##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224541_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1038597
Sequences flagged as poor quality	0
Sequence length	101
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.19090369026677	34.0	33.0	34.0	31.0	34.0
2	33.32133156556393	34.0	33.0	34.0	31.0	34.0
3	33.45203673802254	34.0	34.0	34.0	31.0	34.0
4	36.65624010082833	37.0	37.0	37.0	35.0	37.0
5	36.69847496189571	37.0	37.0	37.0	35.0	37.0
6	36.73908647916372	37.0	37.0	37.0	37.0	37.0
7	36.71741686140052	37.0	37.0	37.0	36.0	37.0
8	36.708297828705454	37.0	37.0	37.0	36.0	37.0
9	38.575361762069406	39.0	39.0	39.0	38.0	39.0
10-11	38.603213277142146	39.0	39.0	39.0	37.5	39.0
12-13	38.6140235336709	39.0	39.0	39.0	38.0	39.0
14-15	40.25990494869521	41.0	40.0	41.0	39.0	41.0
16-17	40.27560304911337	41.0	40.0	41.0	39.0	41.0
18-19	40.28977408946877	41.0	40.0	41.0	39.0	41.0
20-21	40.306266530714026	41.0	40.0	41.0	39.0	41.0
22-23	40.241053074484135	41.0	40.0	41.0	39.0	41.0
24-25	40.23028181286871	41.0	40.0	41.0	39.0	41.0
26-27	40.206323530686106	41.0	40.0	41.0	39.0	41.0
28-29	40.18627051686073	41.0	40.0	41.0	39.0	41.0
30-31	40.13555450285337	41.0	40.0	41.0	38.5	41.0
32-33	40.10971002227043	41.0	40.0	41.0	38.0	41.0
34-35	40.06434305125087	41.0	40.0	41.0	38.0	41.0
36-37	40.010911354452205	41.0	40.0	41.0	38.0	41.0
38-39	39.958586439205966	41.0	40.0	41.0	38.0	41.0
40-41	39.886314422244624	41.0	40.0	41.0	38.0	41.0
42-43	39.86146744117305	41.0	40.0	41.0	38.0	41.0
44-45	39.728983908099096	41.0	40.0	41.0	37.5	41.0
46-47	39.603734172157246	41.0	40.0	41.0	37.0	41.0
48-49	39.59775495211328	41.0	40.0	41.0	37.0	41.0
50-51	39.56335951288132	41.0	40.0	41.0	37.0	41.0
52-53	39.45532338337199	41.0	39.5	41.0	36.0	41.0
54-55	39.37048633878203	41.0	39.0	41.0	36.0	41.0
56-57	39.26832351720638	41.0	39.0	41.0	35.5	41.0
58-59	39.12867936264018	41.0	39.0	41.0	35.0	41.0
60-61	39.00179376601319	41.0	39.0	41.0	35.0	41.0
62-63	38.8753948836748	40.0	38.0	41.0	35.0	41.0
64-65	38.71389191380295	40.0	38.0	41.0	35.0	41.0
66-67	38.51340221471851	40.0	37.0	41.0	35.0	41.0
68-69	38.332754186657574	40.0	37.0	41.0	35.0	41.0
70-71	38.078387478492616	39.5	36.5	41.0	35.0	41.0
72-73	37.810652736335655	39.0	36.0	41.0	34.5	41.0
74-75	37.48724577482893	39.0	35.5	40.5	34.0	41.0
76-77	35.430886089599724	36.0	34.0	38.5	31.5	39.5
78-79	36.653983691460695	37.0	35.0	39.0	34.0	40.5
80-81	36.43080665551701	37.0	35.0	39.0	34.0	39.5
82-83	36.05093024532134	36.5	35.0	38.5	34.0	39.0
84-85	35.664849792556694	36.0	35.0	37.0	34.0	39.0
86-87	35.29610041238324	36.0	35.0	37.0	34.0	38.0
88-89	34.998603885819044	35.0	35.0	36.5	34.0	37.0
90-91	34.76132031962349	35.0	35.0	36.0	33.5	37.0
92-93	34.57365802134996	35.0	35.0	36.0	33.0	36.5
94-95	34.39262533976124	35.0	35.0	36.0	33.0	36.0
96-97	34.28781230833519	35.0	35.0	35.0	33.0	36.0
98-99	34.20302484986958	35.0	35.0	35.0	33.0	36.0
100-101	33.49951087861798	35.0	34.0	35.0	31.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	2.0
13	4.0
14	12.0
15	33.0
16	81.0
17	149.0
18	271.0
19	426.0
20	652.0
21	826.0
22	1139.0
23	1236.0
24	1216.0
25	1522.0
26	1963.0
27	2624.0
28	3481.0
29	3982.0
30	4386.0
31	5281.0
32	6772.0
33	9311.0
34	14352.0
35	26003.0
36	61529.0
37	148072.0
38	434217.0
39	308995.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.65213331591654	24.50108129290701	15.223926810742295	21.622858580434162
