##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224537_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	729821
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.163964862617	34.0	33.0	34.0	31.0	34.0
2	33.29733044129999	34.0	33.0	34.0	31.0	34.0
3	33.42368608192968	34.0	34.0	34.0	31.0	34.0
4	36.62521357976819	37.0	37.0	37.0	35.0	37.0
5	36.6593068711369	37.0	37.0	37.0	35.0	37.0
6	36.70569632827775	37.0	37.0	37.0	36.0	37.0
7	36.69363994732955	37.0	37.0	37.0	36.0	37.0
8	36.683477181391055	37.0	37.0	37.0	36.0	37.0
9	38.5467916105456	39.0	39.0	39.0	38.0	39.0
10-11	38.572166325715486	39.0	39.0	39.0	37.5	39.0
12-13	38.561906960747905	39.0	39.0	39.0	38.0	39.0
14-15	40.171909961483706	41.0	40.0	41.0	38.0	41.0
16-17	40.18160617466475	41.0	40.0	41.0	38.0	41.0
18-19	40.18295924617132	41.0	40.0	41.0	38.5	41.0
20-21	40.19014731009385	41.0	40.0	41.0	39.0	41.0
22-23	40.117775454529266	41.0	40.0	41.0	38.0	41.0
24-25	40.08359858102192	41.0	40.0	41.0	38.0	41.0
26-27	40.0250075018395	41.0	40.0	41.0	38.0	41.0
28-29	40.01050737098549	41.0	40.0	41.0	38.0	41.0
30-31	39.94547224593428	41.0	40.0	41.0	38.0	41.0
32-33	39.92070110342125	41.0	40.0	41.0	38.0	41.0
34-35	39.85021875226939	41.0	40.0	41.0	38.0	41.0
36-37	39.740593926455944	41.0	40.0	41.0	37.0	41.0
38-39	39.63547294473577	41.0	40.0	41.0	37.0	41.0
40-41	39.528492602980734	41.0	40.0	41.0	37.0	41.0
42-43	39.46161044968561	41.0	39.0	41.0	36.0	41.0
44-45	39.25057377082874	41.0	39.0	41.0	35.0	41.0
46-47	39.058935684229425	41.0	39.0	41.0	35.0	41.0
48-49	38.98204011668615	41.0	39.0	41.0	35.0	41.0
50-51	38.823225832087594	40.0	38.0	41.0	35.0	41.0
52-53	38.5998155712154	40.0	37.5	41.0	35.0	41.0
54-55	38.42935528026736	40.0	37.0	41.0	35.0	41.0
56-57	38.20849084912602	40.0	36.5	41.0	35.0	41.0
58-59	37.95941128030024	40.0	35.5	41.0	34.5	41.0
60-61	37.73249879079939	39.5	35.0	41.0	34.0	41.0
62-63	37.521638867612744	39.0	35.0	41.0	34.0	41.0
64-65	37.28237266398199	39.0	35.0	41.0	34.0	41.0
66-67	36.999205969682976	38.5	35.0	41.0	33.0	41.0
68-69	36.715981041926725	37.5	35.0	41.0	33.0	41.0
70-71	36.370432612928376	37.0	35.0	40.0	33.0	41.0
72-73	36.063020247430536	36.5	35.0	39.5	33.0	41.0
74-75	35.755563350465394	36.0	35.0	39.0	33.0	41.0
76-77	33.88731620493244	34.5	33.0	37.0	29.5	39.0
78-79	35.14296930343194	35.0	35.0	38.0	32.5	39.0
80-81	35.01108079926448	35.0	35.0	37.0	33.0	39.0
82-83	34.764354547210885	35.0	35.0	37.0	33.0	39.0
84-85	34.51667395155799	35.0	35.0	36.5	33.0	38.0
86-87	34.27970899713766	35.0	35.0	36.0	32.5	37.0
88-89	34.10065139260175	35.0	35.0	36.0	32.0	37.0
90-91	33.92047502058724	35.0	35.0	35.5	32.0	36.5
92-93	33.80428625649303	35.0	35.0	35.0	32.0	36.0
94-95	33.68522281490941	35.0	35.0	35.0	32.0	36.0
96-97	33.616546386031644	35.0	35.0	35.0	32.0	36.0
98-99	33.571727862037406	35.0	34.0	35.0	31.5	36.0
100-101	32.7609276795269	34.5	33.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	9.0
15	24.0
16	88.0
17	132.0
18	244.0
19	401.0
20	549.0
21	715.0
22	1083.0
23	1226.0
24	1452.0
25	2232.0
26	3227.0
27	5049.0
28	7317.0
29	6825.0
30	5022.0
31	5161.0
32	6005.0
33	8150.0
34	13680.0
35	29973.0
36	84714.0
37	169444.0
38	252159.0
39	124844.0
40	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.89170398741122	20.388071496129335	14.054444002362462	23.665780514096983
2	14.8481613984799	21.48883082290041	40.45361807895361	23.209389699666083
3	19.22622122410838	27.906568870997134	29.583966479451814	23.283243425442677
4	12.991267721811239	18.596203726667223	39.398838893372485	29.013689658149055
5	13.007436069940436	38.52629617399335	35.56036343158117	12.905904324485046
6	27.30217409474378	37.7826891799496	20.81989967402966	14.095237051276957
7	25.497622019645917	31.47360791207707	23.759524595757043	19.269245472519973
