##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224536_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1337646
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.19958942799515	34.0	33.0	34.0	31.0	34.0
2	33.326907866505785	34.0	34.0	34.0	31.0	34.0
3	33.44330189003668	34.0	34.0	34.0	31.0	34.0
4	36.65158644364802	37.0	37.0	37.0	35.0	37.0
5	36.682662677569404	37.0	37.0	37.0	35.0	37.0
6	36.72072506477797	37.0	37.0	37.0	37.0	37.0
7	36.6985286092135	37.0	37.0	37.0	36.0	37.0
8	36.69079337881622	37.0	37.0	37.0	36.0	37.0
9	38.54594264850341	39.0	39.0	39.0	38.0	39.0
10-11	38.57054557035269	39.0	39.0	39.0	37.5	39.0
12-13	38.553548547224004	39.0	39.0	39.0	37.5	39.0
14-15	40.17209112126825	41.0	40.0	41.0	38.0	41.0
16-17	40.18120115486459	41.0	40.0	41.0	38.0	41.0
18-19	40.17395147894136	41.0	40.0	41.0	38.5	41.0
20-21	40.17615348156389	41.0	40.0	41.0	39.0	41.0
22-23	40.11034608558617	41.0	40.0	41.0	38.0	41.0
24-25	40.07126362281201	41.0	40.0	41.0	38.0	41.0
26-27	40.01448776432629	41.0	40.0	41.0	38.0	41.0
28-29	39.99295852564879	41.0	40.0	41.0	38.0	41.0
30-31	39.93849456433167	41.0	40.0	41.0	38.0	41.0
32-33	39.90501859236301	41.0	40.0	41.0	38.0	41.0
34-35	39.82874056364688	41.0	40.0	41.0	38.0	41.0
36-37	39.711055092303944	41.0	40.0	41.0	37.0	41.0
38-39	39.602497596524046	41.0	40.0	41.0	37.0	41.0
40-41	39.4808869461726	41.0	39.5	41.0	36.0	41.0
42-43	39.39082313257768	41.0	39.0	41.0	36.0	41.0
44-45	39.154011973272446	41.0	39.0	41.0	35.0	41.0
46-47	38.94737583785246	41.0	38.5	41.0	35.0	41.0
48-49	38.8491858085024	40.0	38.0	41.0	35.0	41.0
50-51	38.67579127811095	40.0	37.5	41.0	35.0	41.0
52-53	38.41890754355039	40.0	37.0	41.0	35.0	41.0
54-55	38.21660289792666	40.0	36.5	41.0	35.0	41.0
56-57	37.97322086710535	40.0	35.5	41.0	35.0	41.0
58-59	37.705960321340626	39.0	35.0	41.0	34.0	41.0
60-61	37.46172006644508	39.0	35.0	41.0	34.0	41.0
62-63	37.25631931019119	38.5	35.0	41.0	34.0	41.0
64-65	37.01545849948342	38.0	35.0	41.0	33.5	41.0
66-67	36.7630202609659	37.0	35.0	40.5	33.0	41.0
68-69	36.51139950330655	37.0	35.0	40.0	33.0	41.0
70-71	36.23004255236438	36.0	35.0	40.0	33.0	41.0
72-73	35.943285069442894	36.0	35.0	39.0	33.0	41.0
74-75	35.65123059464163	35.0	35.0	39.0	33.0	41.0
76-77	33.812325906854284	34.5	32.5	36.5	29.5	39.0
78-79	35.08755305962863	35.0	35.0	37.0	32.5	39.0
80-81	34.99233616367858	35.0	35.0	37.0	33.0	39.0
82-83	34.770056128452524	35.0	35.0	36.5	33.0	39.0
84-85	34.54343413728296	35.0	35.0	36.0	33.0	37.0
86-87	34.335692328164555	35.0	35.0	36.0	33.0	37.0
88-89	34.18354706701175	35.0	35.0	35.5	32.5	36.5
90-91	34.03099175716146	35.0	35.0	35.0	32.0	36.0
92-93	33.92552140102838	35.0	35.0	35.0	32.0	36.0
94-95	33.8167272955625	35.0	35.0	35.0	32.0	36.0
96-97	33.76675742311494	35.0	34.5	35.0	32.0	36.0
98-99	33.740096781958755	35.0	34.0	35.0	32.0	35.0
100-101	32.92641812557284	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	3.0
14	18.0
15	54.0
16	131.0
17	282.0
18	459.0
19	718.0
20	1022.0
21	1361.0
22	1937.0
23	2144.0
24	2390.0
25	3439.0
26	4602.0
27	6944.0
28	9395.0
29	10092.0
30	9633.0
31	9928.0
32	11767.0
33	15815.0
34	27541.0
35	59751.0
36	178617.0
37	358266.0
38	433823.0
39	187494.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.07604293114431	20.93974976863815	13.62728807855486	24.356919221662682
2	15.246186210701488	21.396841914826496	38.87672822256412	24.480243651907905
3	17.893523398567336	29.527393645254424	29.244583395008846	23.334499561169398
4	12.55332128231236	18.3951508844642	40.40127208543964	28.650255747783792
5	13.200727247717259	38.907528598747355	35.29999715918861	12.591746994346787