2	12.370438196913721	23.226044365620158	44.61027713347911	19.793240303987012
3	15.548090356509793	31.686881437169568	32.401691897819845	20.363336308500795
4	10.136848074854827	19.83348690589324	45.25200823803651	24.77765678121543
5	10.168332856728837	41.532278641282424	38.273651859190814	10.025736642797927
6	23.669334689008345	44.3928684561962	21.095670409215508	10.842126445579952
7	21.601448877668624	34.65270937620655	28.317913492914	15.42792825321082
8	21.076991364311663	40.62952232675426	23.777942743913183	14.515543565020888
9	23.974168999140186	17.717940644927722	23.94730583662383	34.360584519308254
10-11	21.20591528764285	29.16058875579267	32.61471966508665	17.01877629147783
12-13	21.615650728819745	26.865280758561788	32.674223014316425	18.84484549830204
14-15	19.47343387281111	29.277525353914946	28.792110895756483	22.45692987751746
16-17	18.94931335253231	32.45840301868771	29.632716058297877	18.959567570482104
18-19	19.0988419954997	31.2274635878979	30.94212673443116	18.73156768217124
20-21	19.735948938317154	30.626562820447393	30.78889876341606	18.848589477819385
22-23	19.76437137697643	30.44179885391979	30.46505167856027	19.328778090543512
24-25	19.796840435493227	30.73500915903013	29.73904587217797	19.72910453329867
26-27	19.268207786930823	31.117056231455937	30.104994976282374	19.50974100533087
28-29	19.51704236350559	30.68612375416322	30.47371405957678	19.32311982275441
30-31	19.98153268152108	30.40849072999346	30.104473221666073	19.50550336681939
32-33	19.599401306861044	30.42989306231348	30.62977838821755	19.340927242607926
34-35	19.575236542713938	30.402833938902578	30.366533484761753	19.65539603362173
36-37	19.81429469632123	30.03424865272906	30.011862339095362	20.139594311854346
38-39	19.6181001870793	30.529358355550805	29.993635645009565	19.858905812360327
40-41	19.446811419636298	30.49363708926561	30.75259219889909	19.306959292198993
42-43	19.60736455044642	30.24455106263546	30.301358467239943	19.846725919678182
44-45	19.639271996156037	30.44546470860684	29.893279897853752	20.02198339738337
46-47	20.232437578532718	29.80600972842133	29.808611425884386	20.152941267161566
48-49	19.691755019668502	29.880669889306606	30.098224563217713	20.329350527807186
50-51	19.487539440225614	30.27983905210587	29.9190157491308	20.31360575853772
52-53	19.63567196901204	29.844636562593575	30.397931055067556	20.121760413326825
54-55	19.985278216671144	30.11139065489309	29.769775957373263	20.1335551710625
56-57	20.3994588504906	30.02782779505647	29.41908274195257	20.15363061250036
58-59	19.773133247253742	29.57093932544875	30.138660057706396	20.517267369591107
60-61	20.048271840190544	29.940579984426265	30.00660222395264	20.004545951430554
62-63	19.728008072428477	30.0924709006477	30.21913215616837	19.96038887075545
64-65	20.151175094863554	29.985981087948453	29.698188999197956	20.164654817990037
66-67	20.18357457223543	30.0930004611991	29.537106307836435	20.186318658729036
68-69	20.23123502186122	29.597861345642247	30.122896561418916	20.048007071077617
70-71	20.31793852668552	29.354648626945774	30.013566378489443	20.313846467879266
72-73	19.744857726336587	30.13676141949187	29.53739515904629	20.58098569512525
74-75	19.97122079112495	29.544375729951078	29.75774049029604	20.72666298862793
