FastQCFastQC Report
Fri 10 Feb 2017
SRR3224537_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224537_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729821
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG21280.2915783459231784No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA14160.19402017755038564No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA13770.18867640147378603No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA10560.1446930137663893No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA9510.13030592432939037No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT9460.1296208248323904No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA8990.1231808895605909No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG8790.1204404915725911No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC8280.11345247670319161No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC8130.11139717821219176No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC7830.10728658123019207No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA7750.10619042203499215No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA7610.10427214344339228No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG7420.10166876535479248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6150.046.4006541
GTATCAA11850.046.1559261
AAACCGT1003.039895E-837.9833686
ATCAACG14900.036.326373
TCAACGC15700.034.7777634
CCGTGCG1151.03325874E-733.051669
TAGGGTA1151.0394979E-733.0290154
TCGCACC7900.031.3057561
ACCGTGC1401.7102138E-830.5244378
AAGGACG804.5035844E-429.6745035
TATCAAC18800.029.043132
CCTCGTC1153.873147E-628.9023698
CGCAGAG19450.028.5627148
ATACCGT1503.3473043E-828.4875246
CGCACCA8850.027.8973852
ACGCAGA20150.027.3329457
CGGTCTA651.0057596E-525.56572728-29
CTATTAA3350.025.5549851
CTGTGCG9350.025.4073589
AAATGCG1701.1264092E-725.1360516
Produced by FastQC (version 0.11.5)