8	23.812003217227236	35.89661026470875	22.446051840108737	17.84533467795528
9	26.196560526485264	16.08545109006181	20.720697266864065	36.99729111658886
10-11	25.026547605508746	25.988358789346975	28.69600902139018	20.2890845837541
12-13	25.726787801392398	23.703414946952748	28.651888613783377	21.917908637871477
14-15	22.84128573992801	25.80852017138449	25.577710150845206	25.772483937842296
16-17	22.480649364707237	28.755955227377672	26.943044938416406	21.82035046949869
18-19	22.460096379797235	27.822849712463743	27.55442772953916	22.162626178199858
20-21	23.01225446705366	26.657422321120887	27.414531480901683	22.91579173092377
22-23	23.695264194570523	27.174651023379294	26.76221632268562	22.367868459364562
24-25	23.46960237374087	27.16415108687982	26.795223743985662	22.57102279539365
26-27	22.855868545887535	28.261825745472084	27.2388826619677	21.643423046672677
28-29	22.676256469211552	27.534211287155575	27.609574090542242	22.179958153090627
30-31	22.410877855012174	27.299390391785295	27.855902813460435	22.4338289397421
32-33	22.84667647274429	27.38540145885387	28.09975617258788	21.668165895813964
34-35	23.49677955964135	26.884322440039384	27.49497630487612	22.12392169544315
36-37	21.994186130159157	27.307580845059615	27.890269544050373	22.80796348073085
38-39	22.39138090024814	26.947498085146904	27.481944202756566	23.179176811848386
40-41	22.27868203299165	26.858503660486612	28.399977528736496	22.46283677778524
42-43	22.736671046736117	27.0356703904108	28.3492801659585	21.878378396894583
44-45	22.628838996475494	26.55005563564409	28.26249609454223	22.55860927333819
46-47	23.096752961825363	25.90603060552874	27.635613207547173	23.36160322509873
48-49	22.25742140131403	26.288100409007765	27.927625494132073	23.526852695546133
50-51	22.233122916441157	26.58549151093213	27.927669935504735	23.25371563712198
52-53	22.34253330611205	26.79102136003212	27.85409024952694	23.0123550843289
54-55	22.667270467690024	26.268975543318156	27.663221529662753	23.400532459329067
56-57	22.95971431821016	26.50072694833802	27.161763476635564	23.377795256816253
58-59	22.440998577866115	27.179959906104894	27.741548583863064	22.637492932165927
60-61	22.537953417675023	27.845435550289434	26.89705411715687	22.719556914878673
62-63	21.88413323266938	27.695078656273253	27.46015803875197	22.960630072305403
64-65	22.40241100214984	27.483862481348165	26.894950953727008	23.218775562774983
66-67	22.17262863085606	27.711658064100646	26.679418651970828	23.436294653072466
68-69	22.41577044234134	27.513184739819764	27.441934392131767	22.629110425707125
70-71	22.933637152123605	27.29813200771148	27.115415971861594	22.652814868303324
72-73	22.399602094212142	27.093766827756394	27.55908640611876	22.947544671912702
74-75	22.55553073972933	26.949895933386404	27.375753780721574	23.11881954616269
76-77	22.556224595567254	26.404441652273817	27.242663758180974	23.796669993977954
78-79	22.776740910050126	26.209503920456257	27.027304792521395	23.98645037697222
80-81	22.261008857512444	27.10550908772007	26.969234395211682	23.66424765955581
82-83	21.976810625129314	27.062400574390566	27.531696301949665	23.429092498530455
84-85	22.649328761351452	26.169161045892235	27.605019131757345	23.57649106099896
86-87	22.058628074555266	27.14275144179189	27.181802113120888	23.616818370531952
88-89	22.683986895416822	26.327071980663753	26.51437818314354	24.47456294077589
90-91	22.736876576585217	26.350707913310252	26.72778667645902	24.18462883364551
92-93	22.461466578791235	26.51889983982374	27.343485594412876	23.676147986972147
94-95	22.509355033631532	26.09509729097957	27.230101627659387	24.16544604772951
96-97	22.9403511271942	26.434153032044843	27.102262061519195	23.52323377924176