6	27.38407620551327	38.583451825071805	20.73665229814166	13.295819671273268
7	24.963630138317612	31.533305523284934	25.367399147457547	18.135665190939903
8	25.05296618088792	34.441025503010515	22.144872410189244	18.361135905912327
9	26.50342467289552	15.692941181747639	21.498662575898255	36.30497156945859
10-11	25.532091450204312	25.421673596751308	28.380939351667035	20.665295601377345
12-13	25.987555750923637	23.706795370374522	28.380789835277792	21.924859043424043
14-15	23.500088962251596	25.365343297105515	25.543678970370337	25.590888770272553
16-17	22.900490862305872	27.572915405122135	26.902857706747525	22.62373602582447
18-19	22.725108137728515	27.12092736045262	27.578671786107833	22.575292715711033
20-21	23.893972689889967	25.946286603273762	26.755736704254108	23.404004002582166
22-23	24.177050719632064	26.601267317968823	26.083186057708126	23.138495904690988
24-25	23.868766229567147	26.387561409299266	26.310485566595286	23.433186794538305
26-27	23.314208476514327	27.41565482106752	26.858019898872133	22.412116803546017
28-29	23.157428859577706	27.044798860048697	27.185685417400258	22.61208686297334
30-31	23.33864118895966	26.31335785413116	27.18810741305583	23.159893543853354
32-33	23.386885160113483	26.460458492350362	27.451379102826234	22.70127724470992
34-35	24.051395189578916	26.25830220784153	26.692289749670305	22.99801285290925
36-37	22.88827818426351	26.36808593192222	27.310396025170135	23.433239858644143
38-39	23.04380232139146	26.521179744117653	26.72026081638939	23.714757118101502
40-41	23.283514472438895	26.303670776872206	27.48025262289126	22.932562127797638
42-43	23.46984927252801	26.54491547090934	27.355593333363238	22.62964192319941
44-45	23.37464940156684	26.04411325464876	27.097170203833993	23.484067139950408
46-47	23.47162691034946	25.92868028678716	26.653354291968956	23.946338510894424
48-49	22.917600374093503	26.036653537759037	27.131422770138457	23.914323318009007
50-51	23.07516338403434	26.36059914207496	26.869552931044538	23.694684542846165
52-53	23.399257650572462	26.327725218574432	26.56754968620224	23.70546744465086
54-55	23.38492396344025	25.909657712130112	26.808139074164615	23.89727925026502
56-57	23.607771129173287	26.309745387149025	26.38589261409281	23.69659086958488
58-59	23.78032044143526	26.18819334182439	26.890612694853687	23.14087352188666
60-61	23.373310176053902	26.76524089864702	26.615192197317967	23.24625672798111
62-63	22.802333352770464	26.39472625791876	27.309280631796458	23.493659757514322
64-65	23.60661938958439	26.350432027606708	26.28094428570788	23.76200429710103
66-67	23.095385475678917	26.71528939644719	26.168433202805524	24.020891925068366
68-69	23.29633550281614	26.661500875418458	26.989203421533052	23.05296020023235
70-71	23.888943711564945	26.2139609433288	26.708299505250267	23.188795839855985
72-73	23.301830230120675	26.50925581207584	26.894970717215166	23.293943240588316
74-75	23.375728705502052	26.043512259596334	26.844322040360453	23.736436994541158
76-77	23.196976470535464	25.670171839700007	26.713089152404006	24.41976253736052
78-79	23.509500290832857	25.518377795621504	26.639803878469248	24.332318035076387
80-81	22.613405703648045	26.606146243360136	26.682441600968925	24.098006452022894
82-83	22.818521258299658	26.742886021214378	26.827662796265482	23.61092992422048
84-85	23.206281672735347	25.729409754968863	27.139205094476658	23.925103477819132
86-87	22.669525420028915	26.904053837861436	27.01095805616733	23.41546268594232
88-89	23.32362224385226	25.935486668371155	26.271861165061612	24.469029922714977
90-91	23.262993348015844	25.995442740455992	26.42941406022221	24.31214985130595
