FastQCFastQC Report
Fri 10 Feb 2017
SRR3224528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043550
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC25110.24062095730918498No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT22290.2135978151502084No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT21670.20765655694504337No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA21060.2018111254851229No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG19930.19098270327248335No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA19530.18714963346269944No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG16950.1624263331895932No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA16380.15696420871065114No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG16090.1541852330985578No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC14290.1369364189545302No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG14060.13473240381390447No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG13730.13157012122083273No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA13420.1285994921182502No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG12820.12284988740357435No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT12740.12208327344161755No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC12540.12016673853672559No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT12030.11527957452925111No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT11760.11269225240764698No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA11730.11240477217191316No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG11390.10914666283359685No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC10930.10473863255234536No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA10820.10368453835465478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11500.062.629181
AAACCGT4200.056.5327036
AACCGTG4650.053.104277
CCGTGCG4450.052.2895749
ACCGTGC4700.050.5185858
GTATCAA21100.046.341391
GCGTAAT704.188514E-640.8837621
ATCAACG28900.033.6848873
TCAACGC31150.031.2517874
GTACATG49500.029.5822221
TACATGG50200.028.7573552
CGCAGAG35100.027.870148
ACATGGG51450.027.6894863
TATCAAC35600.027.4787042
TGCGCGG4250.027.37513212-13
ACGCAGA36100.027.0981147
AACGCAG36350.026.7811056
CAACGCA36200.026.7608975
GTCTAAT8800.026.558961
ATGCGGG2550.026.0715548
Produced by FastQC (version 0.11.5)