##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224542_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	227741
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.141090097962156	34.0	33.0	34.0	31.0	34.0
2	33.268054500507155	34.0	33.0	34.0	31.0	34.0
3	33.38820414418133	34.0	34.0	34.0	31.0	34.0
4	36.60793620823655	37.0	37.0	37.0	35.0	37.0
5	36.641970484014735	37.0	37.0	37.0	35.0	37.0
6	36.68255606149091	37.0	37.0	37.0	36.0	37.0
7	36.66463658278483	37.0	37.0	37.0	35.0	37.0
8	36.64929020246684	37.0	37.0	37.0	35.0	37.0
9	38.49004351434305	39.0	39.0	39.0	37.0	39.0
10-11	38.51517293767921	39.0	39.0	39.0	37.5	39.0
12-13	38.502546752670796	39.0	39.0	39.0	37.0	39.0
14-15	40.08959300257749	41.0	40.0	41.0	38.0	41.0
16-17	40.10156054465379	41.0	40.0	41.0	38.0	41.0
18-19	40.09599940283041	41.0	40.0	41.0	38.0	41.0
20-21	40.097413289657986	41.0	40.0	41.0	38.0	41.0
22-23	40.02249924256063	41.0	40.0	41.0	38.0	41.0
24-25	39.9878260831383	41.0	40.0	41.0	38.0	41.0
26-27	39.9198212003987	41.0	40.0	41.0	38.0	41.0
28-29	39.883529974839846	41.0	40.0	41.0	38.0	41.0
30-31	39.81216162219363	41.0	40.0	41.0	37.5	41.0
32-33	39.74636099780013	41.0	40.0	41.0	37.0	41.0
34-35	39.657705463662666	41.0	40.0	41.0	37.0	41.0
36-37	39.545940344514165	41.0	40.0	41.0	36.5	41.0
38-39	39.42757342770955	41.0	39.5	41.0	36.0	41.0
40-41	39.28374337514984	41.0	39.0	41.0	35.5	41.0
42-43	39.17845930245322	41.0	39.0	41.0	35.0	41.0
44-45	38.91547196157038	41.0	38.5	41.0	35.0	41.0
46-47	38.64164555350156	40.0	38.0	41.0	35.0	41.0
48-49	38.52100631858119	40.0	38.0	41.0	35.0	41.0
50-51	38.35791754668681	40.0	37.5	41.0	35.0	41.0
52-53	38.102309202119955	40.0	37.0	41.0	34.5	41.0
54-55	37.9024549817556	40.0	36.0	41.0	34.0	41.0
56-57	37.68110924251672	39.5	35.5	41.0	34.0	41.0
58-59	37.41913182079643	39.0	35.0	41.0	34.0	41.0
60-61	37.16865430467065	39.0	35.0	41.0	33.0	41.0
62-63	36.956935729622685	38.5	35.0	41.0	33.0	41.0
64-65	36.693221686038086	37.5	35.0	41.0	33.0	41.0
66-67	36.41372216684743	37.0	35.0	40.0	33.0	41.0
68-69	36.15724660908664	36.5	35.0	40.0	33.0	41.0
70-71	35.84846162965825	36.0	35.0	39.0	32.0	41.0
72-73	35.56163580558617	35.5	35.0	39.0	32.0	41.0
74-75	35.26116509543736	35.0	35.0	39.0	32.0	40.5
76-77	33.43728402000518	34.5	32.0	36.5	28.5	39.0
78-79	34.73453835716889	35.0	34.5	37.0	31.5	39.0
80-81	34.65176011346222	35.0	35.0	37.0	32.0	39.0
82-83	34.450961838228515	35.0	35.0	36.0	32.0	38.0
84-85	34.234597635032785	35.0	35.0	36.0	32.0	37.0
86-87	34.030172432719624	35.0	35.0	36.0	31.5	37.0
88-89	33.87858356641975	35.0	34.5	35.0	31.0	36.5
90-91	33.734793032435974	35.0	34.0	35.0	31.0	36.0
92-93	33.634284120997094	35.0	34.0	35.0	31.0	36.0
94-95	33.524174391084614	35.0	34.0	35.0	31.0	36.0
96-97	33.46661558524815	35.0	34.0	35.0	31.0	36.0
98-99	33.421707114660954	35.0	34.0	35.0	31.0	35.0
100-101	32.58314708374864	34.5	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	5.0
15	11.0
16	30.0
17	78.0
18	160.0
19	253.0
20	343.0
21	613.0
22	909.0
23	827.0
24	651.0
25	697.0
26	841.0
27	1091.0
28	1334.0
29	1525.0
30	1811.0
31	2009.0
32	2467.0
33	3296.0
34	5527.0
35	11320.0
36	30635.0
37	61258.0
38	72674.0
39	27367.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.669296062964335	19.146063846683255	13.052129105122912	23.132510985229498
2	15.929498860547728	19.456312214313627	39.78247219429088	24.83171673084776
3	20.285763213474954	26.011126674599655	28.275101979880652	25.42800813204473
4	12.943650901682174	16.059031970527922	38.763332030683976	32.23398509710592
