##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224533_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	894882
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29480870103544	34.0	31.0	34.0	31.0	34.0
2	32.46196593517357	34.0	31.0	34.0	31.0	34.0
3	32.600952974805615	34.0	31.0	34.0	31.0	34.0
4	35.88754606752622	37.0	37.0	37.0	35.0	37.0
5	35.90041256836097	37.0	37.0	37.0	35.0	37.0
6	35.996629723248425	37.0	37.0	37.0	35.0	37.0
7	35.96448023314806	37.0	37.0	37.0	35.0	37.0
8	36.00259810790696	37.0	37.0	37.0	35.0	37.0
9	37.74092338431212	39.0	39.0	39.0	35.0	39.0
10-11	37.81924711861452	39.0	39.0	39.0	36.0	39.0
12-13	37.80393225028551	39.0	39.0	39.0	35.0	39.0
14-15	39.30241249684316	41.0	40.0	41.0	37.0	41.0
16-17	39.13075411059782	41.0	40.0	41.0	36.0	41.0
18-19	39.211103251601884	41.0	40.0	41.0	36.0	41.0
20-21	39.27477309857613	41.0	40.0	41.0	36.0	41.0
22-23	39.22958725284451	41.0	40.0	41.0	36.0	41.0
24-25	39.16253651319391	41.0	40.0	41.0	36.0	41.0
26-27	38.98320001966739	41.0	39.0	41.0	35.0	41.0
28-29	38.91596378069958	41.0	39.0	41.0	35.0	41.0
30-31	38.84751620884094	41.0	39.0	41.0	35.0	41.0
32-33	38.650988063230685	41.0	39.0	41.0	35.0	41.0
34-35	38.646282973621105	41.0	39.0	41.0	35.0	41.0
36-37	38.619415744198676	40.5	39.0	41.0	35.0	41.0
38-39	38.483787247927665	40.0	38.0	41.0	35.0	41.0
40-41	38.37016221133065	40.0	38.0	41.0	34.0	41.0
42-43	38.2180678569912	40.0	38.0	41.0	34.0	41.0
44-45	38.00732163570169	40.0	37.5	41.0	33.5	41.0
46-47	37.806010736611086	40.0	37.0	41.0	33.0	41.0
48-49	37.560490098135844	40.0	36.5	41.0	33.0	41.0
50-51	36.22286960738958	38.5	35.0	40.0	31.0	40.5
52-53	36.505390654857294	39.0	35.0	40.0	31.5	40.5
54-55	36.89941578889731	39.0	35.0	41.0	32.0	41.0
56-57	36.71469981517116	39.0	35.0	41.0	32.0	41.0
58-59	36.53415031255518	38.0	35.0	41.0	32.0	41.0
60-61	36.2726163896469	37.0	35.0	41.0	31.0	41.0
62-63	35.99955357242631	37.0	35.0	40.5	31.0	41.0
64-65	35.749364161978896	36.0	35.0	40.0	31.0	41.0
66-67	35.540613734548245	36.0	35.0	40.0	31.0	41.0
68-69	35.274992680599226	35.0	35.0	39.5	30.0	41.0
70-71	35.024019367916665	35.0	35.0	39.0	30.0	41.0
72-73	34.7384437277764	35.0	34.5	39.0	29.5	41.0
74-75	34.49882889587677	35.0	34.0	37.5	29.0	40.5
76-77	34.21131892249481	35.0	34.0	37.0	29.0	39.5
78-79	34.00324903171591	35.0	34.0	37.0	29.0	39.0
80-81	33.72312662451586	35.0	34.0	36.0	29.0	39.0
82-83	33.48138078539964	35.0	34.0	36.0	29.0	37.0
84-85	33.2604175746076	35.0	34.0	35.5	29.0	37.0
86-87	33.053893697716575	35.0	34.0	35.0	28.0	37.0
88-89	32.913310358237176	35.0	34.0	35.0	27.5	36.0
90-91	32.73824873000015	35.0	33.0	35.0	27.0	36.0
92-93	32.60382597929112	35.0	33.0	35.0	27.0	36.0
94-95	32.47305231304239	35.0	33.0	35.0	27.0	35.5
96-97	32.34915776605183	35.0	33.0	35.0	26.0	35.0
98-99	32.319066647893244	35.0	33.0	35.0	26.0	35.0
100-101	31.079010417015873	34.0	31.0	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	143.0
8	398.0
9	309.0
10	387.0
11	780.0
12	1212.0
13	1497.0
14	1552.0
15	1597.0
16	1832.0
17	2032.0
18	2269.0
19	2583.0
20	3192.0
21	4120.0
22	5050.0
23	4901.0
24	4806.0
25	5032.0
26	5179.0
27	5370.0
28	5780.0
29	6485.0
30	8062.0
31	10660.0
32	13918.0
33	20449.0
34	35362.0
35	66440.0
36	144355.0
37	224597.0
38	224295.0
39	80229.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.424929967913386	19.530754242495124	13.329766910384668	23.71454887920682
2	16.614853388163773	20.072076703021636	37.769421598426604	25.543648310387983
3	20.229639728231717	26.940707133917396	28.100862685499735	24.728790452351156
4	13.526699609557461	16.470774917810395	38.004898969920056	31.99762650271209
5	13.91881834699994	39.11867709932706	33.34998357325323	13.612520980419765
6	29.556634282508753	36.89290878573935	18.947526042539685	14.60293088921221
7	26.77861438714825	31.17617741780481	22.577166598501254	19.46804159654569
