##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224528_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1043550
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.245526328398256	34.0	31.0	34.0	31.0	34.0
2	32.409852905946046	34.0	31.0	34.0	31.0	34.0
3	32.544527813712804	34.0	31.0	34.0	31.0	34.0
4	35.840677495088876	37.0	37.0	37.0	35.0	37.0
5	35.859474869436056	37.0	37.0	37.0	35.0	37.0
6	35.949138038426526	37.0	37.0	37.0	35.0	37.0
7	35.904077428010154	37.0	37.0	37.0	35.0	37.0
8	35.94565281970198	37.0	37.0	37.0	35.0	37.0
9	37.67190072349192	39.0	39.0	39.0	35.0	39.0
10-11	37.73775286282402	39.0	39.0	39.0	35.0	39.0
12-13	37.73328110775718	39.0	39.0	39.0	35.0	39.0
14-15	39.220895980068036	41.0	40.0	41.0	36.0	41.0
16-17	39.054501461357866	41.0	40.0	41.0	36.0	41.0
18-19	39.14619759474869	41.0	40.0	41.0	36.0	41.0
20-21	39.20859230511236	41.0	40.0	41.0	36.0	41.0
22-23	39.160717263188154	41.0	40.0	41.0	36.0	41.0
24-25	39.0942609362273	41.0	40.0	41.0	36.0	41.0
26-27	38.922578697714535	41.0	39.0	41.0	35.0	41.0
28-29	38.8434770734512	41.0	39.0	41.0	35.0	41.0
30-31	38.748505581907914	41.0	39.0	41.0	35.0	41.0
32-33	38.543964831584496	41.0	39.0	41.0	35.0	41.0
34-35	38.529348857266065	40.5	39.0	41.0	35.0	41.0
36-37	38.50328829476307	40.0	38.5	41.0	35.0	41.0
38-39	38.37965981505438	40.0	38.0	41.0	34.5	41.0
40-41	38.269056585693065	40.0	38.0	41.0	34.0	41.0
42-43	38.101837956973796	40.0	38.0	41.0	33.5	41.0
44-45	37.89769105457333	40.0	38.0	41.0	33.0	41.0
46-47	37.687239231469505	40.0	37.0	41.0	33.0	41.0
48-49	37.444983469886445	40.0	37.0	41.0	32.5	41.0
50-51	36.14025346174117	38.5	35.0	40.0	30.0	40.5
52-53	36.452127832878155	39.0	35.0	40.0	30.5	40.5
54-55	36.8652896363375	39.0	35.0	41.0	31.0	41.0
56-57	36.684070241004264	39.0	35.0	41.0	31.0	41.0
58-59	36.49979972210244	39.0	35.0	41.0	31.0	41.0
60-61	36.231108236308756	38.0	35.0	41.0	31.0	41.0
62-63	35.938658904700304	37.0	35.0	40.5	30.5	41.0
64-65	35.677636433328544	37.0	35.0	40.0	30.0	41.0
66-67	35.44608116525322	36.0	35.0	40.0	30.0	41.0
68-69	35.14768195103254	36.0	35.0	39.5	29.5	41.0
70-71	34.867231086196156	35.0	35.0	39.0	29.0	41.0
72-73	34.56247328829477	35.0	34.0	39.0	29.0	41.0
74-75	34.29409755162666	35.0	34.0	37.5	29.0	40.0
76-77	33.986117579416415	35.0	34.0	37.0	28.5	39.5
78-79	33.777347994825355	35.0	34.0	36.5	29.0	39.0
80-81	33.50769919984668	35.0	34.0	36.0	28.0	39.0
82-83	33.2609007714053	35.0	34.0	36.0	27.0	37.0
84-85	33.046999185472664	35.0	34.0	35.5	27.0	37.0
86-87	32.83275262325715	35.0	34.0	35.0	27.0	37.0
88-89	32.70133678309617	35.0	33.0	35.0	27.0	36.0
90-91	32.534509127497486	35.0	33.0	35.0	26.0	36.0
92-93	32.402462268219054	35.0	33.0	35.0	25.5	36.0
94-95	32.26610847587561	35.0	33.0	35.0	25.0	35.5
96-97	32.14508169230032	35.0	33.0	35.0	25.0	35.0
98-99	32.09335537348474	35.0	33.0	35.0	25.0	35.0
100-101	30.89480283647166	34.0	31.0	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	4.0
7	156.0
8	475.0
9	407.0
10	458.0
11	969.0
12	1662.0
13	1963.0
14	2114.0
15	2116.0
16	2376.0
17	2802.0
18	3231.0
19	3863.0
20	4592.0
21	5873.0
22	6936.0
23	6392.0
24	5821.0
25	5923.0
26	6119.0
27	6709.0
28	7279.0
29	8248.0
30	10073.0
31	12769.0
32	16871.0
33	23785.0
34	39014.0
35	71123.0
36	155971.0
37	269458.0
38	270692.0
39	87276.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.30965224509538	20.134411104510725	13.710969101568384	23.84496754882551
2	15.74136361458483	20.272147956494656	38.9890278376695	24.99746059125102
3	17.867584559996704	27.990252494133	29.24270925466844	24.89945369120185
4	12.195103253318	17.512241866704997	40.1392362608404	30.153418619136602