5	13.37615976042961	38.205681014837026	34.860214015043404	13.557945209689956
6	30.597476958474758	36.69036317571276	18.664184314638117	14.04797555117436
7	27.48209588962901	31.109901159650654	22.314383444351257	19.093619506369077
8	26.181934741658285	35.05605051352194	19.879600072011627	18.882414672808146
9	26.468224869478924	15.746396125423178	20.239658208227766	37.54572079687013
10-11	26.443855080991124	25.030626896342778	27.434234503229543	21.09128351943655
12-13	26.554946188872446	23.046135741917354	27.772996517974363	22.625921551235834
14-15	23.53572698811369	25.14523076652864	24.97069038952143	26.34835185583623
16-17	23.02879147803865	28.052480668829944	26.209817292450634	22.708910560680774
18-19	22.933288252883756	26.857043747063553	27.673980530514925	22.535687469537766
20-21	23.893360381840616	25.61221398179495	27.587501481959613	22.906924154404823
22-23	24.092703425442505	26.198641415316793	26.801968937853754	22.906686221386952
24-25	23.510479009049753	25.960411168827747	26.993602381652842	23.535507440469654
26-27	23.439705801635654	26.849991766836816	27.05658927493276	22.653713156594762
28-29	23.24022051994757	26.824218552744533	27.40537775348048	22.530183173827417
30-31	23.22221295243281	25.83395172586403	27.600651617407497	23.34318370429567
32-33	23.11243913041569	26.30927237519814	27.86981702899346	22.708471465392705
34-35	23.846712876460888	25.949646400663944	27.071591515372408	23.13204920750276
36-37	23.087681242150932	25.789956703873816	27.60896484495069	23.513397209024564
38-39	23.311349295910706	26.18303247987846	26.978014498926413	23.527603725284425
40-41	23.268757052968063	26.21464734061939	27.46606891161451	23.050526694798037
42-43	23.331986774449923	26.195546695588412	27.582429162952653	22.890037367009015
44-45	23.61326105142511	25.86559341646192	27.207375740768796	23.313769791344175
46-47	23.541484015706335	25.68121941626401	26.915006053574686	23.86229051445497
48-49	23.203265134067653	25.989343087167576	27.112995330190852	23.69439644857392
50-51	23.616300973474253	26.132975617038657	26.82103793344194	23.429685476045155
52-53	23.681287076108386	26.395554599303594	26.329690306093323	23.593468018494693
54-55	23.83562906986445	25.80453234156344	26.618175910354307	23.7416626782178
56-57	23.906341943246588	26.08707107789322	26.230228085121333	23.77635889373886
58-59	24.194908739484724	26.073711260954557	26.51387028048057	23.217509719080144
60-61	23.72214681969719	26.539199796193564	26.448497147149126	23.290156236960122
62-63	23.564926824770243	26.197303076740685	26.819501099933696	23.418268998555376
64-65	23.874269455214474	25.76984381380603	26.329690306093323	24.026196424886166
66-67	23.485670125273884	26.15097852384946	26.04515655942496	24.318194791451692
68-69	23.814552496037162	25.985878695535717	26.72531516064301	23.474253647784103
70-71	24.108087696110932	25.84953960859046	26.409166553233714	23.63320614206489
72-73	23.50520986559293	26.244066724919975	26.595342955374747	23.655380454112347
74-75	23.705656864596186	25.671486469278697	26.63178786428443	23.991068801840687
76-77	23.92240291032996	25.481414153389494	26.493750562592073	24.102432373688472
78-79	23.75763271203076	25.238175746144947	26.481366411895284	24.522825129929014
80-81	23.25968439935097	26.1375038698076	26.49670984017969	24.106101890661744
82-83	23.193267717140493	26.134579792173916	26.7750073000393	23.89714519064629
84-85	23.584880805490542	25.741974294910356	26.976073277508704	23.697071622090395
86-87	23.062382267575888	26.642984794130175	26.594245217154576	23.700387721139364
