##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224534_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	920483
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.15240694287673	34.0	33.0	34.0	31.0	34.0
2	33.277534729049854	34.0	34.0	34.0	31.0	34.0
3	33.395784604387046	34.0	34.0	34.0	31.0	34.0
4	36.61837752571204	37.0	37.0	37.0	35.0	37.0
5	36.64611405099279	37.0	37.0	37.0	35.0	37.0
6	36.68377362754119	37.0	37.0	37.0	36.0	37.0
7	36.66045326203743	37.0	37.0	37.0	35.0	37.0
8	36.6471233037438	37.0	37.0	37.0	35.0	37.0
9	38.48848810896019	39.0	39.0	39.0	37.0	39.0
10-11	38.50905285594628	39.0	39.0	39.0	37.0	39.0
12-13	38.48233318811971	39.0	39.0	39.0	37.0	39.0
14-15	40.05985064362949	41.0	40.0	41.0	38.0	41.0
16-17	40.06435751665158	41.0	40.0	41.0	38.0	41.0
18-19	40.053072680321094	41.0	40.0	41.0	38.0	41.0
20-21	40.04707202631662	41.0	40.0	41.0	38.0	41.0
22-23	39.97268173339431	41.0	40.0	41.0	38.0	41.0
24-25	39.92055094988174	41.0	40.0	41.0	38.0	41.0
26-27	39.83223318627286	41.0	40.0	41.0	38.0	41.0
28-29	39.81004048961252	41.0	40.0	41.0	37.5	41.0
30-31	39.73849815803225	41.0	40.0	41.0	37.0	41.0
32-33	39.685987139360535	41.0	40.0	41.0	37.0	41.0
34-35	39.59683611756002	41.0	40.0	41.0	36.5	41.0
36-37	39.45484816123709	41.0	39.5	41.0	36.0	41.0
38-39	39.31104648320502	41.0	39.0	41.0	35.5	41.0
40-41	39.151324902252405	41.0	39.0	41.0	35.0	41.0
42-43	39.01500082022156	41.0	39.0	41.0	35.0	41.0
44-45	38.71532499785439	40.0	38.0	41.0	35.0	41.0
46-47	38.444954442396	40.0	37.5	41.0	35.0	41.0
48-49	38.28705092869721	40.0	37.0	41.0	35.0	41.0
50-51	38.05812057365536	40.0	36.5	41.0	34.5	41.0
52-53	37.73997998876677	39.5	36.0	41.0	34.0	41.0
54-55	37.48901174709364	39.0	35.0	41.0	34.0	41.0
56-57	37.18756620165718	38.5	35.0	41.0	34.0	41.0
58-59	36.85931787985221	37.5	35.0	41.0	33.0	41.0
60-61	36.55695216533059	37.0	35.0	40.0	33.0	41.0
62-63	36.318635433788565	36.0	35.0	40.0	33.0	41.0
64-65	36.024192733597474	36.0	35.0	39.5	33.0	41.0
66-67	35.73949057179763	35.0	35.0	39.0	32.0	41.0
68-69	35.467435574584215	35.0	35.0	39.0	32.0	41.0
70-71	35.194025310624966	35.0	35.0	38.5	32.0	40.5
72-73	34.918035422707426	35.0	35.0	37.0	32.0	40.0
74-75	34.642544729234544	35.0	35.0	37.0	31.0	39.0
76-77	32.892678083136786	34.5	32.0	35.0	28.5	38.0
78-79	34.25013987221926	35.0	34.0	36.0	31.5	38.5
80-81	34.24221794427491	35.0	35.0	36.0	32.0	37.0
82-83	34.08934983046944	35.0	35.0	35.0	32.0	37.0
84-85	33.945396601566785	35.0	35.0	35.0	32.0	36.5
86-87	33.80775147395444	35.0	34.5	35.0	31.0	36.0
88-89	33.70317648451954	35.0	34.0	35.0	31.0	36.0
90-91	33.582310048094314	35.0	34.0	35.0	31.0	36.0
92-93	33.504233646900595	35.0	34.0	35.0	31.0	35.5
94-95	33.42172967887511	35.0	34.0	35.0	31.0	35.0
96-97	33.392856793661586	35.0	34.0	35.0	31.0	35.0
98-99	33.37955616779452	35.0	34.0	35.0	31.0	35.0
100-101	32.480753039436905	34.5	32.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	6.0
14	22.0
15	54.0
16	144.0
17	325.0
18	544.0
19	919.0
20	1260.0
21	1877.0
22	2543.0
23	2516.0
24	2341.0
25	2853.0
26	3811.0
27	5220.0
28	6787.0
29	7557.0
30	7988.0
31	8707.0
32	10515.0
33	14136.0
34	25812.0
35	54994.0
36	158501.0
37	300142.0
38	244236.0
39	56661.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.989713000911365	18.01671023406334	12.874520440294477	24.11905632473082
2	16.635070935584906	19.27509796487279	37.89228046579893	26.197550633743372
3	20.233399204548046	26.25186994219339	27.631037183739405	25.883693669519154
4	13.504323273759535	16.33403332815489	38.106189902475116	32.05545349561046
5	14.26587997822882	37.912921803009944	33.80670800003911	14.014490218722129
6	30.492252437035773	35.614563223872686	19.38319338868833	14.509990950403212
7	27.98324357972934	30.257701663148584	21.790625139193228	19.968429617928848
8	27.385731186779118	32.474364002377015	19.849578971040206	20.290325839803668
