##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224533_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	894882
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.169686059167574	34.0	33.0	34.0	31.0	34.0
2	33.294365067126165	34.0	33.0	34.0	31.0	34.0
3	33.409462923603336	34.0	34.0	34.0	31.0	34.0
4	36.62809174840929	37.0	37.0	37.0	35.0	37.0
5	36.66123801797332	37.0	37.0	37.0	35.0	37.0
6	36.70518682910149	37.0	37.0	37.0	36.0	37.0
7	36.68539427544637	37.0	37.0	37.0	36.0	37.0
8	36.675268918136695	37.0	37.0	37.0	36.0	37.0
9	38.52254822423515	39.0	39.0	39.0	37.0	39.0
10-11	38.55136822508443	39.0	39.0	39.0	37.5	39.0
12-13	38.527378470010575	39.0	39.0	39.0	37.0	39.0
14-15	40.12313131787208	41.0	40.0	41.0	38.0	41.0
16-17	40.13093849244929	41.0	40.0	41.0	38.0	41.0
18-19	40.12662004599489	41.0	40.0	41.0	38.0	41.0
20-21	40.125411506768486	41.0	40.0	41.0	38.0	41.0
22-23	40.05553190253016	41.0	40.0	41.0	38.0	41.0
24-25	40.012473711617844	41.0	40.0	41.0	38.0	41.0
26-27	39.9349405843452	41.0	40.0	41.0	38.0	41.0
28-29	39.91062229433601	41.0	40.0	41.0	38.0	41.0
30-31	39.85698952487591	41.0	40.0	41.0	38.0	41.0
32-33	39.81429674526921	41.0	40.0	41.0	37.0	41.0
34-35	39.73415824656212	41.0	40.0	41.0	37.0	41.0
36-37	39.60841429372811	41.0	40.0	41.0	37.0	41.0
38-39	39.48463037584844	41.0	39.5	41.0	36.0	41.0
40-41	39.3402929101267	41.0	39.0	41.0	35.5	41.0
42-43	39.231484709715915	41.0	39.0	41.0	35.0	41.0
44-45	38.95867332229277	41.0	38.5	41.0	35.0	41.0
46-47	38.71135747506375	40.0	38.0	41.0	35.0	41.0
48-49	38.57556582879083	40.0	37.5	41.0	35.0	41.0
50-51	38.36952860824108	40.0	37.0	41.0	35.0	41.0
52-53	38.08081847662597	40.0	36.5	41.0	35.0	41.0
54-55	37.85906465880417	39.5	35.5	41.0	34.5	41.0
56-57	37.59831687306259	39.0	35.0	41.0	34.0	41.0
58-59	37.30572578284064	39.0	35.0	41.0	34.0	41.0
60-61	37.046597763727505	38.0	35.0	41.0	34.0	41.0
62-63	36.83529951434937	37.0	35.0	41.0	33.5	41.0
64-65	36.58339311775184	37.0	35.0	40.5	33.0	41.0
66-67	36.339704005667784	36.0	35.0	40.0	33.0	41.0
68-69	36.1121700961691	36.0	35.0	40.0	33.0	41.0
70-71	35.85721469422784	35.0	35.0	39.0	33.0	41.0
72-73	35.60085575528394	35.0	35.0	39.0	33.0	41.0
74-75	35.323495723458514	35.0	35.0	38.5	32.0	40.5
76-77	33.51798002418196	34.5	32.0	35.5	29.0	39.0
78-79	34.83347022288972	35.0	34.5	37.0	32.5	39.0
80-81	34.77373888400929	35.0	35.0	36.5	33.0	39.0
82-83	34.58008374288454	35.0	35.0	36.0	33.0	38.0
84-85	34.38639787145121	35.0	35.0	36.0	33.0	37.0
86-87	34.20480521454225	35.0	35.0	35.5	32.0	37.0
88-89	34.076275419552516	35.0	35.0	35.0	32.0	36.0
90-91	33.93326382696266	35.0	35.0	35.0	32.0	36.0
92-93	33.82757670843753	35.0	35.0	35.0	32.0	36.0
94-95	33.734690160266936	35.0	34.0	35.0	32.0	36.0
96-97	33.69373280499552	35.0	34.0	35.0	31.5	35.5
98-99	33.67861405190852	35.0	34.0	35.0	31.0	35.0
100-101	32.83836248801518	34.5	33.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	8.0
15	41.0
16	109.0
17	233.0
18	412.0
19	631.0
20	891.0
21	1343.0
22	1915.0
23	1879.0
24	1836.0
25	2289.0
26	3082.0
27	4277.0
28	5824.0
29	6662.0
30	7071.0
31	7561.0
32	8831.0
33	11831.0
34	21215.0
35	45878.0
36	136942.0
37	260886.0
38	260289.0
39	102939.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.79204068915573	19.771628745137008	13.484570344297175	22.951760221410087
2	16.438815396890316	20.133939446765048	38.46663582461151	24.96060933173312
3	19.87692232048471	27.652584363078038	28.380725056487893	24.089768259949356
4	13.463339300600527	16.688792488842104	38.78868945849844	31.05917875205893
5	13.80159618810078	38.35097811778536	34.289772282826114	13.557653411287745
6	29.37493434888622	36.685954125795355	19.406916219121626	14.532195306196794
