FastQCFastQC Report
Fri 10 Feb 2017
SRR3224537_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224537_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729821
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT51190.7014048650285482No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT22350.30623947515897737TruSeq Adapter, Index 3 (95% over 21bp)
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG16770.22978237129378298No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT12970.17771480952178684No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA11720.1605873220967881No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA11450.15688778481298837No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA11040.15126996893758882No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT10540.1444189739675893No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG10370.14208963567778948TruSeq Adapter, Index 3 (96% over 28bp)
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG9990.13688287950058986No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG9570.1311280437257903No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG9490.1300318845305904No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG9020.12359194925879087No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC8640.11838519308159125No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG8440.11564479509359143No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG8430.11550777519419146No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA8260.11317843690439164No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT8110.11112313841339176No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC8060.11043803891639181No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7250.048.7250671
GTATCAA12100.042.214061
CCGTGCG801.07880605E-535.6166279
AGCCGTA300.003955848531.65922462-63
GACCGTG600.003954563831.6592247
ATCAACG16150.031.1691423
TCAACGC16500.030.5079774
ACCGTGC953.4947207E-529.9929478
TCGCACC7100.028.911381
CGCACCA7350.027.7825832
CCTCGTC1257.3995634E-626.5937468
CGCAGAG18950.026.5636738
TAGTCGT458.914501E-426.40620442-43
AAACCGT908.95669E-426.3826876
CCTATCC908.95669E-426.3826873
TATCAAC19700.025.7934252
CTCGTCC1301.0032822E-525.570919
GTCTAGT1301.0032822E-525.570919
AATGCGG1301.0032822E-525.570917
GCGAATT751.0626154E-625.32737714-15
Produced by FastQC (version 0.11.5)