##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224530_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	738765
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.072525092553114	34.0	31.0	34.0	30.0	34.0
2	32.238396513099566	34.0	31.0	34.0	31.0	34.0
3	32.37870500091369	34.0	31.0	34.0	31.0	34.0
4	35.69079071152532	37.0	35.0	37.0	35.0	37.0
5	35.698729636623284	37.0	37.0	37.0	35.0	37.0
6	35.7964806129148	37.0	37.0	37.0	35.0	37.0
7	35.75029407186318	37.0	37.0	37.0	35.0	37.0
8	35.788560638362675	37.0	37.0	37.0	35.0	37.0
9	37.49911676920266	39.0	38.0	39.0	35.0	39.0
10-11	37.565078881647075	39.0	38.5	39.0	35.0	39.0
12-13	37.55083754644576	39.0	38.0	39.0	35.0	39.0
14-15	39.00285070353902	41.0	39.0	41.0	36.0	41.0
16-17	38.83215704588062	41.0	39.0	41.0	35.5	41.0
18-19	38.91018997922208	41.0	39.0	41.0	35.0	41.0
20-21	38.970181992920615	41.0	39.0	41.0	36.0	41.0
22-23	38.921319364073824	41.0	39.0	41.0	35.0	41.0
24-25	38.846164206479735	41.0	39.0	41.0	35.0	41.0
26-27	38.654046956745375	41.0	39.0	41.0	35.0	41.0
28-29	38.56394861694855	41.0	39.0	41.0	35.0	41.0
30-31	38.47254404309896	40.0	39.0	41.0	34.0	41.0
32-33	38.25961909402855	40.0	38.0	41.0	34.0	41.0
34-35	38.235359688128156	40.0	38.0	41.0	34.0	41.0
36-37	38.21839624237748	40.0	38.0	41.0	34.0	41.0
38-39	38.07589152166115	40.0	38.0	41.0	33.5	41.0
40-41	37.954160660020435	40.0	38.0	41.0	33.0	41.0
42-43	37.76902533281896	40.0	37.5	41.0	33.0	41.0
44-45	37.5442231291412	40.0	37.0	41.0	32.0	41.0
46-47	37.313927974389685	40.0	36.5	41.0	31.5	41.0
48-49	37.05484964772289	40.0	36.0	41.0	31.0	41.0
50-51	35.72664852828707	38.0	34.5	40.0	29.5	40.5
52-53	36.040899338761314	38.5	35.0	40.0	30.0	40.5
54-55	36.45190825228591	39.0	35.0	41.0	30.0	41.0
56-57	36.25867901159367	38.5	35.0	41.0	30.0	41.0
58-59	36.07413453533938	37.5	35.0	41.0	30.0	41.0
60-61	35.798544868801315	37.0	35.0	40.5	30.0	41.0
62-63	35.49972115625402	36.5	35.0	40.0	29.0	41.0
64-65	35.231469411788595	36.0	35.0	40.0	29.0	41.0
66-67	34.99077108417427	35.5	35.0	39.5	29.0	41.0
68-69	34.692993712479606	35.0	34.0	39.0	28.5	41.0
70-71	34.40971012432912	35.0	34.0	39.0	28.0	41.0
72-73	34.10615148254181	35.0	34.0	37.5	27.5	40.5
74-75	33.85941808288156	35.0	34.0	37.0	27.0	39.5
76-77	33.55977205200571	35.0	34.0	37.0	27.0	39.0
78-79	33.3627608238073	35.0	34.0	36.0	27.0	39.0
80-81	33.10079727653584	35.0	33.5	36.0	26.5	38.0
82-83	32.86528598404094	35.0	33.0	35.5	26.0	37.0
84-85	32.66684331282613	35.0	33.0	35.0	26.0	37.0
86-87	32.469988426630934	35.0	33.0	35.0	25.0	36.0
88-89	32.34028141560577	35.0	33.0	35.0	25.0	36.0
90-91	32.16904292975439	35.0	33.0	35.0	25.0	36.0
92-93	32.03219291655668	35.0	33.0	35.0	24.5	36.0
94-95	31.904264549619974	35.0	33.0	35.0	24.0	35.0
96-97	31.77574127090482	35.0	32.0	35.0	24.0	35.0
98-99	31.730017664615946	35.0	32.0	35.0	24.0	35.0
100-101	30.48448762461676	34.0	29.5	35.0	20.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	95.0
8	393.0
9	298.0
10	409.0
11	901.0
12	1509.0
13	1760.0
14	1860.0
15	1864.0
16	2057.0
17	2258.0
18	2487.0
19	2974.0
20	3546.0
21	4728.0
22	5381.0
23	4975.0
24	4537.0
25	4680.0
26	4860.0
27	5297.0
28	5711.0
29	6594.0
30	8125.0
31	10369.0
32	13483.0
33	19212.0
34	32054.0
35	57302.0
36	122830.0
37	188401.0
38	170776.0
39	47014.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.96666363921035	18.851337887169866	13.426181351863173	23.75581712175661
2	16.228299932996286	19.63736776918235	38.325177830568585	25.80915446725278
3	19.578079632900856	26.351546161499257	28.0752336669983	25.995140538601586
