##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224539_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	587101
Sequences flagged as poor quality	0
Sequence length	101
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.169263891562096	34.0	33.0	34.0	31.0	34.0
2	33.29776137325605	34.0	34.0	34.0	31.0	34.0
3	33.41667787995592	34.0	34.0	34.0	31.0	34.0
4	36.625255279755955	37.0	37.0	37.0	35.0	37.0
5	36.65062399825584	37.0	37.0	37.0	35.0	37.0
6	36.69599779254336	37.0	37.0	37.0	36.0	37.0
7	36.67687331481295	37.0	37.0	37.0	35.0	37.0
8	36.66765513940531	37.0	37.0	37.0	35.0	37.0
9	38.52863987627342	39.0	39.0	39.0	37.0	39.0
10-11	38.546761119466666	39.0	39.0	39.0	37.5	39.0
12-13	38.525108115980046	39.0	39.0	39.0	37.0	39.0
14-15	40.124450477856456	41.0	40.0	41.0	38.0	41.0
16-17	40.12572879283121	41.0	40.0	41.0	38.0	41.0
18-19	40.105397538072665	41.0	40.0	41.0	38.0	41.0
20-21	40.099931698293815	41.0	40.0	41.0	38.0	41.0
22-23	40.02661296778578	41.0	40.0	41.0	38.0	41.0
24-25	39.98217427665768	41.0	40.0	41.0	38.0	41.0
26-27	39.90708668525518	41.0	40.0	41.0	38.0	41.0
28-29	39.88278507445908	41.0	40.0	41.0	38.0	41.0
30-31	39.82145916971696	41.0	40.0	41.0	38.0	41.0
32-33	39.7851587716594	41.0	40.0	41.0	37.0	41.0
34-35	39.7042110301294	41.0	40.0	41.0	37.0	41.0
36-37	39.55588220766103	41.0	40.0	41.0	36.5	41.0
38-39	39.416510106438245	41.0	39.0	41.0	36.0	41.0
40-41	39.271259970601314	41.0	39.0	41.0	35.0	41.0
42-43	39.149997189580674	41.0	39.0	41.0	35.0	41.0
44-45	38.87356434412477	40.5	38.0	41.0	35.0	41.0
46-47	38.62679079068167	40.0	37.5	41.0	35.0	41.0
48-49	38.48435192581856	40.0	37.0	41.0	35.0	41.0
50-51	38.24236289837694	40.0	36.5	41.0	35.0	41.0
52-53	37.921757074166116	39.0	36.0	41.0	34.5	41.0
54-55	37.658204465671155	39.0	35.0	41.0	34.0	41.0
56-57	37.31860872320095	38.5	35.0	41.0	34.0	41.0
58-59	36.95896191626313	37.0	35.0	41.0	34.0	41.0
60-61	36.630056838601874	37.0	35.0	40.0	33.0	41.0
62-63	36.34281920827932	36.0	35.0	40.0	33.0	41.0
64-65	36.026834394763426	35.5	35.0	39.5	33.0	41.0
66-67	35.69533862146377	35.0	35.0	39.0	33.0	41.0
68-69	35.38460503388684	35.0	35.0	39.0	32.5	41.0
70-71	35.05813905954852	35.0	35.0	37.0	32.0	40.0
72-73	34.75671051488585	35.0	35.0	37.0	32.0	39.5
74-75	34.481339667280416	35.0	35.0	36.5	31.0	39.0
76-77	32.76254170917781	34.5	32.0	35.0	28.5	36.5
78-79	34.075856624328694	35.0	34.5	36.0	31.5	37.0
80-81	34.057829913422054	35.0	35.0	35.0	32.0	37.0
82-83	33.9213602088908	35.0	35.0	35.0	32.0	36.5
84-85	33.79947061919499	35.0	35.0	35.0	32.0	36.0
86-87	33.67522538711397	35.0	34.5	35.0	31.0	36.0
88-89	33.591043960068205	35.0	34.0	35.0	31.0	36.0
90-91	33.49209676018266	35.0	34.0	35.0	31.0	35.0
92-93	33.43659097838362	35.0	34.0	35.0	31.0	35.0
94-95	33.366263215358174	35.0	34.0	35.0	31.0	35.0
96-97	33.33397916201812	35.0	34.0	35.0	31.0	35.0
98-99	33.31913929630507	35.0	34.0	35.0	31.0	35.0
100-101	32.41406589326198	34.5	32.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	2.0
14	8.0
15	26.0
16	68.0
17	92.0
18	221.0
19	307.0
20	443.0
21	590.0
22	867.0
23	981.0
24	1233.0
25	2052.0
26	2979.0
27	4292.0
28	5971.0
29	5782.0
30	5006.0
31	5214.0
32	5967.0
33	8231.0
34	14739.0
35	34194.0
36	101508.0
37	201946.0
38	156860.0
39	27507.0
40	14.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.35695852471472	17.29243532963234	12.603790891728902	25.746815253924034
2	17.282034947990212	19.208790310355457	36.18712964208884	27.32204509956549
3	20.857058666226084	25.843934859589744	27.120887206800877	26.178119267383295
4	14.43278073108375	16.58760588041921	35.724006601930505	33.25560678656654
5	15.222593727484709	36.83659200035429	33.061943345352844	14.878870926808164
6	31.14268243453852	34.0137386923204	19.504650818172685	15.338928054968395
7	28.318466498949924	29.372629240965352	21.273511712635475	21.03539254744925
8	27.697448990889136	31.475504214777356	20.338749210101838	20.488297584231674
