FastQCFastQC Report
Fri 10 Feb 2017
SRR3224538_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224538_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889795
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG32160.3614315656977169No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA29100.32704162194662817No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA27100.30456453452761595No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT16440.18476165858428065No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA15690.17633275080215105No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA15510.17430981293443995No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA15320.1721744896296338No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA15080.16947723913935234No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC14540.163408425536219No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG12960.14565152647519933No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC12850.14441528666715367No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA12840.14430290123005862No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA11700.13149096140122163No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC11500.1292432526593204No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA11020.12384875167875747No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG10950.12306205361909205No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC10910.12261251187071179No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC10680.1200276468175254No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG10440.11733039632724392No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC10240.11508268758534268No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT9940.11171112447249086No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA9880.11103681184992048No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG9820.11036249922735011No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11600.045.5181241
GGTATCA6650.032.904521
GTACATG42300.032.724421
TACATGG41600.032.6426852
ATCAACG16050.032.245173
TCAACGC16550.031.2709984
ACATGGG43550.030.6359543
ACCGTGC2051.8189894E-1230.1094448
TCGCACC11450.029.4966181
AATGCGG4250.029.0467577
AAATGCG4350.028.3790156
ATTAGAC1205.397289E-627.6968273
ATACGAC700.00840758327.1315883
CGCACCA12550.026.861352
TATACAG2305.456968E-1226.8366785
CATGGGG33100.026.680764
CATAATA13300.026.4175974
CATGGGA11600.026.1960144
AAACCGT1109.501057E-525.8983336
ACCGTAT1658.439565E-825.8983338
Produced by FastQC (version 0.11.5)