4	12.700655824247223	16.60176104715302	38.37702110955446	32.3205620190453
5	13.656575501005056	39.478995350348214	33.20419889951473	13.660230249131997
6	29.867955303784015	37.20344087768099	18.979513106332867	13.949090712202123
7	27.121479766908287	31.342307770400602	22.262965895785534	19.27324656690558
8	26.729474190033365	33.85447334402685	20.358436038523752	19.057616427416026
9	27.106319330233564	14.860476606227962	19.99810494541566	38.03509911812281
10-11	26.701116051789135	24.76443794711444	26.884733304907517	21.64971269618891
12-13	26.870520395524967	22.453080478907367	27.620420566756682	23.05597855881099
14-15	23.978670219824565	24.343667823067904	24.624372713263952	27.053289243843587
16-17	23.682631364352446	26.896250912652235	25.835068332084855	23.586049390910457
18-19	23.668853764145503	26.418205894060716	26.53498498457839	23.37795535721539
20-21	25.26847611999618	24.236820955660562	26.307646787596305	24.18705613674695
22-23	25.13007985274209	25.620059149677672	25.44661934602155	23.80324165155869
24-25	23.99356924066271	25.416730128107496	26.09243370394815	24.49726692728165
26-27	23.89670598904929	26.372053359322656	26.405081453506867	23.32615919812119
28-29	23.705170114989205	26.574688838805304	26.329279270133267	23.390861776072228
30-31	23.770211095544592	25.441649238932545	26.517634159712493	24.270505505810373
32-33	23.75877308751768	25.599683255162333	26.67708946688054	23.96445419043945
34-35	24.440722015796634	25.154413108363283	26.266336385724827	24.13852849011526
36-37	23.552411840244567	25.213693439017405	27.214867255199415	24.019027465538613
38-39	23.80258782489101	25.5749575114538	26.000959757204438	24.621494906450746
40-41	24.496721745316126	25.199074359222536	26.55105407038958	23.753149825071763
42-43	24.412920884422437	25.557434124498137	26.38174085133917	23.647904139740263
44-45	24.256027421646394	25.199526456758043	26.10667718984982	24.43776893174574
46-47	24.21737629692798	25.0774603561349	25.988440167035527	24.716723179901592
48-49	23.807367701501832	25.507773107821833	26.163394313482634	24.521464877193694
50-51	23.8986687241545	25.948170257118296	25.661475570715993	24.491685448011207
52-53	24.46386875393393	25.83866317435179	24.984061237335283	24.713406834378997
54-55	24.207494940881066	25.03353569809073	25.6264170609057	25.1325523001225
56-57	24.2402523129818	25.811252563399727	25.349874452633784	24.598620670984683
58-59	24.63807357055391	25.751250758984444	25.830584517868033	23.78009115259361
60-61	24.28669292223379	26.059620625557617	25.40178734615244	24.251899106056154
62-63	24.06094291594454	25.55073386048527	25.950092071057192	24.438231152512998
64-65	25.093103943273633	24.85204937014336	25.276806487347343	24.77804019923567
66-67	23.901630022744502	25.431468645077437	25.04738438210766	25.6195169500704
68-69	24.0397149296461	25.736330226797428	26.246302951547516	23.97765189200896
70-71	24.650802352574903	25.083890005617484	25.85761372019519	24.40769392161242
72-73	24.36755937273693	25.49647046083667	26.117033156687175	24.018937009739226
74-75	24.24526067152613	25.22980920861167	26.033989157580557	24.490940962281645
76-77	24.36302477783869	24.808701007762956	25.395220401616207	25.43305381278214
78-79	24.30983282192289	24.275986995079492	26.076991133070283	25.337189049927332
80-81	23.978102362785936	25.722274629255043	25.512074648080947	24.787548359878077
82-83	24.043263315630846	25.79493600087215	25.75911571982175	24.402684963675256
84-85	23.974953693172587	25.02281491350643	26.323129583292715	24.67910181002827
