##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224537_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	729821
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12748057400376	34.0	31.0	34.0	31.0	34.0
2	32.290484927125966	34.0	31.0	34.0	31.0	34.0
3	32.43538484094045	34.0	31.0	34.0	31.0	34.0
4	35.664276582888135	37.0	37.0	37.0	35.0	37.0
5	35.672958163714114	37.0	37.0	37.0	35.0	37.0
6	35.78149299622784	37.0	37.0	37.0	35.0	37.0
7	35.74956598946865	37.0	37.0	37.0	35.0	37.0
8	35.784351231329325	37.0	37.0	37.0	35.0	37.0
9	37.527544425276886	39.0	39.0	39.0	35.0	39.0
10-11	37.60193047336264	39.0	39.0	39.0	36.0	39.0
12-13	37.602779996738924	39.0	39.0	39.0	35.0	39.0
14-15	39.10436942757197	41.0	40.0	41.0	37.0	41.0
16-17	38.94845242874622	41.0	40.0	41.0	36.5	41.0
18-19	39.03747151698842	41.0	40.0	41.0	36.0	41.0
20-21	39.10198391112341	41.0	40.0	41.0	36.0	41.0
22-23	39.063502557476426	41.0	40.0	41.0	36.0	41.0
24-25	39.00829518470968	41.0	40.0	41.0	36.0	41.0
26-27	38.857323919152776	41.0	39.5	41.0	35.0	41.0
28-29	38.79842934089318	41.0	39.0	41.0	35.0	41.0
30-31	38.73506928411213	41.0	39.0	41.0	35.0	41.0
32-33	38.55861574276432	41.0	39.0	41.0	35.0	41.0
34-35	38.56846815863068	41.0	39.0	41.0	35.0	41.0
36-37	38.568321547338314	41.0	39.0	41.0	35.0	41.0
38-39	38.44660128990533	41.0	39.0	41.0	35.0	41.0
40-41	38.36301709597285	41.0	39.0	41.0	35.0	41.0
42-43	38.234870605258	40.0	38.0	41.0	34.0	41.0
44-45	38.07292267556017	40.0	38.0	41.0	34.0	41.0
46-47	37.920570249417324	40.0	38.0	41.0	33.0	41.0
48-49	37.733978605712906	40.0	37.5	41.0	33.0	41.0
50-51	36.48325411299483	39.0	35.5	40.0	31.5	40.5
52-53	36.78644146989467	39.0	36.0	40.0	31.5	40.5
54-55	37.24263689315599	40.0	36.0	41.0	33.0	41.0
56-57	37.11977183446352	40.0	35.0	41.0	32.5	41.0
58-59	36.99859075033467	40.0	35.0	41.0	32.5	41.0
60-61	36.78991218394647	39.0	35.0	41.0	32.0	41.0
62-63	36.544142330790706	39.0	35.0	41.0	31.5	41.0
64-65	36.338287059429646	39.0	35.0	41.0	31.0	41.0
66-67	36.149356486042464	38.0	35.0	41.0	31.0	41.0
68-69	35.895991619862954	37.0	35.0	40.5	31.0	41.0
70-71	35.63161240907017	37.0	35.0	40.0	30.5	41.0
72-73	35.341374117763124	36.0	35.0	39.5	30.0	41.0
74-75	35.05285611129304	36.0	35.0	39.0	30.0	41.0
76-77	34.709478077501196	35.0	35.0	39.0	30.0	40.5
78-79	34.43640837958897	35.0	35.0	37.5	29.5	39.0
80-81	34.09378327014432	35.0	34.0	37.0	29.0	39.0
82-83	33.7987903883281	35.0	34.0	37.0	29.0	39.0
84-85	33.5292996501882	35.0	34.0	36.0	29.0	37.0
86-87	33.26194094168296	35.0	34.0	36.0	29.0	37.0
88-89	33.07983532948491	35.0	34.0	35.5	28.5	37.0
90-91	32.87072172491611	35.0	34.0	35.0	27.0	36.0
92-93	32.70324093168051	35.0	34.0	35.0	27.0	36.0
94-95	32.545389211875246	35.0	34.0	35.0	27.0	36.0
96-97	32.386631790534935	35.0	33.0	35.0	26.0	36.0
98-99	32.324598771479586	35.0	33.0	35.0	25.0	35.0
100-101	31.111386901719733	34.5	31.0	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	8.0
7	683.0
8	3261.0
9	2175.0
10	1364.0
11	1365.0
12	1493.0
13	1498.0
14	1412.0
15	1333.0
16	1411.0
17	1498.0
18	1617.0
19	1762.0
20	1986.0
21	2620.0
22	3204.0
23	3159.0
24	3239.0
25	3306.0
26	3401.0
27	3577.0
28	3920.0
29	4386.0
30	5551.0
31	6975.0
32	9608.0
33	13841.0
34	23465.0
35	43066.0
36	93538.0
37	154766.0
38	231369.0
39	93919.0
40	45.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.31370913072952	19.22752859099305	13.579864036688688	24.878898241588747
2	15.619857471900644	21.231507451827227	38.139351978087774	25.009283098184348
3	20.275547395248143	26.28401523663369	28.271902660930092	25.168534707188073
4	13.663487348267589	17.782305524231283	37.742679369324804	30.81152775817632
