FastQCFastQC Report
Fri 10 Feb 2017
SRR3224536_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224536_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337646
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA34200.255673025598701No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA29250.21866771926204692No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA29000.21679876439655932No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG28620.2139579530010182No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA22760.17014965095398932No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA21590.16140294218350743No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC21190.1584126143987273No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG21120.15788930703639079No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC19580.1463765450649873No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA18030.1347890248989643No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC17530.13105111516798915No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC17330.12955595127559907No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC16740.12514521779304838No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC16630.12432287765223385No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA16090.12028593514278067No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA15780.11796843110957608No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG15760.11781891472033706No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA14540.10869841497675768No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT14340.10720325108436761No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT13970.10443719788344599No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG13900.10391389052110947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7750.049.6886831
GTATCAA16750.044.5614241
CCGTGCG1800.042.2371069
AATGCGG3900.032.8679247
AACCGTG2200.032.3699237
ATCAACG23900.030.1938723
GTACATG54000.029.8455871
AAATGCG4350.029.4677946
TCAACGC25250.028.3915214
ACATGGG54150.028.0559273
AAACCGT2450.027.129086
CGGACCG1405.833117E-727.129085
GACCGTA1606.27424E-826.7051897
TACATGG59650.026.2649522
CATGGGG41500.025.7399394
ATGCGGG4800.025.718998
ACCGTGC2409.094947E-1225.718998
CGCAGAG29150.024.7586448
GCCCATA15250.024.628131
ACGCAGA29500.024.4621527
Produced by FastQC (version 0.11.5)