##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3224528_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1043550 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.16484691677447 34.0 33.0 34.0 31.0 34.0 2 33.29265967131426 34.0 34.0 34.0 31.0 34.0 3 33.399599444204874 34.0 34.0 34.0 31.0 34.0 4 36.62847395908198 37.0 37.0 37.0 35.0 37.0 5 36.662259594652866 37.0 37.0 37.0 35.0 37.0 6 36.692930861003305 37.0 37.0 37.0 36.0 37.0 7 36.665021321450816 37.0 37.0 37.0 35.0 37.0 8 36.65424177087825 37.0 37.0 37.0 35.0 37.0 9 38.49250539025442 39.0 39.0 39.0 37.0 39.0 10-11 38.51325858847204 39.0 39.0 39.0 37.5 39.0 12-13 38.49191413923626 39.0 39.0 39.0 37.0 39.0 14-15 40.07793014230272 41.0 40.0 41.0 38.0 41.0 16-17 40.08901442192516 41.0 40.0 41.0 38.0 41.0 18-19 40.08906568923386 41.0 40.0 41.0 38.0 41.0 20-21 40.0880595084088 41.0 40.0 41.0 38.0 41.0 22-23 40.00464136840593 41.0 40.0 41.0 38.0 41.0 24-25 39.94993483781323 41.0 40.0 41.0 38.0 41.0 26-27 39.87726702122562 41.0 40.0 41.0 38.0 41.0 28-29 39.84757462507786 41.0 40.0 41.0 38.0 41.0 30-31 39.76693354415217 41.0 40.0 41.0 37.0 41.0 32-33 39.70144602558574 41.0 40.0 41.0 37.0 41.0 34-35 39.605748646447225 41.0 40.0 41.0 37.0 41.0 36-37 39.473735326529635 41.0 40.0 41.0 36.0 41.0 38-39 39.34853672560011 41.0 39.0 41.0 36.0 41.0 40-41 39.201594077907146 41.0 39.0 41.0 35.0 41.0 42-43 39.08318432274447 41.0 39.0 41.0 35.0 41.0 44-45 38.80865411336304 40.5 38.5 41.0 35.0 41.0 46-47 38.54570456614441 40.0 38.0 41.0 35.0 41.0 48-49 38.41107565521537 40.0 38.0 41.0 35.0 41.0 50-51 38.21500838484021 40.0 37.0 41.0 35.0 41.0 52-53 37.940841837956974 40.0 36.5 41.0 34.0 41.0 54-55 37.72985769728331 39.5 36.0 41.0 34.0 41.0 56-57 37.474712759331126 39.0 35.0 41.0 34.0 41.0 58-59 37.189410665516746 39.0 35.0 41.0 33.5 41.0 60-61 36.925753437784486 38.5 35.0 41.0 33.0 41.0 62-63 36.70347276124767 37.5 35.0 41.0 33.0 41.0 64-65 36.4228704901538 37.0 35.0 40.0 33.0 41.0 66-67 36.14434957596666 36.5 35.0 40.0 33.0 41.0 68-69 35.88385510996119 36.0 35.0 39.5 32.0 41.0 70-71 35.59255042882469 35.5 35.0 39.0 32.0 41.0 72-73 35.29702457956016 35.0 35.0 39.0 32.0 41.0 74-75 35.00448852474726 35.0 35.0 37.5 31.0 40.0 76-77 33.21080781946241 34.5 32.0 35.5 28.5 38.5 78-79 34.53520099659815 35.0 34.5 37.0 31.5 39.0 80-81 34.48555028508457 35.0 35.0 36.0 32.0 39.0 82-83 34.28734032868574 35.0 35.0 36.0 32.0 37.5 84-85 34.09378611470461 35.0 35.0 36.0 32.0 37.0 86-87 33.906626419433664 35.0 35.0 35.0 31.0 37.0 88-89 33.76232715250826 35.0 34.5 35.0 31.0 36.0 90-91 33.623529778161085 35.0 34.0 35.0 31.0 36.0 92-93 33.521832686502805 35.0 34.0 35.0 31.0 36.0 94-95 33.426638876910545 35.0 34.0 35.0 31.0 36.0 96-97 33.38407311580662 35.0 34.0 35.0 31.0 35.5 98-99 33.35032772746874 35.0 34.0 35.0 31.0 35.0 100-101 32.50724450194049 34.5 33.0 35.0 27.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 10.0 14 18.0 15 61.0 16 232.0 17 556.0 18 973.0 19 1563.0 20 2067.0 21 2950.0 22 4071.0 23 3820.0 24 3134.0 25 3262.0 26 4050.0 27 5464.0 28 6772.0 29 7962.0 30 8552.0 31 9358.0 32 11141.0 33 15413.0 34 26139.0 35 52844.0 36 147619.0 37 300241.0 38 317521.0 39 107718.0 40 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57656698413738 19.99872921863312 13.742345227012576 23.682358570216927 2 15.598102630444158 20.35312155622634 39.29864405155479 24.75013176177471 3 17.74155526807532 28.425662402376506 29.463370226630254 24.369412102917927 4 12.119495951320014 17.587465861722006 40.29284653346749 30.00019165349049 5 