7	26.815490757440646	30.972351662006837	23.119472734952765	19.092684845599756
8	26.104447290257266	33.56990083608788	20.683062124391817	19.64258974926303
9	27.023562883151076	15.31699151396497	20.605733493354432	37.05371210952953
10-11	26.607753871460147	24.631627410094293	27.351986071906687	21.408632646538873
12-13	26.811747247123087	23.026108470167017	27.449652579893215	22.712491702816685
14-15	24.088147934588026	25.103253836818705	24.84366653927557	25.964931689317698
16-17	23.312459072816306	27.485411484419174	26.140988420819728	23.061141021944792
18-19	23.332182343593903	26.7413469038376	27.085526359899966	22.84094439266853
20-21	24.778949450453666	25.107487139258073	26.70491784043461	23.40864556985365
22-23	25.18498486652538	25.787641899023377	25.73009167248222	23.297281561969022
24-25	24.728413155067248	25.45879082044007	25.92550170162575	23.887294322866932
26-27	23.8742023242599	26.941702412261954	26.197121243360034	22.986974020118105
28-29	23.515742032598776	26.577597685420844	27.136909614410023	22.769750667570356
30-31	23.753094007542952	25.301578432742005	27.003994971364715	23.941332588350328
32-33	23.66440193098516	26.0087944752369	27.15246736992669	23.174336223851242
34-35	24.528427342413796	25.51127198199925	26.191161326767627	23.76913934881933
36-37	23.42470299923005	25.636214705491234	26.853272512105203	24.085809783173513
38-39	23.646916576710673	25.930513743711465	26.118527358914363	24.30404232066351
40-41	23.821129489698084	25.86916487313411	26.869967213554414	23.43973842361339
42-43	23.860911270983213	26.001416946591842	26.781296304987695	23.35637547743725
44-45	24.180413517491402	25.492198911278468	26.482966794105124	23.844420777125002
46-47	23.920061241870158	25.300743323758233	26.37409768615107	24.405097748220538
48-49	23.304135603220782	25.33028337461468	27.029178919302183	24.336402102862355
50-51	23.7748105336793	25.5445969412727	26.54824881939742	24.132343705650577
52-53	23.91681128552288	25.931492567168146	25.93752688905005	24.21416925825892
54-55	24.218332696377846	25.334289884029403	26.208650972977445	24.238726446615306
56-57	24.41226119837082	25.494394598161723	25.863412927822942	24.22993127564451
58-59	24.419794697230117	25.611366831277792	26.182167780376904	23.78667069111519
60-61	23.981498093304292	26.106165755935123	26.09744687771941	23.814889273041175
62-63	23.57037017170979	25.579573619762158	26.692010790249444	24.15804541827861
64-65	24.251800796082613	25.193489197458437	26.097183762775426	24.457526243683525
66-67	23.62708155935643	25.76222339928616	25.90917014757253	24.701524893784878
68-69	24.1003841847305	25.56672276344814	26.680668512720114	23.652224539101244
70-71	24.688394671029254	25.33423401074108	26.281174501219155	23.696196817010513
72-73	23.787270276975065	25.557224304433433	26.635690515621054	24.01981490297045
74-75	24.021435228331782	25.078781336533755	26.50410892162319	24.395674513511278
76-77	23.750654418448985	24.830577558852962	26.38285532616998	25.03591269652808
78-79	24.02766159913591	24.379186971154358	26.43536733032377	25.15778409938596
80-81	23.241243164200554	25.859563190529595	26.096815091590074	24.80237855367978
82-83	23.04575839727157	25.82257390425915	26.625759604145387	24.505908094323896
84-85	23.613552526152166	24.874533306364466	26.91731323035249	24.594600937130878
86-87	23.112376827335897	26.110537478684343	26.56540191891221	24.21168377506755
88-89	23.84129974678226	24.883224827407414	25.819940506122595	25.455534919687732
90-91	23.686754231284123	25.255173307765716	25.768760574019815	25.289311886930342
92-93	23.71776390630273	25.443410416121903	26.372192087895385	24.466633589679983
