FastQCFastQC Report
Fri 10 Feb 2017
SRR3224528_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224528_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043550
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC35670.34181400028748027No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA27700.26544008432753585No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA25240.2418667049973648No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA23860.22864261415361026No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG19320.1851372718125629No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT19180.1837956973791385No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC16440.15753916918211874No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA16070.15399357960806861No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG14680.14067366201906953No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA13860.1328158689090125No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC13690.13118681423985434No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC13400.128407838627761No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA13300.12744957117531505No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA12610.12083752575343777No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG12310.11796272339609985No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA10850.10397201859038858No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT10550.10109721623305064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGT4250.062.5595446
GGTATCA9100.060.1036261
AACCGTG4800.057.3695227
ACCGTGC5200.054.785198
CCGTGCG5150.053.5167779
GTATCAA20000.049.700471
TATCACG756.9434864E-637.982582
ATCAACG28600.034.6956253
GCGTAAT550.002563527634.589281
GTCTAAT10800.033.468331
TCAACGC30250.032.6461834
CTAATGT11600.032.334313
TAGACCG1452.4103429E-829.4692425
CGCAGAG34200.028.8770288
TGCGCGG4750.028.51699412-13
CCCATAA11750.028.28492
ACGCAGA35000.028.215637
TATCAAC35850.028.208822
TCTAATG13050.028.013972
AATGCGG3300.027.3359457
Produced by FastQC (version 0.11.5)