##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224538_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	889795
Sequences flagged as poor quality	0
Sequence length	101
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93759910990734	34.0	31.0	34.0	30.0	34.0
2	32.11413752605937	34.0	31.0	34.0	30.0	34.0
3	32.26281446850117	34.0	31.0	34.0	30.0	34.0
4	35.546217949078155	37.0	35.0	37.0	35.0	37.0
5	35.53171460842104	37.0	35.0	37.0	35.0	37.0
6	35.62117004478559	37.0	37.0	37.0	35.0	37.0
7	35.58400755230137	37.0	37.0	37.0	35.0	37.0
8	35.625556448395415	37.0	37.0	37.0	35.0	37.0
9	37.32966919346591	39.0	38.0	39.0	35.0	39.0
10-11	37.39266235481206	39.0	38.0	39.0	35.0	39.0
12-13	37.37845795941762	39.0	38.0	39.0	35.0	39.0
14-15	38.82626054315882	41.0	39.0	41.0	36.0	41.0
16-17	38.64977831972533	41.0	39.0	41.0	35.5	41.0
18-19	38.70049281014166	41.0	39.0	41.0	35.0	41.0
20-21	38.75635118201384	41.0	39.0	41.0	35.0	41.0
22-23	38.707096578425364	41.0	39.0	41.0	35.0	41.0
24-25	38.63391961069684	41.0	39.0	41.0	35.0	41.0
26-27	38.432786203563744	40.5	39.0	41.0	34.0	41.0
28-29	38.343994965132424	40.0	38.5	41.0	34.0	41.0
30-31	38.26985204457206	40.0	38.0	41.0	34.0	41.0
32-33	38.07222506307633	40.0	38.0	41.0	33.0	41.0
34-35	38.05075775880961	40.0	38.0	41.0	33.0	41.0
36-37	38.028726841575875	40.0	38.0	41.0	33.0	41.0
38-39	37.88555847133328	40.0	38.0	41.0	33.0	41.0
40-41	37.76282626897206	40.0	38.0	41.0	33.0	41.0
42-43	37.58450373400615	40.0	37.0	41.0	32.0	41.0
44-45	37.35997055501548	40.0	37.0	41.0	32.0	41.0
46-47	37.135284531830365	40.0	36.0	41.0	31.0	41.0
48-49	36.87226889339679	39.5	35.5	41.0	31.0	41.0
50-51	35.53211357672273	38.0	34.0	40.0	28.5	40.5
52-53	35.843057670587044	38.5	35.0	39.5	29.5	40.5
54-55	36.22234447260324	38.5	35.0	40.5	30.0	41.0
56-57	35.99145196365455	37.5	35.0	40.5	30.0	41.0
58-59	35.78595800156216	37.0	35.0	40.0	30.0	41.0
60-61	35.47652886338988	36.5	35.0	40.0	29.5	41.0
62-63	35.14512387684804	36.0	35.0	40.0	29.0	41.0
64-65	34.87026225141746	35.0	34.5	39.0	29.0	41.0
66-67	34.603279968981624	35.0	34.0	39.0	29.0	41.0
68-69	34.27999539219708	35.0	34.0	38.0	28.0	40.5
70-71	33.99440095752392	35.0	34.0	37.0	28.0	40.0
72-73	33.69054950859468	35.0	34.0	37.0	27.0	39.0
74-75	33.44823976309149	35.0	34.0	36.0	27.0	39.0
76-77	33.18356812524233	35.0	34.0	36.0	27.0	38.5
78-79	33.00556307913621	35.0	33.0	35.0	27.0	37.0
80-81	32.76251215167539	35.0	33.0	35.0	26.0	37.0
82-83	32.572127287746056	35.0	33.0	35.0	26.0	36.0
84-85	32.41479722857512	35.0	33.0	35.0	25.5	36.0
86-87	32.23729173573688	35.0	33.0	35.0	25.0	36.0
88-89	32.1288184357071	35.0	33.0	35.0	25.0	35.5
90-91	31.983287161649596	35.0	33.0	35.0	25.0	35.0
92-93	31.877435251940053	35.0	33.0	35.0	24.0	35.0
94-95	31.747070392618525	35.0	32.0	35.0	24.0	35.0
96-97	31.613448603329978	35.0	32.0	35.0	24.0	35.0
98-99	31.56082637011896	35.0	32.0	35.0	24.0	35.0
100-101	30.18020386718289	34.0	29.5	35.0	19.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	10.0
7	549.0
8	2282.0
9	1598.0
10	1191.0
11	1615.0
12	2224.0
13	2432.0
14	2360.0
15	2360.0
16	2405.0
17	2520.0
18	2792.0
19	2960.0
20	3486.0
21	4232.0
22	4839.0
23	5013.0
24	5054.0
25	5413.0
26	5641.0
27	6178.0
28	7060.0
29	8167.0
30	10134.0
31	12781.0
32	17592.0
33	25374.0
34	42921.0
35	77913.0
36	170663.0
37	245335.0
38	177869.0
39	26830.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87030689831777	16.42280266319978	11.75950157867189	27.947388859810555
2	18.050449822711972	18.480886046786058	33.650784731314516	29.817879399187454
3	21.114526379671723	23.636792744396182	25.857641366831686	29.39103950910041
4	14.518063149377102	15.510876100674874	33.75676419849516	36.21429655145286
5	15.81251861383802	37.15395119100467	31.55007614113363	15.48345405402368
6	33.08953185846178	33.50591990289898	18.14069532869931	15.26385290993993
7	29.551863069583444	28.737181036081346	20.60822998555847	21.10272590877674