9	27.837885110317085	14.751929150239604	19.407962993341542	38.00222274610178
10-11	27.71990357236364	23.548723876486584	26.50108692936208	22.230285621787694
12-13	27.98595954515184	22.028054836428264	26.550300222817803	23.43568539560209
14-15	24.891823097221785	23.91244596586792	24.04704921220707	27.148681724703227
16-17	24.378777228911343	26.228404000942984	25.193240939810945	24.199577830334725
18-19	24.45797478063147	25.6009616690368	26.188750905774466	23.75231264455726
20-21	25.770688209143966	23.926262064410153	25.795295042999495	24.50775468344639
22-23	26.096436550655344	24.67243654196414	24.833712758855388	24.39741414852513
24-25	25.549522477921872	24.281544105744715	25.266518265993543	24.902415150339877
26-27	24.85071396017989	25.75867944934324	25.535585565765977	23.855021024710894
28-29	24.75275653512579	25.608154281225794	25.971889805953396	23.667199377695017
30-31	24.623753222527984	24.415436411497133	26.155954601601568	24.80485576437332
32-33	24.80977357514867	24.877564145741037	26.36825054699603	23.94441173211426
34-35	25.692866113605056	24.282635053212985	25.272909396687716	24.751589436494246
36-37	24.295124907044215	24.478184655681996	26.27075786192732	24.955932575346466
38-39	24.680140752192056	24.864391846454524	25.186885580722297	25.268581820631127
40-41	25.031097804087636	24.755155717161532	25.751969346527854	24.461777132222977
42-43	24.974334126757366	24.855211883326472	26.005423239755647	24.16503075016051
44-45	25.21158910753398	24.255554010360534	25.7195116121406	24.813345269964884
46-47	24.858731264055525	24.261094522170563	25.326283894304076	25.553890319469836
48-49	24.495287759037453	24.221461745294548	26.09728672696162	25.185963768706376
50-51	24.98449183743752	24.60501714860568	25.352722429420204	25.057768584536593
52-53	25.281537281554662	24.87055694733846	24.635788641168432	25.212117129938445
54-55	25.294437811453335	23.881158044200706	25.262715335318525	25.561688809027434
56-57	25.4530718053827	24.628875236782676	24.70107120565095	25.21698175218367
58-59	25.716658071787258	24.656526339184552	25.245905024499482	24.38091056452871
60-61	25.091406610400004	25.209804591711706	24.87297619054088	24.825812607347412
62-63	24.796709988125798	24.452814446328723	25.648654021855915	25.10182154368956
64-65	25.31171135154044	24.276168055249254	25.050272519970495	25.361848073239813
66-67	24.743205469302527	24.460093233661024	24.930498444838197	25.86620285219825
68-69	25.193729813586994	24.58328942522567	25.537625355384076	24.685355405803257
70-71	25.757509915989758	24.277580357268956	25.254458800434122	24.710450926307168
72-73	24.998777815559876	24.540377171550155	25.560982658017583	24.899862354872386
74-75	25.074335973613852	24.215982261486634	25.68624298330333	25.023438781596184
76-77	25.128614891896135	23.535262289641118	25.29651633032965	26.0396064881331
78-79	25.088465721366386	23.53351158664643	25.230684486203476	26.14733820578371
80-81	24.55065814383394	24.768273941676142	25.258968774903458	25.422099139586464
82-83	24.472582090768352	24.763410195822807	25.6522990847117	25.111708628697137
84-85	24.795021635850652	24.093200921710054	25.90392009238829	25.207857350051004
86-87	24.549502815369756	25.349137355062506	25.218064863772604	24.88329496579513
88-89	25.14734112417068	24.163183893673214	24.517400104075797	26.172074878080316
90-91	25.0809086099363	24.37215027328044	24.790789183504746	25.75615193327851
92-93	25.084724587376726	24.564942842476636	25.340405711135194	25.009926859011443
94-95	25.125830678024474	24.10017349587119	25.107090516609215	25.666905309495124
96-97	25.320076525041745	24.813440335128405	25.25565382522002	24.61082931460983
98-99	23.271532445466132	25.422142505619334	25.792382912014673	25.513942136899864
100-101	24.60590406024405	24.57345341702012	25.18104686753452	25.639595655201315
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	2.5