5	12.970245795601553	39.88711609410186	34.33108140482008	12.811556705476498
6	28.18341239039816	39.19150975037133	19.602031526999188	13.023046332231324
7	25.32739207512817	32.22567198505103	24.252120166738536	18.19481577308227
8	25.894878060466674	34.31680321977864	21.282257678117965	18.50606104163672
9	26.630444156964213	14.973887211920847	21.364477025537827	37.03119160557712
10-11	25.76982415792248	25.446935939820804	27.94667241627138	20.836567485985338
12-13	25.91806813281587	23.1933783719036	28.63672080877773	22.251832686502805
14-15	23.49607680546036	25.020970038009693	25.385728870589702	26.097224285940246
16-17	23.00820839808453	27.339877739509106	26.584776080328087	23.067137782078277
18-19	22.965753664286513	26.860539289806805	27.47710464932935	22.69660239657733
20-21	24.711849198124465	25.12402731428977	26.735735301025677	23.42838818656009
22-23	24.486079619961423	26.318141347462674	25.987650088723935	23.208128943851968
24-25	23.440076560306817	26.116716130234945	26.630680383242417	23.812526926215817
26-27	23.411767524316037	26.752192036797467	26.983949020171533	22.852091418714963
28-29	23.318001054094196	26.790187341286952	26.842508744190503	23.049302860428345
30-31	23.39058981361698	25.875473144554643	27.140050788174978	23.593886253653395
32-33	23.497772028173063	25.989938191749317	27.281778544391738	23.23051123568588
34-35	23.85386421350199	25.741555268075324	26.887164007474485	23.517416510948205
36-37	23.387009426863926	25.731264886729065	27.50233634782109	23.379389338585923
38-39	23.352400794259253	26.14074143641326	26.568729108528323	23.938128660799165
40-41	23.950594331390963	25.732318718983098	27.08150152829441	23.235585421331532
42-43	23.872758225967278	26.25831389172787	26.858500090320213	23.010427791984643
44-45	23.726627951051373	25.765259547804554	26.522480629813195	23.985631871330877
46-47	23.879402041109675	25.642230846629293	26.288821810167217	24.189545302093816
48-49	23.461932825451584	25.979061856164055	26.583009918068136	23.975995400316226
50-51	23.479660773321832	26.506588088735565	26.011595036174594	24.002156101768005
52-53	24.193665852139333	26.19788222893009	25.40448469167745	24.203967227253127
54-55	23.690958746586173	25.714388385798475	26.095587178381486	24.499065689233866
56-57	23.7436748328661	26.38758391432318	25.781527373641595	24.087213879169127
58-59	24.329995912286194	26.140666235052652	26.134100973719303	23.39523687894185
60-61	23.98055712100475	26.367355975479317	25.905628332969126	23.746458570546807
62-63	23.737412225268866	26.147056553982388	26.421206557169626	23.694324663579124
64-65	24.567018893503114	25.56885920402136	25.634772904531555	24.229348997943973
66-67	23.58287575848482	25.944565793496604	25.61970282634136	24.85285562167721
68-69	23.589286569881654	26.229505054860812	26.59402999377126	23.587178381486275
70-71	24.201188251641035	25.5857409803076	26.198696756264674	24.01437401178669
72-73	24.1104403238944	25.812227492693214	26.340520339226682	23.736811844185713
74-75	23.91802021944325	25.61798668008241	26.39403957644579	24.069953524028556
76-77	23.89736955584304	25.21388529538594	25.840208902304635	25.04853624646639
78-79	23.837921170759945	25.04378896379138	26.45385350069703	24.664436364751648
80-81	23.600808879649907	26.097059804933053	26.05223391157937	24.24989740383767
82-83	23.68359364326362	26.30832884715962	26.113557800213705	23.894519709363056
84-85	23.463604147475596	25.454187303556143	26.89527882925932	24.186929719708942
86-87	23.52948508845862	26.654709818710405	25.967886226804243	23.84791886602673
