##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224536_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1337646
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.324391505674896	34.0	31.0	34.0	31.0	34.0
2	32.49225505103742	34.0	31.0	34.0	31.0	34.0
3	32.63040669953037	34.0	31.0	34.0	31.0	34.0
4	35.90308048616749	37.0	37.0	37.0	35.0	37.0
5	35.91463959821956	37.0	37.0	37.0	35.0	37.0
6	36.00909358679352	37.0	37.0	37.0	35.0	37.0
7	35.96942090807284	37.0	37.0	37.0	35.0	37.0
8	36.010398117289625	37.0	37.0	37.0	35.0	37.0
9	37.75648116168254	39.0	39.0	39.0	35.0	39.0
10-11	37.828541706849194	39.0	39.0	39.0	37.0	39.0
12-13	37.81977257062033	39.0	39.0	39.0	37.0	39.0
14-15	39.33782966494872	41.0	40.0	41.0	37.0	41.0
16-17	39.174488616569704	41.0	40.0	41.0	36.5	41.0
18-19	39.259501392745165	41.0	40.0	41.0	36.5	41.0
20-21	39.31617034701259	41.0	40.0	41.0	37.0	41.0
22-23	39.276365346287434	41.0	40.0	41.0	37.0	41.0
24-25	39.22151338994024	41.0	40.0	41.0	36.0	41.0
26-27	39.060973157322636	41.0	39.5	41.0	36.0	41.0
28-29	39.005421090482834	41.0	39.0	41.0	35.5	41.0
30-31	38.93763858300328	41.0	39.0	41.0	35.0	41.0
32-33	38.756666562005194	41.0	39.0	41.0	35.0	41.0
34-35	38.76862749935334	41.0	39.0	41.0	35.0	41.0
36-37	38.75389415435773	41.0	39.0	41.0	35.0	41.0
38-39	38.63447242394475	41.0	39.0	41.0	35.0	41.0
40-41	38.54533075268046	40.5	39.0	41.0	35.0	41.0
42-43	38.410024027283754	40.0	38.0	41.0	34.5	41.0
44-45	38.23167564512584	40.0	38.0	41.0	34.0	41.0
46-47	38.065282593451485	40.0	38.0	41.0	34.0	41.0
48-49	37.85702644795409	40.0	37.0	41.0	33.0	41.0
50-51	36.57193569898165	38.5	35.5	40.0	31.5	40.5
52-53	36.86994914947602	39.0	35.5	40.0	32.5	40.5
54-55	37.2927138420778	39.0	35.5	41.0	33.0	41.0
56-57	37.132147444092084	39.0	35.0	41.0	33.0	41.0
58-59	36.976994286978766	39.0	35.0	41.0	33.0	41.0
60-61	36.72889763061378	39.0	35.0	41.0	32.0	41.0
62-63	36.4613821594054	38.0	35.0	41.0	32.0	41.0
64-65	36.23976373420172	37.0	35.0	41.0	32.0	41.0
66-67	36.03566601328005	37.0	35.0	40.0	31.0	41.0
68-69	35.76781599915075	36.0	35.0	40.0	31.0	41.0
70-71	35.51069378594934	36.0	35.0	39.5	31.0	41.0
72-73	35.2123992446432	35.5	35.0	39.0	30.5	41.0
74-75	34.94771449247409	35.0	35.0	39.0	30.0	41.0
76-77	34.646620630570425	35.0	34.5	37.5	30.0	40.0
78-79	34.40093716872775	35.0	34.0	37.0	30.0	39.0
80-81	34.08980402886863	35.0	34.0	37.0	30.0	39.0
82-83	33.81531473947517	35.0	34.0	36.0	29.0	38.5
84-85	33.568587279444635	35.0	34.0	36.0	29.0	37.0
86-87	33.33639206486619	35.0	34.0	35.5	29.0	37.0
88-89	33.184100651442904	35.0	34.0	35.0	29.0	36.5
90-91	33.00424364891758	35.0	34.0	35.0	29.0	36.0
92-93	32.869985780991385	35.0	34.0	35.0	29.0	36.0
94-95	32.731445016095435	35.0	34.0	35.0	27.0	36.0
96-97	32.598835566360606	35.0	33.0	35.0	27.0	35.5
98-99	32.5568846316589	35.0	33.0	35.0	27.0	35.0
100-101	31.353576357272402	34.5	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	12.0
7	482.0
8	2088.0
9	1492.0
10	1074.0
11	1406.0
12	1885.0
13	2096.0
14	2085.0
15	2231.0
16	2347.0
17	2492.0
18	2838.0
19	3139.0
20	3809.0
21	4911.0
22	5907.0
23	5994.0
24	6053.0
25	6232.0
26	6609.0
27	6936.0
28	7485.0
29	8681.0
30	10891.0
31	13793.0
32	18314.0
33	27179.0
34	46609.0
35	85900.0
36	194359.0
37	321094.0
38	386910.0
39	144301.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.12979248327474	20.414584199078288	13.32172767126775	25.133895646379223
2	15.85793251727288	21.123974504465306	37.75333683201684	25.26475614624497
3	18.451919942824844	28.435592728708087	28.789573309490418	24.32291401897665
4	12.725265129937219	17.920286832241118	39.70048876907642	29.653959268745243