86-87	23.948425194184292	26.38143415341067	25.433164712972932	24.23697593943211
88-89	24.23324741831811	25.36605280422141	25.152923987892006	25.247775789568465
90-91	23.76215711356115	25.185207745358806	25.706347756052327	25.346287385027715
92-93	23.78128720437014	25.36215355730463	26.133060255935607	24.72349898238962
94-95	24.308948041664127	25.083890005617484	26.091517600319452	24.515644352398937
96-97	24.34975939574831	25.787361339532872	26.01476788965368	23.84811137506514
98-99	23.14347942027259	26.14851081743622	25.982151196395044	24.725858565896143
100-101	24.155145773325152	25.226863080462756	25.541580166865057	25.07641097934703
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	2.0
2	2.0
3	3.5
4	5.0
5	6.0
6	7.5
7	15.0
8	26.5
9	36.0
10	46.5
11	52.0
12	49.0
13	60.0
14	74.5
15	85.0
16	102.5
17	105.5
18	117.0
19	148.0
20	168.5
21	197.5
22	258.5
23	349.5
24	473.0
25	650.5
26	941.5
27	1354.0
28	1884.5
29	2747.0
30	3998.0
31	5364.5
32	7089.5
33	8901.5
34	10624.0
35	12149.5
36	13836.5
37	15278.0
38	16203.0
39	17250.0
40	18317.5
41	18660.5
42	18516.5
43	19088.0
44	19975.5
45	22112.0
46	25191.5
47	26173.5
48	27517.0
49	31061.5
50	34909.5
51	35032.0
52	33411.0
53	35203.5
54	37319.0
55	37215.0
56	31720.0
57	26052.5
58	24267.0
59	22955.5
60	19339.0
61	14643.0
62	11940.0
63	8576.5
64	5160.5
65	3430.0
66	2714.5
67	2246.0
68	1737.0
69	1166.0
70	691.0
71	455.5
72	323.5
73	241.5
74	192.0
75	144.5
76	101.0
77	70.5
78	52.0
79	40.0
80	27.0
81	14.5
82	13.0
83	11.5
84	8.5
85	8.5
86	7.0
87	4.0
88	3.0
89	3.5
90	4.5
91	4.5
92	4.5
93	4.5
94	3.5
95	3.5
96	4.0
97	4.5
98	2.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1451070367437548
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.036141398144200115
16-17	0.07201207420492309
18-19	0.025853958972068252
20-21	0.039457743666795254
22-23	0.06341664805452343
24-25	0.017258532821668594
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.020101114698178716
38-39	0.004940678023458068
40-41	0.004534594898242338
42-43	0.003113303959987276
44-45	0.010625841776478313
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.015363478237328514
60-61	0.017326213342537887
62-63	0.027749013556408332
64-65	0.04642883731633199
66-67	0.016920130217322152
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01644636657123713
80-81	0.05691931805107172
82-83	0.04825621137980278
84-85	0.02883190189031695
86-87	0.05685163753020243
88-89	0.03309577470508213
90-91	0.0
92-93	0.0033163455225951417
94-95	0.0
96-97	0.0
98-99	5.41444166954309E-4
100-101	0.04385697752329902
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	738765.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.007189505090686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.21277440278004	38.11186652268546
2	13.856557142736717	13.858549578499058
3	4.440686286907186	6.661987220460755
4	1.9584041537821646	3.9173715058296588
5	1.0198455652097114	2.549980522268418
6	0.5720308446119127	1.7163392909558997
7	0.3633777663525028	1.2720050576748319
8	0.24222904248583865	0.9690554904980796
9	0.18090620058189252	0.8141949589630182
>10	0.9162527201784322	8.510297527375522
>50	0.10872149895858943	3.8309236919786356
>100	0.11476147521092908	11.844235978948513
>500	0.009060116463996473	2.781306523330672
>1k	0.004392783740119502	3.161886130531413
