##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224534_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	920483
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.189929634767836	34.0	31.0	34.0	31.0	34.0
2	32.35418796436219	34.0	31.0	34.0	31.0	34.0
3	32.49726719559188	34.0	31.0	34.0	31.0	34.0
4	35.78153969166188	37.0	37.0	37.0	35.0	37.0
5	35.789446410199865	37.0	37.0	37.0	35.0	37.0
6	35.88528739802908	37.0	37.0	37.0	35.0	37.0
7	35.84799936555048	37.0	37.0	37.0	35.0	37.0
8	35.88757424091482	37.0	37.0	37.0	35.0	37.0
9	37.61403198103604	39.0	39.0	39.0	35.0	39.0
10-11	37.68199358380329	39.0	39.0	39.0	35.0	39.0
12-13	37.666897161598854	39.0	39.0	39.0	35.0	39.0
14-15	39.14589731695208	41.0	40.0	41.0	36.0	41.0
16-17	38.97495445325987	41.0	39.5	41.0	35.5	41.0
18-19	39.05725852623025	41.0	39.5	41.0	36.0	41.0
20-21	39.11289562110327	41.0	40.0	41.0	36.0	41.0
22-23	39.05830688888334	41.0	40.0	41.0	36.0	41.0
24-25	38.97920167998757	41.0	39.0	41.0	35.0	41.0
26-27	38.796767566592756	41.0	39.0	41.0	35.0	41.0
28-29	38.717529818584374	41.0	39.0	41.0	35.0	41.0
30-31	38.63931924869878	41.0	39.0	41.0	35.0	41.0
32-33	38.43359138626134	40.0	38.5	41.0	34.0	41.0
34-35	38.417938734338385	40.0	38.0	41.0	34.0	41.0
36-37	38.39405344802674	40.0	38.0	41.0	34.5	41.0
38-39	38.252056800614454	40.0	38.0	41.0	34.0	41.0
40-41	38.12913763752291	40.0	38.0	41.0	34.0	41.0
42-43	37.93834215297838	40.0	37.5	41.0	33.0	41.0
44-45	37.71340372391451	40.0	37.0	41.0	33.0	41.0
46-47	37.483724848802204	40.0	36.5	41.0	32.5	41.0
48-49	37.225103016568475	40.0	36.0	41.0	32.0	41.0
50-51	35.87821285129655	38.0	34.0	40.0	29.5	40.5
52-53	36.16730129725373	38.5	35.0	40.0	30.5	40.5
54-55	36.5280428861804	39.0	35.0	41.0	31.0	41.0
56-57	36.30358898534791	38.0	35.0	41.0	31.0	41.0
58-59	36.09419239681776	37.0	35.0	41.0	31.0	41.0
60-61	35.78701779391906	37.0	35.0	40.0	30.5	41.0
62-63	35.474008754099756	36.0	35.0	40.0	30.0	41.0
64-65	35.19713672061299	35.5	35.0	39.5	30.0	41.0
66-67	34.9439766948439	35.0	35.0	39.0	29.5	41.0
68-69	34.64330465635976	35.0	34.0	39.0	29.0	41.0
70-71	34.37375921119673	35.0	34.0	37.5	29.0	40.5
72-73	34.080282308309876	35.0	34.0	37.0	29.0	40.0
74-75	33.84123009333143	35.0	34.0	36.5	29.0	39.0
76-77	33.567968664277345	35.0	34.0	36.0	28.0	39.0
78-79	33.39492961847204	35.0	34.0	36.0	28.0	38.0
80-81	33.15641570784034	35.0	34.0	35.0	27.0	37.0
82-83	32.94743846437142	35.0	34.0	35.0	27.0	37.0
84-85	32.77316745665047	35.0	33.0	35.0	27.0	36.0
86-87	32.603226241006084	35.0	33.0	35.0	26.5	36.0
88-89	32.48826974534022	35.0	33.0	35.0	26.0	36.0
90-91	32.34715307072483	35.0	33.0	35.0	25.5	35.5
92-93	32.24193602706406	35.0	33.0	35.0	25.0	35.0
94-95	32.12186156615603	35.0	33.0	35.0	25.0	35.0
96-97	31.99791087939701	35.0	33.0	35.0	25.0	35.0
98-99	31.96343278474453	35.0	33.0	35.0	25.0	35.0
100-101	30.672792436144938	34.0	30.0	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	9.0
7	268.0
8	804.0
9	497.0
10	503.0
11	950.0
12	1489.0
13	1829.0
14	1870.0
15	1963.0
16	2137.0
17	2374.0
18	2532.0
19	3072.0
20	3574.0
21	4755.0
22	5725.0
23	5421.0
24	5172.0
25	5582.0
26	5676.0
27	6019.0
28	6603.0
29	7761.0
30	9560.0
31	12168.0
32	16424.0
33	23507.0
34	40306.0
35	74685.0
36	164224.0
37	259052.0
38	199499.0
39	44463.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.36342668146311	18.188306460386883	12.931327110124682	24.51693974802533
2	16.854539252261315	19.19385713137248	37.25635047724996	26.695253139116243
3	20.494633234834	25.720493655484095	27.44546323657222	26.33940987310968
4	13.481291886976729	16.11002050010701	37.50020369740669	32.90848391550957
5	14.254690200688117	38.85862096312479	32.91326401465318	13.97342482153391
6	30.464223673875562	36.21316200299191	18.862597136503336	14.460017186629193
7	27.857114145508387	30.522453972533985	21.58508087601835	20.035351005939273
8	27.23754811332746	32.93357943601348	19.99841387619326	19.830458574465798