5	13.605390910921994	38.499714313509756	34.18838317888907	13.706511596679185
6	28.469172577933495	37.16226307546645	19.818969308912735	14.549595037687324
7	26.095028781029868	31.022949462950507	23.25172884858068	19.63029290743895
8	24.471205953240588	35.35716292077098	21.77273605445719	18.39889507153124
9	26.553086304724033	15.93911383750262	20.2775749122045	37.23022494556885
10-11	25.36813821471292	25.894294628408883	27.71946819836645	21.018098958511743
12-13	25.964380306951977	23.461711844411166	28.187048605068917	22.386859243567944
14-15	22.949264729567005	25.45919706563463	25.34076976375535	26.25076844104301
16-17	22.670271967574234	28.200944334711604	26.729183535091693	22.39960016262247
18-19	22.52466664565142	27.767399596233155	27.138107175016344	22.569826583099083
20-21	23.338316037797274	26.50819336183139	26.95087958098677	23.20261101938457
22-23	23.82633267909648	26.839295617938475	26.40802030853563	22.92635139442941
24-25	23.050447478722404	26.81603865700881	26.67529578956817	23.458218074700614
26-27	22.535320304567833	27.988438260888625	27.098288484436594	22.377952950106945
28-29	22.370279835740543	27.792568314696343	27.207630364157787	22.629521485405327
30-31	22.39836891511754	27.334236751203377	27.178102575837087	23.08929175784199
32-33	22.386722223668542	27.317246283677775	27.680554546936854	22.615476945716825
34-35	22.85560431941531	26.87254820017511	27.20372529702489	23.06812218338469
36-37	22.04144535910243	26.96319310582539	27.941114999530605	23.05424653554158
38-39	22.480143219576632	27.19404320566849	27.11539028088275	23.21042329387213
40-41	22.6007110425358	26.95857002206849	27.721683508650287	22.71903542674542
42-43	22.66635242144669	27.30199468066885	27.578512204179006	22.45314069370546
44-45	22.727173064009975	26.70186233264358	27.652213832513397	22.91875077083305
46-47	22.81264858095341	26.378385932989048	27.330057644271676	23.478907841785862
48-49	22.107270138842264	26.9845619679346	27.44570243936527	23.462465453857863
50-51	22.234972685083054	27.26586382140278	27.218112386461886	23.281051107052278
52-53	22.354728077158644	27.084723514395996	26.90961208296281	23.65093632548255
54-55	22.781271023990815	26.576859257269938	26.979629251556204	23.66224046718305
56-57	22.484965491538336	26.820754678201915	27.078900168671495	23.61537966158825
58-59	22.58884118289546	27.15653397761549	27.169073360184516	23.085551479304538
60-61	22.38576881937416	27.39266555067299	26.956660570499807	23.264905059453046
62-63	22.092182566904214	26.909505329242545	27.636061356361267	23.36225074749197
64-65	22.95970088384574	26.273008605415484	26.99530146370886	23.771989047029916
66-67	22.321602324274693	26.82646055174115	26.775000342611243	24.07693678137291
68-69	22.30958002030635	26.858503660486612	28.028927641161328	22.802988678045715
70-71	22.9941314377087	26.50958248666454	27.41836696943497	23.077919106191793
72-73	22.455711743016437	26.656673348670427	28.039341153515725	22.84827375479741
74-75	22.645141753936922	26.563842366826933	27.74385774045965	23.047158138776496
76-77	22.54210278958813	26.260274779706254	27.166455884388093	24.03116654631752
78-79	22.746950801699327	25.612100863368507	27.601411538988625	24.03953679594354
80-81	22.53962639488406	26.558373143096475	27.133772457470563	23.768228004548902
82-83	22.052615308901256	26.86066285610883	27.591541466377045	23.495180368612868
84-85	22.537357926802105	25.817659737046796	28.001260969233037	23.64372136691806
86-87	22.278212538662338	26.95320211902516	27.25962171233027	23.508963629982233
88-89	22.643064992098203	26.116976730479553	26.86596827494137	24.373990002480873
