##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224538_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	889795
Sequences flagged as poor quality	0
Sequence length	101
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.1770003202985	34.0	33.0	34.0	31.0	34.0
2	33.30381379980782	34.0	34.0	34.0	31.0	34.0
3	33.41606774594148	34.0	34.0	34.0	31.0	34.0
4	36.631167853269574	37.0	37.0	37.0	35.0	37.0
5	36.657914463443824	37.0	37.0	37.0	35.0	37.0
6	36.697023471698536	37.0	37.0	37.0	36.0	37.0
7	36.67586466545665	37.0	37.0	37.0	36.0	37.0
8	36.66419793323181	37.0	37.0	37.0	35.0	37.0
9	38.513464337291175	39.0	39.0	39.0	37.0	39.0
10-11	38.536338145303134	39.0	39.0	39.0	37.5	39.0
12-13	38.515015818250276	39.0	39.0	39.0	37.0	39.0
14-15	40.12794126737057	41.0	40.0	41.0	38.0	41.0
16-17	40.13209334734405	41.0	40.0	41.0	38.0	41.0
18-19	40.10682797723071	41.0	40.0	41.0	38.0	41.0
20-21	40.101095196084486	41.0	40.0	41.0	38.0	41.0
22-23	40.026562298057414	41.0	40.0	41.0	38.0	41.0
24-25	39.985799538095854	41.0	40.0	41.0	38.0	41.0
26-27	39.91955899954483	41.0	40.0	41.0	38.0	41.0
28-29	39.895479857720034	41.0	40.0	41.0	38.0	41.0
30-31	39.83839198916604	41.0	40.0	41.0	38.0	41.0
32-33	39.806457105288295	41.0	40.0	41.0	37.0	41.0
34-35	39.72324130839126	41.0	40.0	41.0	37.0	41.0
36-37	39.58823774015363	41.0	40.0	41.0	37.0	41.0
38-39	39.46212835540771	41.0	39.0	41.0	36.0	41.0
40-41	39.324333132912635	41.0	39.0	41.0	35.5	41.0
42-43	39.21194488618165	41.0	39.0	41.0	35.0	41.0
44-45	38.94036884900454	40.5	38.5	41.0	35.0	41.0
46-47	38.697167886985206	40.0	38.0	41.0	35.0	41.0
48-49	38.568669749773825	40.0	37.0	41.0	35.0	41.0
50-51	38.34399608898679	40.0	37.0	41.0	35.0	41.0
52-53	38.03298344000584	39.5	36.0	41.0	35.0	41.0
54-55	37.77577419517979	39.0	35.5	41.0	34.0	41.0
56-57	37.45457380632618	39.0	35.0	41.0	34.0	41.0
58-59	37.104893823858305	37.5	35.0	41.0	34.0	41.0
60-61	36.77450143010469	37.0	35.0	40.5	33.0	41.0
62-63	36.49239206783585	36.5	35.0	40.0	33.0	41.0
64-65	36.18127658617996	36.0	35.0	39.5	33.0	41.0
66-67	35.84973224169612	35.0	35.0	39.0	33.0	41.0
68-69	35.53370832607511	35.0	35.0	39.0	32.5	41.0
70-71	35.21958260048663	35.0	35.0	37.5	32.0	40.5
72-73	34.91919599458302	35.0	35.0	37.0	32.0	39.5
74-75	34.63926578593946	35.0	35.0	36.5	32.0	39.0
76-77	32.94045931928141	34.5	32.0	35.0	28.5	36.5
78-79	34.223467765047005	35.0	34.5	36.0	31.5	37.0
80-81	34.20381042824471	35.0	35.0	35.0	32.0	37.0
82-83	34.07228743699392	35.0	35.0	35.0	32.0	36.5
84-85	33.95049590074118	35.0	35.0	35.0	32.0	36.0
86-87	33.82303620496856	35.0	35.0	35.0	32.0	36.0
88-89	33.73249737299041	35.0	34.5	35.0	31.5	36.0
90-91	33.629628172781366	35.0	34.0	35.0	31.0	35.5
92-93	33.57636815221484	35.0	34.0	35.0	31.0	35.0
94-95	33.505449007917555	35.0	34.0	35.0	31.0	35.0
96-97	33.48208857096297	35.0	34.0	35.0	31.0	35.0
98-99	33.46271613124371	35.0	34.0	35.0	31.0	35.0
100-101	32.564157474474456	34.5	32.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	10.0
15	50.0
16	86.0
17	149.0
18	278.0
19	419.0
20	555.0
21	847.0
22	1097.0
23	1451.0
24	1831.0
25	2734.0
26	3892.0
27	5719.0
28	7799.0
29	7698.0
30	7131.0
31	7734.0
32	9506.0
33	12544.0
34	22323.0
35	49529.0
36	149240.0
37	305504.0
38	246358.0
39	45301.0
40	6.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.867628193546054	16.842906443103388	11.987316156542478	26.302149206808078
2	17.653841615203504	19.013368247742456	35.491096263746144	27.8416938733079
3	20.47628948240887	25.157367708292362	26.730089515000643	27.636253294298125
4	14.226760096426705	16.268241561258492	35.29037587309436	34.21462246922044
5	15.40062598688462	36.587191431734276	33.051433195286556	14.960749386094548
6	32.3039576531673	33.574699790401155	19.092937137205762	15.028405419225777
7	29.323608246843374	28.894633033451527	21.037542355261603	20.744216364443496
8	28.25190071870487	31.359358054383314	19.606426199293097	20.782315027618722
9	27.97835456481549	14.315656977168898	18.516849386656478	39.189139071359136
10-11	28.009597716327917	22.9081979557089	25.86090054450744	23.221303783455742
