FastQCFastQC Report
Fri 10 Feb 2017
SRR3224539_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224539_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587101
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG21930.37353027843590797No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA14710.2505531416229916No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA13140.2238115758617342No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA13030.22193796297400276No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC9740.16589990478639963No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA9740.16589990478639963No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA9120.155339541237368No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT8700.1481857465751208No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG8390.142905564800605No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG8320.14171326569023046No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA8210.13983965280249905No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA7420.12638370569970073No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG7410.12621337725536153No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA7330.12485074970064777No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT7320.12468042125630854No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA7210.12280680836857713No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA7110.12110352392518493No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC7080.12059253859216727No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC6640.11309808704124164No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC6290.10713659148936895No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC6250.10645527771201208No Hit
CGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCA6240.10628494926767285No Hit
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC6220.10594429237899441No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9200.053.2650261
GGTATCA5000.047.576531
ATGCGGG2100.040.710428
ATCAACG12550.038.2202033
TCAACGC12750.036.8757064
AATGCGG2150.035.342477
TATATCG702.0542867E-433.9224555
AAATGCG2400.031.660966
CGCAGAG15350.030.6322678
ACGCAGA16000.029.6821487
GTAAATG2750.029.3583474
TATAGCA650.0058469229.2255044
TACATGG26350.029.1977732
GTACATG26150.029.1099221
CCGTGCG1004.9436247E-528.5045749
AAACCGT856.410167E-427.9361426
TATCAAC17450.027.760042
TATACAG1205.3833355E-627.703345
ACATGGG27900.027.0650143
GCAGAGT17550.026.5284759
Produced by FastQC (version 0.11.5)