##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224530_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	738765
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.161013312758456	34.0	33.0	34.0	31.0	34.0
2	33.282466007458396	34.0	34.0	34.0	31.0	34.0
3	33.39942606918303	34.0	34.0	34.0	31.0	34.0
4	36.62157113561146	37.0	37.0	37.0	35.0	37.0
5	36.65526520612103	37.0	37.0	37.0	35.0	37.0
6	36.69170169133621	37.0	37.0	37.0	36.0	37.0
7	36.6675180876192	37.0	37.0	37.0	35.0	37.0
8	36.65147645056276	37.0	37.0	37.0	35.0	37.0
9	38.48991221836443	39.0	39.0	39.0	37.0	39.0
10-11	38.512583162440016	39.0	39.0	39.0	37.5	39.0
12-13	38.494170676737525	39.0	39.0	39.0	37.0	39.0
14-15	40.08051748526256	41.0	40.0	41.0	38.0	41.0
16-17	40.08882526919927	41.0	40.0	41.0	38.0	41.0
18-19	40.08448627100634	41.0	40.0	41.0	38.0	41.0
20-21	40.08237666917084	41.0	40.0	41.0	38.0	41.0
22-23	40.000622660792	41.0	40.0	41.0	38.0	41.0
24-25	39.95510006565011	41.0	40.0	41.0	38.0	41.0
26-27	39.87524652629727	41.0	40.0	41.0	38.0	41.0
28-29	39.847753345109744	41.0	40.0	41.0	38.0	41.0
30-31	39.77601673062476	41.0	40.0	41.0	37.0	41.0
32-33	39.709706740303076	41.0	40.0	41.0	37.0	41.0
34-35	39.62007674971066	41.0	40.0	41.0	37.0	41.0
36-37	39.48550689326105	41.0	40.0	41.0	36.0	41.0
38-39	39.349970558973425	41.0	39.0	41.0	36.0	41.0
40-41	39.19683390523373	41.0	39.0	41.0	35.0	41.0
42-43	39.07003377257991	41.0	39.0	41.0	35.0	41.0
44-45	38.77761128369644	40.5	38.0	41.0	35.0	41.0
46-47	38.515523881071786	40.0	38.0	41.0	35.0	41.0
48-49	38.37366347891414	40.0	37.5	41.0	35.0	41.0
50-51	38.16757967689319	40.0	37.0	41.0	35.0	41.0
52-53	37.881284305564016	40.0	36.0	41.0	34.0	41.0
54-55	37.6450874093927	39.0	35.5	41.0	34.0	41.0
56-57	37.3784410468823	39.0	35.0	41.0	34.0	41.0
58-59	37.07587527833614	38.5	35.0	41.0	33.0	41.0
60-61	36.803415159083066	37.5	35.0	41.0	33.0	41.0
62-63	36.57408715897478	37.0	35.0	40.5	33.0	41.0
64-65	36.29251250397623	36.5	35.0	40.0	33.0	41.0
66-67	36.01531542506751	36.0	35.0	40.0	33.0	41.0
68-69	35.747541505079425	35.5	35.0	39.0	32.0	41.0
70-71	35.467241274288845	35.0	35.0	39.0	32.0	41.0
72-73	35.18687742380865	35.0	35.0	38.5	32.0	40.5
74-75	34.89962437310918	35.0	35.0	37.0	31.0	40.0
76-77	33.130513086028714	34.5	32.0	35.5	28.5	38.5
78-79	34.44587791787646	35.0	34.5	36.0	31.5	39.0
80-81	34.408657692229596	35.0	35.0	36.0	32.0	38.5
82-83	34.23495563541857	35.0	35.0	36.0	32.0	37.0
84-85	34.05362530710036	35.0	35.0	35.5	32.0	37.0
86-87	33.89250099828768	35.0	35.0	35.0	31.5	36.5
88-89	33.76535637178264	35.0	34.5	35.0	31.0	36.0
90-91	33.62736729541871	35.0	34.0	35.0	31.0	36.0
92-93	33.53694882675817	35.0	34.0	35.0	31.0	36.0
94-95	33.44869072032378	35.0	34.0	35.0	31.0	36.0
96-97	33.41383051443964	35.0	34.0	35.0	31.0	35.0
98-99	33.386113310728035	35.0	34.0	35.0	31.0	35.0
100-101	32.533556002246996	34.5	33.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	3.0
14	11.0
15	43.0
16	130.0
17	286.0
18	565.0
19	860.0
20	1230.0
21	1846.0
22	2599.0
23	2583.0
24	2105.0
25	2303.0
26	2997.0
27	3920.0
28	4974.0
29	5745.0
30	6120.0
31	6679.0
32	7975.0
33	10856.0
34	19034.0
35	39603.0
36	112739.0
37	223669.0
38	213430.0
39	66411.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.489160297037785	18.7562222887142	13.334058700323695	23.420558713924326
2	16.147489391078356	19.631276522304116	38.9915602390476	25.229673847569934
3	19.31967540422191	26.784430772979228	28.27989956210703	25.61599426069183
4	12.761297570946105	16.69989780241349	38.781479902269325	31.757324724371077
5	13.708283418949192	38.48720499752966	34.178256955865535	13.626254627655616