5	13.478005391560997	39.28371183407269	34.39744147554734	12.840841298818969
6	27.85931404870945	38.55653887500878	20.22104502985095	13.36310204643082
7	25.256757019420682	31.529492855359344	24.91600916834499	18.29774095687499
8	25.07195476232127	34.39273170928631	22.073852125300714	18.4614614030917
9	26.825333458927098	15.526155649551526	21.029853937439352	36.61865695408203
10-11	25.538969204109307	25.38208913270028	28.004756116341696	21.074185546848717
12-13	25.94266345505463	23.365561591033803	28.206491104522424	22.485283849389152
14-15	23.398339210947583	24.952108443759066	25.300091127638147	26.3494612176552
16-17	23.13136281934503	27.25032684995202	26.765223539181783	22.853086791521164
18-19	22.881789708369276	27.076914103554913	27.006884442834224	23.03441174524159
20-21	24.098931076197047	25.656527128826166	26.37248357910915	23.87205821586764
22-23	24.247178016318845	26.558851890835673	25.73022965624502	23.46374043660047
24-25	23.412636103023317	26.42159523734752	26.190064946620545	23.975703713008613
26-27	23.178030659830778	27.27048112878893	26.734689147950952	22.81679906342934
28-29	23.032588592198532	27.11909578468444	26.737529959346496	23.110785663770532
30-31	23.303026361234586	26.462046012173623	26.702318849680708	23.532608776911083
32-33	23.216045201794795	26.48010011617423	27.01656492076379	23.287289761267182
34-35	23.679695524824954	26.1238773188123	26.749341754096374	23.447085402266367
36-37	22.928485089579176	26.231243968888908	27.380132075958446	23.460138865573473
38-39	23.038331365839472	26.668525755229812	26.470966444448262	23.822176434482454
40-41	23.701315450090277	26.01309095258922	27.25359141424903	23.032002183071477
42-43	23.47073993373951	26.607889730820023	26.989954765981892	22.931415569458572
44-45	23.325170011061793	26.115478548755444	26.612343376923803	23.947008063258956
46-47	23.503864251079882	26.037643741318707	26.49576195794702	23.962730049654393
48-49	22.93106696390525	26.547831040499503	26.67211653905443	23.84898545654082
50-51	22.83694639687929	26.92020160789925	26.43853455996579	23.804317435255665
52-53	23.362346988665163	26.52704826239528	26.04997884343092	24.06062590550863
54-55	23.20468195621263	26.092366739780182	26.437712219824977	24.265239084182213
56-57	23.21459609440618	26.535281582452154	26.367748405687458	23.882373917454213
58-59	23.702967076720046	26.385430238274598	26.67482910288595	23.236773582119405
60-61	23.410663407620202	26.61503934296666	26.57884987014036	23.39544737927278
62-63	22.8075075556772	26.234380602726738	27.307038118452233	23.651073723143835
64-65	23.998587869590356	25.835142750079093	26.159491453544426	24.006777926786125
66-67	23.043129109176515	26.56151511548875	25.915412183163937	24.479943592170798
68-69	23.027579793159028	26.58180863995407	27.238222967810614	23.152388599076286
70-71	23.699543825496434	25.95746557758929	26.752818010146186	23.590172586768098
72-73	23.298727765043964	26.321650122678196	27.08093172633118	23.298690385946657
74-75	23.265983675800623	26.01316043258082	27.007519179214828	23.71333671240373
76-77	23.12532613262403	25.748292149043916	26.374877957247282	24.751503761084773
78-79	23.364364739718642	25.241016488230784	27.04042930787415	24.35418946417643
80-81	22.810654694136463	26.440713085654355	26.52808343269716	24.220548787512026
82-83	22.805530789437466	26.787910111956155	26.67474224953476	23.73181684907162
84-85	23.145281875277394	25.52190851023177	27.29953639291327	24.033273221577563
86-87	22.809539874524155	26.893563958346267	26.803986442564543	23.492909724565035
