##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224539_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	587101
Sequences flagged as poor quality	0
Sequence length	101
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09477926285256	34.0	31.0	34.0	31.0	34.0
2	32.254276521416244	34.0	31.0	34.0	31.0	34.0
3	32.40145903345421	34.0	31.0	34.0	31.0	34.0
4	35.64734687898675	37.0	37.0	37.0	35.0	37.0
5	35.63357241769304	37.0	37.0	37.0	35.0	37.0
6	35.72739613797285	37.0	37.0	37.0	35.0	37.0
7	35.70027984963405	37.0	37.0	37.0	35.0	37.0
8	35.74024401252936	37.0	37.0	37.0	35.0	37.0
9	37.46273980115858	39.0	39.0	39.0	35.0	39.0
10-11	37.541102808545716	39.0	39.0	39.0	35.0	39.0
12-13	37.533153580048406	39.0	39.0	39.0	35.0	39.0
14-15	39.022848709165885	41.0	40.0	41.0	36.0	41.0
16-17	38.85062876745228	41.0	40.0	41.0	35.5	41.0
18-19	38.91089693255505	41.0	40.0	41.0	36.0	41.0
20-21	38.962282469285526	41.0	40.0	41.0	36.0	41.0
22-23	38.91894750647674	41.0	40.0	41.0	36.0	41.0
24-25	38.848810511308955	41.0	39.0	41.0	35.0	41.0
26-27	38.65826919048	41.0	39.0	41.0	35.0	41.0
28-29	38.584723923141	41.0	39.0	41.0	35.0	41.0
30-31	38.51541216928604	41.0	39.0	41.0	35.0	41.0
32-33	38.31987937339572	40.5	39.0	41.0	34.0	41.0
34-35	38.3082008036096	40.0	38.0	41.0	34.0	41.0
36-37	38.2733558621089	40.0	38.0	41.0	34.0	41.0
38-39	38.13021354077067	40.0	38.0	41.0	34.0	41.0
40-41	38.00815447427274	40.0	38.0	41.0	33.5	41.0
42-43	37.81263445301575	40.0	37.5	41.0	33.0	41.0
44-45	37.57935176400653	40.0	37.0	41.0	33.0	41.0
46-47	37.365345996685406	40.0	36.5	41.0	32.0	41.0
48-49	37.08453315528334	39.5	36.0	41.0	32.0	41.0
50-51	35.73179657333236	38.0	34.0	40.0	29.5	40.5
52-53	35.99442940822789	38.5	35.0	39.5	30.5	40.5
54-55	36.34036051718529	38.5	35.0	41.0	31.0	41.0
56-57	36.08792609789457	37.5	35.0	41.0	31.0	41.0
58-59	35.86371765675753	37.0	35.0	40.0	31.0	41.0
60-61	35.54932456255398	36.5	35.0	40.0	30.5	41.0
62-63	35.225218488811976	36.0	35.0	39.5	30.0	41.0
64-65	34.95696737017992	35.0	35.0	39.0	30.0	41.0
66-67	34.69405689992011	35.0	35.0	39.0	29.0	41.0
68-69	34.389172391121804	35.0	34.0	37.5	29.0	40.5
70-71	34.129321019722326	35.0	34.0	37.0	29.0	40.0
72-73	33.83697523935405	35.0	34.0	36.5	29.0	39.0
74-75	33.60038732688243	35.0	34.0	36.0	28.5	39.0
76-77	33.34739423029427	35.0	34.0	36.0	28.0	38.0
78-79	33.175438297669395	35.0	34.0	35.0	28.0	37.0
80-81	32.9447156451786	35.0	34.0	35.0	27.0	37.0
82-83	32.764313124998935	35.0	33.0	35.0	27.0	36.0
84-85	32.62639988690191	35.0	33.0	35.0	27.0	36.0
86-87	32.461463189468255	35.0	33.0	35.0	26.0	36.0
88-89	32.36931975929184	35.0	33.0	35.0	26.0	35.0
90-91	32.224723684681166	35.0	33.0	35.0	25.0	35.0
92-93	32.12802652354535	35.0	33.0	35.0	25.0	35.0
94-95	32.01937741547025	35.0	33.0	35.0	25.0	35.0
96-97	31.88831308412011	35.0	33.0	35.0	24.5	35.0
98-99	31.835242147432893	35.0	33.0	35.0	24.0	35.0
100-101	30.467198148189155	34.0	29.5	35.0	20.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	7.0
7	469.0
8	2166.0
9	1449.0
10	996.0
11	1064.0
12	1227.0
13	1258.0
14	1289.0
15	1275.0
16	1302.0
17	1388.0
18	1507.0
19	1668.0
20	1920.0
21	2322.0
22	2801.0
23	2890.0
24	3012.0
25	3183.0
26	3309.0
27	3662.0
28	4134.0
29	4668.0
30	5706.0
31	7499.0
32	10138.0
33	14700.0
34	25454.0
35	48890.0
36	113230.0
37	172414.0
38	122333.0
39	17769.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73251497741447	15.970222580072873	11.651677879825867	28.645584562686793
2	18.356466774882005	18.21628646519083	33.02378977382086	30.40345698610631
3	21.71517336880707	23.446732333959574	25.394778751867225	29.44331554536613
4	15.229917850591296	15.610261266800771	33.10435512799331	36.05546575461462
5	16.288509132159543	36.233969964282124	31.256121178468444	16.221399725089892