88-89	23.67931114731208	25.82055931957794	25.957117954167234	24.543011578942746
90-91	23.44812747814403	25.877861254670876	26.242529891411735	24.431481375773355
92-93	23.6191550928467	26.056353489270705	26.695895776342425	23.62859564154017
94-95	23.613885949389875	25.536464668197645	26.40565379092917	24.443995591483308
96-97	23.845289166201958	26.074795491369585	26.499839730219854	23.580075612208606
98-99	22.32689766006121	26.66713503497394	26.8291611962712	24.176806108693647
100-101	23.259017430696833	26.057507678382336	26.331688541085384	24.351786349835447
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.5
2	2.0
3	3.0
4	3.5
5	2.5
6	2.5
7	7.5
8	16.5
9	19.5
10	17.5
11	16.5
12	17.0
13	28.0
14	35.5
15	31.0
16	34.0
17	39.5
18	46.0
19	46.0
20	54.5
21	65.5
22	72.5
23	102.5
24	130.0
25	170.0
26	235.5
27	304.5
28	389.5
29	508.0
30	706.5
31	1156.5
32	1953.5
33	3066.5
34	4325.0
35	5777.0
36	7534.0
37	8656.5
38	8862.5
39	8763.5
40	7977.0
41	7111.0
42	6718.0
43	6317.0
44	6030.0
45	6154.5
46	6548.0
47	6862.0
48	7171.5
49	8061.0
50	9121.5
51	9012.5
52	8507.5
53	9116.5
54	10016.0
55	9843.5
56	8391.0
57	7321.5
58	7022.5
59	6622.5
60	5520.5
61	4103.5
62	3357.0
63	2458.5
64	1483.5
65	915.0
66	703.5
67	632.5
68	478.5
69	299.0
70	185.0
71	133.5
72	91.5
73	66.0
74	56.0
75	43.5
76	36.5
77	21.5
78	10.0
79	5.5
80	3.0
81	2.0
82	2.5
83	2.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47114924409746156
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.781905761369275E-4
22-23	0.001756381152273855
24-25	0.0
26-27	0.001536833508239623
28-29	0.0028541193724450145
30-31	0.0
32-33	0.0
34-35	0.005049595812787333
36-37	0.003073667016479246
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.00944054869347197
46-47	0.08320855708897387
48-49	6.586429321026956E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.00834281047330081
58-59	0.042153147654572516
60-61	0.03249305131706631
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.1954764403423187E-4
78-79	0.009001453405403507
80-81	0.007245072253129651
82-83	0.0010977382201711593
84-85	0.001756381152273855
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06762067436254342
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	227741.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.969675886147485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.48508123969879	38.18995854494363
2	15.763626594557104	16.38461129818775
3	4.665028775036543	7.273201003146022
4	1.9262553313526363	4.004274609774406
5	1.04299313086291	2.7102007481211814
6	0.6294554628925771	1.9627557834775304
7	0.4627425218215182	1.6834005214461982
8	0.3051662093549504	1.26875111932648
9	0.1830912719253583	0.8563674653589028
>10	1.2983486545033114	12.794620239463656
>50	0.1663538287232981	5.944320710105888
>100	0.07087263708921576	6.659992368749446
>500	9.843421817946633E-4	0.2675455878989139
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	523	0.2296468356598065	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	385	0.16905168590635855	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	363	0.15939158956885233	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	359	0.1576352084165785	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	352	0.15456154140009923	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	326	0.14314506391031917	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	317	0.13919320631770302	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	308	0.13524134872508684	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	291	