88-89	23.186658111297575	25.960385051436912	26.50306018971381	24.3498966475517
90-91	22.916264841370587	25.784774148018236	26.76519049135571	24.53377051925547
92-93	22.85633330666872	25.784556404254477	27.243883274783325	24.115227014293477
94-95	23.14480064232241	25.307293558983467	27.145821839260908	24.402083959433213
96-97	23.49048253424299	26.158677258407682	26.94733883254613	23.4035013748032
98-99	22.23439125414583	26.23378720499806	27.277753086687405	24.254068454168706
100-101	23.107191432852822	25.664119573871485	26.791669050709622	24.437019942566067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	2.0
4	1.5
5	2.5
6	4.5
7	10.5
8	18.0
9	20.5
10	25.0
11	25.5
12	32.0
13	41.0
14	41.5
15	48.0
16	56.0
17	62.0
18	69.0
19	83.0
20	95.5
21	117.0
22	141.5
23	164.0
24	210.0
25	264.5
26	361.0
27	473.5
28	626.0
29	928.0
30	1656.5
31	3351.5
32	6898.5
33	12976.5
34	21510.0
35	32220.5
36	46884.5
37	59791.5
38	64563.5
39	63664.5
40	57277.0
41	50003.0
42	45558.5
43	41076.0
44	38078.5
45	38398.0
46	40502.0
47	39499.0
48	39970.0
49	46083.0
50	52123.5
51	52857.0
52	51332.0
53	51532.0
54	54385.0
55	55816.0
56	46779.0
57	38327.0
58	36786.0
59	35204.5
60	29323.5
61	22253.0
62	17906.5
63	12650.0
64	7531.5
65	4756.5
66	3710.5
67	3273.0
68	2419.0
69	1501.5
70	927.0
71	588.0
72	439.5
73	311.0
74	252.0
75	212.0
76	151.0
77	95.0
78	63.0
79	46.5
80	31.0
81	24.5
82	23.0
83	20.5
84	16.0
85	13.0
86	12.0
87	8.5
88	6.0
89	4.5
90	4.5
91	7.5
92	7.0
93	6.5
94	5.5
95	3.0
96	3.0
97	3.0
98	4.5
99	3.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15631938494938125
2	0.0
3	1.4951638923900643E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0381640583532564
16-17	0.07651501219306153
18-19	0.026576538187233392
20-21	0.041378660721895034
22-23	0.06795519890912843
24-25	0.01794196670868077
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.023137661234736245
38-39	0.005457348207223735
40-41	0.006055413764179761
42-43	0.0034762560498068995
44-45	0.011923932041810763
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	3.737909730975161E-5
58-59	0.016334665524361452
60-61	0.01797934580599052
62-63	0.03038920611282806
64-65	0.04926565025425262
66-67	0.01816624129253928
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.017755071222132015
80-81	0.0604046212525586
82-83	0.051882187065935226
84-85	0.030987271669784083
86-87	0.06092792861489512
88-89	0.03543538424964452
90-91	0.0
92-93	0.0032146023686386383
94-95	0.0
96-97	0.0
98-99	4.859282650267709E-4
100-101	0.047209799902216285
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1337646.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.346875513711694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.46645415534606	25.57008140204958
2	15.537852009926539	11.60580449530403
3	6.36790101497176	7.134636194693661
4	3.0524193551645373	4.559933026918964
5	1.7422006268586068	3.253287496559943
6	1.1070260717586988	2.480637893544324
7	0.7087867278420128	1.852967878334069
8	0.49398926201589055	1.475916437889422
9	0.38319302513925263	1.2879955986838438
>10	1.7736399354779466	11.829626140830094
>50	0.15899454348392628	4.180539377509922
>100	0.17644227437045623	14.034791666937322
>500	0.022563468879154776	5.793732685813394
>1k	0.008537528765085591	4.940049704931394