94-95	23.648201662342075	24.69448485945633	26.37839402289911	25.278919455302486
96-97	23.91846075795468	25.478722334341285	26.360291077482838	24.24252583022119
98-99	22.280479437512433	25.91364001063827	26.849852829758557	24.956027722090734
100-101	23.21180167285414	25.303260723710697	26.31955316008409	25.165384443351073
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.5
2	3.5
3	5.0
4	3.0
5	3.0
6	4.5
7	11.5
8	23.0
9	27.5
10	28.5
11	30.5
12	33.5
13	38.5
14	43.0
15	46.0
16	54.0
17	62.5
18	64.0
19	66.5
20	77.5
21	98.5
22	103.5
23	135.0
24	185.5
25	220.5
26	315.5
27	430.0
28	537.0
29	736.5
30	1147.0
31	2107.5
32	4106.0
33	7498.0
34	12489.5
35	19848.5
36	28931.0
37	35922.0
38	37496.0
39	35204.5
40	31726.5
41	27689.5
42	24125.5
43	22292.5
44	21781.0
45	22131.5
46	23399.0
47	25013.5
48	27163.0
49	32290.5
50	38596.0
51	38897.0
52	36737.5
53	38829.5
54	43187.5
55	43429.5
56	35984.5
57	30173.5
58	29537.0
59	29351.0
60	24736.5
61	17970.5
62	14276.5
63	9726.5
64	5382.5
65	3275.0
66	2473.0
67	2210.5
68	1634.5
69	950.0
70	525.5
71	327.0
72	266.5
73	222.5
74	156.5
75	107.0
76	70.0
77	40.0
78	22.0
79	13.5
80	9.0
81	3.5
82	2.0
83	1.5
84	0.5
85	0.5
86	2.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4723527794726009
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.38099324827184E-4
22-23	0.001285085631401682
24-25	2.793664416090613E-4
26-27	0.0018438185146198048
28-29	0.003631763740917797
30-31	7.822260365053717E-4
32-33	2.2349315328724906E-4
34-35	0.0034082705876305477
36-37	0.002123184956228866
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.007934006941697342
46-47	0.08073690162501872
48-49	7.263527481835594E-4
50-51	0.0
52-53	1.1174657664362453E-4
54-55	0.0
56-57	0.008436866536593651
58-59	0.03905542853694677
60-61	0.030339195558744057
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	3.9111301825268583E-4
78-79	0.007542893923444655
80-81	0.005866695273790288
82-83	8.939726131489962E-4
84-85	0.002570171262803364
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06727143913946197
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	894882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.234924205609516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.21914866150172	25.401348297822246
2	15.906664158732852	11.845668686290065
3	6.24406202310051	6.97491528495817
4	2.9952023672260544	4.461045324964974
5	1.7021491030621863	3.1689696419583364
6	1.0405908132514075	2.3247792036281836
7	0.7313039493282912	1.9061032987151278
8	0.5118908097054227	1.5248172400743598
9	0.35822530351596493	1.2004642822453577
>10	1.895283507242303	12.835788781028759
>50	0.17831221125884886	4.6870853727436685
>100	0.18975765326898913	14.999396709161775
>500	0.020782981072216173	5.108885968332781
>1k	0.006626457733170376	3.5607319080762507
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2508	0.2802604142222103	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	2153	0.2405903795137236	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1971	0.22025250256458395	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1672	0.1868402761481402	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1670	0.18661678299485296	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1504	0.1680668512720113	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1501	0.1677316115420804	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1486	0.16605541289242604	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1486	0.16605541289242604	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1457	0.16281476216976093	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1400	0.15644520730107433	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	