35	2.0454063E-4	33.945446	20-21
AATGCCG	70	2.0422031E-4	33.945446	9
TCTACAC	100	1.2969958E-6	33.23732	3
CAGGAGT	100	1.2969958E-6	33.23732	4
ACATGGG	950	0.0	31.987797	3
AGACGAG	30	0.003951598	31.661543	52-53
TACATGG	1005	0.0	31.65459	2
CACCGTA	60	0.003951731	31.65459	7
ACCGCAA	210	0.0	31.654589	8
>>END_MODULE