6	29.936312629861998	36.60081351986085	19.461804498047417	14.001069352229734
7	27.338734238898706	30.940420837478765	22.481709339235074	19.239135584387455
8	26.912346957422184	33.50036885883873	20.13766217944813	19.449622004290944
9	27.19904164382449	14.961997387531895	20.214276529072166	37.624684439571446
10-11	26.97853850683235	24.514493783544157	26.988555223921	21.51841248570249
12-13	27.18767131631845	22.626816375978827	27.598762800078507	22.58674950762421
14-15	24.226513167245333	24.61736817526548	24.847617307262794	26.308501350226393
16-17	23.545241044175075	27.029163536442574	26.003262201105898	23.42233321827645
18-19	23.517762752702147	26.278248157397822	27.133932982748238	23.0700561071518
20-21	25.092469200057664	24.600834236311005	26.707203088420524	23.59949347521081
22-23	25.117020729386347	25.489876251763093	25.88479583300621	23.508307185844348
24-25	24.469993123649772	25.032689735829035	26.298858634151095	24.1984585063701
26-27	23.999542470079508	26.388105846433735	26.423435819584313	23.188915863902444
28-29	23.794172609817725	26.29946712998089	26.84113596815098	23.065224292050402
30-31	23.877040237586666	25.160471166627413	26.933708014218382	24.028780581567542
32-33	23.853032804138266	25.497469758589858	27.259806419781164	23.389691017490712
34-35	24.62825165586222	25.235502660614912	26.3057724040561	23.830473279466766
36-37	23.630512248689513	25.264279985516126	27.07036524411085	24.034842521683515
38-39	23.919852727186587	25.49714726604536	26.314118833458544	24.26888117330951
40-41	24.170676737528172	25.579717501505893	26.678104674693575	23.57150108627236
42-43	24.235785398604428	25.56550459212334	26.831401054462518	23.367308954809715
44-45	24.38424128793391	25.20574926983461	26.419972857814884	23.990036584416597
46-47	24.131243793630862	24.983438392853515	26.20154629189341	24.683771521622212
48-49	23.77950890681683	25.163720288050246	26.652363419784503	24.404407385348424
50-51	24.28133438914946	25.367809790664147	26.027424147056237	24.323431673130155
52-53	24.572444940166996	25.736347645291563	25.421429968548843	24.2697774459926
54-55	24.32045373021191	24.900137391457363	26.1048506629307	24.674558215400026
56-57	24.610281093074306	25.443563097878652	25.457709466086282	24.48844634296076
58-59	24.926775763895	25.35880891284183	25.989830531425824	23.72458479183734
60-61	24.377232390685634	25.796619899858914	25.64625933237471	24.179888377080744
62-63	24.215210520260165	25.375457689522378	26.153851360040065	24.25548043017739
64-65	24.554019207731823	25.129303635120774	25.737616156693942	24.579061000453457
66-67	24.012913443381862	25.214716452457818	25.723200205748782	25.049169898411538
68-69	24.514899866669374	25.372750468687606	26.140585977949687	23.971763686693333
70-71	24.990829289422212	25.070421581964496	25.979235616197304	23.95951351241599
72-73	24.31632521843888	25.28794677603839	26.186134968494716	24.209593037028014
74-75	24.429351688290595	25.010998084641262	26.149452126183565	24.410198100884585
76-77	24.44175376591593	24.42875909711417	25.898577963613572	25.230909173356324
78-79	24.290585725347498	24.383585936527016	25.96472489806527	25.361103440060212
80-81	23.79354059682844	25.48883442542284	25.947735377946902	24.76988959980182
82-83	23.919505549170974	25.44300644729878	26.236770042733827	24.400717960796417
84-85	24.031003554633884	24.86125286631274	26.54076999921489	24.566973579838486
86-87	23.765202737000262	25.96509038733562	25.939777872530506	24.329929003133607
88-89	24.311452220936292	24.96714110711796	25.295459313854877	25.425947358090866
90-91	24.228746624434024	25.122264860950374	25.568617896083328	25.08037061853228