9	27.77259330156016	14.671319296499771	19.22316870599457	38.3329186959455
10-11	27.409034170104174	23.791965739725775	26.355945737183628	22.443054352986422
12-13	27.595458036704645	21.76303093050062	26.653235312330594	23.98827572046415
14-15	24.79344276904334	23.505329869384646	23.851416815511044	27.849810546060972
16-17	24.614685467120314	26.105987113509943	25.04461565306825	24.234711766301498
18-19	24.61021461559359	25.71692474870959	25.493507199130672	24.17935343656615
20-21	25.996418896705002	23.58050553875498	25.23161695780113	25.191458606738887
22-23	26.084924095128812	24.859314130226224	24.29499433886474	24.760767435780224
24-25	25.06012837891065	24.663310958930158	25.009711269690786	25.266849392468398
26-27	24.844728256795616	25.670164468002127	25.409105871591326	24.07600140361093
28-29	24.56465790242731	25.821552380652328	25.363151736642607	24.250637980277745
30-31	24.632665676606738	24.551621268399305	25.70927437008614	25.10643868490782
32-33	24.618596975718184	25.020614177556784	25.78662506531897	24.574163781406067
34-35	25.427574436464333	24.17801306488007	25.265376981432574	25.129035517223024
36-37	24.376680883431582	24.38765586373638	26.39520795414414	24.840455298687893
38-39	24.562952162939993	24.97579953869738	24.90241048545419	25.558837812908436
40-41	25.424202982947136	24.425813096459052	25.545015818659067	24.604968101934745
42-43	25.161863674363804	24.879664903218586	25.5880136954676	24.370457726950008
44-45	25.149163851254997	24.27767646098543	25.223969868983538	25.34918981877604
46-47	25.0665683125055	24.2625339088283	25.090849043382658	25.58004873528354
48-49	24.535651391715003	24.781609220376694	25.4738001679553	25.208939219953002
50-51	24.572479882844114	25.217467351379657	24.945816489821105	25.26423627595512
52-53	25.134684725301824	25.07862719898141	24.099358706244438	25.687329369472334
54-55	25.085797347696808	24.15471008155501	24.8039887754581	25.95550379529008
56-57	24.98965217174027	24.898395733544238	24.72164070384787	25.39031139086762
58-59	25.44858421887563	25.092683138839995	25.070028467740187	24.38870417454419
60-61	25.10757034448588	25.295112177397623	24.589059552681217	25.008257925435277
62-63	24.685424724165923	24.70944100742331	25.418627724788607	25.186506543622155
64-65	25.787763806178205	24.055830120614193	24.537004329154897	25.619401744052713
66-67	24.649037406310374	24.776763885840452	24.175237904829373	26.3989608030198
68-69	24.786986831913246	24.952008890984406	25.626817659859007	24.63418661724334
70-71	25.4692373460455	24.15031021757056	25.17053546887884	25.2099169675051
72-73	25.019690749313135	24.599530898452226	25.466467061314553	24.914311290920093
74-75	24.949510202795704	24.44499246591192	25.540124043572774	25.0653732877196
76-77	24.971672480643313	23.872412635540254	24.76732324225434	26.388591641562094
78-79	25.05628440357004	23.336133065891868	25.505310074908238	26.102272455629855
80-81	24.8332645792604	24.938759959149753	24.651514682119757	25.57646077947009
82-83	24.480366251713274	25.268444375847128	25.09551391649991	25.15567545593969
84-85	24.692204580784786	24.196395727730575	25.768422219408865	25.342977472075773
86-87	24.81603289803013	25.530921528567013	24.753093566002324	24.899952007400536
88-89	25.051946922851215	24.542095482356522	24.448634491452665	25.957323103339604
90-91	24.56232217216396	24.34325240118503	24.975094597075664	26.119330829575343
92-93	24.55676812011451	24.559647131038453	25.469468903965975	25.414115844881064
94-95	24.98389432504457	24.083823384027735	25.51785312710827	25.41442916381943
96-97	25.25521927075242	24.98144995616432	25.149242299966428	24.61408847311683
98-99	23.87498669169714	25.368884427438786	25.287405022173264	25.46872385869081
100-101	24.866869154891535	24.487706421420057	24.717801736529452	25.92762268715895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	2.0