8	26.0389637963441	33.95676748442811	20.660489315909807	19.343779403317978
9	27.123129082940544	15.063773771290517	20.241327906919572	37.57176923884937
10-11	26.55702092566394	24.717839893974848	26.939808823956678	21.78533035640453
12-13	26.644853734905833	22.740260727112624	27.339638075187565	23.275247462793978
14-15	24.059464103161734	24.698495846824656	24.52047467096824	26.72156537904537
16-17	23.650802881179846	27.28487604239239	25.765278450309044	23.299042626118716
18-19	23.565821028235078	26.641383022066183	26.471204644947548	23.32159130475119
20-21	25.077722813783854	24.739554512171264	26.056398647326795	24.12632402671809
22-23	25.272523249111156	25.82179101323241	25.156508325398658	23.749177412257776
24-25	24.313434679127845	25.680334359532615	25.730349699432953	24.275881261906584
26-27	23.81945329104843	26.898015604291963	26.04091936143536	23.241611743224247
28-29	23.500137448289273	26.665917964603153	26.557300292105552	23.276644295002022
30-31	23.70608638904347	25.613712198926784	26.509696250455367	24.170505161574376
32-33	23.593054726768443	26.03650537165794	26.663571286493635	23.706868615079976
34-35	24.377236328365083	25.37183673378166	26.096569156603888	24.154357781249374
36-37	23.394986592986776	25.554247826879077	26.968550801905934	24.082214778228217
38-39	23.580824578440893	26.017592109365655	25.847839764514717	24.55374354767873
40-41	24.198283472838416	25.48668462165998	26.655733491277672	23.65929841422393
42-43	24.044579314290758	25.980704125642774	26.427368918634258	23.54734764143221
44-45	24.073199666288737	25.605075182823146	26.089492956691856	24.232232194196264
46-47	24.103177849146594	25.33144034632499	25.984487340230334	24.580894464298087
48-49	23.386714672995993	25.716574922727244	26.51690390464888	24.379806499627886
50-51	23.525783287628986	26.043154292968236	26.058295954103443	24.372766465299335
52-53	23.898458120735473	26.042316193643405	25.405304833486426	24.653920852134696
54-55	24.04909250605108	25.407148652001045	25.888664650758425	24.655094191189452
56-57	24.12576413748636	25.78302266836447	25.754024415523176	24.337188778625997
58-59	24.155801772471204	26.003490401396164	26.069040206674682	23.771667619457954
60-61	23.874926094114464	26.273220977759497	25.77211392627182	24.079739001854215
62-63	23.49907027616738	25.79088004354919	26.517724554713425	24.192325125570004
64-65	24.608992214781907	25.07173390619838	25.647226261010243	24.672047618009472
66-67	23.557452116251024	25.93400833110731	25.373776571191627	25.13476298145004
68-69	23.649039761666902	25.97247458324114	26.821972058885972	23.55651359620598
70-71	24.34030408478436	25.320265688660626	26.27279350797088	24.066636718584125
72-73	23.777436578230425	25.74087980314723	26.547466593360912	23.934217025261432
74-75	23.895273343301128	25.312611048160537	26.595014761722773	24.19710084681556
76-77	23.620823751064385	25.171586868436286	25.83698185906075	25.370607521438583
78-79	24.021812203366718	24.400865737483493	26.477361568341678	25.099960490808105
80-81	23.62790245222376	25.995707504264264	25.724337690494174	24.6520523530178
82-83	23.22404860345564	26.208825364313487	26.223192184449847	24.343933847781024
84-85	23.667433288528336	24.931716627497227	26.868242572943185	24.53260751103125
86-87	23.44003063668967	26.357783983384625	26.130133252828163	24.07205212709754
88-89	23.862464529305207	25.31125063088512	25.74911145106092	25.077173388748754
90-91	23.48343133508105	25.253608855692704	25.92922865808006	25.333731151146182
92-93	23.403575311864774	25.448422600655523	26.545522814208717	24.602479273270987
94-95	23.742012913434397	24.798856162041478	26.527240462988416	24.931890461535712
96-97	24.08149901327773	25.552698568079368	26.336600803234393	24.029201615408514
98-99	22.736768704955377	25.954810524133777	26.564782961671245	24.743637809239598