1337	0.149405172972526	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1321	0.14761722774622799	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	1297	0.144935309906781	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	1288	0.14392959071698838	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1207	0.1348781180088548	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	1183	0.1321962001694078	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	1128	0.12605013845400848	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1084	0.12113328908168897	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1046	0.11688691916923125	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	1042	0.11643993286265676	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	1008	0.11264054925677351	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC	1000	0.11174657664362452	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	993	0.11096435060711915	No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC	980	0.10951164511075204	No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT	968	0.10817068619102853	No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC	927	0.10358907654863994	No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC	924	0.10325383681870906	No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG	904	0.10101890528583658	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	5.587328832181226E-5	0.0
16-17	0.0	0.0	0.0	1.1174657664362452E-4	0.0
18-19	0.0	0.0	0.0	2.2349315328724903E-4	0.0
20-21	0.0	0.0	0.0	5.028595948963103E-4	0.0
22-23	0.0	0.0	0.0	9.498459014708083E-4	0.0
24-25	0.0	0.0	0.0	0.0017879452262979923	0.0
26-27	0.0	0.0	0.0	0.0025142979744815514	0.0
28-29	0.0	0.0	0.0	0.004134623335814107	0.0
30-31	0.0	0.0	0.0	0.009554332303029897	0.0
32-33	0.0	0.0	0.0	0.021399469427254098	0.0
34-35	0.0	0.0	0.0	0.037826216193866904	0.0
36-37	0.0	0.0	0.0	0.06570698706645123	0.0
38-39	0.0	0.0	0.0	0.11588119997943863	0.0
40-41	0.0	0.0	0.0	0.17013416293991834	0.0
42-43	0.0	0.0	0.0	0.2278512697763504	0.0
44-45	0.0	0.0	0.0	0.28903252048873485	0.0
46-47	0.0	0.0	0.0	0.3588182576026784	0.0
48-49	0.0	0.0	0.0	0.4373761009831464	0.0
50-51	0.0	0.0	0.0	0.528561307524344	0.0
52-53	0.0	0.0	0.0	0.6151090311348312	0.0
54-55	0.0	0.0	0.0	0.707746943172396	0.0
56-57	0.0	0.0	0.0	0.8101626806662778	0.0
58-59	0.0	0.0	0.0	0.9334750279925175	0.0
60-61	0.0	0.0	0.0	1.071035063840819	0.0
62-63	0.0	0.0	0.0	1.2011639523423199	0.0
64-65	0.0	0.0	0.0	1.3330249127817968	0.0
66-67	0.0	0.0	0.0	1.4936047434186854	0.0
68-69	0.0	0.0	0.0	1.6782659613222748	0.0
70-71	0.0	0.0	0.0	1.8830415630217168	0.0
72-73	0.0	0.0	0.0	2.096086411392787	0.0
74-75	0.0	0.0	0.0	2.313265883099671	0.0
76-77	0.0	0.0	0.0	2.5347475980073346	0.0
78-79	0.0	0.0	0.0	2.796513953795025	0.0
80-81	0.0	0.0	0.0	3.10124686830219	0.0
82-83	0.0	0.0	0.0	3.427882111831504	0.0
84-85	0.0	0.0	0.0	3.7536233827476693	0.0
86-87	0.0	0.0	0.0	4.094394568222403	0.0
88-89	0.0	0.0	0.0	4.470030685609946	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1140	0.0	57.738678	1
GTATCAA	2115	0.0	46.45681	1
AAACCGT	175	0.0	46.135044	6
CCGTGCG	180	0.0	42.21507	9
AACCGTG	230	0.0	37.167618	7
ATCAACG	2760	0.0	35.618965	3
TCAACGC	2940	0.0	33.43821	4
ACCGTGC	275	0.0	32.812622	8
CGCAGAG	3445	0.0	28.398672	8
CAACGCA	3510	0.0	27.87277	5
TTAATAC	615	0.0	27.800167	3
TATCAAC	3605	0.0	27.796955	2
ACGCAGA	3570	0.0	27.537352	7
TAATATA	190	3.965397E-10	27.49534	4
AACGCAG	3695	0.0	26.477245	6
GTACATG	5465	0.0	26.183254	1
GCAGAGT	3885	0.0	25.426838	9
AATGCGG	225	1.0186341E-10	25.32904	7
ACATGGG	5600	0.0	24.763662	3
TATAGTA	270	1.8189894E-12	24.625456	2
>>END_MODULE