92-93	23.378391360042702	25.976426489679067	26.900213845895642	23.74496830438259
94-95	23.24243484449548	25.53429681694559	26.56951091693916	24.653757421619773
96-97	23.634803228955942	26.266553333243625	26.66964951863198	23.42899391916845
98-99	22.011204758209573	26.478492814989913	27.2178513597768	24.292451067023713
100-101	22.947467327687427	25.995935575163244	26.755855094614017	24.30074200253531
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	1.5
3	3.5
4	3.5
5	2.5
6	7.5
7	14.0
8	15.0
9	18.0
10	22.5
11	25.5
12	27.0
13	25.0
14	29.5
15	35.0
16	37.0
17	42.5
18	53.5
19	62.5
20	77.0
21	96.5
22	108.5
23	143.5
24	197.0
25	256.0
26	372.0
27	507.0
28	638.5
29	974.5
30	1893.0
31	4050.0
32	8323.0
33	15191.5
34	24174.0
35	35429.0
36	50575.5
37	62194.0
38	64567.0
39	62244.0
40	55850.0
41	48860.0
42	44355.5
43	40667.0
44	38857.5
45	38750.5
46	38863.5
47	38998.5
48	40490.5
49	46203.0
50	52945.5
51	52935.0
52	49630.5
53	49794.0
54	53896.0
55	55150.5
56	46046.0
57	37883.0
58	36519.5
59	36261.0
60	30181.5
61	21281.0
62	16721.0
63	11749.5
64	6827.5
65	4141.5
66	3162.0
67	2702.0
68	2009.0
69	1258.0
70	717.5
71	407.5
72	295.0
73	241.5
74	187.5
75	133.0
76	84.5
77	53.0
78	41.0
79	25.0
80	11.5
81	7.5
82	5.0
83	4.0
84	1.0
85	0.0
86	0.0
87	1.0
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4783029291755816
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.102028488852806E-4
22-23	0.0013456475031510579
24-25	1.8689548654875803E-4
26-27	0.002429641325133855
28-29	0.003364118757877645
30-31	4.485491677170193E-4
32-33	7.475819461950322E-5
34-35	0.003737909730975161
36-37	0.002167987643965593
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.008335538700074608
46-47	0.07812231337738086
48-49	9.344774327437902E-4
50-51	0.0
52-53	7.475819461950322E-5
54-55	0.0
56-57	0.007886989532357589
58-59	0.04134128162458528
60-61	0.03091251347516458
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.9903277847801287E-4
78-79	0.008073885018906348
80-81	0.006055413764179761
82-83	0.0011587520166022997
84-85	0.0030277068820898805
86-87	0.0
88-89	0.0
90-91	0.0
92-93	3.737909730975161E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06918870912035023
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1337646.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.39764158528577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.70315218742537	23.611342737392537
2	16.313989131809905	11.549534802281086
3	6.648135460112192	7.059849486824407
4	3.30849465113135	4.684516313903304
5	1.8634542263629288	3.2980942407690463
6	1.1522539687077535	2.4472243799724085
7	0.7750434637722341	1.9204297520539568
8	0.5665288114693167	1.604302705290304
9	0.37702075298748333	1.201108093601894
>10	1.894607968772833	12.040564811359783
>50	0.16857152937141312	4.191778549665665
>100	0.1922838737919954	14.502950425145736
>500	0.026923981012974833	6.50382215138487
>1k	0.009539993272313916	5.384481550355001
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	2967	0.22180756343606603	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	2917	0.21806965370509088	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2695	0.20147333449956117	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2424	0.18121386375767579	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	2364	0.1767283720805056	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2345	0.17530796638273505	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	2108	0.15759027425791278	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	2064	0.15430091369465465	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	1981	