2	2.5
3	4.0
4	4.5
5	4.0
6	6.5
7	15.0
8	30.0
9	32.5
10	26.5
11	30.0
12	46.0
13	53.5
14	59.5
15	65.0
16	68.0
17	79.0
18	87.5
19	103.0
20	123.0
21	147.5
22	175.0
23	247.0
24	336.5
25	457.5
26	660.5
27	901.0
28	1171.0
29	1576.0
30	2402.0
31	3650.0
32	5197.5
33	6933.0
34	8642.0
35	10537.5
36	12958.0
37	14909.0
38	15880.0
39	16775.0
40	17488.0
41	17960.0
42	18755.0
43	19944.0
44	21656.0
45	25303.0
46	30092.5
47	31766.0
48	34278.5
49	40407.5
50	47018.0
51	48477.0
52	46968.5
53	48239.0
54	51737.5
55	52414.0
56	44435.0
57	37645.0
58	36021.0
59	34323.5
60	29028.5
61	22340.5
62	18342.0
63	13315.0
64	8016.0
65	5159.0
66	3925.5
67	3389.5
68	2586.0
69	1673.5
70	1042.5
71	652.0
72	438.5
73	302.0
74	216.5
75	176.0
76	140.5
77	94.0
78	61.5
79	40.5
80	34.0
81	28.5
82	21.5
83	16.5
84	14.0
85	14.0
86	12.0
87	8.0
88	9.0
89	9.0
90	7.5
91	6.0
92	5.0
93	6.0
94	3.5
95	3.0
96	4.5
97	5.5
98	4.5
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1459016624967544
2	1.0863861690004053E-4
3	3.2591585070012154E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03677417182066372
16-17	0.07414585603427766
18-19	0.025312797737709444
20-21	0.04079380064596522
22-23	0.0667584300850749
24-25	0.017436498012456503
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.022596832315208426
38-39	0.004997376377401864
40-41	0.0055405694619020665
42-43	0.0035307550492513174
44-45	0.011135458232254154
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.015535322216705795
60-61	0.01683898561950628
62-63	0.029766981030611102
64-65	0.04801826866981791
66-67	0.017056262853306362
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.017056262853306362
80-81	0.058393256583771785
82-83	0.04997376377401864
84-85	0.029278107254560922
86-87	0.05969691998657226
88-89	0.034003887089712685
90-91	0.0
92-93	0.0030418812732011346
94-95	0.0
96-97	0.0
98-99	5.431930845002026E-4
100-101	0.04666028595856741
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	920483.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.782180459507295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.16612990037252	32.03372702821862
2	14.716521446452088	12.886427954095495
3	5.252900511574893	6.899503144008305
4	2.4059970832351683	4.213591939330013
5	1.2853327475027205	2.8137335150839213
6	0.7447236868750534	1.9563376110739914
7	0.47187135778084377	1.4461689858023543
8	0.3268232296906225	1.1447226896539069
9	0.22936841103494843	0.90380242472783
>10	1.1190577179891625	8.982478174135625
>50	0.11929074671717366	3.7342474661404066
>100	0.13737176580691027	13.052754295786789
>500	0.01808184120091258	5.392320710093871
>1k	0.006529553766996211	4.540184061848947
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	3403	0.36969721331083794	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2695	0.2927810725456092	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2396	0.2602981260924971	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1999	0.217168595183181	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1889	0.20521834732417657	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	1771	0.19239899052997175	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1716	0.18642386660046956	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1686	0.18316470809346833	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1686	0.18316470809346833	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1630	0.17708094554706605	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1604	0.174256341507665	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1536	0.16686891555846223	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1515	0.1645875046035614	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	1492	0.16208881641486048	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1457	0.15828646482335904	