100-101	23.697167548954393	25.217534699901055	26.044071529590646	25.04122622155391
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	2.0
3	3.5
4	5.5
5	4.5
6	5.0
7	9.5
8	22.0
9	30.5
10	25.5
11	28.0
12	36.0
13	43.0
14	45.0
15	44.5
16	48.0
17	43.5
18	49.5
19	70.5
20	85.0
21	95.5
22	119.5
23	143.0
24	158.0
25	204.5
26	288.0
27	409.0
28	528.5
29	700.0
30	996.0
31	1761.0
32	3606.0
33	6768.5
34	11301.0
35	18181.0
36	26915.0
37	34315.5
38	37212.5
39	35644.5
40	32777.0
41	29386.0
42	25478.0
43	22905.5
44	21597.5
45	22101.5
46	24484.5
47	25042.0
48	26611.5
49	31499.5
50	36729.0
51	37783.0
52	37526.0
53	39775.5
54	42878.0
55	43339.5
56	36104.0
57	30346.5
58	29338.5
59	28346.5
60	24399.5
61	18870.0
62	15226.5
63	10620.0
64	6274.5
65	3929.5
66	2981.5
67	2683.5
68	2109.5
69	1301.0
70	718.5
71	436.5
72	327.0
73	257.5
74	194.0
75	151.5
76	111.0
77	63.0
78	48.5
79	37.5
80	27.5
81	19.0
82	9.5
83	10.5
84	9.5
85	8.5
86	7.0
87	6.5
88	5.5
89	4.0
90	5.5
91	4.0
92	3.5
93	3.5
94	3.0
95	3.5
96	3.5
97	4.5
98	3.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15231058396526023
2	2.2349315328724906E-4
3	2.2349315328724906E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.036373510697499785
16-17	0.07431147346801031
18-19	0.025981079069642702
20-21	0.039893527861773956
22-23	0.06710381927449653
24-25	0.017488339244727237
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02246106190536853
38-39	0.005475582255537602
40-41	0.005810821985468475
42-43	0.0030730308576996743
44-45	0.010783544646109767
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	5.5873288321812265E-5
58-59	0.015923887171716493
60-61	0.018214691992910797
62-63	0.02922172979230781
64-65	0.04810690124508035
66-67	0.018214691992910797
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.017153099514796363
80-81	0.05928155890944281
82-83	0.05140342525606728
84-85	0.029556969522238685
86-87	0.05916981233279918
88-89	0.03413857916462729
90-91	0.0
92-93	0.002570171262803364
94-95	0.0
96-97	0.0
98-99	6.146061715399349E-4
100-101	0.04771578822682767
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	894882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.04050298510802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.69036459165994	27.20746886873966
2	15.264808826665707	11.918916291290916
3	5.9044100108296105	6.915334099592853
4	2.8695987411137307	4.481223128740512
5	1.6068922786116293	3.1366941399942174
6	1.0350080676875155	2.4244341333699198
7	0.6781452620115431	1.8532592488128958
8	0.4721570872784119	1.4746600140266197
9	0.35497604782713865	1.2472599109353528
>10	1.7636106477753333	12.523916211667613
>50	0.16343796549591116	4.504749686277466
>100	0.17332532830489716	14.390721566011337
>500	0.017448858554028977	4.602496791665694
>1k	0.005816286184676326	3.3188659088749217
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2853	0.31881298316426077	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2212	0.24718342753569747	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2105	0.2352265438348296	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1739	0.19432729678326305	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1675	0.18717551587807107	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1567	0.17510688560055962	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1551	0.17331894037426163	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1413	0.15789791279744145	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1366	0.1526458236951911	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1283	0.14337085783377027	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1280	0.14303561810383938	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	1231	0.1375600358483018	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1223	