98-99	21.50328642228711	26.75923274337132	27.593341380968756	24.144139453372812
100-101	22.300908009611398	26.352776644717675	27.149247438617657	24.19706790705327
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	1.0
4	0.5
5	1.0
6	1.5
7	2.5
8	10.0
9	15.5
10	16.5
11	12.5
12	9.5
13	13.5
14	15.0
15	17.0
16	21.5
17	23.0
18	26.0
19	32.5
20	33.5
21	41.5
22	55.5
23	62.0
24	73.0
25	104.5
26	135.0
27	165.0
28	206.5
29	261.5
30	443.5
31	942.5
32	2242.5
33	4785.5
34	8535.5
35	14794.0
36	23978.0
37	33110.5
38	38922.0
39	43223.0
40	47481.5
41	46316.5
42	39889.0
43	34473.5
44	30142.0
45	26739.0
46	24173.5
47	21376.0
48	20131.5
49	21170.0
50	22827.5
51	21700.0
52	19626.5
53	20031.5
54	22401.0
55	23136.0
56	19167.0
57	16012.0
58	16028.0
59	16371.5
60	13953.0
61	10125.5
62	8113.5
63	5566.0
64	3074.0
65	1801.0
66	1422.5
67	1406.0
68	1072.5
69	601.0
70	327.5
71	215.0
72	152.5
73	133.0
74	118.0
75	79.5
76	52.5
77	35.5
78	24.5
79	7.5
80	2.0
81	2.5
82	1.5
83	0.5
84	0.5
85	1.0
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4740888519239649
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.536094466999443E-4
22-23	0.0011646691448999138
24-25	3.4254974849997466E-4
26-27	0.002055298490999848
28-29	0.002466358189199818
30-31	0.001096159195199919
32-33	2.0552984909998477E-4
34-35	0.004042087032299701
36-37	0.0019867885412998528
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.01000245265619926
46-47	0.07412776557539451
48-49	8.221193963999391E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.007947154165199411
58-59	0.03884514147989713
60-61	0.028705668924297878
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	3.4254974849997466E-4
78-79	0.008084174064599402
80-81	0.006165895472999543
82-83	5.480795975999595E-4
84-85	0.0018497686418998632
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06556402186289514
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	729821.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.68846760339286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.34727122797646	21.677609621304185
2	15.88677958433076	10.704025186979397
3	6.797868860352013	6.870293546222459
4	3.6253491249025327	4.885298261810704
5	2.200723899879866	3.7069507902557626
6	1.490321964488088	3.0123997931569053
7	1.0250210109904634	2.417197098508445
8	0.7452677957227481	2.0085543993646247
9	0.532373187778319	1.6141353200446393
>10	2.859752341421147	17.335899147679296
>50	0.24586072142908613	5.832266128918672
>100	0.2250319877204465	14.506093693457666
>500	0.014702634406417555	3.2499164316026627
>1k	0.0032672520903150124	1.4750583829309574
>5k	4.0840651128937655E-4	0.7043021977636014
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	5119	0.7014048650285482	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	2235	0.30623947515897737	TruSeq Adapter, Index 3 (95% over 21bp)
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1677	0.22978237129378298	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1297	0.17771480952178684	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1172	0.1605873220967881	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1145	0.15688778481298837	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1104	0.15126996893758882	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1054	0.1444189739675893	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG	1037	0.14208963567778948	TruSeq Adapter, Index 3 (96% over 28bp)