88-89	23.752067565475503	25.951799063550407	25.795596319194246	24.500537051779844
90-91	23.373915959944423	25.590771884432943	26.376742848929137	24.658569306693497
92-93	23.464163086632826	25.77753735197922	26.509196286794968	24.249103274592986
94-95	23.927842460830817	25.433951415840163	26.58861578266494	24.04959034066408
96-97	23.77777777777778	26.26888984715634	26.36466867902832	23.588663696037564
98-99	22.769203642773604	26.609812413545935	26.44604379971932	24.17494014396114
100-101	23.728924868702865	25.67551632034391	26.127170788856567	24.46838802209665
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.5
2	4.5
3	8.0
4	9.0
5	12.5
6	19.0
7	28.5
8	40.5
9	51.5
10	63.5
11	73.5
12	84.0
13	88.0
14	104.0
15	131.5
16	151.5
17	170.5
18	197.5
19	226.0
20	282.5
21	351.0
22	425.5
23	547.5
24	828.5
25	1229.5
26	1857.0
27	2859.5
28	4252.5
29	6516.0
30	9448.5
31	12287.0
32	15176.5
33	17719.0
34	19590.0
35	21223.0
36	23958.5
37	26104.5
38	25967.5
39	25767.0
40	26033.5
41	26406.5
42	26435.0
43	27052.5
44	28908.0
45	32256.0
46	35983.5
47	36698.0
48	38304.0
49	43045.0
50	47880.0
51	47524.5
52	44931.0
53	46288.0
54	48219.0
55	47278.5
56	39962.0
57	33212.5
58	30900.5
59	28486.5
60	23516.5
61	17855.5
62	14195.5
63	10234.0
64	6417.0
65	4282.5
66	3523.5
67	2947.0
68	2246.5
69	1508.5
70	868.0
71	570.0
72	458.0
73	350.0
74	261.0
75	187.0
76	118.0
77	81.0
78	57.0
79	33.5
80	23.5
81	20.0
82	17.5
83	16.5
84	12.0
85	7.5
86	9.5
87	9.5
88	8.5
89	7.5
90	7.0
91	8.0
92	6.5
93	2.5
94	3.0
95	2.5
96	5.0
97	6.0
98	1.5
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1459441330075224
2	0.0
3	9.582674524459778E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03785156437161612
16-17	0.07440946768243016
18-19	0.025921134588663696
20-21	0.041492980690910834
22-23	0.06789324900579752
24-25	0.018159168223851278
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.024435820037372432
38-39	0.005941258205165062
40-41	0.006372478558765751
42-43	0.0038809831824062097
44-45	0.01240956350917541
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	4.791337262229889E-5
58-59	0.0172009007714053
60-61	0.017823774615495187
62-63	0.03119160557711657
64-65	0.04978199415456854
66-67	0.017919601360739782
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01854247520482967
80-81	0.0599875425231182
82-83	0.05083608835225911
84-85	0.0299937712615591
86-87	0.06065832973983039
88-89	0.03397058118920991
90-91	0.0
92-93	0.002970629102582531
94-95	0.0
96-97	0.0
98-99	4.312203536006899E-4
100-101	0.04753006564132049
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1043550.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.237401767382416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.93358830005329	34.45193212866478
2	15.090246068276416	14.256480325916717
3	5.308175066871457	7.522331948562271
4	2.4227143612668103	4.577709266030703
5	1.2681670922583617	2.9952459222590675
6	0.7253661066042707	2.0558646123664723
7	0.47026358391200496	1.5549820894874373
8	0.3025658553790823	1.1433939897306746
9	0.23040069459806528	0.9795177160391506
>10	1.0075343270697599	8.739345822996158
>50	0.10911202651454949	3.6090172490788914
>100	0.11587026799243928	11.260209815159229
>500	0.012304807079637287	3.862193334727852
>1k	0.003691442123891186	2.9917757789806423