6	33.00931185605202	32.88820833212684	18.21969303407761	15.882786777743524
7	29.24931144726376	28.369394703807348	20.50498977177692	21.87630407715197
8	28.180841115923837	31.06893021813964	19.27555906053643	21.47466960540009
9	28.28116456963964	14.03063527399885	18.2174787643012	39.47072139206031
10-11	28.185439983920997	22.837041667447338	24.74369827338056	24.233820075251106
12-13	28.717460879814542	20.542462029531546	25.024910534984613	25.7151665556693
14-15	25.929847906963666	22.208922825541542	22.406056800823976	29.455172466670813
16-17	25.929644044440543	24.563737341629217	23.754487900033492	25.752130713896747
18-19	25.75551457278299	24.574358886991188	24.048520573967043	25.62160596625878
20-21	26.56241081157526	22.939837908158182	23.862754402660123	26.634996877606433
22-23	26.956382432992083	23.85809165262058	22.94246871373085	26.243057200656487
24-25	26.07613158208548	23.379669852302346	23.94286298359483	26.601335582017345
26-27	25.836099751150144	24.811659322671908	24.066302050243486	25.285938875934466
28-29	25.702477086566027	24.869145172636394	24.068431155797725	25.359946584999854
30-31	25.769501329413508	23.987865801625276	24.159471709297037	26.08316115966418
32-33	25.3718695761036	24.558806747050337	24.333377050967382	25.735946625878682
34-35	26.2910470259802	23.506858274811318	23.965382446972498	26.23671225223599
36-37	25.040162455642424	23.96714770754577	25.070656960668902	25.922032876142904
38-39	25.47410448822606	24.361309905541063	23.891101816531123	26.27348378970175
40-41	25.839120518231972	24.000933444392775	24.600518163839933	25.559427873535316
42-43	25.873024660077586	24.325882648520437	24.58257570273343	25.218516988668544
44-45	25.677610470756473	23.565078967608077	24.535959369349342	26.2213511922861
46-47	25.492717607362277	23.48480074126939	24.517416935075907	26.505064716292427
48-49	24.836271782878924	24.28117138277741	24.833716856213837	26.048839978129827
50-51	25.23671395552043	24.477049093767512	24.282278517665613	26.003958433046442
52-53	25.539813422222068	24.330140810524938	23.730840179117394	26.3992055881356
54-55	25.655040614817555	23.598665306310156	24.08265358090005	26.663640497972242
56-57	25.562978090652205	23.79692761552101	24.25689957945907	26.383194714367715
58-59	25.855848182344726	24.372253074852644	24.42455112261933	25.347347620183296
60-61	25.36553331323177	24.862738684778517	23.91387672289994	25.857851279089772
62-63	24.9127060320792	24.105061133462872	24.784160925865507	26.198071908592418
64-65	26.162699058458074	23.174915843992526	24.005186957970135	26.65719813957927
66-67	25.0733161559592	24.181171610485297	23.62547645918954	27.120035774365963
68-69	24.964018116133342	24.390948065154035	24.92663102260088	25.718402796111743
70-71	25.888049926673606	23.866506785033582	24.412579777585115	25.8328635107077
72-73	25.312254620584874	24.109480310883477	25.094745197163693	25.48351987136796
74-75	25.37536130921255	23.998596493618646	25.088783701611817	25.53725849555698
76-77	25.55548363910128	23.577970400322943	24.086911792008532	26.779634168567245
78-79	25.816700354252003	22.745465804332625	24.839206727636512	26.59862711377886
80-81	25.25453840549764	24.134220067399742	24.16378733719895	26.447454189903674
82-83	24.842290914048125	24.446527162429284	24.806165223911115	25.905016699611476
84-85	25.319300278133905	23.39757814796042	25.24876586066045	26.034355713245223
86-87	25.249385430113712	24.668515701887806	24.307144348300294	25.77495451969819
88-89	25.72750235337715	23.606268289254544	24.077795620375774	26.588433736992535
90-91	25.21431576508982	23.435831313521867	24.46665905866282	26.883193862725495
92-93	25.066643047064897	23.834278969072518	24.842909775960127	26.25616820790246
