FastQCFastQC Report
Fri 10 Feb 2017
SRR3224534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920483
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG26400.286805948616107No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT22690.24650102174619193No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA21250.23085706091258612No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA20220.21966728337188193No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC20120.2185808972028815No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG19970.2169513179493809No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA19300.20967253061707822No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG18430.20022097094677468No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG18280.19859139169327408No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC17530.19044349542577105No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC16250.17653775246256584No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA15920.1729526781048645No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG15720.17077990576686372No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT14790.16067651439515993No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT14420.15665688556985843No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA13670.1485089893023554No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA13210.14351161292495354No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG13100.14231658813905307No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG12950.14068700888555247No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT12260.13319094431944967No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG12120.13167000368284912No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC12080.13123544921524896No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT11420.12406530049984628No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC11380.12363074603224612No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT11260.12232708262944562No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG11240.12210980539564555No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA11180.1214579736942453No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC11140.12102341922664514No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG11020.11971975582384464No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT10780.11711242901824369No Hit
GATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATA10100.10972500306904093No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA10010.10874725551694056No Hit
GTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATC9370.10179438403533797No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG9290.10092527510013766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.063.397661
GTATCAA19300.053.8686261
CCGTGCG2900.042.5786369
AACCGTG2900.040.9409987
AAACCGT3000.039.5762946
ATCAACG27000.038.1691363
GTCTAAT6000.038.152831
ACCGTGC3700.035.9395568
TCAACGC29150.035.3539164
CTAATGT6800.032.8250473
TCTAATG7400.032.088892
CGCAGAG32350.031.5631668
ACGCAGA33650.030.6260537
CAACGCA34050.030.2662755
TATCAAC35300.029.5981352
AACGCAG34950.029.4868876
TCGCTAA650.005848556329.225579
TAATGTT7850.029.0394214
GTTATTA8150.027.3877688
CTCGCTA700.00839799727.1380318
Produced by FastQC (version 0.11.5)