FastQCFastQC Report
Fri 10 Feb 2017
SRR3224526_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224526_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412753
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG53150.3762157999310566No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA53130.37607423236758303No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA49770.35229088170402045No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA42670.3020343966708972No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG31920.22594183130384432No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA31510.2230396962526358No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC31240.22112853414574238No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA31110.22020834498316408No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA30130.21327153437295832No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA29900.2116435073930121No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA29300.2073964804888045No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC28070.1986900753351789No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC27960.19791145373607416No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT27320.1933812917049194No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG26380.18672761622166084No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA25690.18184353528182207No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC24360.17242929231082857No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC22190.15706921167394441No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT20860.1476549687029509No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA19420.13746210413285267No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG19420.13746210413285267No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT19330.1368250500972215No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG18460.13066686108612052No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG17820.12613669905496575No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG17740.1255704288010714No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC17210.12181888836902133No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC17210.12181888836902133No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG17170.12153575324207416No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC16980.1201908613890751No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT16660.1179257803734977No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC16330.11558991557618352No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG16300.11537756423097313No Hit
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC16210.11474051019534201No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA16020.11339561834234292No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA15720.11127210489023914No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT15330.10851153740250419No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG15230.10780369958513626No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC14920.10560940235129566No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG14770.10454764562524375No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA14600.10334432133571828No Hit
ATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGT14570.10313196999050789No Hit
GTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATC14340.10150394301056163No Hit
CCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATC14150.10015905115756257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21550.055.8344081
GGTATCA9550.053.7834971
CCGTGCG3100.039.8510869
ATCAACG29750.039.734873
AACCGTG3300.035.965457
AAACCGT3300.035.965456
TCAACGC33250.035.6950344
CGCAGAG37000.031.8274358
GCGTAAT600.00392749731.70571
ACGCAGA37850.031.2315047
GTACATG63900.030.887011
TACATGG64950.030.4800972
ACCGTGC4850.030.3509048
TATCAAC39900.030.1028082
CCCATAA21350.028.6847612
ACATGGG67350.028.6185673
CATAATA21800.028.528194
CCATAAT21900.028.3979263
GCCCATA21750.028.2071421
GTCTAAT7200.027.742491
Produced by FastQC (version 0.11.5)