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1450	0.15752599450505875	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1390	0.1510076774910563	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC	1248	0.13558099389125058	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	1226	0.13319094431944967	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1201	0.13047497889694867	No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC	1075	0.11678651316754357	No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT	1074	0.11667787455064353	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	1073	0.11656923593374349	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	1038	0.11276688434224205	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	1021	0.11092002785494136	No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC	1021	0.11092002785494136	No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG	971	0.10548809700993934	No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG	965	0.10483626530853911	No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC	928	0.10081663648323762	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	5.431930845002026E-5	0.0
16-17	0.0	0.0	0.0	1.0863861690004052E-4	0.0
18-19	0.0	0.0	0.0	2.715965422501013E-4	0.0
20-21	0.0	0.0	0.0	7.061510098502633E-4	0.0
22-23	0.0	0.0	0.0	8.691089352003241E-4	0.0
24-25	0.0	0.0	0.0	0.0011407054774504254	0.0
26-27	0.0	0.0	0.0	0.0015209406366005673	0.0
28-29	0.0	0.0	0.0	0.001846856487300689	0.0
30-31	0.0	0.0	0.0	0.003910990208401459	0.0
32-33	0.0	0.0	0.0	0.010049072063253749	0.0
34-35	0.0	5.431930845002026E-5	0.0	0.018142649022306768	0.0
36-37	0.0	1.0863861690004052E-4	0.0	0.030473132040461363	0.0
38-39	0.0	1.0863861690004052E-4	0.0	0.0538847539824201	0.0
40-41	0.0	1.0863861690004052E-4	0.0	0.0814789626750304	0.0
42-43	0.0	1.0863861690004052E-4	0.0	0.10499922323388916	0.0
44-45	0.0	1.0863861690004052E-4	0.0	0.13416869187155006	0.0
46-47	0.0	1.0863861690004052E-4	0.0	0.16551093284721174	0.0
48-49	0.0	1.0863861690004052E-4	0.0	0.2032085329115258	0.0
50-51	0.0	1.0863861690004052E-4	0.0	0.24405665286594103	0.0
52-53	0.0	1.0863861690004052E-4	0.0	0.28729482239215715	0.0
54-55	0.0	2.715965422501013E-4	0.0	0.332379848405674	0.0
56-57	0.0	3.259158507001216E-4	0.0	0.38604732515429396	0.0
58-59	0.0	3.259158507001216E-4	0.0	0.43928024743531385	0.0
60-61	0.0	3.259158507001216E-4	0.0	0.5057127616696886	0.0
62-63	0.0	3.259158507001216E-4	0.0	0.5765451398885151	0.0
64-65	0.0	3.259158507001216E-4	0.0	0.6428690155059897	0.0
66-67	0.0	3.259158507001216E-4	0.0	0.72380478509652	0.0
68-69	0.0	3.259158507001216E-4	0.0	0.8221770526995067	0.0
70-71	0.0	3.259158507001216E-4	0.0	0.9287515358784464	0.0
72-73	0.0	3.259158507001216E-4	0.0	1.0440171084093894	0.0
74-75	0.0	3.259158507001216E-4	0.0	1.1592283616318824	0.0
76-77	0.0	3.259158507001216E-4	0.0	1.270528624645974	0.0
78-79	0.0	3.259158507001216E-4	0.0	1.4031220565724734	0.0
80-81	0.0	3.259158507001216E-4	0.0	1.5629837813408831	0.0
82-83	0.0	3.259158507001216E-4	0.0	1.7353932663612475	0.0
84-85	0.0	3.259158507001216E-4	0.0	1.9107903133463626	0.0
86-87	0.0	3.259158507001216E-4	0.0	2.101451086005934	0.0
88-89	0.0	3.259158507001216E-4	0.0	2.307810138807561	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	845	0.0	59.64902	1
GTATCAA	1805	0.0	52.16055	1
AACCGTG	230	0.0	39.225285	7
ATCAACG	2455	0.0	38.296055	3
AAACCGT	225	0.0	37.986595	6
CCGTGCG	260	0.0	36.54741	9
TCAACGC	2575	0.0	36.511387	4
GTCTAAT	505	0.0	35.7805	1
GTATAAG	355	0.0	33.4862	1
ACCGTGC	320	0.0	32.6465	8
ACGCAGA	2955	0.0	32.137558	7
CGCAGAG	2960	0.0	32.085014	8
GCCCATA	1150	0.0	31.424614	1
CATAATA	1215	0.0	30.873877	4
CTAATGT	630	0.0	30.14809	3
GCGTAAG	80	4.5022485E-4	29.677025	7
TATCAAC	3190	0.0	29.621206	2
GGCGTAA	65	0.0058535603	29.220455	6
GTACATG	4545	0.0	29.189356	1
CCCATAA	1205	0.0	29.159834	2
>>END_MODULE