90-91	22.496543673037635	26.068926490194173	27.219551095405585	24.214978741362607
92-93	22.38098761575121	26.239178512410284	27.766244906124708	23.6135889657138
94-95	22.713788723536318	25.82681232795439	27.73419783755195	23.725201110957343
96-97	23.103610337329293	26.186558073829065	27.523735272073562	23.186096316768083
98-99	22.119844398496603	26.22575920132966	27.702096290419604	23.952300109754145
100-101	22.816176803214304	25.94816371460022	27.3363957509983	23.899263731187173
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	1.5
2	1.5
3	2.5
4	1.5
5	1.5
6	3.5
7	6.0
8	7.0
9	9.0
10	10.5
11	12.5
12	16.0
13	19.5
14	26.5
15	25.0
16	17.5
17	25.5
18	37.5
19	41.5
20	46.5
21	50.5
22	55.5
23	72.0
24	101.0
25	130.0
26	161.5
27	191.5
28	223.0
29	269.0
30	414.5
31	787.0
32	1770.0
33	3888.0
34	6886.0
35	12154.5
36	20688.0
37	29850.0
38	36216.0
39	41673.0
40	47122.0
41	46735.5
42	41152.5
43	35577.5
44	30399.0
45	26893.0
46	25130.5
47	22055.0
48	20201.0
49	21034.5
50	22056.5
51	21511.0
52	20690.0
53	21389.5
54	23232.0
55	23961.5
56	20294.0
57	17165.0
58	16936.5
59	16845.0
60	14424.0
61	10852.0
62	8746.0
63	6125.5
64	3635.0
65	2295.5
66	1834.0
67	1714.0
68	1351.0
69	812.0
70	453.0
71	303.5
72	240.0
73	192.5
74	149.0
75	112.0
76	84.0
77	50.5
78	35.0
79	25.0
80	16.5
81	13.0
82	10.5
83	7.0
84	7.5
85	6.5
86	6.0
87	4.5
88	1.0
89	2.5
90	5.0
91	4.0
92	1.5
93	1.5
94	3.5
95	5.5
96	5.5
97	4.0
98	2.0
99	2.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1500367898429889
2	0.0
3	1.3701989939998987E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03555666389429737
16-17	0.07186693723529468
18-19	0.02630782068479805
20-21	0.040763420071496984
22-23	0.06474190246649522
24-25	0.01664791777709877
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.022128713753098363
38-39	0.00445314673049967
40-41	0.006508445221499519
42-43	0.004110596981999696
44-45	0.012468810845399077
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.016442387927998785
60-61	0.01747003717349871
62-63	0.029390768421297824
64-65	0.04638123594689657
66-67	0.01794960682139867
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.016579407827398772
80-81	0.05679474830129579
82-83	0.048847594136096385
84-85	0.030623947515897736
86-87	0.05878153684259565
88-89	0.03288477585599757
90-91	0.0
92-93	0.0031514576861997666
94-95	0.0
96-97	0.0
98-99	0.001096159195199919
100-101	0.04549060660079663
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	729821.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.83317443291344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.43256192357437	24.83755315789252
2	14.559673840132636	10.72558012479864
3	6.278195773921339	6.9373764019447375
4	3.3294175949410296	4.905320761378962
5	2.029608929631948	3.7378469867866135
6	1.273937047892567	2.815388730094663
7	0.9159221348706836	2.3615423832460842
8	0.6590553415880536	1.9420080286124901
9	0.5091597139871961	1.6878571703552436
>10	2.5895886032211832	17.159105969764187
>50	0.21900447216013932	5.654303512999901
>100	0.18950127395826358	13.37054323305745
>500	0.012860365897367164	3.032857722626105
>1k	0.0015129842232196665	0.8327158164424746
