FastQCFastQC Report
Fri 10 Feb 2017
SRR3224536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337646
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT29670.22180756343606603No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT29170.21806965370509088No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG26950.20147333449956117No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA24240.18121386375767579No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG23640.1767283720805056No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA23450.17530796638273505No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG21080.15759027425791278No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC20640.15430091369465465No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC19810.14809598354123588No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA18830.14076968046852456No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG18640.139349274770754No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT17880.13366765197967176No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA17830.13329386100657423No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG17800.13306958642271574No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG17760.1327705536442377No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA17580.13142490614108665No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC17530.13105111516798915No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT16830.1258180415446239No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT16610.12417336126299483No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA16190.12103351708897571No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG16090.12028593514278067No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA16080.12021117694816118No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC14930.1116139845669183No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC14610.10922172233909419No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT250.00493973657.2367671
GGTATCA8350.054.8376161
GTATCAA18750.046.0437971
ACCGTGC2300.041.292978
CCGTGCG1900.039.9889839
AACCGTG2450.036.8265887
AAACCGT2300.035.0990266
ATCAACG26700.032.1915053
TCAACGC27100.031.3658984
GTACATG58450.028.3980541
CGCAGAG31400.027.3730328
ACGCAGA31550.026.9418627
ACATGGG60400.026.2593213
AATGCGG4100.025.4807837
AAATGCG4100.025.4807836
CATGGGG48550.024.6482054
TACATGG66000.024.5349062
GTGCGCG1650.024.46295710-11
ATGCGGG4150.024.0295228
TAATACT10850.023.634044
Produced by FastQC (version 0.11.5)