12-13	28.54230468815851	21.067998808714368	25.927207952393527	24.462488550733596
14-15	25.601458762973493	22.920728931944996	23.01856045493625	28.459251850145257
16-17	25.61567552076602	25.165852808793037	24.150787540950443	25.0676841294905
18-19	25.43743221753325	24.739012918705996	24.91365988795172	24.909894975809035
20-21	26.354630668679874	23.472547751408896	24.29425803040154	25.878563549509686
22-23	26.7093774549525	24.24388867847554	23.680886238554795	25.365847628017168
24-25	26.393531057890062	23.53538475370593	24.147664055563673	25.923420132840334
26-27	25.80415308874245	25.052218462830062	24.472350485489812	24.671277962937676
28-29	25.69726511134438	24.910313094962472	24.72610653185908	24.66631526183406
30-31	25.501369709059745	24.17627804391498	24.954890914965652	25.36746133205962
32-33	25.611040308206167	24.675711456809104	24.907338102976645	24.805910132008083
34-35	26.385991728106458	23.71566489839957	24.34881316310016	25.54953021039381
36-37	24.744907993800695	24.304629137413084	25.28341370927772	25.667049159508505
38-39	25.405627138835353	24.159104063295477	24.44821560022252	25.98705319764665
40-41	25.505088250664482	24.049134913097962	24.983732207980488	25.46204462825707
42-43	25.702774234514692	24.151012311824633	25.334992891621106	24.811220562039573
44-45	25.660259450662338	23.565344836500227	25.065694382315456	25.708701330521983
46-47	25.40079946192658	23.41901567656209	24.88623301368353	26.293951847827802
48-49	25.03339280446174	23.704086649902365	25.2957026256269	25.96681792000899
50-51	25.27885636579212	24.044077568428683	24.896240145201983	25.78082592057721
52-53	25.487921923501393	24.226506359899034	24.567708930381638	25.717862786217932
54-55	25.49047814384212	23.492658421321767	24.780876494023904	26.23598694081221
56-57	25.932436464954638	23.720967871551395	24.436244661977206	25.910351001516762
58-59	26.034698614742613	24.0977781125847	24.806344491211224	25.061178781461468
60-61	25.372807966727358	25.059092219838234	24.190007976395712	25.378091837038696
62-63	24.88994656072466	24.398597429745053	24.930124354486146	25.781331655044138
64-65	25.517506841463483	24.096561567552076	24.339932231581432	26.04599935940301
66-67	25.178664748621877	24.350046920919986	24.181524957995943	26.289763372462193
68-69	25.238847150186277	24.566445080046527	24.85853483105659	25.3361729387106
70-71	25.901584072735854	24.350608848105463	24.504183547895863	25.24362353126282
72-73	25.270820807039822	24.38297585398884	24.90820919425261	25.43799414471873
74-75	25.408942509229654	24.08970605588928	25.085553413988613	25.415798020892456
76-77	25.554060827562335	23.331299154635794	24.448636425959265	26.666003591842607
78-79	25.7066525199416	23.211662506195747	24.518529924482113	26.563155049380537
80-81	24.943073257356083	24.29979544586059	24.486872569514688	26.27025872726864
82-83	24.82802102520935	24.4758588833982	25.00238820664225	25.693731884750203
84-85	25.216726260417666	23.667682092485233	25.170366199525834	25.945225447571268
86-87	25.10994105383824	24.729179192960178	24.47760439202288	25.683275361178698
88-89	25.577801628464986	23.711585252782942	23.963441017312974	26.747172101439094
90-91	25.464011373406237	23.693772161003377	24.121848290898466	26.72036817469192
92-93	25.369944762557665	23.909271236633156	24.753679218246898	25.967104782562277
94-95	25.481037767126136	23.50732472086267	24.63668597823094	26.374951533780255
96-97	25.932433875218447	24.14123477879736	24.45080046527571	25.47553088070848
98-99	24.049078720379413	24.39708022634427	25.144949117493354	26.408891935782965
100-101	25.180167211366957	23.838641255817198	24.462085036224632	26.519106496591217
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	2.5
4	2.0
5	1.0
6	2.5
7	6.5
8	10.5
9	8.5
10	7.5
11	9.0
12	10.5
13	11.5
14	12.0
15	15.0
16	16.5
17	16.5
18	19.0
19	23.0
20	29.0
21	34.0
22	41.0
23	55.0
24	64.0
25	71.5
26	89.5
27	126.0
28	161.0
29	169.5
30	208.5
31	281.0
32	402.0
33	684.5
34	1247.5
35	2352.5
36	4431.5