76-77	19.68656664683216	29.455590923883634	30.15673111918613	20.701111310098074
78-79	19.944748641464614	29.135655180681475	29.89028204865259	21.029314129201325
80-81	19.590387490160488	29.944465440749894	29.523683431347298	20.941463637742316
82-83	19.84133288464788	29.64674686197001	29.51762943877249	20.994290814609624
84-85	20.054709367318228	29.146218053706075	29.74304313875016	21.056029440225537
86-87	19.38042378323835	30.11967105624222	30.039899980454404	20.46000518006503
88-89	19.766569708943894	29.11586496013372	30.096514817585646	21.021050513336743
90-91	19.884421002564036	29.3379915405109	29.640852034042076	21.136735422882985
92-93	20.43107191721139	29.398554010843476	29.330770260264565	20.839603811680565
94-95	19.97945305060577	28.93672906815637	29.550874882172778	21.532942999065085
96-97	20.077469894482654	28.966577026507878	29.91940088407727	21.036552194932202
98-99	19.588155944991176	29.04615553482246	30.0539574060006	21.311731114185772
100-101	19.808545272621377	29.111890358904148	29.691476646733705	21.388087721740774
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.5
3	2.5
4	2.5
5	1.0
6	3.5
7	7.5
8	11.5
9	16.0
10	16.5
11	13.5
12	18.5
13	27.0
14	29.5
15	27.5
16	31.5
17	41.0
18	51.5
19	59.0
20	71.5
21	84.0
22	87.0
23	116.5
24	165.0
25	261.5
26	475.0
27	991.5
28	2263.5
29	5254.0
30	10476.0
31	19094.0
32	31350.5
33	44612.0
34	56276.0
35	69584.0
36	86964.0
37	97872.5
38	93479.5
39	81321.5
40	67595.0
41	53417.0
42	42268.0
43	33999.5
44	27303.0
45	21618.5
46	16918.0
47	13828.5
48	12399.0
49	12623.5
50	13493.5
51	12905.0
52	11898.5
53	12229.0
54	12786.0
55	12497.5
56	10604.0
57	8797.0
58	8256.0
59	8041.0
60	6859.5
61	4989.5
62	3985.5
63	2779.0
64	1551.0
65	996.0
66	815.0
67	701.5
68	508.0
69	289.0
70	135.5
71	82.5
72	60.0
73	58.0
74	52.5
75	39.0
76	21.5
77	14.0
78	9.5
79	3.5
80	4.0
81	2.5
82	1.0
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4922024615900104
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	9.628373661776415E-4
22-23	0.0012998304443398161
24-25	4.3327681477993874E-4
26-27	0.0022626678105174577
28-29	0.0036587819914750378
30-31	5.295605513977029E-4
32-33	1.4442560492664624E-4
34-35	0.004092058806254977
36-37	0.0015405397858842265
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.008280401349127718
46-47	0.07827867787024226
48-49	7.221280246332312E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.006451010353390198
58-59	0.039524473881592186
60-61	0.02989610021981577
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.888512098532925E-4
78-79	0.006788003431552374
80-81	0.0052474636456681465
82-83	7.221280246332312E-4
84-85	0.001733107259119755
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06754304123736156
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1038597.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.369988008051166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.53234495050504	15.434972344279323
2	14.007478781937643	7.387540950054537
3	6.803126529800377	5.38195095024272
4	4.248366899111198	4.4811753673345525
5	2.854158176395796	3.763205844231917
6	2.149196396193429	3.40045699167405
7	1.676305793284753	3.0942914566721944
8	1.2845464832400946	2.7098780287060475
9	1.093664870871639	2.5955936576740757
>10	6.748728952335285	32.493216096407785