12.885439126060083 39.07191797230607 35.13334291600786 12.909299985625989 6 28.304633223132576 38.42278760001916 19.902256719850513 13.37032245699775 7 25.505533994537878 32.05941258205165 24.2359254467922 18.199127976618275 8 25.930717263188157 33.98553016146806 21.231948636864548 18.851803938479232 9 26.71802980211777 14.95395524890997 21.298931531790522 37.02908341718174 10-11 26.022040151406255 25.177183651957264 27.933592065545493 20.867184131090987 12-13 26.313736763930812 23.350677974222606 28.436347084471276 21.899238177375306 14-15 23.731397633079393 25.122849887403575 25.57251688946385 25.573235590053184 16-17 22.82492453643812 27.354654784150256 26.735278616261798 23.085142063149824 18-19 22.89765703607877 26.724737674284892 27.928321594557044 22.4492836950793 20-21 24.585306580856354 25.21795960296759 27.218166590324106 22.978567225851954 22-23 24.533032690586484 26.067853521947367 26.458637984863092 22.940475802603057 24-25 23.848180960092762 25.81374066283677 26.81427250382111 23.523805873249355 26-27 23.439383630561654 26.675658581928648 27.045029850603242 22.839927936906463 28-29 23.3691865841633 26.595643753360044 27.40071278460355 22.634456877873106 30-31 23.331135683477093 25.741970352809044 27.503380324070303 23.42351363964356 32-33 23.494274351971637 25.835177998179294 27.75348569785827 22.9170619519908 34-35 23.95648909424312 25.772813542007565 26.9088847862763 23.361812577473014 36-37 23.17645632661236 25.775772394419107 27.588762889611118 23.459008389357415 38-39 23.33951415840161 26.063197738488814 26.904604475109 23.692683628000573 40-41 23.55713669685209 25.924871831728236 27.350677974222602 23.167313497197068 42-43 23.681376072061713 26.179052273489532 27.28369507929663 22.855876575152127 44-45 23.85856585923095 25.716504962298796 26.955546312872077 23.469382865598178 46-47 23.590116369672824 25.687925585552495 26.56445021335319 24.15750783142149 48-49 23.42954558195226 25.61210912626067 27.074482280805732 23.883863010981337 50-51 23.731301806334148 26.00574960471468 26.368310095347613 23.894638493603566 52-53 24.023489063048757 26.16047441927767 25.927519489971484 23.88851702770209 54-55 23.793110057016914 25.503473719515117 26.52139332087586 24.182022902592113 56-57 23.959774347641293 26.175184853135736 25.869663864620087 23.995376934602888 58-59 24.431568437314706 25.96985077817664 26.155880747168446 23.44270003734021 60-61 23.870089261750113 26.27388748725106 26.224377506652452 23.631645744346372 62-63 23.76177471132193 25.93646686790283 26.608308178812706 23.693450241962534 64-65 24.280436969958316 25.610847587561686 26.08835225911552 24.020363183364477 66-67 23.651717694408507 25.75564179962628 26.083177614872312 24.5094628910929 68-69 23.87125676776388 25.98941114465047 26.62800057496047 23.511331512625176 70-71 24.324661012888697 25.48809352690336 26.420296104642805 23.76694935556514 72-73 23.96123808154856 25.759235302572947 26.512529346940735 23.76699726893776 74-75 24.00019165349049 25.51233769345024 26.548943510133675 23.938527142925594 76-77 23.92110753037477 24.959393299966365 26.23580921606789 24.883689953590974 78-79 23.837265856315046 25.02744484317267 26.50527357872609 24.630015721786197 80-81 23.390028184509347 26.03564415643409 26.38931614471179 24.185011514344776 82-83 23.56697328236594 26.02154391337971 26.55257138421704 23.858911420037316 84-85 23.551421662085197 25.408319673564673 26.996989500385478 24.04326916396466 86-87 23.312826409850988 26.473575774998803 26.384361075176084 23.829236739974128 88-89 