9	28.12327010173718	14.367544936901828	18.97475902783337	38.53442593352762
10-11	27.99041391515259	23.05080386509306	25.83235252537468	23.12642969437967
12-13	28.32391700916878	21.177701962694666	25.92041233109806	24.5779686970385
14-15	25.602238797072395	22.876813359200547	23.026872718663398	28.494075125063663
16-17	25.424841722293095	25.12208291248014	24.254514981238323	25.198560383988443
18-19	25.333375347682935	24.74369827338056	24.982839409232824	24.940086969703678
20-21	26.217666090668228	23.31533231760026	24.341228401123153	26.12577319060836
22-23	26.784188916775747	24.27658579666476	23.591346878568952	25.34787840799054
24-25	26.460699132514275	23.6069214902427	24.13741123728707	25.794968139955955
26-27	25.744307932868782	25.32077759930777	24.47031029366927	24.464604174154175
28-29	25.719405466357344	24.898991963643923	24.840141581584856	24.541460988413885
30-31	25.519761674411	24.036614114216416	25.174588142314036	25.269036069058554
32-33	25.436552546414582	24.612246636007495	25.216061999659345	24.735138817918585
34-35	26.535548482868986	23.54199651324312	24.46103885596219	25.461416147925704
36-37	24.648481080574694	24.260383753906947	25.41667021521219	25.67446495030617
38-39	25.254257785287372	24.00370634694882	24.472620554214693	26.269415313549118
40-41	25.46878646093262	24.000810763395055	25.140563548690942	25.389839226981387
42-43	25.66508999303357	24.054890044472756	25.53759915244566	24.742420810048017
44-45	25.50566219006153	23.493233989493266	25.207221847468368	25.79388197297684
46-47	25.493202380871217	23.265311688223143	24.880001909060535	26.361484021845104
48-49	24.85766820275713	23.565546720172918	25.454759140958938	26.122025936111015
50-51	25.19770874176675	23.712870528239606	25.153849167349403	25.93557156264425
52-53	25.45260525872039	24.035302273373745	24.669690564315168	25.842401903590694
54-55	25.297350881705192	23.429784653747824	24.76090144625882	26.511963018288164
56-57	25.771191312679655	23.67277760034068	24.4887043543064	26.06732673267327
58-59	25.74794234388352	24.314718087693933	24.859024678242307	25.07831489018025
60-61	25.370096824041937	25.152102170405545	24.05454722024871	25.423253785303807
62-63	24.74599770737914	24.661770291653397	24.83107676532658	25.761155235640885
64-65	25.494420891805667	24.162196964406466	24.215169110595962	26.128213033191905
66-67	24.99731732700166	24.50583460086084	23.940344165654633	26.55650390648287
68-69	25.06255312118358	24.761923416924855	24.871444606634974	25.30407885525659
70-71	25.949453330857892	24.43353017623884	24.396398575372892	25.220617917530376
72-73	25.067492646069418	24.510007647747152	24.859691943975566	25.562807762207868
74-75	25.26320002861518	24.122340108431086	25.07669038206373	25.53776948089
76-77	25.277358816761353	23.423672778340336	24.384751844019892	26.91421656087842
78-79	25.597462636228297	23.30317204539183	24.4813189658403	26.618046352539576
80-81	24.96405892292598	24.410208628583888	24.302300228932737	26.323432219557397
82-83	24.661490336751005	24.447640971528482	24.99056797403489	25.900300717685624
84-85	25.124809443105462	23.533499689147412	25.27972474642094	26.06196612132619
86-87	24.930378248376346	24.708269956958002	24.53640855661973	25.824943238045922
88-89	25.442555880504376	23.751790577771114	23.911899315449983	26.893754226274524
90-91	25.309955186586297	23.56051173477817	24.224622339256786	26.904910739378746
92-93	25.259900136348033	23.861928238862003	24.722513436796596	26.155658187993367
94-95	25.30893321592026	23.43446868596715	24.674885581867514	26.581712516245076
96-97	25.633408902386474	24.090148032450976	24.568515468377672	25.70792759678488
98-99	24.000555270728547	24.358500496507414	25.13000318514191	26.510941047622126
100-101	24.995611231837746	23.765307426818357	24.399164855767182	26.83991648557672
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	1.5
4	1.5
5	2.0
6	2.5
7	3.0
8	6.5
9	7.0
10	9.0
11	13.5