37	7446.5
38	11004.5
39	14541.0
40	17804.0
41	20279.5
42	21996.0
43	23965.5
44	26003.0
45	28673.0
46	31187.0
47	32458.5
48	34050.5
49	38795.5
50	44703.5
51	45984.0
52	45136.5
53	46658.0
54	50845.5
55	52839.5
56	46163.5
57	39951.5
58	39146.0
59	37859.5
60	32041.0
61	24702.0
62	20157.5
63	14393.5
64	8874.0
65	5763.5
66	4252.0
67	3461.5
68	2530.0
69	1630.0
70	1060.5
71	753.5
72	582.0
73	467.0
74	346.5
75	252.5
76	165.0
77	94.0
78	59.5
79	39.5
80	19.5
81	12.0
82	9.5
83	4.0
84	1.5
85	2.5
86	2.0
87	1.0
88	0.5
89	0.5
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4786495765878657
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.057344669277755E-4
22-23	0.0013486252451407345
24-25	5.619271854753061E-4
26-27	0.0026410577717339385
28-29	0.00370871942413702
30-31	8.428907782129592E-4
32-33	1.1238543709506121E-4
34-35	0.003933490298327143
36-37	0.002022937867711102
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.007754595159559225
46-47	0.07833264965525767
48-49	8.428907782129592E-4
50-51	0.0
52-53	1.1238543709506121E-4
54-55	0.0
56-57	0.0078107878781067544
58-59	0.04422366949690659
60-61	0.03320989666159059
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	1.1238543709506121E-4
78-79	0.008091751470844408
80-81	0.006181199040228368
82-83	6.743126225703673E-4
84-85	0.002303901460448755
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06979135643603301
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	889795.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.03557099336669
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5730301075712	34.33012021707565
2	14.40817364174895	13.26577001138977
3	4.964599781203943	6.856445570438006
4	2.205566154146887	4.06137989079183
5	1.1209306957785816	2.5801342312079405
6	0.6489966927241673	1.7926159993418154
7	0.4152860925036668	1.338255267580728
8	0.26693231243685633	0.9830705135688351
9	0.19498910582100723	0.8078791341560463
>10	0.9474243445019953	7.989405999174207
>50	0.09913226992423674	3.1999303754679818
>100	0.13119620486478908	13.22129188296505
>500	0.01762337080114024	5.435835663135399
>1k	0.006119225972618138	4.1378652437067664
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	2934	0.32973887243690964	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	2028	0.22791766642878417	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2024	0.22746812468040392	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1915	0.21521811203704225	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1802	0.2025185576453003	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1703	0.19139239937288927	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1603	0.18015385566338313	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1520	0.17082586438449304	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1519	0.170713478947398	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	1498	0.1683533847684017	TruSeq Adapter, Index 3 (95% over 21bp)
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1456	0.16363319641040916	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1399	0.15722722649599064	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	1372	0.154192819694424	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	1286	0.14452767210424874	No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT	1251	0.1405941818059216	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1227	0.13789693131564013	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	1224	0.13755977500435493	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1220	0.1371102332559747	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1210	0.13598637888502407	No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA	1155	0.12980517984479573	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	1152	0.12946802353351053	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1104	0.1240735225529476	No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC	1096	0.1231744390561871	