FastQCFastQC Report
Fri 10 Feb 2017
SRR3224530_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224530_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738765
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG23890.323377528713461No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA23350.3160680324595778No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA21780.2948163489066212No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC19040.257727423470251No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA16850.2280833553295026No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT12830.17366821655059458No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT12120.16405758258715558No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA12110.16392222154541702No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC11910.16121500071064546No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG11780.15945530716804396No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA11360.15377014341502374No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA11160.15106292258025217No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG10680.14456559257680046No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC10660.14429487049332332No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC10520.14239981590898323No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA10260.13888042882378024No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA9220.1248028804829682No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA8960.12128349339776519No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC8270.11194358151780336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7250.062.9363021
AAACCGT2900.060.5678676
AACCGTG3100.058.1916247
CCGTGCG3350.052.4851729
GTATCAA16100.047.8252371
ACCGTGC4050.045.7200788
ATCAACG20600.036.8715483
TCAACGC21400.035.7154
TGCGCGG2700.031.68668212-13
CGCAGAG25100.030.832688
TCTAATG8350.030.700532
GTCTAAT7600.030.6443541
GTGCGCG2900.030.3188210-11
ACGCAGA25500.030.1587567
TATCACG804.5075789E-429.670072
TAGGACA2400.029.670074
TATCAAC26350.029.0057322
GCCCATA9550.028.8664021
CTAATGT8200.028.3674833
CACGCTT5200.028.3006827
Produced by FastQC (version 0.11.5)