12	13.0
13	12.5
14	12.5
15	10.5
16	14.0
17	16.5
18	16.5
19	20.5
20	24.5
21	29.5
22	37.5
23	39.0
24	43.0
25	70.0
26	94.5
27	100.5
28	108.0
29	124.0
30	156.0
31	202.0
32	248.0
33	375.0
34	681.5
35	1226.0
36	2295.5
37	3996.5
38	6295.0
39	8989.0
40	11351.0
41	13514.0
42	15624.0
43	17531.5
44	18846.0
45	20299.0
46	22050.5
47	23057.0
48	24060.5
49	26798.0
50	30155.5
51	30286.0
52	28894.0
53	29745.5
54	32465.5
55	33062.5
56	28915.5
57	25584.5
58	25185.5
59	24645.0
60	20894.5
61	15923.0
62	12834.5
63	9297.0
64	5862.5
65	3741.0
66	2980.0
67	2641.0
68	1894.0
69	1215.0
70	759.5
71	480.0
72	366.5
73	258.5
74	198.5
75	139.5
76	99.5
77	59.0
78	32.5
79	20.5
80	12.0
81	10.5
82	5.5
83	1.5
84	1.5
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.46891420726587074
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.66477999526487E-4
22-23	0.0011071348882049255
24-25	3.4065688867843867E-4
26-27	0.0020439413320706315
28-29	0.003236240442445167
30-31	8.516422216960966E-4
32-33	1.7032844433921934E-4
34-35	0.0038323899976324344
36-37	0.0010219706660353157
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.008346093772621747
46-47	0.07238958884416821
48-49	7.66477999526487E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.006557645107059943
58-59	0.03534315220038801
60-61	0.02725255109427509
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	8.516422216960967E-5
78-79	0.005961495551872676
80-81	0.00493952488583736
82-83	9.368064438657063E-4
84-85	0.0010219706660353157
86-87	0.0
88-89	0.0
90-91	0.0
92-93	8.516422216960967E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06404349507154647
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	587101.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.51575568893126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.38537280350161	37.24540223716979
2	12.865462248633538	12.226243220624788
3	3.9240967058035854	5.593692611181094
4	1.6659335554288783	3.1663236725500483
5	0.8180066487495665	1.9434102036952905
6	0.4541729071070543	1.294822133677828
7	0.3165791768165669	1.0529749175273275
8	0.2137399581895015	0.812481250743579
9	0.15315561892876847	0.6549574474265777
>10	0.9008677875098449	8.441888596683045
>50	0.12183571014284457	4.1259831166701035
>100	0.16316640785580142	16.24060451184295
>500	0.014016497591137125	4.639660406534679
>1k	0.003593973741317212	2.561555673672943
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	2946	0.5017875970233401	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2008	0.34201951623315235	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	1539	0.2621354758380585	TruSeq Adapter, Index 3 (95% over 21bp)
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1454	0.2476575580692249	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1386	0.23607522385415797	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1223	0.2083116874268652	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1204	0.20507544698442007	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1096	0.18667997499578437	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1087	0.18514701899673142	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1057	0.1800371656665548	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	989	0.1684548314514879	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	988	0.16828450300714867	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	945	0.16096037990056225	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	930	0.15840545323547395	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	928	0.15806479634679552	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	879	0.14971870257417377	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG	849	0.1446088492439972	TruSeq Adapter, Index 3 (96% over 28bp)