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	3420	0.255673025598701	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2925	0.21866771926204692	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	2900	0.21679876439655932	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2862	0.2139579530010182	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	2276	0.17014965095398932	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	2159	0.16140294218350743	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	2119	0.1584126143987273	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	2112	0.15788930703639079	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1958	0.1463765450649873	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	1803	0.1347890248989643	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1753	0.13105111516798915	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	1733	0.12955595127559907	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1674	0.12514521779304838	No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC	1663	0.12432287765223385	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1609	0.12028593514278067	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1578	0.11796843110957608	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1576	0.11781891472033706	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1454	0.10869841497675768	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1434	0.10720325108436761	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1397	0.10443719788344599	No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG	1390	0.10391389052110947	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	1.4951638923900643E-4	0.0
16-17	0.0	0.0	0.0	1.4951638923900643E-4	0.0
18-19	0.0	0.0	0.0	2.9903277847801287E-4	0.0
20-21	0.0	0.0	0.0	5.606864596462741E-4	0.0
22-23	0.0	0.0	0.0	6.354446542657774E-4	0.0
24-25	0.0	0.0	0.0	8.223401408145353E-4	0.0
26-27	0.0	0.0	0.0	0.0010839938219827966	0.0
28-29	0.0	0.0	0.0	0.0016820593789388224	0.0
30-31	0.0	0.0	0.0	0.005419969109913983	0.0
32-33	0.0	0.0	0.0	0.015288050799688407	0.0
34-35	0.0	0.0	0.0	0.027735290203835694	0.0
36-37	0.0	0.0	0.0	0.05061129775740368	0.0
38-39	0.0	0.0	0.0	0.09823226773002722	0.0
40-41	0.0	0.0	0.0	0.16458016545483634	0.0
42-43	0.0	0.0	0.0	0.22920862470339687	0.0
44-45	0.0	0.0	0.0	0.29892064118608364	0.0
46-47	0.0	0.0	0.0	0.37879977213702276	0.0
48-49	0.0	0.0	0.0	0.4674256118584439	0.0
50-51	0.0	0.0	0.0	0.5661438078534978	0.0
52-53	0.0	0.0	0.0	0.6632920817615422	0.0
54-55	0.0	0.0	0.0	0.761748624075428	0.0
56-57	0.0	0.0	0.0	0.8746334979508779	0.0
58-59	0.0	0.0	0.0	1.0048622729780523	0.0
60-61	0.0	0.0	0.0	1.1503417197076056	0.0
62-63	0.0	0.0	0.0	1.301091619157834	0.0
64-65	0.0	0.0	0.0	1.446608444984697	0.0
66-67	0.0	0.0	0.0	1.6129080489157819	0.0
68-69	0.0	0.0	0.0	1.808512865137712	0.0
70-71	0.0	0.0	0.0	2.0345816456670898	0.0
72-73	0.0	0.0	0.0	2.2778074318616435	0.0
74-75	0.0	0.0	0.0	2.5019324993309144	0.0
76-77	0.0	0.0	0.0	2.7434388470492195	0.0
78-79	0.0	0.0	0.0	3.0216140892283905	0.0
80-81	0.0	0.0	0.0	3.3366825004522873	0.0
82-83	0.0	0.0	0.0	3.68389693536257	0.0
84-85	0.0	0.0	0.0	4.031896331316357	0.0
86-87	0.0	0.0	0.0	4.393202685912416	0.0
88-89	0.0	0.0	0.0	4.789570633785022	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	775	0.0	49.688683	1
GTATCAA	1675	0.0	44.561424	1
CCGTGCG	180	0.0	42.237106	9
AATGCGG	390	0.0	32.867924	7
AACCGTG	220	0.0	32.369923	7
ATCAACG	2390	0.0	30.193872	3
GTACATG	5400	0.0	29.845587	1
AAATGCG	435	0.0	29.467794	6
TCAACGC	2525	0.0	28.391521	4
ACATGGG	5415	0.0	28.055927	3
AAACCGT	245	0.0	27.12908	6
CGGACCG	140	5.833117E-7	27.12908	5
GACCGTA	160	6.27424E-8	26.705189	7
TACATGG	5965	0.0	26.264952	2
CATGGGG	4150	0.0	25.739939	4
ATGCGGG	480	0.0	25.71899	8
ACCGTGC	240	9.094947E-12	25.71899	8
CGCAGAG	2915	0.0	24.758644	8
GCCCATA	1525	0.0	24.62813	1
ACGCAGA	2950	0.0	24.462152	7
>>END_MODULE