FastQCFastQC Report
Fri 10 Feb 2017
SRR3224541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038597
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC38230.3680927250897124No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT17820.17157761865285573No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC16820.1619492449910793No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA13870.1335455426888389No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA10640.10244589576130106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA601.494247E-647.49037
CCGTGCG857.234121E-944.696759
ACCGTAT554.968966E-543.1729978
ATGCGGG802.2659515E-741.5540128
CGCACTG608.300184E-539.575255
AATGCGG853.6460915E-739.1096577
AAATGCG853.6460915E-739.1096576
GGTATCA6750.033.2160381
AACCGTG1201.5082878E-731.6601987
TCGCACT600.003954730531.6601984
GTATCAA12300.031.4147571
ACCGTGC1252.1574306E-730.393798
TCAACGC12050.030.34654
ATCAACG12150.030.0967313
GGACGAC650.005849652529.22489
CGGATCG458.960669E-426.383536-37
TTCGCAC908.9576404E-426.38353
TGCGCGG751.0626918E-625.3281612-13
CAACGCA14600.025.0462535
CGCAGAG15200.024.6824578
Produced by FastQC (version 0.11.5)