92-93	24.206885816193243	25.305543711464402	26.11635635147848	24.371214120863876
94-95	24.228543582871414	25.071166067694055	25.869119408742968	24.83117094069156
96-97	24.336764735741408	25.635486250702183	26.02431084309625	24.00343817046016
98-99	22.691789676013347	26.151888624934855	26.349041982227096	24.807279716824702
100-101	23.81106214558406	25.313484347624087	25.89158910405276	24.98386440273909
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	2.5
3	4.5
4	4.0
5	4.0
6	9.0
7	19.5
8	33.5
9	45.0
10	46.5
11	47.5
12	58.0
13	67.0
14	78.5
15	99.0
16	111.0
17	109.0
18	122.5
19	149.0
20	170.0
21	210.0
22	257.5
23	336.0
24	467.0
25	636.5
26	956.0
27	1407.0
28	1943.5
29	2735.0
30	3958.0
31	5338.0
32	7125.5
33	9094.0
34	10626.0
35	12073.0
36	13940.5
37	15454.5
38	16311.0
39	17260.0
40	18428.0
41	18845.0
42	18667.0
43	19337.5
44	20702.5
45	22726.0
46	24834.5
47	26478.0
48	27982.5
49	31410.0
50	36020.5
51	35948.5
52	33085.0
53	34293.0
54	37108.0
55	36583.0
56	31004.5
57	25772.0
58	24536.5
59	23995.5
60	19940.5
61	14428.5
62	11571.5
63	7978.5
64	4494.5
65	2968.5
66	2294.0
67	1854.0
68	1448.5
69	961.5
70	518.5
71	318.0
72	240.0
73	207.5
74	155.0
75	120.0
76	85.5
77	41.5
78	28.0
79	17.0
80	10.5
81	5.0
82	3.5
83	4.0
84	1.5
85	0.5
86	0.5
87	0.0
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4744404512937132
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.444857295621747E-4
22-23	9.475272921700405E-4
24-25	1.3536104173857724E-4
26-27	0.0020980961469479467
28-29	0.0039931507312880275
30-31	4.0608312521573167E-4
32-33	2.0304156260786584E-4
34-35	0.0035193870852030075
36-37	0.0016920130217322152
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.008460065108661076
46-47	0.08284095754400926
48-49	6.768052086928862E-4
50-51	0.0
52-53	6.768052086928862E-5
54-55	0.0
56-57	0.008053981983445344
58-59	0.03790109168680162
60-61	0.028019735639885485
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	6.768052086928862E-5
78-79	0.007715579379098901
80-81	0.005888205315628109
82-83	8.121662504314633E-4
84-85	0.0014889714591243494
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06639459097277213
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	738765.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.764786722777544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.484522706082	35.57737341203888
2	14.730256466722436	14.071751170096238
3	4.832070457411344	6.924084444830611
4	2.105486442807002	4.022724435535041
5	1.0901704896130415	2.6035880463916454
6	0.6321899102548986	1.8117849738970264
7	0.3923343609695232	1.3117836953008548
8	0.2739354502516859	1.0467574685663852
9	0.19858063224906347	0.8536645391985755
>10	1.001981640969121	8.885033742485572
>50	0.11841867931817467	4.009269444591426
>100	0.12468111859372093	12.50219176124418
>500	0.01053242326018652	3.349554040653087
>1k	0.004839221497923537	3.03043882517053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	1837	0.24865823367376635	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1796	0.24310843096248472	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1757	0.23782935033468017	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1682	0.22767727220428688	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	1421	0.19234804031051822	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1330	0.1800301855123077	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1272	0.17217924509147023	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1237	0.16744160863062002	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1228	0.1662233592549728	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1178	