>50	0.4001691208310741	7.2164675847871536
>100	0.1891105878991422	9.077020535132114
>500	0.010973535081081615	2.024928718151871
>1k	0.0018289225135136025	0.9393014746516894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	3823	0.3680927250897124	No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT	1782	0.17157761865285573	No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC	1682	0.1619492449910793	No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA	1387	0.1335455426888389	No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA	1064	0.10244589576130106	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	9.628373661776415E-5	0.0
4	0.0	0.0	0.0	9.628373661776415E-5	0.0
5	0.0	0.0	0.0	9.628373661776415E-5	0.0
6	0.0	0.0	0.0	9.628373661776415E-5	0.0
7	0.0	0.0	0.0	9.628373661776415E-5	0.0
8	0.0	0.0	0.0	9.628373661776415E-5	0.0
9	0.0	0.0	0.0	2.888512098532925E-4	0.0
10-11	0.0	0.0	0.0	3.851349464710566E-4	0.0
12-13	0.0	0.0	0.0	3.851349464710566E-4	0.0
14-15	0.0	0.0	0.0	3.851349464710566E-4	0.0
16-17	0.0	0.0	0.0	3.851349464710566E-4	0.0
18-19	0.0	0.0	0.0	3.851349464710566E-4	0.0
20-21	0.0	0.0	0.0	5.295605513977029E-4	0.0
22-23	0.0	0.0	0.0	8.665536295598775E-4	0.0
24-25	0.0	0.0	0.0	0.001251688576030934	0.0
26-27	0.0	0.0	0.0	0.0016849653908108727	0.0
28-29	0.0	0.0	0.0	0.003081079571768453	0.0
30-31	0.0	0.0	0.0	0.0067398615632434915	0.0
32-33	0.0	0.0	0.0	0.017764349405977486	0.0
34-35	0.0	0.0	0.0	0.035721266285190505	0.0
36-37	0.0	0.0	0.0	0.06763932497397931	0.0
38-39	0.0	0.0	0.0	0.12579470189110886	0.0
40-41	0.0	0.0	0.0	0.19198977081582175	0.0
42-43	0.0	0.0	0.0	0.2591958189750211	0.0
44-45	0.0	0.0	0.0	0.3419998324662983	0.0
46-47	0.0	0.0	0.0	0.4481045102190744	0.0
48-49	0.0	0.0	0.0	0.5563274301774412	0.0
50-51	0.0	0.0	0.0	0.6684498414688277	0.0
52-53	0.0	0.0	0.0	0.7877453911382375	0.0
54-55	0.0	0.0	0.0	0.9025156051866123	0.0
56-57	0.0	0.0	0.0	1.0346168918261847	0.0
58-59	0.0	0.0	0.0	1.193773908455349	0.0
60-61	0.0	0.0	0.0	1.3726690910911548	0.0
62-63	0.0	0.0	0.0	1.5484350522868833	0.0
64-65	0.0	0.0	0.0	1.7301224632846042	0.0
66-67	0.0	0.0	0.0	1.9410319883458165	0.0
68-69	0.0	0.0	0.0	2.1923806827864896	0.0
70-71	0.0	0.0	0.0	2.4702555466653573	0.0
72-73	0.0	0.0	0.0	2.7573255073912213	0.0
74-75	0.0	0.0	0.0	3.0454064473515716	0.0
76-77	0.0	0.0	0.0	3.365212878527475	0.0
78-79	0.0	0.0	0.0	3.732150198777774	0.0
80-81	0.0	0.0	0.0	4.145399996341219	0.0
82-83	0.0	0.0	0.0	4.595237613819412	0.0
84-85	0.0	0.0	0.0	5.045027089429297	0.0
86-87	0.0	0.0	0.0	5.492216904150503	0.0
88-89	0.0	0.0	0.0	5.990870376093904	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTA	60	1.494247E-6	47.4903	7
CCGTGCG	85	7.234121E-9	44.69675	9
ACCGTAT	55	4.968966E-5	43.172997	8
ATGCGGG	80	2.2659515E-7	41.554012	8
CGCACTG	60	8.300184E-5	39.57525	5
AATGCGG	85	3.6460915E-7	39.109657	7
AAATGCG	85	3.6460915E-7	39.109657	6
GGTATCA	675	0.0	33.216038	1
AACCGTG	120	1.5082878E-7	31.660198	7
TCGCACT	60	0.0039547305	31.660198	4
GTATCAA	1230	0.0	31.414757	1
ACCGTGC	125	2.1574306E-7	30.39379	8
TCAACGC	1205	0.0	30.3465	4
ATCAACG	1215	0.0	30.096731	3
GGACGAC	65	0.0058496525	29.2248	9
CGGATCG	45	8.960669E-4	26.3835	36-37
TTCGCAC	90	8.9576404E-4	26.3835	3
TGCGCGG	75	1.0626918E-6	25.32816	12-13
CAACGCA	1460	0.0	25.046253	5
CGCAGAG	1520	0.0	24.682457	8
>>END_MODULE