23.76239758516602 25.733074601121174 25.748454793732932 24.756073019979876 90-91 23.698960279814095 25.655407024100423 26.215993483781325 24.429639212304156 92-93 23.74233325778988 25.79935115679707 26.494766180233903 23.963549405179148 94-95 23.84471275933113 25.46121412486225 26.340137032245696 24.353936083560924 96-97 23.73992621340616 26.16879881174836 26.409276028939676 23.681998945905804 98-99 22.422548033156055 26.702601696133392 26.67284749173494 24.20200277897561 100-101 23.39109059238628 25.823914755981615 26.31545637568125 24.469538275950853 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 1.0 2 6.0 3 9.5 4 4.5 5 6.0 6 15.0 7 28.5 8 49.5 9 68.5 10 78.0 11 82.0 12 94.0 13 120.5 14 138.0 15 153.0 16 171.5 17 202.5 18 235.5 19 254.5 20 295.0 21 390.0 22 498.5 23 633.0 24 885.0 25 1304.0 26 1970.5 27 3068.0 28 4468.0 29 6655.5 30 9527.5 31 12234.0 32 15164.0 33 17677.0 34 19450.0 35 21026.0 36 23822.5 37 26005.0 38 25985.5 39 26252.0 40 26519.5 41 26417.5 42 26677.0 43 27821.0 44 29727.5 45 32556.5 46 34905.5 47 36766.5 48 39103.5 49 43494.0 50 48783.0 51 47486.5 52 43677.5 53 45270.0 54 48247.0 55 47653.0 56 40502.0 57 33583.5 58 31312.5 59 29646.0 60 24344.0 61 17325.0 62 13427.5 63 9515.5 64 5660.0 65 3618.0 66 2947.0 67 2454.0 68 1821.0 69 1180.0 70 656.5 71 373.5 72 289.5 73 244.0 74 180.5 75 117.0 76 82.5 77 51.0 78 32.0 79 16.5 80 9.5 81 8.0 82 5.0 83 3.5 84 3.5 85 1.5 86 1.0 87 1.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4617890853337166 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 7.666139619567822E-4 22-23 7.666139619567822E-4 24-25 4.7913372622298883E-4 26-27 0.0019165349048919553 28-29 0.0035455895740501174 30-31 0.0010540941976905755 32-33 0.0 34-35 0.003976809927650807 36-37 0.002443582003737243 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.007857793110057017 46-47 0.07623017584207753 48-49 6.228738440898855E-4 50-51 0.0 52-53 4.791337262229889E-5 54-55 0.0 56-57 0.008289013463657707 58-59 0.04202002778975612 60-61 0.03123951894973887 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 2.8748023573379333E-4 78-79 0.008959800680369891 80-81 0.006612045421877245 82-83 6.707872167121844E-4 84-85 0.0034018494561832207 86-87 0.0 88-89 0.0 90-91 0.0 92-93 4.791337262229889E-5 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.06789324900579752 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1043550.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.3432950732471 #Duplication Level Percentage of deduplicated Percentage of total 1 71.35710521581072 32.35566277373245 2 15.710824898089879 14.24761138396413 3 5.785168762643235 7.869558427591919 4 2.5207616941096562 4.571985652214096 5 1.36155082626505 3.086860043627977 6 0.8123937955471481 2.21019669523017 7 0.5008427605901942 1.589690275511858 8 0.3525476400138855 1.2788537334821204 9 0.24083027893293893 0.9828034560205787 >10 1.099882904173345 9.12861019428443 >50 0.11729937242227911 3.7655948085661186 >100 0.12406620301013778 11.92179333213249 >500 0.011856155821863792 3.6152449812982677 >1k 0.004869492569694057 3.3755342423433894 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC 2511 0.24062095730918498 No Hit ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT 2229 0.2135978151502084 No Hit GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT 2167 0.20765655694504337 No Hit ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA 2106 0.2018111254851229 No Hit TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG 1993 0.19098270327248335 No Hit GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA 1953 0.18714963346269944 No Hit CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG 1695 0.1624263331895932 No Hit TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA 1638 0.15696420871065114 No Hit TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG 1609 0.1541852330985578 No Hit GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC 1429 0.1369364189545302 No Hit GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG 1406 0.13473240381390447 No Hit ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG 1373 0.13157012122083273 No Hit ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA 1342 0.1285994921182502 No Hit GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG 1282 0.12284988740357435 No Hit GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT 1274 0.12208327344161755 No Hit GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC 1254 0.12016673853672559 No Hit GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT 1203 0.11527957452925111 No Hit ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT 1176 0.11269225240764698 No Hit CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA 1173 0.11240477217191316 No Hit GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG 1139 0.10914666283359685 No Hit TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC 1093 0.10473863255234536 No Hit GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA 1082 0.10368453835465478 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 1.9165349048919553E-4 0.0 26-27 0.0 0.0 0.0 3.8330698097839105E-4 0.0 28-29 0.0 0.0 0.0 9.103540798236787E-4 0.0 30-31 0.0 0.0 0.0 0.0026352354942264385 0.0 32-33 0.0 0.0 0.0 0.006659958794499545 0.0 34-35 0.0 0.0 0.0 0.011115902448373342 0.0 36-37 0.0 0.0 0.0 0.018973695558430356 0.0 38-39 0.0 0.0 0.0 0.03689329691917014 0.0 40-41 0.0 0.0 0.0 0.05725648028364717 0.0 42-43 0.0 0.0 0.0 0.07766757702074649 0.0 44-45 0.0 0.0 0.0 0.09956398830913708 0.0 46-47 0.0 0.0 0.0 0.12759331129318194 0.0 48-49 0.0 0.0 0.0 0.1579703895357194 0.0 50-51 0.0 0.0 0.0 0.19184514397968472 0.0 52-53 0.0 0.0 0.0 0.22984044846916774 0.0 54-55 0.0 0.0 0.0 0.2650567773465574 0.0 56-57 0.0 0.0 0.0 0.3027646016003066 0.0 58-59 0.0 0.0 0.0 0.3491926596713143 0.0 60-61 0.0 0.0 0.0 0.40515547889415937 0.0 62-63 0.0 0.0 0.0 0.4652867615351445 0.0 64-65 0.0 0.0 0.0 0.5168894638493604 0.0 66-67 0.0 0.0 0.0 0.5811412965358631 0.0 68-69 0.0 0.0 0.0 0.6602462747352786 0.0 70-71 0.0 0.0 0.0 0.7529107373868047 0.0 72-73 0.0 0.0 0.0 0.856355708878348 0.0 74-75 0.0 0.0 0.0 0.9555363902065066 0.0 76-77 0.0 0.0 0.0 1.0609937233481865 0.0 78-79 0.0 0.0 0.0 1.1805855014134443 0.0 80-81 0.0 0.0 0.0 1.3253797134780316 0.0 82-83 0.0 0.0 0.0 1.4771213645728523 0.0 84-85 0.0 0.0 0.0 1.6325523453595898 0.0 86-87 0.0 0.0 0.0 1.796895213454075 0.0 88-89 0.0 0.0 0.0 1.9836615399357962 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1150 0.0 62.62918 1 AAACCGT 420 0.0 56.532703 6 AACCGTG 465 0.0 53.10427 7 CCGTGCG 445 0.0 52.289574 9 ACCGTGC 470 0.0 50.518585 8 GTATCAA 2110 0.0 46.34139 1 GCGTAAT 70 4.188514E-6 40.883762 1 ATCAACG 2890 0.0 33.684887 3 TCAACGC 3115 0.0 31.251787 4 GTACATG 4950 0.0 29.582222 1 TACATGG 5020 0.0 28.757355 2 CGCAGAG 3510 0.0 27.87014 8 ACATGGG 5145 0.0 27.689486 3 TATCAAC 3560 0.0 27.478704 2 TGCGCGG 425 0.0 27.375132 12-13 ACGCAGA 3610 0.0 27.098114 7 AACGCAG 3635 0.0 26.781105 6 CAACGCA 3620 0.0 26.760897 5 GTCTAAT 880 0.0 26.55896 1 ATGCGGG 255 0.0 26.071554 8 >>END_MODULE