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	3567	0.34181400028748027	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2770	0.26544008432753585	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2524	0.2418667049973648	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	2386	0.22864261415361026	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1932	0.1851372718125629	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	1918	0.1837956973791385	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1644	0.15753916918211874	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1607	0.15399357960806861	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1468	0.14067366201906953	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1386	0.1328158689090125	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1369	0.13118681423985434	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1340	0.128407838627761	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1330	0.12744957117531505	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	1261	0.12083752575343777	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1231	0.11796272339609985	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1085	0.10397201859038858	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1055	0.10109721623305064	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	1.9165349048919553E-4	0.0
26-27	0.0	0.0	0.0	4.312203536006899E-4	0.0
28-29	0.0	0.0	0.0	0.0010061808250682765	0.0
30-31	0.0	0.0	0.0	0.0028748023573379328	0.0
32-33	0.0	0.0	0.0	0.006564132049254948	0.0
34-35	0.0	0.0	0.0	0.010924248957884145	0.0
36-37	0.0	0.0	0.0	0.018734128695318864	0.0
38-39	4.791337262229888E-5	0.0	0.0	0.03627042307508026	0.0
40-41	9.582674524459776E-5	0.0	0.0	0.05629821283120119	0.0
42-43	9.582674524459776E-5	0.0	0.0	0.0768051363135451	0.0
44-45	9.582674524459776E-5	0.0	0.0	0.09841406736620191	0.0
46-47	9.582674524459776E-5	0.0	0.0	0.12634756360500216	0.0
48-49	9.582674524459776E-5	0.0	0.0	0.15653298835705046	0.0
50-51	9.582674524459776E-5	0.0	0.0	0.19055148291888266	0.0
52-53	9.582674524459776E-5	0.0	0.0	0.22849887403574337	0.0
54-55	9.582674524459776E-5	0.0	0.0	0.263379809304777	0.0
56-57	9.582674524459776E-5	0.0	0.0	0.3006564132049255	0.0
58-59	9.582674524459776E-5	0.0	0.0	0.34574289684250875	0.0
60-61	9.582674524459776E-5	0.0	0.0	0.40117866896650856	0.0
62-63	9.582674524459776E-5	0.0	0.0	0.4605912510181592	0.0
64-65	9.582674524459776E-5	0.0	0.0	0.5113315126251736	0.0
66-67	9.582674524459776E-5	0.0	0.0	0.5745771644866082	0.0
68-69	9.582674524459776E-5	0.0	0.0	0.653107182214556	0.0
70-71	9.582674524459776E-5	0.0	0.0	0.7446696372957693	0.0
72-73	9.582674524459776E-5	0.0	0.0	0.845623113410953	0.0
74-75	9.582674524459776E-5	0.0	0.0	0.9429830865794643	0.0
76-77	9.582674524459776E-5	0.0	0.0	1.0462364045805184	0.0
78-79	9.582674524459776E-5	0.0	0.0	1.1645824349575968	0.0
80-81	9.582674524459776E-5	0.0	0.0	1.3078913324708927	0.0
82-83	9.582674524459776E-5	0.0	0.0	1.4577164486608212	0.0
84-85	9.582674524459776E-5	0.0	0.0	1.6110871544248	0.0
86-87	9.582674524459776E-5	0.0	0.0	1.7721239998083465	0.0
88-89	9.582674524459776E-5	0.0	0.0	1.9563030041684635	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACCGT	425	0.0	62.559544	6
GGTATCA	910	0.0	60.103626	1
AACCGTG	480	0.0	57.369522	7
ACCGTGC	520	0.0	54.78519	8
CCGTGCG	515	0.0	53.516777	9
GTATCAA	2000	0.0	49.70047	1
TATCACG	75	6.9434864E-6	37.98258	2
ATCAACG	2860	0.0	34.695625	3
GCGTAAT	55	0.0025635276	34.58928	1
GTCTAAT	1080	0.0	33.46833	1
TCAACGC	3025	0.0	32.646183	4
CTAATGT	1160	0.0	32.33431	3
TAGACCG	145	2.4103429E-8	29.469242	5
CGCAGAG	3420	0.0	28.877028	8
TGCGCGG	475	0.0	28.516994	12-13
CCCATAA	1175	0.0	28.2849	2
ACGCAGA	3500	0.0	28.21563	7
TATCAAC	3585	0.0	28.20882	2
TCTAATG	1305	0.0	28.01397	2
AATGCGG	330	0.0	27.335945	7
>>END_MODULE