8	28.336864109148735	31.47028248079614	19.214088638394237	20.978764771660888
9	28.183008445765594	13.693041655662258	18.144404048123445	39.9795458504487
10-11	27.961384363814133	22.875718564388425	25.19209480835473	23.970802263442703
12-13	28.485662427862597	20.62463825937435	25.52717198905366	25.362527323709394
14-15	25.608161512317256	22.310648349585076	22.619943043005566	29.461247095092098
16-17	25.838806151451315	24.674470892180892	23.977241924617392	25.509481031750408
18-19	25.68577712601729	24.625642103677507	24.124718328740222	25.563862441564982
20-21	26.45296101432793	22.9355425720121	23.92962127417245	26.681875139487516
22-23	26.753862842316405	23.914954205981143	23.10260030849879	26.228582643203662
24-25	25.86406420280154	23.607535140062847	23.949029836946963	26.579370820188654
26-27	25.699571249557486	24.78166319208357	24.314870279109233	25.203895279249718
28-29	25.460190268545002	24.951477587534207	24.24845048578605	25.33988165813474
30-31	25.553133025022618	24.23867295275878	24.266600733876903	25.941593288341696
32-33	25.31549401828511	24.533459954259126	24.308857658224646	25.842188369231117
34-35	26.100618681831207	23.600323670058835	24.084423940345808	26.214633707764147
36-37	24.898351935556757	24.053804622134493	25.17887473878386	25.868968703524885
38-39	25.314357965720706	24.337229558864852	24.086709749929756	26.261702725484685
40-41	25.829466582222853	23.845636410943154	24.67986560171555	25.64503140511844
42-43	25.850702151691742	24.156771731861785	24.70455682118312	25.28796929526336
44-45	25.693024625514365	23.673550360969646	24.461355510421082	26.172069503094907
46-47	25.413718890306193	23.664945296388492	24.45664450800465	26.464691305300658
48-49	24.900960333559976	24.366792351047152	24.755084036210587	25.977163279182285
50-51	25.088700206227276	24.69950943756708	24.2177692614591	25.994021094746543
52-53	25.5285206142988	24.376907040385706	23.59060232975011	26.503970015565383
54-55	25.430351934996263	23.59633398704196	24.24783236588203	26.725481712079745
56-57	25.43271202917526	24.034693384431247	24.17455706089605	26.358037525497448
58-59	25.862815097240926	24.277184408180332	24.45270365965789	25.407296834920857
60-61	25.27828953802194	24.760602991577407	24.095214757579267	25.86589271282138
62-63	24.9543156338618	24.133767646306605	24.765844788384843	26.146071931446752
64-65	26.176047100917604	23.266535443474233	24.042405218792464	26.515012236815693
66-67	25.0793872038506	24.07520230388691	23.714208314271037	27.131202177991447
68-69	25.04610612556825	24.35864440685776	25.09729769216505	25.497951775408943
70-71	25.65866295045488	23.899774667198624	24.59274327232677	25.848819110019722
72-73	25.41978770390933	24.170174028849342	25.007726498800288	25.402311768441045
74-75	25.25385060603847	24.07189296410971	25.080158913008056	25.594097516843767
76-77	25.476542349642333	23.4008395192151	24.041492703375496	27.081125427767073
78-79	25.583545454187796	22.7937791871083	24.867908049052044	26.75476730965186
80-81	25.092930845731143	24.180800045882055	24.206215107004716	26.520054001382082
82-83	24.866907710497777	24.440967406233817	24.65298177890797	26.039143104360434
84-85	25.09321169298741	23.403625039698934	25.310860223213403	26.192303044100246
86-87	25.23941739144125	24.797299272863853	24.25149734268062	25.711785993014274
88-89	25.367637138771215	23.679710477321173	24.075791712761845	26.87686067114577
90-91	24.882248158283648	23.497940536865233	24.598755893211358	27.02105541163976
92-93	24.8068732716654	23.72602668905459	25.047948026755364	26.41915201252465
94-95	25.337690142111384	23.26524648935991	25.133541995628207	26.2635213729005
96-97	25.780488764265925	24.052899825240644	24.717996841969217	25.448614568524206
98-99	24.43132649276796	24.178289259263423	24.91419323660639	26.476191011362232