0.1366660632351528	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1211	0.1353251043154293	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1210	0.13521335773878568	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1190	0.13297842620591319	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	1063	0.11878661097217287	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC	1044	0.116663426015944	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1011	0.11297578898670439	No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT	1005	0.11230530952684266	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	974	0.10884116565089029	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	966	0.10794719303774129	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	948	0.10593575465815605	No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG	919	0.10269510393549094	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	897	0.1002366792493312	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	5.587328832181226E-5	0.0
16-17	0.0	0.0	0.0	1.1174657664362452E-4	0.0
18-19	0.0	0.0	0.0	2.2349315328724903E-4	0.0
20-21	0.0	0.0	0.0	4.469863065744981E-4	0.0
22-23	0.0	0.0	0.0	7.263527481835594E-4	0.0
24-25	0.0	0.0	0.0	0.0015644520730107433	0.0
26-27	0.0	0.0	0.0	0.002179058244550678	0.0
28-29	0.0	0.0	0.0	0.0035758904525959845	0.0
30-31	0.0	0.0	0.0	0.008772106266524525	5.587328832181226E-5
32-33	0.0	0.0	0.0	0.0205054968141051	1.1174657664362452E-4
34-35	0.0	0.0	0.0	0.037043990157361525	1.1174657664362452E-4
36-37	0.0	0.0	0.0	0.06453364801169316	1.1174657664362452E-4
38-39	0.0	0.0	0.0	0.11414912804146246	1.1174657664362452E-4
40-41	0.0	0.0	0.0	0.16801097798368947	1.1174657664362452E-4
42-43	0.0	0.0	0.0	0.22528109851354705	1.1174657664362452E-4
44-45	0.0	0.0	0.0	0.28607123620767877	1.1174657664362452E-4
46-47	0.0	0.0	0.0	0.3555217335916914	1.1174657664362452E-4
48-49	0.0	0.0	0.0	0.4340237036838377	1.1174657664362452E-4
50-51	0.0	0.0	0.0	0.5250971636483917	1.1174657664362452E-4
52-53	0.0	0.0	0.0	0.6111420276639825	1.1174657664362452E-4
54-55	0.0	0.0	0.0	0.7027742205117546	1.1174657664362452E-4
56-57	0.0	0.0	0.0	0.8042401121041657	1.1174657664362452E-4
58-59	0.0	0.0	0.0	0.9264908669522909	1.1174657664362452E-4
60-61	0.0	0.0	0.0	1.0622629575742946	1.1174657664362452E-4
62-63	0.0	0.0	0.0	1.1918331131925775	1.1174657664362452E-4
64-65	0.0	0.0	0.0	1.322297241424009	1.1174657664362452E-4
66-67	0.0	0.0	0.0	1.4805303939513812	1.1174657664362452E-4
68-69	0.0	0.0	0.0	1.6636830330702819	1.1174657664362452E-4
70-71	0.0	0.0	0.0	1.8657767169302768	1.1174657664362452E-4
72-73	0.0	0.0	0.0	2.074128209082315	1.1174657664362452E-4
74-75	0.0	0.0	0.0	2.2872289307417066	1.1174657664362452E-4
76-77	0.0	0.0	0.0	2.506755080558107	1.1174657664362452E-4
78-79	0.0	0.0	0.0	2.766509997966212	1.1174657664362452E-4
80-81	0.0	0.0	0.0	3.067499402155815	1.1174657664362452E-4
82-83	0.0	0.0	0.0	3.3905028819442116	1.1174657664362452E-4
84-85	0.0	0.0	0.0	3.7118860363712756	1.1174657664362452E-4
86-87	0.0	0.0	0.0	4.046399413553965	1.1174657664362452E-4
88-89	0.0	0.0	0.0	4.416448202109328	1.1174657664362452E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	905	0.0	54.635487	1
GTATCAA	1800	0.0	47.27925	1
CCGTGCG	205	0.0	46.34749	9
AAACCGT	255	0.0	37.247253	6
ACCGTGC	285	0.0	34.99477	8
AACCGTG	285	0.0	34.992817	7
ATCAACG	2435	0.0	34.325603	3
TCAACGC	2545	0.0	32.655376	4
GTACATG	5115	0.0	29.557762	1
ACCGACC	100	4.9569462E-5	28.495743	8
CGCAGAG	3040	0.0	28.277025	8
ACATGGG	5330	0.0	27.710072	3
ATGCGGG	275	0.0	27.632233	8
ACGCAGA	3100	0.0	27.574982	7
CCCTACG	70	0.008353312	27.167648	1
TACATGG	5545	0.0	26.97823	2
TATCAAC	3185	0.0	26.689968	2
CGGCTTA	185	0.0	25.683321	16-17
GCGCCTC	130	1.0033806E-5	25.571672	6
GCAGAGT	3370	0.0	25.374218	9
>>END_MODULE