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	1016	0.1141836040885822	No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC	1008	0.11328452059182172	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	989	0.11114919728701556	No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT	977	0.10980057204187481	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	973	0.10935103029349456	No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG	922	0.10361937300164645	No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA	907	0.10193359144522053	No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG	893	0.10036019532588968	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.1238543709506122E-4	0.0
3	0.0	0.0	0.0	1.1238543709506122E-4	0.0
4	0.0	0.0	0.0	1.1238543709506122E-4	0.0
5	0.0	0.0	0.0	1.1238543709506122E-4	0.0
6	0.0	0.0	0.0	1.1238543709506122E-4	0.0
7	0.0	0.0	0.0	1.1238543709506122E-4	0.0
8	0.0	0.0	0.0	1.1238543709506122E-4	0.0
9	0.0	0.0	0.0	1.1238543709506122E-4	0.0
10-11	0.0	0.0	0.0	2.2477087419012245E-4	0.0
12-13	0.0	0.0	0.0	2.2477087419012245E-4	0.0
14-15	0.0	0.0	0.0	2.2477087419012245E-4	5.619271854753061E-5
16-17	0.0	0.0	0.0	2.2477087419012245E-4	2.2477087419012245E-4
18-19	0.0	0.0	0.0	2.809635927376531E-4	3.371563112851837E-4
20-21	0.0	0.0	0.0	4.4954174838024495E-4	3.371563112851837E-4
22-23	0.0	0.0	0.0	8.428907782129591E-4	3.371563112851837E-4
24-25	0.0	0.0	0.0	0.0016857815564259183	4.495417483802449E-4
26-27	0.0	0.0	0.0	0.0025286723346388774	4.495417483802449E-4
28-29	0.0	0.0	0.0	0.004214453891064796	4.495417483802449E-4
30-31	0.0	0.0	0.0	0.01208143448771908	4.495417483802449E-4
32-33	0.0	0.0	0.0	0.032760354913210346	4.495417483802449E-4
34-35	0.0	0.0	0.0	0.05658606757736333	4.495417483802449E-4
36-37	0.0	0.0	0.0	0.0956400069678971	4.495417483802449E-4
38-39	0.0	0.0	0.0	0.17813091779567203	4.495417483802449E-4
40-41	0.0	0.0	0.0	0.28096359273765303	4.495417483802449E-4
42-43	0.0	0.0	0.0	0.37722171960957296	5.057344669277755E-4
44-45	0.0	0.0	0.0	0.47904292561769846	5.619271854753062E-4
46-47	0.0	0.0	0.0	0.5876634505700751	6.181199040228367E-4
48-49	0.0	0.0	0.0	0.7033642580594407	7.866980596654286E-4
50-51	0.0	0.0	0.0	0.8324389325631185	7.866980596654286E-4
52-53	0.0	0.0	0.0	0.9561752988047809	8.428907782129591E-4
54-55	0.0	0.0	0.0	1.0787878106754927	8.990834967604898E-4
56-57	0.0	0.0	0.0	1.2193257997628668	8.990834967604898E-4
58-59	0.0	0.0	0.0	1.3676183840098	8.990834967604898E-4
60-61	0.0	0.0	0.0	1.540635764417647	8.990834967604898E-4
62-63	0.0	0.0	0.0	1.7159008535673947	0.0013486252451407347
64-65	0.0	0.0	0.0	1.8795902426963513	0.0013486252451407347
66-67	0.0	0.0	0.0	2.0601374473895673	0.0013486252451407347
68-69	0.0	0.0	0.0	2.2680505060154306	0.0013486252451407347
70-71	0.0	0.0	0.0	2.499789277305447	0.001461010682235796
72-73	0.0	0.0	0.0	2.7458571918250834	0.0017981669935209796
74-75	0.0	0.0	0.0	2.9861934490528714	0.0017981669935209796
76-77	0.0	0.0	0.0	3.2240572266645686	0.0017981669935209796
78-79	0.0	0.0	0.0	3.4890058946161755	0.0017981669935209796
80-81	0.0	0.0	0.0	3.796548643226811	0.0017981669935209796
82-83	0.0	0.0	0.0	4.131794402081378	0.0017981669935209796
84-85	0.0	0.0	0.0	4.46057799830298	0.0017981669935209796
86-87	0.0	0.0	0.0	4.806949915429959	0.0017981669935209796
88-89	0.0	0.0	0.0	5.166527121415607	0.0017981669935209796
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1240	0.0	52.336037	1
GGTATCA	570	0.0	49.392475	1
ATCAACG	1780	0.0	35.751064	3
TCAACGC	1850	0.0	34.398323	4
TAATACC	115	1.0378244E-7	33.036674	4
TAGCGTT	90	2.4157513E-5	31.66015	6
AATGCGG	225	0.0	31.660149	7
AAACCGT	160	1.9081199E-9	29.68139	6
GTACATG	4480	0.0	29.07825	1
TACATGG	4505	0.0	28.884176	2
AAATGCG	260	0.0	27.398205	6
ACATGGG	4700	0.0	27.180573	3
CGCAGAG	2425	0.0	26.24202	8
TATCAAC	2460	0.0	26.061707	2
ACGCAGA	2480	0.0	25.66004	7
CAACGCA	2530	0.0	25.152924	5
AACCGTG	190	1.19289325E-8	24.994854	7
GTATAGT	115	1.2421765E-4	24.896427	1
ACCGTGC	210	1.2678356E-9	24.87583	8
ATAGCGT	115	1.2832278E-4	24.777506	5
>>END_MODULE