94-95	25.417432434964343	23.33601884513908	25.029850059870444	26.21669866002613
96-97	25.89631085622406	24.06059604735812	24.649080822550122	25.3940122738677
98-99	24.50446859718241	24.219932992333465	24.898611302731915	26.37698710775221
100-101	25.620466314621794	23.621342166831102	24.093007325464296	26.665184193082816
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.5
3	1.0
4	0.5
5	1.0
6	0.5
7	4.0
8	7.0
9	6.5
10	8.0
11	10.0
12	11.0
13	10.5
14	11.0
15	9.0
16	10.5
17	15.5
18	14.0
19	14.0
20	21.0
21	27.5
22	25.5
23	28.0
24	44.0
25	68.5
26	93.5
27	104.0
28	112.0
29	156.0
30	178.5
31	187.5
32	251.0
33	378.5
34	589.5
35	960.0
36	1733.0
37	2871.0
38	4597.5
39	6778.0
40	8929.0
41	11273.5
42	13527.5
43	15435.5
44	16930.5
45	19013.5
46	22099.5
47	22868.0
48	23042.0
49	25518.5
50	28337.5
51	29306.0
52	29710.5
53	31138.0
54	33447.0
55	34339.0
56	30610.0
57	27376.5
58	26935.5
59	26544.5
60	22781.0
61	17616.0
62	14565.0
63	10757.5
64	6858.0
65	4482.5
66	3483.0
67	3199.0
68	2518.5
69	1592.0
70	997.0
71	680.0
72	506.5
73	382.5
74	280.5
75	201.0
76	141.5
77	92.5
78	62.5
79	41.0
80	24.0
81	19.0
82	14.5
83	9.5
84	10.0
85	7.0
86	4.0
87	4.0
88	8.0
89	7.0
90	4.0
91	5.5
92	4.5
93	3.0
94	2.5
95	2.0
96	4.0
97	4.5
98	2.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1507406732402091
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03244756864662128
16-17	0.06549128684842982
18-19	0.022738847319285778
20-21	0.036024465977744884
22-23	0.05825232796401301
24-25	0.015499888434868957
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.018736128877314125
38-39	0.003491733108953996
40-41	0.004769196441498141
42-43	0.003151076220275558
44-45	0.009623557105165891
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.013796603991476765
60-61	0.013966932435815984
62-63	0.025123445540034848
64-65	0.04164530464093912
66-67	0.015074067324020909
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.015244395768360128
80-81	0.051694682856953056
82-83	0.04445572397253624
84-85	0.026145416206070166
86-87	0.05254632507864916
88-89	0.03040362731455065
90-91	0.0
92-93	0.003065911998105948
94-95	0.0
96-97	0.0
98-99	6.813137773568773E-4
100-101	0.041560140418769514
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	587101.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.563391778883414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.95181107445792	40.42634746789086
2	12.251659733509005	12.389709420939722
3	3.541740928409678	5.3724730242745435
4	1.435969441660868	2.9042994184481175
5	0.7248705685696962	1.8325957273785762
6	0.40764164603776676	1.2367046552397911
7	0.2746560650584053	0.9721279555396247
8	0.19314012736890235	0.7812655942701802
9	0.12853685419887284	0.5849333385194541
>10	0.8100611252049401	8.172173472418045
>50	0.1146958276709871	4.18575873121932
>100	0.1529277692523215	16.339492564807358
>500	0.010923412089451532	3.718008676172177
>1k	0.0013654265111814415	1.0841099528822915
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2193	0.37353027843590797	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1471	0.2505531416229916	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1314	0.2238115758617342	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1303	0.22193796297400276	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	974	0.16589990478639963	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	974	0.16589990478639963	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	912	0.155339541237368	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	870	0.1481857465751208	No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG	839	0.142905564800605	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	832	0.14171326569023046	