2	3.0
3	5.0
4	3.5
5	5.5
6	6.5
7	20.0
8	40.5
9	47.0
10	47.0
11	46.0
12	47.0
13	54.5
14	67.5
15	74.5
16	74.5
17	89.0
18	105.5
19	120.0
20	145.5
21	184.5
22	221.0
23	268.5
24	377.5
25	541.5
26	752.5
27	999.0
28	1264.5
29	1740.0
30	2631.0
31	3840.0
32	5366.5
33	7069.0
34	8692.5
35	10608.0
36	13143.5
37	15105.5
38	15988.0
39	16843.0
40	17560.0
41	17937.0
42	18760.5
43	20415.0
44	22596.0
45	25723.5
46	29381.5
47	32166.5
48	35121.5
49	41111.5
50	48472.5
51	49006.5
52	45538.0
53	47274.5
54	51787.5
55	51681.5
56	43877.5
57	37593.0
58	36572.0
59	35601.0
60	29865.0
61	22120.5
62	17710.5
63	12388.0
64	7179.0
65	4522.0
66	3403.5
67	2811.0
68	2029.0
69	1294.0
70	749.0
71	475.0
72	340.5
73	228.0
74	162.0
75	126.0
76	97.5
77	66.0
78	37.5
79	22.0
80	9.5
81	8.0
82	6.5
83	5.0
84	3.5
85	1.0
86	0.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.46475600309837334
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.604703183002837E-4
22-23	0.0013579827112505064
24-25	2.1727723380008106E-4
26-27	0.00200981441265075
28-29	0.003693712974601378
30-31	0.0010863861690004052
32-33	1.0863861690004053E-4
34-35	0.0033677971239012565
36-37	0.00200981441265075
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.007278787332302716
46-47	0.0753408808201781
48-49	8.691089352003242E-4
50-51	0.0
52-53	1.0863861690004053E-4
54-55	0.0
56-57	0.007767661108352897
58-59	0.03927286000936465
60-61	0.030581770657361406
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.715965422501013E-4
78-79	0.007604703183002837
80-81	0.005812166004152168
82-83	8.691089352003242E-4
84-85	0.0021727723380008104
86-87	0.0
88-89	0.0
90-91	0.0
92-93	5.4319308450020265E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0678448162540753
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	920483.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.81164294785591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.33702916976637	29.82718392607053
2	15.622748787812025	13.0642558836009
3	5.610640027121561	7.03770232568866
4	2.5753136082605854	4.307123722693842
5	1.3650146334659201	2.853675223653773
6	0.8556061080730373	2.1464578256852667
7	0.5129675677893413	1.5013611751766607
8	0.35629225314016866	1.191773157870706
9	0.2622857360915338	0.9869937792997299
>10	1.1961943836367195	9.189796098377883
>50	0.1307324827209084	3.8694347323529126
>100	0.14814523443706895	13.613537989756963
>500	0.018713082243314763	5.192257332949693
>1k	0.008316925441473227	5.218446826822462
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2640	0.286805948616107	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	2269	0.24650102174619193	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	2125	0.23085706091258612	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	2022	0.21966728337188193	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	2012	0.2185808972028815	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1997	0.2169513179493809	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1930	0.20967253061707822	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1843	0.20022097094677468	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1828	0.19859139169327408	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1753	0.19044349542577105	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1625	0.17653775246256584	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1592	0.1729526781048645	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1572	0.17077990576686372	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	1479	0.16067651439515993	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1442	0.15665688556985843	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	1367	0.1485089893023554	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1321	0.14351161292495354	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	