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2389	0.323377528713461	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2335	0.3160680324595778	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2178	0.2948163489066212	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	1904	0.257727423470251	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1685	0.2280833553295026	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1283	0.17366821655059458	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	1212	0.16405758258715558	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1211	0.16392222154541702	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1191	0.16121500071064546	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1178	0.15945530716804396	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1136	0.15377014341502374	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1116	0.15106292258025217	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1068	0.14456559257680046	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1066	0.14429487049332332	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1052	0.14239981590898323	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1026	0.13888042882378024	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	922	0.1248028804829682	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	896	0.12128349339776519	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	827	0.11194358151780336	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	1.3536104173857721E-4	0.0
18-19	0.0	0.0	0.0	1.3536104173857721E-4	0.0
20-21	0.0	0.0	0.0	2.7072208347715443E-4	0.0
22-23	0.0	0.0	0.0	5.414441669543089E-4	0.0
24-25	0.0	0.0	0.0	0.0010152078130393292	0.0
26-27	0.0	0.0	0.0	0.0016243325008629267	0.0
28-29	0.0	0.0	0.0	0.00291026239737941	0.0
30-31	0.0	0.0	0.0	0.005685163753020243	0.0
32-33	0.0	0.0	0.0	0.011099605422563332	0.0
34-35	0.0	0.0	0.0	0.017258532821668594	0.0
36-37	0.0	0.0	0.0	0.027952055119016196	0.0
38-39	0.0	0.0	0.0	0.049948224401535	0.0
40-41	0.0	0.0	0.0	0.07708811327011972	0.0
42-43	0.0	0.0	0.0	0.1009793371369786	0.0
44-45	0.0	0.0	0.0	0.12676561558817756	0.0
46-47	0.0	0.0	0.0	0.15695112789588028	0.0
48-49	0.0	0.0	0.0	0.19573206635398266	0.0
50-51	0.0	0.0	0.0	0.2382354334598959	0.0
52-53	0.0	0.0	0.0	0.28188936942058707	0.0
54-55	0.0	0.0	0.0	0.32452809756823886	0.0
56-57	0.0	0.0	0.0	0.372378225822826	0.0
58-59	0.0	0.0	0.0	0.4296359464782441	0.0
60-61	0.0	0.0	0.0	0.49312027505363687	0.0
62-63	0.0	0.0	0.0	0.563981780403782	0.0
64-65	0.0	0.0	0.0	0.630579412939162	0.0
66-67	0.0	0.0	0.0	0.711254593815354	0.0
68-69	0.0	0.0	0.0	0.8047213931358416	0.0
70-71	0.0	0.0	0.0	0.9028581483963101	0.0
72-73	0.0	0.0	0.0	1.0110116207454332	0.0
74-75	0.0	0.0	0.0	1.1181498852815173	0.0
76-77	0.0	0.0	0.0	1.2282660927358497	0.0
78-79	0.0	0.0	0.0	1.3592955811387926	0.0
80-81	0.0	0.0	0.0	1.5131334050746854	0.0
82-83	0.0	0.0	0.0	1.6809810968305212	0.0
84-85	0.0	0.0	0.0	1.8508592042124357	0.0
86-87	0.0	0.0	0.0	2.0317015559751748	0.0
88-89	0.0	0.0	0.0	2.2332541471239162	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	725	0.0	62.936302	1
AAACCGT	290	0.0	60.567867	6
AACCGTG	310	0.0	58.191624	7
CCGTGCG	335	0.0	52.485172	9
GTATCAA	1610	0.0	47.825237	1
ACCGTGC	405	0.0	45.720078	8
ATCAACG	2060	0.0	36.871548	3
TCAACGC	2140	0.0	35.715	4
TGCGCGG	270	0.0	31.686682	12-13
CGCAGAG	2510	0.0	30.83268	8
TCTAATG	835	0.0	30.70053	2
GTCTAAT	760	0.0	30.644354	1
GTGCGCG	290	0.0	30.31882	10-11
ACGCAGA	2550	0.0	30.158756	7
TATCACG	80	4.5075789E-4	29.67007	2
TAGGACA	240	0.0	29.67007	4
TATCAAC	2635	0.0	29.005732	2
GCCCATA	955	0.0	28.866402	1
CTAATGT	820	0.0	28.367483	3
CACGCTT	520	0.0	28.300682	7
>>END_MODULE