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	821	0.13983965280249905	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	742	0.12638370569970073	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	741	0.12621337725536153	No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA	733	0.12485074970064777	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	732	0.12468042125630854	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	721	0.12280680836857713	No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA	711	0.12110352392518493	No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC	708	0.12059253859216727	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	664	0.11309808704124164	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	629	0.10713659148936895	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	625	0.10645527771201208	No Hit
CGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCA	624	0.10628494926767285	No Hit
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC	622	0.10594429237899441	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	8.516422216960965E-5	0.0
12-13	0.0	0.0	0.0	1.703284443392193E-4	0.0
14-15	0.0	0.0	0.0	5.10985333017658E-4	0.0
16-17	0.0	0.0	0.0	5.10985333017658E-4	0.0
18-19	0.0	0.0	0.0	5.10985333017658E-4	0.0
20-21	0.0	0.0	0.0	5.961495551872676E-4	0.0
22-23	0.0	0.0	0.0	6.813137773568772E-4	0.0
24-25	0.0	0.0	0.0	0.001277463332544145	0.0
26-27	0.0	0.0	0.0	0.002214269776409851	0.0
28-29	0.0	0.0	0.0	0.004428539552819702	0.0
30-31	0.0	0.0	0.0	0.012263647992423791	0.0
32-33	0.0	0.0	0.0	0.03432118153435269	0.0
34-35	0.0	0.0	0.0	0.06234021062815427	0.0
36-37	0.0	0.0	0.0	0.11088381726483176	0.0
38-39	0.0	0.0	0.0	0.21214407742449765	0.0
40-41	0.0	0.0	0.0	0.34236017312183087	0.0
42-43	0.0	0.0	0.0	0.45903515749419604	0.0
44-45	0.0	0.0	0.0	0.5838007429726741	0.0
46-47	0.0	0.0	0.0	0.7235552315530036	0.0
48-49	0.0	0.0	0.0	0.875999189236605	0.0
50-51	0.0	0.0	0.0	1.0315090589183122	0.0
52-53	0.0	0.0	0.0	1.1865931074891714	8.516422216960965E-5
54-55	0.0	0.0	0.0	1.3424436340595571	1.703284443392193E-4
56-57	0.0	0.0	0.0	1.519840708838854	1.703284443392193E-4
58-59	0.0	0.0	0.0	1.7119711940534934	1.703284443392193E-4
60-61	0.0	0.0	0.0	1.9323762010284433	1.703284443392193E-4
62-63	0.0	0.0	0.0	2.160871809109506	1.703284443392193E-4
64-65	0.0	0.0	0.0	2.380936159195777	1.703284443392193E-4
66-67	0.0	0.0	0.0	2.616755890383426	1.703284443392193E-4
68-69	0.0	0.0	0.0	2.8925176417686225	1.703284443392193E-4
70-71	0.0	0.0	0.0	3.199960483800913	1.703284443392193E-4
72-73	0.0	0.0	0.0	3.5234993638232606	1.703284443392193E-4
74-75	0.0	0.0	0.0	3.8432910180701447	1.703284443392193E-4
76-77	0.0	0.0	0.0	4.156099206099121	1.703284443392193E-4
78-79	0.0	0.0	0.0	4.513192789656294	1.703284443392193E-4
80-81	0.0	0.0	0.0	4.913720126519968	1.703284443392193E-4
82-83	0.0	0.0	0.0	5.344310433809515	1.703284443392193E-4
84-85	0.0	0.0	0.0	5.77081285843492	1.703284443392193E-4
86-87	0.0	0.0	0.0	6.222183235933851	1.703284443392193E-4
88-89	0.0	0.0	0.0	6.6778969887634325	1.703284443392193E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	920	0.0	53.265026	1
GGTATCA	500	0.0	47.57653	1
ATGCGGG	210	0.0	40.71042	8
ATCAACG	1255	0.0	38.220203	3
TCAACGC	1275	0.0	36.875706	4
AATGCGG	215	0.0	35.34247	7
TATATCG	70	2.0542867E-4	33.922455	5
AAATGCG	240	0.0	31.66096	6
CGCAGAG	1535	0.0	30.632267	8
ACGCAGA	1600	0.0	29.682148	7
GTAAATG	275	0.0	29.358347	4
TATAGCA	65	0.00584692	29.225504	4
TACATGG	2635	0.0	29.197773	2
GTACATG	2615	0.0	29.109922	1
CCGTGCG	100	4.9436247E-5	28.504574	9
AAACCGT	85	6.410167E-4	27.936142	6
TATCAAC	1745	0.0	27.76004	2
TATACAG	120	5.3833355E-6	27.70334	5
ACATGGG	2790	0.0	27.065014	3
GCAGAGT	1755	0.0	26.528475	9
>>END_MODULE