0.15945530716804396	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1131	0.15309333820633084	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	1124	0.1521458109141608	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1081	0.14632528611940196	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	1073	0.14524239778549336	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1072	0.1451070367437548	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1052	0.14239981590898323	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1017	0.13766217944813303	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	964	0.13048804423598845	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	959	0.12981123902729555	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	959	0.12981123902729555	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	948	0.1283222675681712	No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT	927	0.1254796856916611	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	862	0.11668121797865356	No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA	766	0.10368655797175015	No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT	753	0.10192686442914865	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.3536104173857721E-4	0.0
10-11	0.0	0.0	0.0	1.3536104173857721E-4	0.0
12-13	0.0	0.0	0.0	1.3536104173857721E-4	0.0
14-15	0.0	0.0	0.0	1.3536104173857721E-4	0.0
16-17	0.0	0.0	0.0	2.7072208347715443E-4	0.0
18-19	0.0	0.0	0.0	2.7072208347715443E-4	0.0
20-21	0.0	0.0	0.0	4.0608312521573167E-4	0.0
22-23	0.0	0.0	0.0	6.76805208692886E-4	0.0
24-25	0.0	0.0	0.0	0.0010152078130393292	0.0
26-27	0.0	0.0	0.0	0.0016243325008629267	0.0
28-29	0.0	0.0	0.0	0.00291026239737941	0.0
30-31	0.0	0.0	0.0	0.005752844273889532	0.0
32-33	0.0	0.0	0.0	0.011302646985171198	0.0
34-35	0.0	0.0	0.0	0.01766461594688433	0.0
36-37	0.0	0.0	0.0	0.028425818765101217	0.0
38-39	0.0	0.0	0.0	0.0504896685684893	0.0
40-41	0.0	0.0	0.0	0.07776491847881262	0.0
42-43	0.0	0.0	0.0	0.10172382286654078	0.0
44-45	0.0	0.0	0.0	0.12825458704730192	0.0
46-47	0.0	0.0	0.0	0.15918458508456682	0.0
48-49	0.0	0.0	0.0	0.19857464823049278	0.0
50-51	0.0	0.0	0.0	0.24202554262857606	0.0
52-53	0.0	0.0	0.0	0.28608556171448296	0.0
54-55	0.0	0.0	0.0	0.3286566093412655	0.0
56-57	0.0	0.0	0.0	0.3771835428045454	0.0
58-59	0.0	0.0	0.0	0.4357948738773494	0.0
60-61	0.0	0.0	0.0	0.49968528557795777	0.0
62-63	0.0	0.0	0.0	0.5717650403037502	0.0
64-65	0.0	0.0	0.0	0.6387010754434765	0.0
66-67	0.0	0.0	0.0	0.7205945056953158	0.0
68-69	0.0	0.0	0.0	0.8146027491827577	0.0
70-71	0.0	0.0	0.0	0.9146345590275662	0.0
72-73	0.0	0.0	0.0	1.0252245301279839	0.0
74-75	0.0	0.0	0.0	1.134257849248408	0.0
76-77	0.0	0.0	0.0	1.246404472328819	0.0
78-79	0.0	0.0	0.0	1.3803442231291412	0.0
80-81	0.0	0.0	0.0	1.5358740600867664	0.0
82-83	0.0	0.0	0.0	1.708120985699106	0.0
84-85	0.0	0.0	0.0	1.8815184801662235	0.0
86-87	0.0	0.0	0.0	2.06642166318112	0.0
88-89	0.0	0.0	0.0	2.271425960894195	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	860	0.0	56.490067	1
CCGTGCG	310	0.0	50.55072	9
AAACCGT	320	0.0	48.971012	6
GTATCAA	1650	0.0	47.917538	1
AACCGTG	355	0.0	45.480545	7
ACCGTGC	360	0.0	44.84887	8
ATCAACG	2380	0.0	33.12121	3
TCAACGC	2495	0.0	31.594584	4
TATCAAC	2770	0.0	28.972239	2
ACGCAGA	2795	0.0	28.373293	7
CGCAGAG	2795	0.0	28.373293	8
CAACGCA	2835	0.0	27.80546	5
AACGCAG	2885	0.0	27.488165	6
GTACAAC	160	6.0737875E-8	26.791245	1
CAAAACC	700	0.0	25.778679	4
TACATGG	4015	0.0	25.31065	2
GTCTAAT	660	0.0	25.257738	1
GCAGAGT	3220	0.0	24.628372	9
GTATTGG	215	1.6079866E-9	24.368263	1
GTGCGCG	335	0.0	24.0979	10-11
>>END_MODULE