1310	0.14231658813905307	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	1295	0.14068700888555247	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	1226	0.13319094431944967	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	1212	0.13167000368284912	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC	1208	0.13123544921524896	No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT	1142	0.12406530049984628	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1138	0.12363074603224612	No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT	1126	0.12232708262944562	No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG	1124	0.12210980539564555	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1118	0.1214579736942453	No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC	1114	0.12102341922664514	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	1102	0.11971975582384464	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	1078	0.11711242901824369	No Hit
GATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATA	1010	0.10972500306904093	No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA	1001	0.10874725551694056	No Hit
GTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATC	937	0.10179438403533797	No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG	929	0.10092527510013766	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	5.431930845002026E-5	0.0
16-17	0.0	0.0	0.0	1.0863861690004052E-4	0.0
18-19	0.0	0.0	0.0	2.715965422501013E-4	0.0
20-21	0.0	0.0	0.0	6.518317014002431E-4	0.0
22-23	0.0	0.0	0.0	7.604703183002837E-4	0.0
24-25	0.0	0.0	0.0	0.001032066860550385	0.0
26-27	0.0	0.0	0.0	0.0014123020197005268	0.0
28-29	0.0	0.0	0.0	0.0017382178704006485	0.0
30-31	0.0	0.0	0.0	0.0038566708999514384	0.0
32-33	0.0	0.0	0.0	0.009940433446353708	0.0
34-35	0.0	0.0	0.0	0.018142649022306768	0.0
36-37	0.0	0.0	0.0	0.030473132040461363	0.0
38-39	0.0	0.0	0.0	0.053993392599320136	0.0
40-41	0.0	0.0	0.0	0.08185919783418052	0.0
42-43	0.0	0.0	0.0	0.10575969355218945	0.0
44-45	0.0	0.0	0.0	0.1348748428814003	0.0
46-47	0.0	0.0	0.0	0.16665163832466218	0.0
48-49	0.0	0.0	0.0	0.2048381121650264	0.0
50-51	0.0	0.0	0.0	0.24622942520394184	0.0
52-53	0.0	0.0	0.0	0.2897935105808581	0.0
54-55	0.0	0.0	0.0	0.3356390069126752	0.0
56-57	0.0	0.0	0.0	0.38968671882044537	0.0
58-59	0.0	0.0	0.0	0.44351715349441545	0.0
60-61	0.0	0.0	0.0	0.5110360538977906	0.0
62-63	0.0	0.0	0.0	0.5831720955194175	0.0
64-65	0.0	5.431930845002026E-5	0.0	0.6504193993805426	0.0
66-67	0.0	1.0863861690004052E-4	0.0	0.7335822606175236	0.0
68-69	0.0	1.0863861690004052E-4	0.0	0.8326063599219106	0.0
70-71	0.0	1.0863861690004052E-4	0.0	0.941027699588151	0.0
72-73	0.0	1.0863861690004052E-4	0.0	1.0581401286063947	0.0
74-75	0.0	1.0863861690004052E-4	0.0	1.1749266417739381	0.0
76-77	0.0	1.0863861690004052E-4	0.0	1.2886169543598307	0.0
78-79	0.0	2.1727723380008106E-4	0.0	1.4225140496891306	0.0
80-81	0.0	3.259158507001216E-4	0.0	1.5836794378603407	0.0
82-83	0.0	3.259158507001216E-4	0.0	1.7578814600595556	0.0
84-85	0.0	3.259158507001216E-4	0.0	1.9361031110840723	0.0
86-87	0.0	3.259158507001216E-4	0.0	2.131598302195695	0.0
88-89	0.0	3.259158507001216E-4	0.0	2.342520176907124	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	835	0.0	63.39766	1
GTATCAA	1930	0.0	53.868626	1
CCGTGCG	290	0.0	42.578636	9
AACCGTG	290	0.0	40.940998	7
AAACCGT	300	0.0	39.576294	6
ATCAACG	2700	0.0	38.169136	3
GTCTAAT	600	0.0	38.15283	1
ACCGTGC	370	0.0	35.939556	8
TCAACGC	2915	0.0	35.353916	4
CTAATGT	680	0.0	32.825047	3
TCTAATG	740	0.0	32.08889	2
CGCAGAG	3235	0.0	31.563166	8
ACGCAGA	3365	0.0	30.626053	7
CAACGCA	3405	0.0	30.266275	5
TATCAAC	3530	0.0	29.598135	2
AACGCAG	3495	0.0	29.486887	6
TCGCTAA	65	0.0058485563	29.22557	9
TAATGTT	785	0.0	29.039421	4
GTTATTA	815	0.0	27.387768	8
CTCGCTA	70	0.008397997	27.138031	8
>>END_MODULE