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	999	0.13688287950058986	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	957	0.1311280437257903	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	949	0.1300318845305904	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	902	0.12359194925879087	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	864	0.11838519308159125	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	844	0.11564479509359143	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	843	0.11550777519419146	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	826	0.11317843690439164	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	811	0.11112313841339176	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	806	0.11043803891639181	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.110596981999696E-4	1.3701989939998987E-4
10-11	0.0	0.0	0.0	5.480795975999595E-4	1.3701989939998987E-4
12-13	0.0	0.0	0.0	6.165895472999543E-4	1.3701989939998987E-4
14-15	0.0	0.0	0.0	9.591392957999291E-4	2.055298490999848E-4
16-17	0.0	0.0	0.0	9.591392957999291E-4	2.7403979879997973E-4
18-19	0.0	0.0	0.0	0.001096159195199919	2.7403979879997973E-4
20-21	0.0	0.0	0.0	0.0014387089436998935	2.7403979879997973E-4
22-23	0.0	0.0	0.0	0.0026718880382998024	3.4254974849997466E-4
24-25	0.0	0.0	0.0	0.00513824622749962	4.110596981999696E-4
26-27	0.0	0.0	0.0	0.012194771046599098	4.110596981999696E-4
28-29	0.0	0.0	0.0	0.023841462495598237	4.110596981999696E-4
30-31	0.0	0.0	0.0	0.0526841513192961	4.110596981999696E-4
32-33	0.0	0.0	0.0	0.10865678022419195	4.110596981999696E-4
34-35	0.0	0.0	0.0	0.17881096871698676	5.480795975999595E-4
36-37	0.0	0.0	0.0	0.28534394050047884	5.480795975999595E-4
38-39	0.0	0.0	0.0	0.4418891755649673	5.480795975999595E-4
40-41	0.0	0.0	0.0	0.6150823284065545	5.480795975999595E-4
42-43	0.0	0.0	0.0	0.783890844467342	5.480795975999595E-4
44-45	0.0	0.0	0.0	0.9786646314644276	6.850994969999493E-4
46-47	0.0	0.0	0.0	1.2054325649714106	8.221193963999392E-4
48-49	0.0	0.0	0.0	1.433091127824494	8.221193963999392E-4
50-51	0.0	0.0	0.0	1.6593109817338774	8.221193963999392E-4
52-53	0.0	0.0	0.0	1.8828589476049606	8.221193963999392E-4
54-55	0.0	0.0	0.0	2.100309527952745	8.221193963999392E-4
56-57	0.0	0.0	0.0	2.3520150831505258	8.221193963999392E-4
58-59	0.0	0.0	0.0	2.623999583459506	8.221193963999392E-4
60-61	0.0	0.0	0.0	2.9024240190402852	8.221193963999392E-4
62-63	0.0	0.0	0.0	3.1958521336053636	8.221193963999392E-4
64-65	0.0	0.0	0.0	3.4918151163093416	8.221193963999392E-4
66-67	0.0	0.0	0.0	3.834227844909916	8.906293460999341E-4
68-69	0.0	0.0	0.0	4.222542239809488	9.591392957999291E-4
70-71	0.0	0.0	0.0	4.616268920735358	0.001096159195199919
72-73	0.0	0.0	0.0	5.034248123855027	0.0012331790945999087
74-75	0.0	0.0	0.0	5.444691232507697	0.0012331790945999087
76-77	0.0	0.0	0.0	5.865136793816566	0.0012331790945999087
78-79	0.0	0.0	0.0	6.336690777601631	0.0012331790945999087
80-81	0.0	0.0	0.0	6.856886825673692	0.0012331790945999087
82-83	0.0	0.0	0.0	7.382974729419954	0.0012331790945999087
84-85	0.0	0.0	0.0	7.920435284816414	0.0012331790945999087
86-87	0.0	0.0	0.0	8.475776937084573	0.0012331790945999087
88-89	0.0	0.0	0.0	9.073594758166728	0.0012331790945999087
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	725	0.0	48.725067	1
GTATCAA	1210	0.0	42.21406	1
CCGTGCG	80	1.07880605E-5	35.616627	9
AGCCGTA	30	0.0039558485	31.659224	62-63
GACCGTG	60	0.0039545638	31.659224	7
ATCAACG	1615	0.0	31.169142	3
TCAACGC	1650	0.0	30.507977	4
ACCGTGC	95	3.4947207E-5	29.992947	8
TCGCACC	710	0.0	28.91138	1
CGCACCA	735	0.0	27.782583	2
CCTCGTC	125	7.3995634E-6	26.593746	8
CGCAGAG	1895	0.0	26.563673	8
TAGTCGT	45	8.914501E-4	26.406204	42-43
AAACCGT	90	8.95669E-4	26.382687	6
CCTATCC	90	8.95669E-4	26.382687	3
TATCAAC	1970	0.0	25.793425	2
CTCGTCC	130	1.0032822E-5	25.57091	9
GTCTAGT	130	1.0032822E-5	25.57091	9
AATGCGG	130	1.0032822E-5	25.57091	7
GCGAATT	75	1.0626154E-6	25.327377	14-15
>>END_MODULE