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2128	0.2915783459231784	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1416	0.19402017755038564	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1377	0.18867640147378603	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1056	0.1446930137663893	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	951	0.13030592432939037	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	946	0.1296208248323904	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	899	0.1231808895605909	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	879	0.1204404915725911	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	828	0.11345247670319161	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	813	0.11139717821219176	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	783	0.10728658123019207	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	775	0.10619042203499215	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	761	0.10427214344339228	No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG	742	0.10166876535479248	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.110596981999696E-4	0.0
10-11	0.0	0.0	0.0	5.480795975999595E-4	0.0
12-13	0.0	0.0	0.0	5.480795975999595E-4	0.0
14-15	0.0	0.0	0.0	8.221193963999392E-4	0.0
16-17	0.0	0.0	0.0	8.906293460999341E-4	0.0
18-19	0.0	0.0	0.0	0.001096159195199919	0.0
20-21	0.0	0.0	0.0	0.0014387089436998935	0.0
22-23	0.0	0.0	0.0	0.002808907937699792	6.850994969999493E-5
24-25	0.0	0.0	0.0	0.005343776076599605	1.3701989939998987E-4
26-27	0.0	0.0	0.0	0.012126261096899103	1.3701989939998987E-4
28-29	0.0	0.0	0.0	0.02363593264649825	1.3701989939998987E-4
30-31	0.0	0.0	0.0	0.05220458167139613	1.3701989939998987E-4
32-33	0.0	0.0	0.0	0.10756062102899204	1.3701989939998987E-4
34-35	0.0	0.0	0.0	0.1782628891193868	1.3701989939998987E-4
36-37	0.0	0.0	0.0	0.28445331115437894	1.3701989939998987E-4
38-39	0.0	0.0	0.0	0.44168364571586727	2.7403979879997973E-4
40-41	0.0	0.0	0.0	0.6137121294125546	4.110596981999696E-4
42-43	0.0	0.0	0.0	0.7817670360266422	4.7956964789996453E-4
44-45	0.0	0.0	0.0	0.9753076439291277	5.480795975999595E-4
46-47	0.0	0.0	0.0	1.1997462391463112	5.480795975999595E-4
48-49	0.0	0.0	0.0	1.4265826826029944	5.480795975999595E-4
50-51	0.0	0.0	0.0	1.6500621385243779	5.480795975999595E-4
52-53	0.0	0.0	0.0	1.8706641765583616	6.850994969999493E-4
54-55	0.0	0.0	0.0	2.0874296574091455	8.221193963999392E-4
56-57	0.0	0.0	0.0	2.3382445832608267	8.221193963999392E-4
58-59	0.0	0.0	0.0	2.6076257054812073	9.591392957999291E-4
60-61	0.0	0.0	0.0	2.8827616634763866	0.001096159195199919
62-63	0.0	0.0	0.0	3.172764280556465	0.001096159195199919
64-65	0.0	0.0	0.0	3.4635890170329438	0.001096159195199919
66-67	0.0	0.0	0.0	3.8027132680479188	0.001096159195199919
68-69	0.0	0.0	0.0	4.18760216546249	0.001096159195199919
70-71	0.0	0.0	0.0	4.576122090211161	0.0015072188933998884
72-73	0.0	0.0	0.0	4.985880099366831	0.0015072188933998884
74-75	0.0	0.0	0.0	5.3915960214902015	0.0015072188933998884
76-77	0.0	0.0	0.0	5.80765694601827	0.0015072188933998884
78-79	0.0	0.0	0.0	6.272017385084836	0.0015072188933998884
80-81	0.0	0.0	0.0	6.783581179494698	0.0016442387927998784
82-83	0.0	0.0	0.0	7.304051267365559	0.0019182785915998581
84-85	0.0	0.0	0.0	7.83287956909982	0.0019182785915998581
86-87	0.0	0.0	0.0	8.38150724629738	0.0019182785915998581
88-89	0.0	0.0	0.0	8.973090661956835	0.0019182785915998581
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	615	0.0	46.400654	1
GTATCAA	1185	0.0	46.155926	1
AAACCGT	100	3.039895E-8	37.983368	6
ATCAACG	1490	0.0	36.32637	3
TCAACGC	1570	0.0	34.777763	4
CCGTGCG	115	1.03325874E-7	33.05166	9
TAGGGTA	115	1.0394979E-7	33.029015	4
TCGCACC	790	0.0	31.305756	1
ACCGTGC	140	1.7102138E-8	30.524437	8
AAGGACG	80	4.5035844E-4	29.674503	5
TATCAAC	1880	0.0	29.04313	2
CCTCGTC	115	3.873147E-6	28.902369	8
CGCAGAG	1945	0.0	28.562714	8
ATACCGT	150	3.3473043E-8	28.487524	6
CGCACCA	885	0.0	27.897385	2
ACGCAGA	2015	0.0	27.332945	7
CGGTCTA	65	1.0057596E-5	25.565727	28-29
CTATTAA	335	0.0	25.554985	1
CTGTGCG	935	0.0	25.407358	9
AAATGCG	170	1.1264092E-7	25.136051	6
>>END_MODULE