SRR3224539_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3224539_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	587101
Sequences flagged as poor quality	0
Sequence length	101
%GC	51

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	2946	0.5017875970233401	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2008	0.34201951623315235	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	1539	0.2621354758380585	TruSeq Adapter, Index 3 (95% over 21bp)
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1454	0.2476575580692249	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1386	0.23607522385415797	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1223	0.2083116874268652	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1204	0.20507544698442007	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1096	0.18667997499578437	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1087	0.18514701899673142	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1057	0.1800371656665548	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	989	0.1684548314514879	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	988	0.16828450300714867	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	945	0.16096037990056225	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	930	0.15840545323547395	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	928	0.15806479634679552	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	879	0.14971870257417377	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG	849	0.1446088492439972	TruSeq Adapter, Index 3 (96% over 28bp)
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC	801	0.13643308391571468	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	799	0.13609242702703625	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	798	0.13592209858269702	No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT	781	0.1330265150289303	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	760	0.12944961769780666	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	744	0.12672436258837919	No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA	744	0.12672436258837919	No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC	737	0.12553206347800464	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	736	0.12536173503366543	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	707	0.12042221014782807	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	701	0.11940023948179275	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	691	0.11769695503840054	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	682	0.11616399903934757	No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG	678	0.11548268526199071	No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT	674	0.11480137148463382	RNA PCR Primer, Index 3 (95% over 23bp)
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA	671	0.11429038615161616	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	611	0.104070679491263	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC	606	0.10321903726956691	No Hit
CGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCA	598	0.10185640971485314	No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA	593	0.10100476749315705	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	805	0.0	49.205498	1
GGTATCA	410	0.0	46.559418	1
TATAGGA	155	1.8189894E-12	36.7678	2
ATGCGGG	185	0.0	35.939697	8
ATAGCGT	80	1.077995E-5	35.6188	5
CCCTATA	55	0.0025808022	34.539448	2
AATGCCG	180	0.0	34.29959	9
GTATAGG	140	4.3291948E-10	34.088146	1
GGGAACG	85	1.6327907E-5	33.523582	7
TATATTG	115	1.0365147E-7	33.03773	5
ATCAACG	1260	0.0	32.03808	3
TTATATT	95	3.492109E-5	29.994783	4
TCAACGC	1395	0.0	28.937616	4
TAGCGTT	100	4.9549097E-5	28.495043	6
TAAGTAA	235	0.0	28.29295	4
AAATGCG	235	0.0	28.29295	6
AAGTAAT	220	1.8189894E-12	28.063301	5
GTACATG	3140	0.0	27.96531	1
AATGCGG	240	0.0	27.703516	7
GTAAGTA	275	0.0	27.63156	3

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content