FastQCFastQC Report
Fri 10 Feb 2017
SRR3224541_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224541_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038597
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC26130.2515894037822178No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC17340.16695599929520305No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA13950.13431581258178102No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT13400.12902020706780395No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA11970.11525163273146369No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG10940.105334407859834No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG504.2336433E-757.0154349
ACCGTGC851.2914825E-1050.278678
GGTATCA7650.038.5331461
CTCGTCC3650.035.1465039
ATCAACG12650.034.9068643
GTATCAA13650.033.7865071
TCAACGC13350.033.076544
CCTCGTC3800.032.490028
AACCGTG1201.5109254E-731.6538937
CGCAGAG15100.029.2459818
CAACGCA15350.028.7668935
TTAGCGC504.9564376E-528.49879356-57
CATGGGC5950.027.9299054
ACGCAGA16050.027.512267
AACGCAG16200.027.2575196
ACATGGG29450.026.7633933
AAACCGT1551.4153811E-624.5062396
GTACATG34000.024.3318161
GCGCTTA601.7083246E-423.75643258-59
GCAGAGT19300.023.1409829
Produced by FastQC (version 0.11.5)