FastQCFastQC Report
Fri 10 Feb 2017
SRR3224542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227741
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG5230.2296468356598065No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA3850.16905168590635855No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT3630.15939158956885233No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC3590.1576352084165785No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA3520.15456154140009923No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT3260.14314506391031917No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA3170.13919320631770302No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT3080.13524134872508684No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC2910.12777672882792296No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG2860.12558125238758064No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG2690.11811663249041673No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT2500.10977382201711593No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG2500.10977382201711593No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG2490.10933472672904747No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG2480.10889563144097902No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA2410.10582196442449976No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG2400.1053828691364313No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.10055282096767819No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTG250.005019976356.9889349
TGTACGC250.005019976356.9889342
GTACGCT250.005019976356.9889343
GGTATCA2800.054.5145841
GTACTAG459.411797E-442.4002341
CTACACT802.255365E-741.5544324
GTATCAA4600.038.3676031
AAACCGT756.9070775E-637.9926226
ACCGTGC905.6798854E-736.937278
GTCTAAT1301.9463187E-1036.6925131
CCGTGCG801.0752503E-535.6180849
AACCGTG958.672414E-734.9932067
TATACCG550.002577755134.5387465
ATAATAC851.628647E-533.5229033
GTACATG9700.032.4558531
TCGACGC300.00393525631.68835358-59
TTCGGCG300.00395222431.66051932-33
CCGCATT300.00395222431.66051966-67
GTTATTA1701.07320375E-1030.7293288
GTATAGT953.380142E-530.1264821
Produced by FastQC (version 0.11.5)