0.14809598354123588	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1883	0.14076968046852456	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1864	0.139349274770754	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1788	0.13366765197967176	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1783	0.13329386100657423	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	1780	0.13306958642271574	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	1776	0.1327705536442377	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	1758	0.13142490614108665	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1753	0.13105111516798915	No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT	1683	0.1258180415446239	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	1661	0.12417336126299483	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1619	0.12103351708897571	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1609	0.12028593514278067	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1608	0.12021117694816118	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1493	0.1116139845669183	No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC	1461	0.10922172233909419	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	1.4951638923900643E-4	0.0
16-17	0.0	0.0	0.0	1.4951638923900643E-4	0.0
18-19	0.0	0.0	0.0	2.9903277847801287E-4	0.0
20-21	0.0	0.0	0.0	4.8592826502677093E-4	0.0
22-23	0.0	0.0	0.0	5.606864596462741E-4	0.0
24-25	0.0	0.0	0.0	7.475819461950321E-4	0.0
26-27	0.0	0.0	0.0	0.0010092356273632935	0.0
28-29	0.0	0.0	0.0	0.0016073011843193191	0.0
30-31	0.0	0.0	0.0	0.0053825900126042315	0.0
32-33	0.0	0.0	0.0	0.015512325383546917	0.0
34-35	0.0	0.0	0.0	0.028221218468862466	0.0
36-37	0.0	0.0	0.0	0.051134605119740204	0.0
38-39	0.0	0.0	0.0	0.09927888245470026	0.0
40-41	0.0	0.0	0.0	0.16573891747143865	0.0
42-43	0.0	0.0	0.0	0.2303299976226894	0.0
44-45	0.0	0.0	0.0	0.3004531841757834	0.0
46-47	0.0	0.0	0.0	0.3806687270025104	0.0
48-49	0.0	0.0	0.0	0.4698926322808875	0.0
50-51	0.0	0.0	0.0	0.5693584102221365	0.0
52-53	0.0	0.0	0.0	0.666955233297898	0.0
54-55	0.0	0.0	0.0	0.7666079067256957	0.0
56-57	0.0	0.0	0.0	0.8811374608827747	0.0
58-59	0.0	0.0	0.0	1.0131604325808174	0.0
60-61	0.0	0.0	0.0	1.1598733895215925	0.0
62-63	0.0	0.0	0.0	1.3124174856426887	0.0
64-65	0.0	0.0	0.0	1.4595042335565613	0.0
66-67	0.0	0.0	0.0	1.6272616222827265	0.0
68-69	0.0	0.0	0.0	1.8242868442024274	0.0
70-71	0.0	0.0	0.0	2.0527478869596294	0.0
72-73	0.0	0.0	0.0	2.298777105452414	7.475819461950322E-5
74-75	0.0	0.0	0.0	2.5245842323006236	7.475819461950322E-5
76-77	0.0	0.0	0.0	2.767548364814009	7.475819461950322E-5
78-79	0.0	0.0	0.0	3.0488634511671995	7.475819461950322E-5
80-81	0.0	0.0	0.0	3.3680809421924787	7.475819461950322E-5
82-83	0.0	0.0	0.0	3.7191828032229752	7.475819461950322E-5
84-85	0.0	0.0	0.0	4.070172526961543	7.475819461950322E-5
86-87	0.0	0.0	0.0	4.435777477748223	7.475819461950322E-5
88-89	0.0	0.0	0.0	4.835360027989468	7.475819461950322E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAT	25	0.004939736	57.236767	1
GGTATCA	835	0.0	54.837616	1
GTATCAA	1875	0.0	46.043797	1
ACCGTGC	230	0.0	41.29297	8
CCGTGCG	190	0.0	39.988983	9
AACCGTG	245	0.0	36.826588	7
AAACCGT	230	0.0	35.099026	6
ATCAACG	2670	0.0	32.191505	3
TCAACGC	2710	0.0	31.365898	4
GTACATG	5845	0.0	28.398054	1
CGCAGAG	3140	0.0	27.373032	8
ACGCAGA	3155	0.0	26.941862	7
ACATGGG	6040	0.0	26.259321	3
AATGCGG	410	0.0	25.480783	7
AAATGCG	410	0.0	25.480783	6
CATGGGG	4855	0.0	24.648205	4
TACATGG	6600	0.0	24.534906	2
GTGCGCG	165	0.0	24.462957	10-11
ATGCGGG	415	0.0	24.029522	8
TAATACT	1085	0.0	23.63404	4
>>END_MODULE