100-101	25.388482478372037	23.396258874379047	24.369095459871055	26.846163187377865
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	1.5
6	2.5
7	5.0
8	8.0
9	9.5
10	10.0
11	7.5
12	8.5
13	17.0
14	19.0
15	17.5
16	19.5
17	22.0
18	25.0
19	32.5
20	38.0
21	51.0
22	57.5
23	58.5
24	74.5
25	92.5
26	125.5
27	147.5
28	173.0
29	205.0
30	255.5
31	321.0
32	448.0
33	745.5
34	1245.5
35	2139.5
36	3891.5
37	6459.5
38	9491.5
39	12794.5
40	16045.0
41	18850.5
42	20951.5
43	22547.5
44	24038.5
45	26606.5
46	30102.0
47	31055.5
48	32362.5
49	36865.0
50	41814.0
51	44422.0
52	45977.5
53	48385.5
54	52078.5
55	54493.5
56	48457.0
57	41942.0
58	40199.5
59	38517.0
60	33228.0
61	26417.5
62	21705.0
63	15802.0
64	10307.0
65	6911.5
66	5214.0
67	4493.0
68	3395.0
69	2182.0
70	1452.5
71	1029.0
72	762.0
73	585.0
74	464.0
75	348.0
76	220.0
77	139.0
78	89.0
79	63.5
80	50.5
81	39.5
82	30.5
83	21.5
84	15.0
85	15.0
86	12.0
87	9.5
88	8.5
89	7.5
90	6.5
91	5.0
92	5.5
93	5.5
94	3.5
95	3.5
96	2.5
97	4.5
98	5.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1562157575621351
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03955967385746155
16-17	0.0791193477149231
18-19	0.0268039267471721
20-21	0.04253788794048067
22-23	0.07310672683033732
24-25	0.019836029647278305
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02315140004158261
38-39	0.005057344669277756
40-41	0.005787850010395653
42-43	0.003371563112851837
44-45	0.012137627206266613
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.01674543012716412
60-61	0.01843121168359004
62-63	0.03006310442292888
64-65	0.052877348153226304
66-67	0.018880753431970286
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.018936946150517817
80-81	0.0627672666175917
82-83	0.052652577279036175
84-85	0.0323108131648301
86-87	0.06214914671356886
88-89	0.03719957967846527
90-91	0.0
92-93	0.0033153703943043064
94-95	0.0
96-97	0.0
98-99	5.619271854753061E-4
100-101	0.04911243601054175
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	889795.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.3132874290193
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.94572631159387	37.944467180382816
2	13.287381518277819	13.104889279797458
3	4.413991011629814	6.5300522239682595
4	1.9441615206022347	3.834919834955889
5	0.9987269758493766	2.4625255211587764
6	0.5673738974193846	1.6787443249899017
7	0.3459006794590487	1.1940249739639999
8	0.2532937477958036	0.999259791122239
9	0.1723150906577121	0.7647681234565149
>10	0.8486507179307228	7.7056423905241145
>50	0.08853092189558318	3.1143410122610193
>100	0.11573442110136552	12.653825705921044
>500	0.013602252676585606	4.5219114255976285
>1k	0.004610933110706985	3.4906282119003365
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	3216	0.3614315656977169	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2910	0.32704162194662817	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2710	0.30456453452761595	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1644	0.18476165858428065	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1569	0.17633275080215105	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1551	0.17430981293443995	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1532	0.1721744896296338	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1508	0.16947723913935234	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1454	0.163408425536219	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1296	0.14565152647519933	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1285	0.14441528666715367	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1284	0.14430290123005862	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1170	0.13149096140122163	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1150	0.1292432526593204	No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA	1102	0.12384875167875747	No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG	1095	0.12306205361909205	No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC	1091	0.12261251187071179	No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC	1068	0.1200276468175254	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	1044	0.11733039632724392	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1024	0.11508268758534268	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	994	0.11171112447249086	No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA	988	0.11103681184992048	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	982	0.11036249922735011	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	1.1238543709506122E-4
5	0.0	0.0	0.0	0.0	1.1238543709506122E-4
6	0.0	0.0	0.0	0.0	1.1238543709506122E-4
7	0.0	0.0	0.0	0.0	1.1238543709506122E-4
8	0.0	0.0	0.0	0.0	1.1238543709506122E-4
9	0.0	0.0	0.0	0.0	1.1238543709506122E-4
10-11	0.0	0.0	0.0	0.0	1.1238543709506122E-4
12-13	0.0	0.0	0.0	0.0	1.1238543709506122E-4
14-15	0.0	0.0	0.0	0.0	1.1238543709506122E-4
16-17	0.0	0.0	0.0	0.0	2.2477087419012245E-4
18-19	0.0	0.0	0.0	5.619271854753061E-5	3.371563112851837E-4
20-21	0.0	0.0	0.0	2.2477087419012245E-4	3.371563112851837E-4
22-23	0.0	0.0	0.0	6.181199040228367E-4	5.619271854753062E-4
24-25	0.0	0.0	0.0	0.001461010682235796	7.30505341117898E-4
26-27	0.0	0.0	0.0	0.002303901460448755	7.866980596654286E-4
28-29	0.0	0.0	0.0	0.003933490298327143	7.866980596654286E-4
30-31	0.0	0.0	0.0	0.011631892739338836	7.866980596654286E-4
32-33	0.0	0.0	0.0	0.031692693260807266	7.866980596654286E-4
34-35	0.0	0.0	0.0	0.05551840592496024	7.866980596654286E-4
36-37	0.0	0.0	0.0	0.09451615259694648	7.866980596654286E-4
38-39	0.0	0.0	0.0	0.17616417264650847	8.990834967604898E-4
40-41	0.0	0.0	0.0	0.2778168004989914	8.990834967604898E-4
42-43	0.0	0.0	0.0	0.3732320365926983	9.552762153080205E-4
44-45	0.0	0.0	0.0	0.4743789299782534	0.0010676616524030818
46-47	0.0	0.0	0.0	0.5821003714338696	0.0011238543709506123
48-49	0.0	0.0	0.0	0.6964525536780943	0.0013486252451407347
50-51	0.0	0.0	0.0	0.8247967228406543	0.0013486252451407347
52-53	0.0	0.0	0.0	0.9478025837411987	0.0013486252451407347
54-55	0.0	0.0	0.0	1.0682797723071045	0.0013486252451407347
56-57	0.0	0.0	0.0	1.2066262453711247	0.0014048179636882653
58-59	0.0	0.0	0.0	1.3536263970914648	0.0015172034007833266
60-61	0.0	0.0	0.0	1.5238341415719352	0.0015733961193308572
62-63	0.0	0.0	0.0	1.697076292853972	0.0015733961193308572
64-65	0.0	0.0	0.0	1.859417056737788	0.0015733961193308572
66-67	0.0	0.0	0.0	2.0358060002584866	0.0015733961193308572
68-69	0.0	0.0	0.0	2.2388302923707144	0.0015733961193308572
70-71	0.0	0.0	0.0	2.466354609769666	0.0015733961193308572
72-73	0.0	0.0	0.0	2.706297517967622	0.0016857815564259183
74-75	0.0	0.0	0.0	2.9416888159632277	0.0016857815564259183
76-77	0.0	0.0	0.0	3.174720019779837	0.0016857815564259183
78-79	0.0	0.0	0.0	3.4353980411218314	0.0016857815564259183
80-81	0.0	0.0	0.0	3.7368157834107856	0.0017419742749734488
82-83	0.0	0.0	0.0	4.06621749953641	0.0017981669935209796
84-85	0.0	0.0	0.0	4.389100860310521	0.0017981669935209796
86-87	0.0	0.0	0.0	4.727549604122298	0.0017981669935209796
88-89	0.0	0.0	0.0	5.079934142133863	0.0017981669935209796
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1160	0.0	45.518124	1
GGTATCA	665	0.0	32.90452	1
GTACATG	4230	0.0	32.72442	1
TACATGG	4160	0.0	32.642685	2
ATCAACG	1605	0.0	32.24517	3
TCAACGC	1655	0.0	31.270998	4
ACATGGG	4355	0.0	30.635954	3
ACCGTGC	205	1.8189894E-12	30.109444	8
TCGCACC	1145	0.0	29.496618	1
AATGCGG	425	0.0	29.046757	7
AAATGCG	435	0.0	28.379015	6
ATTAGAC	120	5.397289E-6	27.696827	3
ATACGAC	70	0.008407583	27.131588	3
CGCACCA	1255	0.0	26.86135	2
TATACAG	230	5.456968E-12	26.836678	5
CATGGGG	3310	0.0	26.68076	4
CATAATA	1330	0.0	26.417597	4
CATGGGA	1160	0.0	26.196014	4
AAACCGT	110	9.501057E-5	25.898333	6
ACCGTAT	165	8.439565E-8	25.898333	8
>>END_MODULE