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC	801	0.13643308391571468	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	799	0.13609242702703625	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	798	0.13592209858269702	No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT	781	0.1330265150289303	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	760	0.12944961769780666	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	744	0.12672436258837919	No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA	744	0.12672436258837919	No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC	737	0.12553206347800464	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	736	0.12536173503366543	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	707	0.12042221014782807	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	701	0.11940023948179275	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	691	0.11769695503840054	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	682	0.11616399903934757	No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG	678	0.11548268526199071	No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT	674	0.11480137148463382	RNA PCR Primer, Index 3 (95% over 23bp)
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA	671	0.11429038615161616	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	611	0.104070679491263	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC	606	0.10321903726956691	No Hit
CGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCA	598	0.10185640971485314	No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA	593	0.10100476749315705	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	8.516422216960965E-5	0.0
12-13	0.0	0.0	0.0	1.703284443392193E-4	0.0
14-15	0.0	0.0	0.0	5.10985333017658E-4	0.0
16-17	0.0	0.0	0.0	5.10985333017658E-4	0.0
18-19	0.0	0.0	0.0	5.10985333017658E-4	0.0
20-21	0.0	0.0	0.0	5.961495551872676E-4	0.0
22-23	0.0	0.0	0.0	6.813137773568772E-4	0.0
24-25	0.0	0.0	0.0	0.001277463332544145	0.0
26-27	0.0	0.0	0.0	0.002043941332070632	0.0
28-29	0.0	0.0	0.0	0.004343375330650092	0.0
30-31	0.0	0.0	0.0	0.01243397643676301	0.0
32-33	0.0	0.0	0.0	0.03534315220038801	0.0
34-35	0.0	0.0	0.0	0.06353250973852881	0.0
36-37	0.0	0.0	0.0	0.11224644481954554	0.0
38-39	0.0	0.0	0.0	0.2141028545343987	0.0
40-41	0.0	0.0	0.0	0.34534092089776713	0.0
42-43	0.0	0.0	0.0	0.4639746823800334	0.0
44-45	0.0	0.0	0.0	0.590613880746243	0.0
46-47	0.0	0.0	0.0	0.731986489547795	0.0
48-49	0.0	0.0	0.0	0.8851117610087531	0.0
50-51	0.0	0.0	0.0	1.0419842582451742	0.0
52-53	0.0	0.0	0.0	1.1997083977032912	0.0
54-55	0.0	0.0	0.0	1.3571770444948994	0.0
56-57	0.0	0.0	0.0	1.534659283496366	0.0
58-59	0.0	0.0	0.0	1.7280672320435495	0.0
60-61	0.0	0.0	0.0	1.9501755234618916	0.0
62-63	0.0	0.0	0.0	2.1833551637622826	0.0
64-65	0.0	0.0	0.0	2.4067409185131687	0.0
66-67	0.0	0.0	0.0	2.6453710690324153	0.0
68-69	0.0	0.0	0.0	2.9251355388595828	0.0
70-71	0.0	0.0	0.0	3.2382843837772377	0.0
72-73	0.0	0.0	0.0	3.567869923573627	0.0
74-75	0.0	0.0	0.0	3.8954115220379455	0.0
76-77	0.0	0.0	0.0	4.214777355173982	0.0
78-79	0.0	0.0	0.0	4.576384642506145	0.0
80-81	0.0	0.0	0.0	4.981596011589147	0.0
82-83	0.0	0.0	0.0	5.416359365765004	0.0
84-85	0.0	0.0	0.0	5.849163942830961	0.0
86-87	0.0	0.0	0.0	6.308369428769496	0.0
88-89	0.0	0.0	0.0	6.769363363373593	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	805	0.0	49.205498	1
GGTATCA	410	0.0	46.559418	1
TATAGGA	155	1.8189894E-12	36.7678	2
ATGCGGG	185	0.0	35.939697	8
ATAGCGT	80	1.077995E-5	35.6188	5
CCCTATA	55	0.0025808022	34.539448	2
AATGCCG	180	0.0	34.29959	9
GTATAGG	140	4.3291948E-10	34.088146	1
GGGAACG	85	1.6327907E-5	33.523582	7
TATATTG	115	1.0365147E-7	33.03773	5
ATCAACG	1260	0.0	32.03808	3
TTATATT	95	3.492109E-5	29.994783	4
TCAACGC	1395	0.0	28.937616	4
TAGCGTT	100	4.9549097E-5	28.495043	6
TAAGTAA	235	0.0	28.29295	4
AAATGCG	235	0.0	28.29295	6
AAGTAAT	220	1.8189894E-12	28.063301	5
GTACATG	3140	0.0	27.96531	1
AATGCGG	240	0.0	27.703516	7
GTAAGTA	275	0.0	27.63156	3
>>END_MODULE