0.12777672882792296	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	286	0.12558125238758064	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	269	0.11811663249041673	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	250	0.10977382201711593	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	250	0.10977382201711593	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	249	0.10933472672904747	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	248	0.10889563144097902	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	241	0.10582196442449976	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	240	0.1053828691364313	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	229	0.10055282096767819	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.3909528806846375E-4	0.0
3	0.0	0.0	0.0	4.3909528806846375E-4	0.0
4	0.0	0.0	0.0	4.3909528806846375E-4	0.0
5	0.0	0.0	0.0	4.3909528806846375E-4	0.0
6	0.0	0.0	0.0	4.3909528806846375E-4	0.0
7	0.0	0.0	0.0	4.3909528806846375E-4	0.0
8	0.0	0.0	0.0	4.3909528806846375E-4	0.0
9	0.0	0.0	0.0	4.3909528806846375E-4	0.0
10-11	0.0	0.0	0.0	8.781905761369275E-4	0.0
12-13	0.0	0.0	0.0	8.781905761369275E-4	0.0
14-15	0.0	0.0	0.0	8.781905761369275E-4	0.0
16-17	0.0	0.0	0.0	8.781905761369275E-4	0.0
18-19	0.0	0.0	0.0	8.781905761369275E-4	0.0
20-21	0.0	0.0	0.0	0.0010977382201711593	0.0
22-23	0.0	0.0	0.0	0.0013172858642053911	0.0
24-25	0.0	0.0	0.0	0.001756381152273855	0.0
26-27	0.0	0.0	0.0	0.0021954764403423186	0.0
28-29	0.0	0.0	0.0	0.0030736670164792463	0.0
30-31	0.0	0.0	0.0	0.005269143456821565	0.0
32-33	0.0	0.0	0.0	0.013392406286088144	0.0
34-35	0.0	0.0	0.0	0.023930693199731276	0.0
36-37	0.0	0.0	0.0	0.039738123570195974	0.0
38-39	0.0	0.0	0.0	0.06344926912589302	0.0
40-41	0.0	0.0	0.0	0.0957227727989251	0.0
42-43	0.0	0.0	0.0	0.12909401469212833	0.0
44-45	0.0	0.0	0.0	0.1686125906182901	0.0
46-47	0.0	0.0	0.0	0.21098528591689683	0.0
48-49	0.0	0.0	0.0	0.2638962681291467	0.0
50-51	0.0	0.0	0.0	0.3172463456294651	0.0
52-53	0.0	0.0	0.0	0.3747678283664338	0.0
54-55	0.0	0.0	0.0	0.4309720252391972	0.0
56-57	0.0	0.0	0.0	0.5091309865153837	0.0
58-59	0.0	0.0	0.0	0.5894854242319125	0.0
60-61	0.0	0.0	0.0	0.6762067436254342	0.0
62-63	0.0	0.0	0.0	0.7607325865786134	0.0
64-65	0.0	0.0	0.0	0.8549185258692988	0.0
66-67	0.0	0.0	0.0	0.9684246578349968	0.0
68-69	0.0	0.0	0.0	1.0981773154592278	0.0
70-71	0.0	0.0	0.0	1.2279299730834587	0.0
72-73	0.0	0.0	0.0	1.3603172024361005	0.0
74-75	0.0	0.0	0.0	1.5194892443609187	0.0
76-77	0.0	0.0	0.0	1.6810763103701134	0.0
78-79	0.0	0.0	0.0	1.8841578811017778	0.0
80-81	0.0	0.0	0.0	2.088556737697648	0.0
82-83	0.0	0.0	0.0	2.3002006665466475	0.0
84-85	0.0	0.0	0.0	2.5201874058689477	0.0
86-87	0.0	0.0	0.0	2.753566551477336	0.0
88-89	0.0	0.0	0.0	3.0218537724871672	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATTG	25	0.0050199763	56.988934	9
TGTACGC	25	0.0050199763	56.988934	2
GTACGCT	25	0.0050199763	56.988934	3
GGTATCA	280	0.0	54.514584	1
GTACTAG	45	9.411797E-4	42.400234	1
CTACACT	80	2.255365E-7	41.554432	4
GTATCAA	460	0.0	38.367603	1
AAACCGT	75	6.9070775E-6	37.992622	6
ACCGTGC	90	5.6798854E-7	36.93727	8
GTCTAAT	130	1.9463187E-10	36.692513	1
CCGTGCG	80	1.0752503E-5	35.618084	9
AACCGTG	95	8.672414E-7	34.993206	7
TATACCG	55	0.0025777551	34.538746	5
ATAATAC	85	1.628647E-5	33.522903	3
GTACATG	970	0.0	32.455853	1
TCGACGC	30	0.003935256	31.688353	58-59
TTCGGCG	30	0.003952224	31.660519	32-33
CCGCATT	30	0.003952224	31.660519	66-67
GTTATTA	170	1.07320375E-10	30.729328	8
GTATAGT	95	3.380142E-5	30.126482	1
>>END_MODULE